FlyBase Allele Report: Dmel\trio[UAS.cBa]

General Information

Symbol

Dmel\trio^UAS.cBa

Species

D. melanogaster

Name

Saccharomyces cerevisiae UAS construct a of Bateman

FlyBase ID

FBal0117278

Feature type

allele

Associated gene

Dmel\trio

Associated Insertion(s)

Carried in Construct

P{UAS-trio.B}

Also Known As

UAS-Trio

Key Links

Transgenic product class

cDNA

(Bateman et al., 2000)

wild_type

(Bateman et al., 2000)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

cDNA

(Bateman et al., 2000)

wild_type

(Bateman et al., 2000)

Mutagen

in vitro construct

(Bateman et al., 2000)

Progenitor genotype

Carried in construct

P{UAS-trio.B}

Cytology

Description

Full length wild type trio cDNA is expressed under the influence of UAS regulatory sequences.

(Bateman et al., 2000)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

trio

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

visible, with Scer\GAL4^GMR.PFa

(Lee and Thomas, 2011)

Phenotype Manifest In

eye, with Scer\GAL4^GMR.PFa

(Lee and Thomas, 2011)

ommatidium, with Scer\GAL4^GMR.PFa

(Lee and Thomas, 2011)

Detailed Description

Statement

Reference

Overexpression of a single copy of trio^Scer\UAS.cBa during eye development driven by Scer\GAL4^GMR.PFa causes a mild rough-eye phenotype, and expression of two copies results in a reduced number of ommatidia and a smaller bar shaped eye.

(Lee and Thomas, 2011)

Pan-neuronal expression of trio^Scer\UAS.cBa under the control of Scer\GAL4^elav-C155 does not produce any dominant axon patterning defects in a wild-type background.

(Song and Giniger, 2011)

Third instar larvae expressing trio^Scer\UAS.cBa in motor neurons under the control of Scer\GAL4^BG380 show a similar number of boutons per muscle surface area as in wild type.

(Ball et al., 2010)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Scer\GAL4^GMR.PFa, trio^UAS.cBa has visible phenotype, enhanceable by kst[+]/kst¹

(Lee and Thomas, 2011)

Scer\GAL4^GMR.PFa, trio^UAS.cBa has visible phenotype, enhanceable by kst[+]/kst²

(Lee and Thomas, 2011)

Scer\GAL4^GMR.PFa, trio^UAS.cBa has visible phenotype, enhanceable by kst[+]/kst^B1-14.1

(Lee and Thomas, 2011)

NOT Enhanced by

Statement

Reference

Scer\GAL4^GMR.PFa, trio^UAS.cBa has visible phenotype, non-enhanceable by kst^Δ.UAS/Scer\GAL4^GMR.PFa

(Lee and Thomas, 2011)

NOT suppressed by

Statement

Reference

Scer\GAL4^GMR.PFa, trio^UAS.cBa has visible phenotype, non-suppressible by kst^Δ.UAS/Scer\GAL4^GMR.PFa

(Lee and Thomas, 2011)

Enhancer of

Statement

Reference

trio^UAS.cBa/Scer\GAL4^GMR.PFa is an enhancer of visible phenotype of Scer\GAL4^GMR.PFa, kst^GD1472

(Lee and Thomas, 2011)

Scer\GAL4^elav-C155/trio^UAS.cBa is an enhancer of abnormal neuroanatomy | heat sensitive phenotype of N^l1N-ts1, trio^123.4/trio[+]

(Song and Giniger, 2011)

Suppressor of

Statement

Reference

Scer\GAL4^Act.PU/trio^UAS.cBa is a suppressor | partially | somatic clone of abnormal neuroanatomy | somatic clone | adult stage phenotype of RhoGAP100F^CD

(Holbrook et al., 2012)

Scer\GAL4^Act.PU/trio^UAS.cBa is a suppressor of abnormal neuroanatomy | somatic clone | adult stage phenotype of Lar²¹²⁷

(Holbrook et al., 2012)

trio^UAS.cBa/Scer\GAL4^GMR.PFa is a suppressor of visible phenotype of Scer\GAL4^GMR.PFa, kst^βH33.UAS

(Lee and Thomas, 2011)

trio^UAS.cBa/Scer\GAL4^ey.200.Exel is a suppressor of visible phenotype of Scer\GAL4^ey.200.Exel, kst^βH33.UAS

(Lee and Thomas, 2011)

Scer\GAL4^BG380/trio^UAS.cBa is a suppressor | partially of abnormal neuroanatomy | third instar larval stage phenotype of Mad^k00237/Mad¹

(Ball et al., 2010)

Scer\GAL4^BG380/trio^UAS.cBa is a suppressor | partially of abnormal neuroanatomy | third instar larval stage phenotype of wit^HA3/wit^A12

(Ball et al., 2010)

NOT Suppressor of

Statement

Reference

trio^UAS.cBa/Scer\GAL4^arm.PS is a non-suppressor of partially lethal - majority die | male limited | pupal stage phenotype of Vav²

(Malartre et al., 2010)

trio^UAS.cBa/Scer\GAL4^αTub84B.PL is a non-suppressor of partially lethal - majority die | male limited | pupal stage phenotype of Vav²

(Malartre et al., 2010)

trio^UAS.cBa/Scer\GAL4^arm.PS is a non-suppressor of partially lethal - majority die phenotype of Vav³

(Malartre et al., 2010)

trio^UAS.cBa/Scer\GAL4^αTub84B.PL is a non-suppressor of partially lethal - majority die phenotype of Vav³

(Malartre et al., 2010)

Phenotype Manifest In

Enhanced by

Statement

Reference

Scer\GAL4^GMR.PFa, trio^UAS.cBa has eye phenotype, enhanceable by kst[+]/kst¹

(Lee and Thomas, 2011)

Scer\GAL4^GMR.PFa, trio^UAS.cBa has eye phenotype, enhanceable by kst[+]/kst²

(Lee and Thomas, 2011)

Scer\GAL4^GMR.PFa, trio^UAS.cBa has eye phenotype, enhanceable by kst[+]/kst^B1-14.1

(Lee and Thomas, 2011)

NOT Enhanced by

Statement

Reference

Scer\GAL4^GMR.PFa, trio^UAS.cBa has eye phenotype, non-enhanceable by kst^Δ.UAS/Scer\GAL4^GMR.PFa

(Lee and Thomas, 2011)

NOT suppressed by

Statement

Reference

Scer\GAL4^GMR.PFa, trio^UAS.cBa has eye phenotype, non-suppressible by kst^Δ.UAS/Scer\GAL4^GMR.PFa

(Lee and Thomas, 2011)

Enhancer of

Statement

Reference

trio^UAS.cBa/Scer\GAL4^GMR.PFa is an enhancer of eye phenotype of Scer\GAL4^GMR.PFa, kst^GD1472

(Lee and Thomas, 2011)

Scer\GAL4^elav-C155/trio^UAS.cBa is an enhancer of larval intersegmental nerve | heat sensitive phenotype of N^l1N-ts1, trio^123.4/trio[+]

(Song and Giniger, 2011)

Scer\GAL4^elav-C155/trio^UAS.cBa is an enhancer of larval intersegmental nerve branch ISNb of A1-7 | heat sensitive phenotype of N^l1N-ts1, trio^123.4/trio[+]

(Song and Giniger, 2011)

Suppressor of

Statement

Reference

Scer\GAL4^elav.PU/trio^UAS.cBa is a suppressor of larval intersegmental nerve branch ISNb of A1-7 | embryonic stage phenotype of Abl⁴

(Kannan et al., 2017)

Scer\GAL4^Act.PU/trio^UAS.cBa is a suppressor | partially | somatic clone of photoreceptor cell R7 | somatic clone phenotype of RhoGAP100F^CD

(Holbrook et al., 2012)

Scer\GAL4^Act.PU/trio^UAS.cBa is a suppressor of photoreceptor cell R7 | somatic clone phenotype of Lar²¹²⁷

(Holbrook et al., 2012)

trio^UAS.cBa/Scer\GAL4^GMR.PFa is a suppressor of eye phenotype of Scer\GAL4^GMR.PFa, kst^βH33.UAS

(Lee and Thomas, 2011)

trio^UAS.cBa/Scer\GAL4^ey.200.Exel is a suppressor of eye phenotype of Scer\GAL4^ey.200.Exel, kst^βH33.UAS

(Lee and Thomas, 2011)

trio^UAS.cBa/Scer\GAL4^ey.200.Exel is a suppressor of macrochaeta | ectopic phenotype of Scer\GAL4^ey.200.Exel, kst^βH33.UAS

(Lee and Thomas, 2011)

Scer\GAL4^elav-C155/trio^UAS.cBa is a suppressor of NMJ bouton | larval stage phenotype of Liprin-α^oos

(Astigarraga et al., 2010)

Scer\GAL4^how-24B/trio^UAS.cBa is a suppressor of NMJ bouton | larval stage phenotype of Liprin-β^Δ83

(Astigarraga et al., 2010)

Scer\GAL4^elav-C155/trio^UAS.cBa is a suppressor of NMJ bouton | larval stage phenotype of Liprin-β^Δ83

(Astigarraga et al., 2010)

Scer\GAL4^BG380/trio^UAS.cBa is a suppressor | partially of embryonic/larval neuromuscular junction | third instar larval stage phenotype of Mad^k00237/Mad¹

(Ball et al., 2010)

Scer\GAL4^BG380/trio^UAS.cBa is a suppressor | partially of NMJ bouton | third instar larval stage phenotype of Mad^k00237/Mad¹

(Ball et al., 2010)

Scer\GAL4^BG380/trio^UAS.cBa is a suppressor | partially of embryonic/larval neuromuscular junction | third instar larval stage phenotype of wit^HA3/wit^A12

(Ball et al., 2010)

Scer\GAL4^BG380/trio^UAS.cBa is a suppressor | partially of NMJ bouton | third instar larval stage phenotype of wit^HA3/wit^A12

(Ball et al., 2010)

trio^UAS.cBa, Scer\GAL4^slbo.2.6 is a suppressor | partially of border follicle cell phenotype of Rac1^N17.UAS, Scer\GAL4^slbo.2.6

(Geisbrecht and Montell, 2004)

trio^UAS.cBa, Scer\GAL4^elav-C155 is a suppressor of larval intersegmental nerve phenotype of Rac1^N17.UAS, Scer\GAL4^elav-C155

(Bateman et al., 2000)

Additional Comments

Genetic Interactions

Statement

Reference

The stalling of the ISNb motor nerve at the junction of muscles 6 and 13 with failure to innervate muscle 12 characteristic for Abl⁴ homozygous mutant embryos is not suppressed by Scer\GAL4^elav.PU-driven expression of trio^Scer\UAS.cBa in the mutant background.

(Kannan et al., 2017)

Expression of trio^Scer\UAS.cBa under the control of Scer\GAL4^Act.PU in RhoGAP100F^CD R7 photoreceptor cell clones almost completely suppresses the failure of the axon terminals to contact the M6 layer of the medulla. The later tendency for R7 axons that do reach the M6 layer to project extensions is partially suppressed.

Expression of trio^Scer\UAS.cBa under the control of Scer\GAL4^Act.PU in Lar²¹²⁷ R7 photoreceptor cell clones partially suppresses the failure of the axon terminals to contact the M6 layer of the medulla.

(Holbrook et al., 2012)

Heterozygosity for kst¹ significantly enhances the rough-eye phenotype induced by overexpression of trio^Scer\UAS.cBa via Scer\GAL4^GMR.PFa.

Heterozygosity for kst² significantly enhances the rough-eye phenotype induced by overexpression of trio^Scer\UAS.cBa via Scer\GAL4^GMR.PFa.

Heterozygosity for kst^B1-14.1 significantly enhances the rough-eye phenotype induced by overexpression of trio^Scer\UAS.cBa via Scer\GAL4^GMR.PFa.

Co-expression of kst^Δ.Scer\UAS with trio^Scer\UAS.cBa under the control of Scer\GAL4^GMR.PFa does not modify the eye phenotype of trio^Scer\UAS.cBa-overexpression alone.

Co-expression of kst^{βH33.Scer\UAS} and trio^Scer\UAS.cBa under the control of Scer\GAL4^GMR.PFa suppresses the roughness of both the kst^{βH33.Scer\UAS} and the trio^Scer\UAS.cBa eye phenotype.

Co-expression of trio^Scer\UAS.cBa with kst^{βH33.Scer\UAS} under the control of Scer\GAL4^ey.200.Exel suppresses the eye phenotype resulting from the overexpression of kst^{βH33.Scer\UAS}.

(Lee and Thomas, 2011)

A trio^123.4 heterozygous background significantly restores the ability of ISNb axons to enter the ventrolateral muscle field in N^l1N-ts1 mutants. This suppression is reverted upon pan-neural expression of trio^Scer\UAS.cBa under the control of Scer\GAL4^elav-C155.

(Song and Giniger, 2011)

Scer\GAL4^how-24B- or Scer\GAL4^elav-C155-mediated expression of trio^Scer\UAS.cBa restores the number of boutons in Liprin-β^Δ83 animals to control levels.

Scer\GAL4^elav-C155-mediated expression of trio^Scer\UAS.cBa restores the number of boutons in Liprin-α^oos animals to control levels.

(Astigarraga et al., 2010)

Expression of trio^Scer\UAS.cBa in motor neurons under the control of Scer\GAL4^BG380 partially suppresses the reduced bouton number phenotype seen in Mad¹/Mad^k00237 larval neuromuscular junctions.

Expression of trio^Scer\UAS.cBa in motor neurons under the control of Scer\GAL4^BG380 partially suppresses the reduced bouton number phenotype seen in wit^A12/wit^HA3 larval neuromuscular junctions.

(Ball et al., 2010)

Expression of trio^Scer\UAS.cBa under the control of either the Scer\GAL4^arm.PS or the Scer\GAL4^αTub84B.PL driver cannot suppress the semi-lethality seen in vav² male homozygotes.

Expression of trio^Scer\UAS.cBa under the control of either the Scer\GAL4^arm.PS or the Scer\GAL4^αTub84B.PL driver cannot suppress the semi-lethality seen in vav³ male homozygotes.

(Malartre et al., 2010)

The border follicle cell migration defect seen in egg chambers expressing Rac1^N17.Scer\UAS under the control of Scer\GAL4^slbo.2.6 is partially suppressed by coexpression of trio^Scer\UAS.cBa.

(Geisbrecht and Montell, 2004)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescues

trio^UAS.cBa/Scer\GAL4^RapGAP1-OK6 rescues trio^6A/trio^S137203

(Ball et al., 2010)

Scer\GAL4^{elav.Switch.PO}/trio^UAS.cBa rescues trio^6A/trio^S137203

(Ball et al., 2010)

Partially rescues

Scer\GAL4^T155/trio^UAS.cBa partially rescues trio^S138606/Df(3L)Ar12-1

(Bateman et al., 2000)

Scer\GAL4^elav-C155/trio^UAS.cBa partially rescues trio^6A/Df(3L)Ar12-1

(Bateman et al., 2000)

Scer\GAL4^elav-C155/trio^UAS.cBa partially rescues trio^S137203/trio^BX1B

(Bateman et al., 2000)

Fails to rescue

Scer\GAL4^G14/trio^UAS.cBa fails to rescue trio^6A/trio^S137203

(Ball et al., 2010)

Comments

Expression of trio^Scer\UAS.cBa in neurons under the control of Scer\GAL4^OK6 rescues the reduction in synaptic bouton phenotype seen in trio^S137203/trio^6A third instar larvae. Expression of trio^Scer\UAS.cBa in muscles using Scer\GAL4^G14 is unable to rescue the phenotype.

Expression of trio^Scer\UAS.cBa only in the larval stages using the neuronal driver Scer\GAL4^{elav.Switch.PO} rescues the reduction in synaptic bouton phenotype seen in trio^S137203/trio^6A third instar larvae.

(Ball et al., 2010)

When expression is driven by Scer\GAL4^T155, wing posture of trio^S138606/Df(3L)Ar12-1 is substantially rescued. When expression is driven by Scer\GAL4^elav-C155, larval lethality is rescued to pupal lethality. When expression is driven by Scer\GAL4^elav-C155, short stop phenotype of ISNb is reduced and short stop phenotype of SNa is eliminated.

(Bateman et al., 2000)

Images (0)

Mutant

Wild-type

Stocks (2)

Bloomington

9513

w^*; P{UAS-trio.B}2B

9512

P{UAS-trio.B}1B, y¹ w^*

Notes on Origin

Discoverer