FlyBase Allele Report: Dmel\Mad[k00237]

General Information

Symbol

Dmel\Mad^k00237

Species

D. melanogaster

Name

FlyBase ID

FBal0064630

Feature type

allele

Associated gene

Dmel\Mad

Associated Insertion(s)

P{lacW}Mad^k00237

Carried in Construct

Also Known As

Mad²³⁷, k00237

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

P-element activity

Nature of the Allele

Allele class

Mutagen

P-element activity

(BDGP Project Members, 1994-1999)

Progenitor genotype

Associated Insertion(s)

P{lacW}Mad^k00237

Cytology

Description

Allele components

Component

Use(s)

Inserted element

P{lacW}

enhancer trap

Encoded product / tool

lacZ

reporter enzyme

Tagged with

Tag:NLS(P)

nuclear protein localization tag

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

transposable_element_insertion_site

2L:3,159,608..3,159,608 [+]

(Sharma et al., 2022, Ho and Treisman, 2020, Dworkin and Gibson, 2006, Spradling et al., 1999, BDGP Project Members, 1994-1999)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

Detailed Description

Statement

Reference

Mad¹²/Mad^k00237 transheterozygotes show a significant reduction in the number of synaptic boutons at the third instar larval neuromuscular junction, as compared to controls.

(Banerjee et al., 2017)

Mad^k00237 mutants exhibit undergrown synapses with few boutons on neuromuscular junction 4.

(Liu et al., 2014)

Mad¹/Mad^k00237 third instar larvae show a reduced number of boutons per muscle surface area in muscle 4 compared to controls.

Homozygous Mad^k00237 third instar larvae show a reduced number of boutons per muscle surface area in muscle 4 compared to controls.

(Ball et al., 2010)

The number of boutons per muscle surface area is reduced at the neuromuscular junction in Mad^k00237 third instar larvae.

Homozygous third instar larvae show a severe reduction in excitatory junctional current amplitude and in quantal content at the neuromuscular junction compared to wild type.

(Merino et al., 2009)

Heterozygotes show a direct effect on the shape of the wing.

(Dworkin and Gibson, 2006)

Mad^k00237/Mad^apg larvae transferred to 29^oC at the equivalent of 48 hours, 72 hours, 84 hours or 96 hours after egg laying develop at the same rate as wild-type siblings.

(Galindo et al., 2002)

Removal of Mad function before 72 hours after egg laying in Mad^apg/Mad^k00237 flies produces truncations of the proximal-distal axis in the legs, whereas removal of Mad function from 84 hours after egg laying until the end of development results in a normal proximal-distal organisation in the leg despite dorsal patterning defects.

(Galindo et al., 2002)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Mad^k00237 has abnormal neuroanatomy | third instar larval stage phenotype, enhanceable by trio^S137203

(Ball et al., 2010)

Suppressed by

Statement

Reference

Mad^k00237/Mad¹ has abnormal neuroanatomy | third instar larval stage phenotype, suppressible | partially by Scer\GAL4^BG380/trio^UAS.cBa

(Ball et al., 2010)

NOT suppressed by

Statement

Reference

Mad^k00237/Mad¹ has abnormal size | third instar larval stage phenotype, non-suppressible by Scer\GAL4^BG380/Rac1^UAS.Tag:MYC

(Ball et al., 2010)

Mad^k00237/Mad¹ has abnormal neuroanatomy | third instar larval stage phenotype, non-suppressible by Scer\GAL4^BG380/Rac1^UAS.Tag:MYC

(Ball et al., 2010)

Suppressor of

Statement

Reference

Mad^k00237/Mad¹ is a suppressor of abnormal size | third instar larval stage phenotype of Rac1^UAS.Tag:MYC, Scer\GAL4^BG380

(Ball et al., 2010)

Mad^k00237/Mad¹ is a suppressor of abnormal neuroanatomy | third instar larval stage phenotype of Rac1^UAS.Tag:MYC, Scer\GAL4^{elav.Switch.PO}

(Ball et al., 2010)

Mad^k00237 is a suppressor of visible phenotype of upd1^GMR.PB

(Bach et al., 2003)

NOT Suppressor of

Statement

Reference

Mad^k00237/Mad¹ is a non-suppressor of abnormal size | third instar larval stage phenotype of Rac1^V12.UAS, Scer\GAL4^BG380

(Ball et al., 2010)

Mad^k00237/Mad¹ is a non-suppressor of abnormal neuroanatomy | third instar larval stage phenotype of Rac1^V12.UAS, Scer\GAL4^BG380

(Ball et al., 2010)

Other

Statement

Reference

Mad^k00237, Rab11^93Bi has abnormal neuroanatomy | larval stage phenotype

(Liu et al., 2014)

+/Df(3L)Exel6279, Mad^k00237 has abnormal neuroanatomy | dominant | third instar larval stage phenotype

(Merino et al., 2009)

Mad^k00237/Mad[+], nmo^P1 has abnormal neuroanatomy | dominant | third instar larval stage phenotype

(Merino et al., 2009)

Phenotype Manifest In

NOT Enhanced by

Statement

Reference

Mad^k00237/Mad¹² has neuromuscular junction | third instar larval stage phenotype, non-enhanceable by Nrx-1²⁷³/Nrx-1²⁷³

(Banerjee et al., 2017)

Mad^k00237/Mad¹² has terminal bouton | third instar larval stage phenotype, non-enhanceable by Nrx-1²⁷³/Nrx-1²⁷³

(Banerjee et al., 2017)

Mad^k00237/Mad¹² has neuromuscular junction | third instar larval stage phenotype, non-enhanceable by Nlg1^Δ46/Nlg1^Δ46

(Banerjee et al., 2017)

Mad^k00237/Mad¹² has terminal bouton | third instar larval stage phenotype, non-enhanceable by Nlg1^Δ46/Nlg1^Δ46

(Banerjee et al., 2017)

Mad^k00237 has embryonic/larval neuromuscular junction | third instar larval stage phenotype, non-enhanceable by trio^S137203

(Ball et al., 2010)

Mad^k00237 has NMJ bouton | third instar larval stage phenotype, non-enhanceable by trio^S137203

(Ball et al., 2010)

Suppressed by

Statement

Reference

Mad^k00237/Mad¹ has embryonic/larval neuromuscular junction | third instar larval stage phenotype, suppressible | partially by Scer\GAL4^BG380/trio^UAS.cBa

(Ball et al., 2010)

Mad^k00237/Mad¹ has NMJ bouton | third instar larval stage phenotype, suppressible | partially by Scer\GAL4^BG380/trio^UAS.cBa

(Ball et al., 2010)

NOT suppressed by

Statement

Reference

Mad^k00237/Mad¹ has embryonic/larval neuromuscular junction | third instar larval stage phenotype, non-suppressible by Scer\GAL4^BG380/Rac1^UAS.Tag:MYC

(Ball et al., 2010)

Mad^k00237/Mad¹ has NMJ bouton | third instar larval stage phenotype, non-suppressible by Scer\GAL4^BG380/Rac1^UAS.Tag:MYC

(Ball et al., 2010)

Suppressor of

Statement

Reference

Mad^k00237/Mad[+] is a suppressor of embryonic/larval neuromuscular junction phenotype of S6KL¹⁴⁰

(Zhao et al., 2015)

Mad^k00237/Mad[+] is a suppressor of NMJ bouton | increased number phenotype of S6KL¹⁴⁰

(Zhao et al., 2015)

Mad^k00237/Mad¹ is a suppressor of embryonic/larval neuromuscular junction | third instar larval stage phenotype of Rac1^UAS.Tag:MYC, Scer\GAL4^BG380

(Ball et al., 2010)

Mad^k00237/Mad¹ is a suppressor of NMJ bouton | third instar larval stage phenotype of Rac1^UAS.Tag:MYC, Scer\GAL4^BG380

(Ball et al., 2010)

Mad^k00237 is a suppressor of eye phenotype of upd1^GMR.PB

(Bach et al., 2003)

Mad^k00237 is a suppressor of embryonic/first instar larval cuticle | maternal effect phenotype of tor^12D

(Li and Li, 2003)

NOT Suppressor of

Statement

Reference

Mad^k00237/Mad¹ is a non-suppressor of embryonic/larval neuromuscular junction | third instar larval stage phenotype of Rac1^V12.UAS, Scer\GAL4^BG380

(Ball et al., 2010)

Mad^k00237/Mad¹ is a non-suppressor of NMJ bouton | third instar larval stage phenotype of Rac1^V12.UAS, Scer\GAL4^BG380

(Ball et al., 2010)

Other

Statement

Reference

Mad^k00237, Rab11^93Bi has NMJ bouton phenotype

(Liu et al., 2014)

Mad^k00237, Rab11^93Bi has neuromuscular junction phenotype

(Liu et al., 2014)

+/Df(3L)Exel6279, Mad^k00237 has NMJ bouton | third instar larval stage phenotype

(Merino et al., 2009)

Mad^k00237/Mad[+], nmo^P1 has NMJ bouton | third instar larval stage phenotype

(Merino et al., 2009)

Additional Comments

Genetic Interactions

Statement

Reference

The decreased number of synaptic boutons at the third instar larval neuromuscular of Mad¹²/Mad^k00237 transheterozygotes is not suppressed or enhanced by either Nrx-1²⁷³ or Nlg1^Δ46 homozygosity.

(Banerjee et al., 2017)

One copy of Mad^k00237 suppresses the increase in total bouton number seen in S6KL¹⁴⁰ mutants.

(Zhao et al., 2015)

Rab11^93Bi, Mad^k00237 double mutants exhibit undergrown synapses with few boutons on neuromuscular junction 4 that are indistinguishable from Mad^k00237 single mutants.

(Liu et al., 2014)

Expression of Mad¹/Mad^k00237 suppresses the larval neuromuscular junction overgrowth and bouton number phenotypes seen when Rac1^{Scer\UAS.T:Hsap\MYC} is expressed in motor neurons under the control of Scer\GAL4^BG380, instead producing a reduction in bouton number similar to the phenotype seen in Mad¹/Mad^k00237 third instar larvae.

Expression of Mad¹/Mad^k00237 fails to suppress the larval neuromuscular junction overgrowth and bouton number phenotypes seen when Rac1^V12.Scer\UAS is expressed in motor neurons under the control of Scer\GAL4^BG380.

trio^S137203 does not enhance the reduction in bouton number phenotype seen in Mad^k00237 larval neuromuscular junctions.

Expression of trio^Scer\UAS.cBa in motor neurons under the control of Scer\GAL4^BG380 partially suppresses the reduced bouton number phenotype seen in Mad¹/Mad^k00237 larval neuromuscular junctions.

(Ball et al., 2010)

Mad^k00237/+ ; nmo^P1/+ and Mad^k00237/+ ; Df(3L)Exel6279/+ double heterozygous third instar larvae show a significant reduction in the number of boutons per muscle surface area at the neuromuscular junction, in contrast to any of the single heterozygotes.

(Merino et al., 2009)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Fails to complement

Mad^unspecified

(Dworkin and Gibson, 2006)

Mad^k05807a

(BDGP Project Members, 1994-1999)

Mad^k00223

(BDGP Project Members, 1994-1999)

Rescued by

Mad^k00237 is rescued by Scer\GAL4^unspecified/Mad^UAS.Tag:MYC

(Merino et al., 2009)

Mad^k00237 is rescued by Scer\GAL4^BG380/Mad^UAS.Tag:MYC

(Merino et al., 2009)

Mad^k00237 is rescued by Scer\GAL4^BG380/Mad^{S25A.UAS.Tag:MYC}

(Merino et al., 2009)

Partially rescued by

Mad^k00237 is partially rescued by Mad^hs.PK

(Kaneko et al., 2011)

Mad^k00237 is partially rescued by Mad^T312V.hs

(Kaneko et al., 2011)

Mad^k00237 is partially rescued by Scer\GAL4^unspecified/Mad^{S25A.UAS.Tag:MYC}

(Merino et al., 2009)

Comments

Expression of Mad^hs.PK using heat shock partially rescues the lethality of Mad^k00237 mutants, with the rescue efficiency being increased with more frequent heat shocks.

Expression of Mad^T312V.hs using heat shock partially rescues the lethality of Mad^k00237 mutants, with the rescue efficiency being increased with more frequent heat shocks. The degree of rescue with Mad^T312V.hs is more effective than that seen with Mad^hs.PK under the same conditions.

(Kaneko et al., 2011)

Neuronal expression of Mad^{Scer\UAS.T:Hsap\MYC} under the control of Scer\GAL4^unspecified rescues the reduction in the number of boutons per muscle surface area that is seen at the neuromuscular junction in Mad^k00237 third instar larvae.

Neuronal expression of Mad^{S25A.Scer\UAS.T:Hsap\MYC} under the control of Scer\GAL4^unspecified partially rescues the reduction in the number of boutons per muscle surface area that is seen at the neuromuscular junction in Mad^k00237 third instar larvae.

Expression of either Mad^{Scer\UAS.T:Hsap\MYC} or Mad^{S25A.Scer\UAS.T:Hsap\MYC} under the control of Scer\GAL4^BG380 rescues the electrophysiological defects seen at the neuromuscular junction of Mad^k00237 third instar larvae.

(Merino et al., 2009)

Images (0)

Mutant

Wild-type

Stocks (2)

Bloomington

10474

y¹ w^67c23; P{lacW}Mad^k00237/CyO

Kyoto

111123

y^d2 w¹¹¹⁸ P{ey-FLP.N}2 P{5xglBS-lacZ.38-1}TPN1; P{lacW}Mad^k00237 P{neoFRT}40A/CyO y⁺

Notes on Origin

Discoverer

I. Kiss.

(BDGP Project Members, 1994-1999)

Comments

Complements: toc⁰¹³⁶¹. Complements: l(2)k05816^k05816.

(BDGP Project Members, 1994-1999)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (9)

Reported As

Symbol Synonym

Mad²³⁷

(Banerjee et al., 2017, Zhao et al., 2015, Ball et al., 2010, Merino et al., 2009)

Mad^K00237

(Zhou et al., 2023, Lin et al., 2021, Liao et al., 2018, Merino et al., 2009)

Mad^K237

(Galindo et al., 2002, Galindo et al., 2002)

Mad^k00237

(Sharma et al., 2022, Ho and Treisman, 2020, Li et al., 2020, Huang et al., 2011, Ball et al., 2010)

k00237

(Dworkin et al., 2011, Dworkin and Gibson, 2006)

l(2)k00237

(Spradling et al., 1999)

l(2)k00237^k00237

mad^K00237

(Liu et al., 2014, Dworkin and Gibson., 2006)

mad^k00237

(Kaneko et al., 2011)

Name Synonyms

Secondary FlyBase IDs

References (25)

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