FlyBase Allele Report: Dmel\Rab11[93Bi]

Embryo hatching rate is somewhat reduced in Rab11^93Bi/Rab11^93Bi mutants compared to wild type. Embryo hatching rate is massively reduced in Rab11^93Bi/Rab11^j2D1 mutants (around 10% hatch) compared to wild type.

(Zheng et al., 2016)

Rab11^93Bi mutants exhibit synaptic overgrowth with excess satellite boutons on neuromuscular junction 4.

(Liu et al., 2014)

Homozygotes show a small increase in the total number of multi-nucleate spermatids compared to controls.

(Robinett et al., 2009)

Testes from Rab11^93Bi homozygous larvae, or larvae in which Rab11^93Bi is transheterozygous with any of Rab11^EP3017, Rab11^ETo11 or Rab11^ETo3, have spermatids composed of an abnormally large Nebenkern associated with multiple nuclei.

Mid and late telophases in Rab11^93Bi/Rab11^ETo11 spermatocytes display contractile rings that are not fully constricted or poorly constricted and broken into irregular pieces. Defective central spindles are seen in 80% of these cases. In those spermatocytes that failed to complete cytokinesis, the furrow ingresses to a limited extent and then regresses, coincident with disassembly of the central spindle.

48% of telophases in Rab11^93Bi/Rab11^ETo11 spermatocytes display abnormal localization of Golgi-derived vesicle-like structures.

Rab11^93Bi/Rab11^ETo11 testes show disorganized acroblasts in both early and (particularly) elongating spermatids.

Rab11^93Bi/Rab11^ETo11 brains do not exhibit polyploid mitoses.

(Giansanti et al., 2007)

Rab11^93Bi/+ flies show no eye phenotype.

Rab11^ex1/Rab11^93Bi transheterozygotes are mostly lethal but a few survive to adulthood.

The posterior abdomens of Rab11^ex1/Rab11^93Bi flies have empty sockets that are missing microchaetae. Additionally, posterior scutellar bristles are shortened in these mutants. Rab11^93Bi homozygotes also show microchaetae loss, although this is less extensive.

The synapse of muscle 6/7 shows an increase in boutons by about 60% in Rab11^ex1/Rab11^93Bi mutants compared to controls. The boutons of Rab11^ex1/Rab11^93Bi mutants are often clustered resulting in a 'bunch of grapes' morphology instead of the wild-type 'beads-on-a-string' morphology. The aberrant morphology may reflect increased branching (by 2.6 fold) of Rab11^ex1/Rab11^93Bi boutons.

(Khodosh et al., 2006)

8% of Rab11^93Bi/Rab11^j2D1-derived embryos exhibit disrupted nuclear morphology in premigration and early cortical divisions. During the late cortical divisions, when the nuclei are more densely packed, 65% of embryos exhibit severely disrupted nuclear morphology. In Rab11^93Bi/Rab11^j2D1-derived nuclear cycle 12 embryos, actin cap formation occurs normally. As the embryos progress into prophase, the actin caps are dismantled as the actin re-organises into furrows encompassing each prophase nucleus and its developing spindle. In Rab11^93Bi/Rab11^j2D1-derived embryos, the hexagonal furrow network is riddled with gaps, present at prophase during the initial stages of furrow formation. Similar defects are observed during cellularisation at nuclear cycle 14.

(Riggs et al., 2003)

Homozygotes have weak bristle defects. 7.7% of eggs laid by Rab11^93Bi/Rab11^j2D1 females develop into larvae. 2% of the escapers have a germ cell-less phenotype.

(Jankovics et al., 2001)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Rab11^93Bi has some die during embryonic stage phenotype, enhanceable by Nin¹

(Zheng et al., 2016)

Rab11^93Bi/Rab11^j2D1 has majority die during embryonic stage phenotype, enhanceable by Nin¹

(Zheng et al., 2016)

Rab11^93Bi has abnormal meiotic cell cycle | male phenotype, enhanceable by bru[+]/brun^Z3358

(Robinett et al., 2009)

Rab11^93Bi has abnormal cytokinesis | male phenotype, enhanceable by bru[+]/brun^Z3358

(Robinett et al., 2009)

Suppressed by

Statement

Reference

Rab11^93Bi/Rab11^ex1 has lethal phenotype, suppressible | partially by Df(2L)cl7/+

(Khodosh et al., 2006)

Rab11^93Bi/Rab11^ex1 has lethal phenotype, suppressible | partially by Df(2L)cl7/bchs¹⁷

(Khodosh et al., 2006)

Rab11^93Bi/Rab11^ex1 has lethal phenotype, suppressible | partially by bchs¹⁷/bchs¹²

(Khodosh et al., 2006)

Rab11^93Bi/Rab11^ex1 has lethal phenotype, suppressible | partially by bchs¹⁷/bchs[+]

(Khodosh et al., 2006)

Rab11^93Bi/Rab11^ex1 has abnormal neuroanatomy phenotype, suppressible | partially by bchs¹⁷/bchs¹²

(Khodosh et al., 2006)

Enhancer of

Statement

Reference

Rab11^93Bi/Rab11[+] is an enhancer of abnormal neuroanatomy | larval stage phenotype of Acsl⁰⁵⁸⁴⁷/Acsl⁸

(Liu et al., 2014)

Rab11^93Bi/Rab11^93Bi is an enhancer of abnormal neuroanatomy | larval stage phenotype of Acsl⁰⁵⁸⁴⁷/Acsl⁸

(Liu et al., 2014)

Rab11^93Bi/Rab11[+] is an enhancer of visible phenotype of Scer\GAL4^GMR.PF, bchs^EP2299

(Simonsen et al., 2007)

NOT Enhancer of

Statement

Reference

Rab11^93Bi/Rab11^ETo11 is a non-enhancer of abnormal cytokinesis | male phenotype of fwd^Z0453/fwd³

(Giansanti et al., 2007)

Rab11^93Bi/Rab11^ETo11 is a non-enhancer of abnormal cytokinesis | male phenotype of vib^EP513

(Giansanti et al., 2007)

NOT Suppressor of

Statement

Reference

Rab11^93Bi/Rab11^ETo11 is a non-suppressor of abnormal cytokinesis | male phenotype of vib^EP513

(Giansanti et al., 2007)

Rab11^93Bi/Rab11^ETo11 is a non-suppressor of abnormal cytokinesis | male phenotype of fwd^Z0453/fwd³

(Giansanti et al., 2007)

Other

Statement

Reference

Mad^k00237, Rab11^93Bi has abnormal neuroanatomy | larval stage phenotype

(Liu et al., 2014)

Acsl⁰⁵⁸⁴⁷/Acsl^KO, Rab11^93Bi/Rab11[+] has lethal - all die before end of larval stage phenotype

(Liu et al., 2014)

Rab11^93Bi, brun^Z3358 has lethal | larval stage phenotype

(Robinett et al., 2009)

Rab11^93Bi/Rab11^ETo3, brun^Z3358 has lethal | larval stage phenotype

(Robinett et al., 2009)

Rab11^93Bi/Rab11^ETo11, fwd^Z0453/fwd³ has abnormal cytokinesis | male phenotype

(Giansanti et al., 2007)

Rab11^93Bi/Rab11^ETo11, vib^EP513 has abnormal cytokinesis | male phenotype

(Giansanti et al., 2007)

Phenotype Manifest In

Enhanced by

Statement

Reference

Rab11^93Bi has spermatid phenotype, enhanceable by bru[+]/brun^Z3358

(Robinett et al., 2009)

Suppressed by

Statement

Reference

Rab11^93Bi/Rab11^ex1 has posterior scutellar bristle phenotype, suppressible | partially by Df(2L)cl7/bchs¹⁷

(Khodosh et al., 2006)

Rab11^93Bi/Rab11^ex1 has posterior scutellar bristle phenotype, suppressible | partially by bchs¹⁷/bchs¹²

(Khodosh et al., 2006)

Rab11^93Bi has microchaeta phenotype, suppressible by bchs⁵⁸/bchs⁵⁸

(Khodosh et al., 2006)

Rab11^93Bi has microchaeta phenotype, suppressible | partially by bchs⁵⁸/bchs[+]

(Khodosh et al., 2006)

Rab11^93Bi has microchaeta phenotype, suppressible | partially by bchs^8K/bchs[+]

(Khodosh et al., 2006)

Rab11^93Bi/Rab11^ex1 has bouton phenotype, suppressible | partially by bchs¹⁷/bchs¹²

(Khodosh et al., 2006)

Rab11^93Bi/Rab11^ex1 has microchaeta phenotype, suppressible | partially by bchs¹⁷/bchs[+]

(Khodosh et al., 2006)

Rab11^93Bi/Rab11^ex1 has microchaeta phenotype, suppressible | partially by Df(2L)cl7/+

(Khodosh et al., 2006)

Rab11^93Bi/Rab11^ex1 has microchaeta phenotype, suppressible by Df(2L)cl7/bchs¹⁷

(Khodosh et al., 2006)

Rab11^93Bi/Rab11^ex1 has microchaeta phenotype, suppressible by bchs¹⁷/bchs¹²

(Khodosh et al., 2006)

Rab11^93Bi/Rab11^ex1 has posterior scutellar bristle phenotype, suppressible | partially by bchs¹⁷/bchs[+]

(Khodosh et al., 2006)

Rab11^93Bi/Rab11^ex1 has posterior scutellar bristle phenotype, suppressible | partially by Df(2L)cl7/+

(Khodosh et al., 2006)

Enhancer of

Statement

Reference

Rab11^93Bi/Rab11[+] is an enhancer of neuromuscular junction phenotype of Acsl⁰⁵⁸⁴⁷/Acsl⁸

(Liu et al., 2014)

Rab11^93Bi/Rab11^93Bi is an enhancer of NMJ bouton | increased number phenotype of Acsl⁰⁵⁸⁴⁷/Acsl⁸

(Liu et al., 2014)

Rab11^93Bi/Rab11[+] is an enhancer of eye phenotype of Scer\GAL4^GMR.PF, bchs^EP2299

(Simonsen et al., 2007, Khodosh et al., 2006)

NOT Enhancer of

Statement

Reference

Rab11^93Bi/Rab11^ETo11 is a non-enhancer of spermatid phenotype of fwd^Z0453/fwd³

(Giansanti et al., 2007)

Rab11^93Bi/Rab11^ETo11 is a non-enhancer of spermatid phenotype of vib^EP513

(Giansanti et al., 2007)

NOT Suppressor of

Statement

Reference

Rab11^93Bi/Rab11^ETo11 is a non-suppressor of spermatid phenotype of vib^EP513

(Giansanti et al., 2007)

Rab11^93Bi/Rab11^ETo11 is a non-suppressor of spermatid phenotype of fwd^Z0453/fwd³

(Giansanti et al., 2007)

Other

Statement

Reference

Mad^k00237, Rab11^93Bi has NMJ bouton phenotype

(Liu et al., 2014)

Mad^k00237, Rab11^93Bi has neuromuscular junction phenotype

(Liu et al., 2014)

Rab11^93Bi/Rab11^ETo11, fwd^Z0453/fwd³ has spermatid phenotype

(Giansanti et al., 2007)

Rab11^93Bi/Rab11^ETo11, vib^EP513 has spermatid phenotype

(Giansanti et al., 2007)

Additional Comments

Genetic Interactions

Statement

Reference

Bsg25D¹ leads to a small but significant enhancements of reduced embryo hatching rates in Rab11^93Bi/Rab11^93Bi or Rab11^93Bi/Rab11^j2D1 mutants.

(Zheng et al., 2016)

Rab11^93Bi, Mad^k00237 double mutants exhibit undergrown synapses with few boutons on neuromuscular junction 4 that are indistinguishable from Mad^k00237 single mutants.

Acsl⁰⁵⁸⁴⁷/Acsl^KO Rab11^93Bi/+ animals do not survive to wandering third instar larvae.

Rab11^93Bi/+ and, to a greater extent, Rab11^93Bi/Rab11^93Bi significantly increases neuromuscular junction overgrowth of Acsl⁰⁵⁸⁴⁷/Acsl⁸ mutants.

(Liu et al., 2014)

Flies homozygous for both bru^Z3358 and Rab11^93Bi die in early larval stages at 25[o]C, as do flies homozygous for bru^Z3358 and transheterozygous for Rab11^93Bi/Rab11^ETo3.

bru^Z3358/+ males also homozygous for Rab11^93Bi display a four-fold increase in the total number of multi-nucleate spermatids compared with males homozygous for Rab11^93Bi alone.

(Robinett et al., 2009)

Rab11^93Bi/Rab11^ETo11, fwd³/fwd^Z0453 spermatids are multinucleated at a similar frequency to fwd³/fwd^Z0453 spermatids.

Rab11^93Bi/Rab11^ETo11, vib^EP513 spermatids are multinucleated at a similar frequency to vib^EP513 spermatids.

(Giansanti et al., 2007)

Rab11^93Bi heterozygotes act as dominant enhancers of the Scer\GAL4^GMR.PF>bchs^EP2299 eye phenotype, resulting in adults with severely reduced eyes.

The viability of Rab11^ex1/Rab11^93Bi is increased when flies are also mutant for either Df(2L)cl7/+, Df(2L)cl7/bchs¹⁷, bchs¹⁷/bchs¹² or bchs¹⁷/+.

The microchaeta loss of Rab11^ex1/Rab11^93Bi flies is significantly suppressed by Df(2L)cl7/+ or bchs¹⁷/+ and is completely suppressed by Df(2L)cl7/bchs¹⁷ or bchs¹⁷/bchs¹². Further, posterior scutellar bristle length of Rab11^ex1/Rab11^93Bi flies is restored partially by Df(2L)cl7/+ or bchs¹⁷/+ and more fully by Df(2L)cl7/bchs¹⁷ or bchs¹⁷/bchs¹².

The microchaeta loss of Rab11^93Bi flies is significantly suppressed by bchs⁵⁸/+ or bchs^8K/+ and is completely suppressed by bchs⁵⁸/bchs⁵⁸.

The bouton density and branching defects of Rab11^ex1/Rab11^93Bi larval NMJs at muscle 6/7 are significantly suppressed in bchs¹⁷/bchs¹²; Rab11^ex1/Rab11^93Bi double mutants.

(Khodosh et al., 2006)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Fails to complement

Rab11^ETo11

(Jankovics et al., 2001)

Rab11^ETo3

(Jankovics et al., 2001)

Rab11^EP3017

(Jankovics et al., 2001)

Rab11^j2D1

(Jankovics et al., 2001)

Rescued by

Rab11^93Bi/Rab11^ETo3 is rescued by Rab11^GFP

(Giansanti et al., 2007)

Rab11^93Bi/Rab11^ETo11 is rescued by Rab11^GFP

(Giansanti et al., 2007)

Comments

Rab11^93Bi/Rab11^ETo3 or Rab11^93Bi/Rab11^ETo11 flies carrying Rab11^T:Avic\GFP are fully viable and testes from larvae of these genotypes do not exhibit abnormal spermatids.

(Giansanti et al., 2007)

Images (0)

Mutant

Wild-type

Stocks (2)

Bloomington

4158

Rab11^93Bi e^LE1 cd¹/TM3, Sb¹ Ser¹

Kyoto

107602

Rab11^93Bi e^LE1 cd¹/TM3, Sb¹ Ser¹

Notes on Origin

Discoverer

T. Vincent

(Rawls, 1998.7.3)

Comments

Order of alleles (based on transheterozygous sterile phenotype): Rab11^93Bi < Rab11^j2D1 < Rab11^EP3017 = Rab11^ETo11 = Rab11^ETo3 < Df(3R)e-N19.

(Jankovics et al., 2001)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (5)

Reported As

Symbol Synonym

Rab11^93Bi

(Zheng et al., 2016, Giansanti et al., 2015, Belloni et al., 2012, Robinett et al., 2009)

l(3)93Bi¹

(Eisenberg et al., 1990)

rab11^93Bi

(Walsh et al., 2021, Huang et al., 2018, Huang et al., 2016, Liu et al., 2014, Giansanti et al., 2007, Simonsen et al., 2007, Khodosh et al., 2006)

rab11[93Bi]

(Guichard et al., 2013)

rlr7

(Rawls, 1998.7.3)

Name Synonyms

Secondary FlyBase IDs

References (17)

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