FlyBase Allele Report: Dmel\Nlg1[Δ46]

General Information

Symbol

Dmel\Nlg1^Δ46

Species

D. melanogaster

Name

FlyBase ID

FBal0279828

Feature type

allele

Associated gene

Dmel\Nlg1

Associated Insertion(s)

Carried in Construct

Also Known As

dnlg1^Δ46

Key Links

Allele class

Mutagen

FLPase

Nature of the Allele

Allele class

Mutagen

FLPase

(Mozer and Sandstrom, 2012)

Progenitor genotype

PBac{WH}Nlg1^f00713

(Mozer and Sandstrom, 2012)

PBac{WH}f00735

(Mozer and Sandstrom, 2012)

Cytology

Description

FLP-mediated recombination between the FRT sites of PBac{WH}f00735 and PBac{WH}Nlg1^f00713 generates an approximately 29kb deletion within Nlg1 that removes the transcription start site and most of the extracellular domain of the protein.

(Mozer and Sandstrom, 2012)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neuroanatomy

(Mozer and Sandstrom, 2012)

abnormal neuroanatomy (with Df(3R)ED5223)

(Mozer and Sandstrom, 2012)

abnormal neurophysiology

(Mozer and Sandstrom, 2012)

abnormal neurophysiology (with Df(3R)ED5223)

(Mozer and Sandstrom, 2012)

viable

(Banerjee et al., 2017)

Phenotype Manifest In

Detailed Description

Statement

Reference

Nlg1^Δ46 homozygotes are viable. Nlg1^Δ46 leads to a significant changes in the third instar larval neuromuscular junction compared to controls: a decrease in the number, but not in the area, of synaptic boutons; a significant increase in the active zone density; a significant increase in the total postsynaptic density length; a significant increase in the ruffle density at the synapse; and significant decreases in the subsynaptic reticulum length and density.

(Banerjee et al., 2017)

Nlg1^Δ46 mutants exhibit smaller, weaker synapses than in wild-type.

Nlg1^Δ46/Df(3R)ED5223 mutants display a reduction in synaptic boutons compared to controls.

Nlg1^Δ46 homozygotes and Nlg1^Δ46/Df(3R)ED5223 transheterozygotes exhibit a significant reduction in the amplitude of EJCs evoked by nerve shock, compared to wild-type. Amplitude distributions of miniature EJCs (mEJCs) are abnormal in Nlg1^Δ46 mutants, displaying a significantly broader distribution, compared to wild-type. Despite this change in distribution, the mean amplitude of mEJCs remains as in wild-type. Therefore, the primary affect of Nlg1^Δ46 is to reduce the quantal content, the number of vesicles released by action potential, by more than 60%.

Ca[2+] sensitivity of vesicle fusion is unaltered in Nlg1^Δ46 mutants. The primary difference between mutant and wild-type is the size of synaptic current at maximal release.

(Mozer and Sandstrom, 2012)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Enhanced by

Statement

Reference

Nlg1^Δ46 has neuromuscular junction | third instar larval stage phenotype, enhanceable by wit^B11/wit^A12

(Banerjee et al., 2017)

Nlg1^Δ46 has terminal bouton | third instar larval stage phenotype, enhanceable by wit^B11/wit^A12

(Banerjee et al., 2017)

NOT Enhanced by

Statement

Reference

Nlg1^Δ46 has neuromuscular junction | third instar larval stage phenotype, non-enhanceable by Nrx-1²⁷³/Nrx-1²⁷³

(Banerjee et al., 2017)

Nlg1^Δ46 has terminal bouton | third instar larval stage phenotype, non-enhanceable by Nrx-1²⁷³/Nrx-1²⁷³

(Banerjee et al., 2017)

Nlg1^Δ46 has presynaptic active zone | third instar larval stage phenotype, non-enhanceable by Nrx-1²⁷³/Nrx-1²⁷³

(Banerjee et al., 2017)

Nlg1^Δ46 has postsynaptic density | third instar larval stage phenotype, non-enhanceable by Nrx-1²⁷³/Nrx-1²⁷³

(Banerjee et al., 2017)

Nlg1^Δ46 has subsynaptic reticulum | third instar larval stage phenotype, non-enhanceable by Nrx-1²⁷³/Nrx-1²⁷³

(Banerjee et al., 2017)

NOT Enhancer of

Statement

Reference

Nlg1^Δ46/Nlg1^Δ46 is a non-enhancer of neuromuscular junction | third instar larval stage phenotype of Nrx-1²⁷³

(Banerjee et al., 2017)

Nlg1^Δ46/Nlg1^Δ46 is a non-enhancer of terminal bouton | third instar larval stage phenotype of Nrx-1²⁷³

(Banerjee et al., 2017)

Nlg1^Δ46/Nlg1^Δ46 is a non-enhancer of neuromuscular junction | third instar larval stage phenotype of wit^B11/wit^A12

(Banerjee et al., 2017)

Nlg1^Δ46/Nlg1^Δ46 is a non-enhancer of terminal bouton | third instar larval stage phenotype of wit^B11/wit^A12

(Banerjee et al., 2017)

Nlg1^Δ46/Nlg1^Δ46 is a non-enhancer of presynaptic active zone | third instar larval stage phenotype of wit^B11/wit^A12

(Banerjee et al., 2017)

Nlg1^Δ46/Nlg1^Δ46 is a non-enhancer of presynaptic active zone | third instar larval stage phenotype of Nrx-1²⁷³

(Banerjee et al., 2017)

Nlg1^Δ46/Nlg1^Δ46 is a non-enhancer of postsynaptic density | third instar larval stage phenotype of wit^B11/wit^A12

(Banerjee et al., 2017)

Nlg1^Δ46/Nlg1^Δ46 is a non-enhancer of subsynaptic reticulum | third instar larval stage phenotype of wit^B11/wit^A12

(Banerjee et al., 2017)

Nlg1^Δ46/Nlg1^Δ46 is a non-enhancer of neuromuscular junction | third instar larval stage phenotype of Mad^k00237/Mad¹²

(Banerjee et al., 2017)

Nlg1^Δ46/Nlg1^Δ46 is a non-enhancer of terminal bouton | third instar larval stage phenotype of Mad^k00237/Mad¹²

(Banerjee et al., 2017)

Nlg1^Δ46/Nlg1^Δ46 is a non-enhancer of postsynaptic density | third instar larval stage phenotype of Nrx-1²⁷³

(Banerjee et al., 2017)

Nlg1^Δ46/Nlg1^Δ46 is a non-enhancer of subsynaptic reticulum | third instar larval stage phenotype of Nrx-1²⁷³

(Banerjee et al., 2017)

Other

Statement

Reference

Nlg1[+]/Nlg1^Δ46, Nrx-1²⁷³ has neuromuscular junction | third instar larval stage phenotype

(Banerjee et al., 2017)

Additional Comments

Genetic Interactions

Statement

Reference

Nlg1^Δ46, Nrx-1²⁷³ double homozygotes show third instar larval neuromuscular junction defects that are similar to those observed in either single mutant, although there are ocasional giant boutons that are laterally elongated with multiple active zones and reduced subsynaptic reticulum; Nrx-1²⁷³, Nlg1^Δ46 double heterozygotes also present a significant decrease in the number of synaptic boutons at the third instar larval neuromuscular junction, as compared to single heterozygous and wild-type controls.

Nlg1^Δ46 homozygosity does not suppress or enhance the third instar larval neuromuscular junction defects displayed by wit^B11/wit^A12 transheterozygotes. Nlg1^Δ46 homozygosity also does not suppress or enhance the decreased number of synaptic boutons at the third instar larval neuromuscular of Mad¹²/Mad^k00237 transheterozygotes.

(Banerjee et al., 2017)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

Nlg1^Δ46 is rescued by Nlg1^+tMa

(Mozer and Sandstrom, 2012)

Nlg1^Δ46/Df(3R)ED5223 is rescued by Nlg1^+tMa

(Mozer and Sandstrom, 2012)

Partially rescued by

Nlg1^Δ46 is partially rescued by Scer\GAL4^Mef2.PU/Nlg1^UAS.cMa

(Banerjee et al., 2017)

Nlg1^Δ46 is partially rescued by Scer\GAL4^Mef2.PU/Nlg1^{Δextra.UAS.Tag:MYC,EGFP}

(Banerjee et al., 2017)

Not rescued by

Nlg1^Δ46 is not rescued by Nlg1^{Δcyto.UAS.EGFP}/Scer\GAL4^Mef2.PU

(Banerjee et al., 2017)

Comments

The decreased number of synaptic boutons observed at the Nlg1^Δ46 homozygous third instar larval neuromuscular junction is partially rescued by the expression of either Nlg1^Scer\UAS.cMa or Nlg1^{Δextra.Scer\UAS.T:Hsap\MYC,T:Avic\GFP-EGFP}, but not of Nlg1^{Δcyto.Scer\UAS.T:Avic\GFP-EGFP}, under the control of Scer\GAL4^elav.PU.

(Banerjee et al., 2017)

Images (0)

Mutant

Wild-type

Stocks (2)

Bloomington

78859

w^*; Nlg1^Δ46/TM6B, Tb¹

78860

w^*; PBac{Nlg1^+tMa}VK00037; Nlg1^Δ46/TM6B, Tb¹

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

Nlg1^Δ46

dnlg1^Δ46

(Banerjee et al., 2017, Mozer and Sandstrom, 2012)

nlg1^Δ46

(Sulkowski et al., 2016)

Name Synonyms

Secondary FlyBase IDs

References (3)

Report Sections

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