FB2024_03 , released June 25, 2024
Allele: Dmel\Nlg1Δextra.UAS.Tag:MYC,EGFP
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General Information
Symbol
Dmel\Nlg1Δextra.UAS.Tag:MYC,EGFP
Species
D. melanogaster
Name
FlyBase ID
FBal0246580
Feature type
allele
Associated gene
Dmel\Nlg1
Associated Insertion(s)
Carried in Construct
M{UAS-Nlg1.Δextra.EGFP}
Key Links
Transgenic product class
inframe_deletion
(Banovic et al., 2010)
Mutagen
Nature of the Allele
Transgenic product class
inframe_deletion
(Banovic et al., 2010)
Mutagen
in vitro construct
(Banovic et al., 2010)
Progenitor genotype
Carried in construct
M{UAS-Nlg1.Δextra.EGFP}
Cytology
Description

UAS regulatory sequences drive expression of Nlg1 in which amino acids 1-741 have been deleted and replaced by a signal peptide from Rnor\Cd2 followed by 10 Tag:MYC tags. In addition, the Nlg1 sequence is tagged with EGFP (the tag is just C-terminal to the transmembrane domain).

(Banovic et al., 2010)
Allele components
Component
Use(s)
Regulatory region(s)
UAS
Encoded product / tool
Nlg1
Tagged with
EGFP
Tag:MYC
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      The expression of Nlg1Δextra.Scer\UAS.T:Hsap\MYC,T:Avic\GFP-EGFP under the control of Scer\GAL4Mef2.PU does not lead to significant changes in the number of synaptic boutons at the third instar larval neuromuscular junction, as compared to controls.

      (Banerjee et al., 2017)

      Expression of Nlg1Δextra.Scer\UAS.T:Hsap\MYC,T:Avic\GFP-EGFP under the control of Scer\GAL4Mef2.PR has no effect on the morphology of the neuromuscular junction.

      (Banovic et al., 2010)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Partially rescues

      Scer\GAL4Mef2.PU/Nlg1Δextra.UAS.Tag:MYC,EGFP partially rescues Nlg1Δ46

      (Banerjee et al., 2017)
      Fails to rescue

      Scer\GAL4Mef2.PR/Nlg1Δextra.UAS.Tag:MYC,EGFP fails to rescue Nlg1I960/Nlg1H324

      (Banovic et al., 2010)
      Comments

      The expression of Nlg1Δextra.Scer\UAS.T:Hsap\MYC,T:Avic\GFP-EGFP under the control of Scer\GAL4Mef2.PU partially rescues the decreased number of synaptic boutons observed at the Nlg1Δ46 homozygous third instar larval neuromuscular junction.

      (Banerjee et al., 2017)

      Expression of Nlg1Δextra.Scer\UAS.T:Hsap\MYC,T:Avic\GFP-EGFP under the control of Scer\GAL4Mef2.PR does not rescue the neuromuscular junction defects of Nlg1I960/Nlg1H324 larvae.

      (Banovic et al., 2010)
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      Nlg1Δextra.Scer\UAS.T:Hsap\MYC,T:Avic\GFP-EGFP
      Nlg1Δextra.UAS.Tag:MYC,EGFP
      Name Synonyms
      Secondary FlyBase IDs
        References (2)