FB2024_03 , released June 25, 2024
Allele: Dmel\Nlg1UAS.cMa
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General Information
Symbol
Dmel\Nlg1UAS.cMa
Species
D. melanogaster
Name
FlyBase ID
FBal0279830
Feature type
allele
Associated gene
Dmel\Nlg1
Associated Insertion(s)
Carried in Construct
P{UAS-Nlg1.M}
Also Known As
UAS-dnlg1
Key Links
Transgenic product class
cDNA
(Mozer and Sandstrom, 2012)
wild_type
(Mozer and Sandstrom, 2012)
Mutagen
Nature of the Allele
Transgenic product class
cDNA
(Mozer and Sandstrom, 2012)
wild_type
(Mozer and Sandstrom, 2012)
Mutagen
in vitro construct
(Mozer and Sandstrom, 2012)
Progenitor genotype
Carried in construct
P{UAS-Nlg1.M}
Cytology
Description

UASt regulatory sequences drive expression of Nlg1 coding sequences that correspond to cDNA clone RE29404.

(Mozer and Sandstrom, 2012)
Allele components
Component
Use(s)
Regulatory region(s)
UASt
Encoded product / tool
Nlg1
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      The expression of Nlg1Scer\UAS.cMa under the control of Scer\GAL4Mef2.PU does not lead to significant changes in the number of synaptic boutons at the third instar larval neuromuscular junction, as compared to controls.

      (Banerjee et al., 2017)

      Driving expression of wild-type Nlg1Scer\UAS.cMa in the muscle using Scer\GAL4BG487 increases bouton number above wild-type levels by greater than 40%.

      Overexpression of Nlg1Scer\UAS.cMa in the muscles of wild-type larvae (under the control of Scer\GAL4BG487) reduces mEJC amplitude by 25% and EJC amplitude by over 60%.

      Nlg1Scer\UAS.cMa overexpression in muscle (under the control of Scer\GAL4BG487) causes synaptic overgrowth and inhibition of glutamate release.

      (Mozer and Sandstrom, 2012)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Suppressed by
      Statement
      Reference

      Nlg1UAS.cMa, Scer\GAL4BG487 has synapse phenotype, suppressible by Nrx-1273

      (Mozer and Sandstrom, 2012)

      Nlg1UAS.cMa, Scer\GAL4BG487 has bouton phenotype, suppressible by Nrx-1273

      (Mozer and Sandstrom, 2012)
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      Synaptic overgrowth caused by Nlg1Scer\UAS.cMa overexpression in the muscle (under the control of Scer\GAL4BG487) is suppressed by Nrx-1273.

      Overexpression of Nlg1Scer\UAS.cMa in a Nrx-1273 mutant background does not increase bouton number above that of the negative control (Scer\GAL4BG487/Nlg1Scer\UAS.cMa; Nrx-1273/+).

      The inhibitory effects of Nlg1Scer\UAS.cMa overexpression on evoked synaptic transmission are suppressed in Nrx-1273 mutants. EJC amplitude is also significantly restored in these flies.

      (Mozer and Sandstrom, 2012)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Partially rescues

      Scer\GAL4Mef2.PU/Nlg1UAS.cMa partially rescues Nlg1Δ46

      (Banerjee et al., 2017)
      Comments

      The expression of Nlg1Scer\UAS.cMa under the control of Scer\GAL4Mef2.PU rescues the decreased number of synaptic boutons observed at the Nlg1Δ46 homozygous third instar larval neuromuscular junction.

      (Banerjee et al., 2017)

      Driving expression of wild-type Nlg1Scer\UAS.cMa in the muscles of Nlg1Δ46 mutants using Scer\GAL4BG487 increases bouton number above wild-type levels by greater than 40%.

      Unlike bouton number, EJC amplitude in Nlg1Δ46 mutants is not restored by postsynaptic expression of Nlg1Scer\UAS.cMa.

      (Mozer and Sandstrom, 2012)
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      Nlg1Scer\UAS.cMa
      Nlg1UAS.cMa
      Name Synonyms
      Secondary FlyBase IDs
        References (2)