FlyBase Allele Report: Dmel\kst[2]

General Information

Symbol

Dmel\kst²

Species

D. melanogaster

Name

FlyBase ID

FBal0064834

Feature type

allele

Associated gene

Dmel\kst

Associated Insertion(s)

Carried in Construct

Key Links

Allele class

loss of function allele

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

loss of function allele

(Thomas and Kiehart, 1995)

Mutagen

ethyl methanesulfonate

(Thomas et al., 1998)

Progenitor genotype

Cytology

Description

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

Detailed Description

Statement

Reference

Border cells migrate ahead of outer follicle cells in 72% of kst²/kst⁰¹³¹⁸ egg chambers.

(Urwyler et al., 2012)

Most of the kst²/Df(3L)1226 mutant larvae die as larvae.

Most of the kst²/kst^B1-14.1 mutant larvae die as larvae.

(Lee and Thomas, 2011)

Rhabdomeres of kst¹/kst² mutant eyes show defects in rhabdomere shape similar to, but less marked than those of crb mutants. Stalk membranes are 10% shorter than wild type and lack the wild type deep folds.

(Pellikka et al., 2002)

Mutant embryos hatch, but mutant pupae are present at less than 10% of the expected number, suggesting that most individuals die as larvae. There seems to be little pupal lethality and kst mutants eclose 2-4 days behind their wild-type siblings. Adult escapers are very infertile and lay few eggs. kst¹/kst² adult escapers have rough eyes and wings that typically curve downwards in an "inverted spoon" shape. The R7 photoreceptor cell is missing in many ommatidia in kst²/Df(3L)1226 escapers.

(Thomas et al., 1998)

Homozygotes generally die as larvae. Surviving adults have defects in the eyes, wings and tracheal system.

(Thomas and Kiehart, 1995)

Hemizygotes have irregular or missing facets in a region of variable size at the posterior margin of the eye. The wings show a variable degree of downward curvature and the flies tend to "leak" haemolymph, particularly through the spiracles of the abdomen.

(Thomas and Kiehart, 1992)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

kst¹/kst² has lethal phenotype, enhanceable by crb^11A22/crb[+]

(Medina et al., 2002)

kst^B1-14.1/kst² has lethal phenotype, enhanceable by crb^11A22/crb[+]

(Medina et al., 2002)

Suppressed by

Statement

Reference

Df(3L)1226/kst² has lethal | larval stage phenotype, suppressible | partially by Pak[+]/Pak⁶

(Lee and Thomas, 2011)

kst^B1-14.1/kst² has lethal | larval stage phenotype, suppressible | partially by Pak[+]/Pak⁶

(Lee and Thomas, 2011)

Enhancer of

Statement

Reference

kst[+]/kst² is an enhancer of visible phenotype of Scer\GAL4^GMR.PFa, trio^UAS.cBa

(Lee and Thomas, 2011)

NOT Enhancer of

Statement

Reference

kst[+]/kst² is a non-enhancer of lethal | embryonic stage | recessive | partially phenotype of peb³⁰⁸

(Wilk et al., 2004)

NOT Suppressor of

Statement

Reference

kst[+]/kst² is a non-suppressor of lethal | recessive | partially | embryonic stage phenotype of peb³⁰⁸

(Wilk et al., 2004)

Phenotype Manifest In

Enhanced by

Statement

Reference

kst¹/kst² has eye photoreceptor cell stalk phenotype, enhanceable by crb^11A22/crb[+]

(Pellikka et al., 2002)

kst¹/kst² has rhabdomere | somatic clone phenotype, enhanceable by crb^11A22/crb[+]

(Pellikka et al., 2002)

NOT Enhanced by

Statement

Reference

kst⁰¹³¹⁸/kst² has follicle cell phenotype, non-enhanceable by sosie^f00514/CG13636[+]

(Urwyler et al., 2012)

NOT suppressed by

Statement

Reference

kst⁰¹³¹⁸/kst² has follicle cell phenotype, non-suppressible by sosie^f00514/CG13636[+]

(Urwyler et al., 2012)

Enhancer of

Statement

Reference

kst[+]/kst² is an enhancer of eye phenotype of Scer\GAL4^GMR.PFa, trio^UAS.cBa

(Lee and Thomas, 2011)

Other

Statement

Reference

Df(3R)sosie/+, kst² has follicle cell phenotype

(Urwyler et al., 2012)

crb^11A22, kst¹/kst² has rhabdomere | somatic clone phenotype

(Pellikka et al., 2002)

crb^11A22, kst¹/kst² has eye photoreceptor cell stalk phenotype

(Pellikka et al., 2002)

crb^11A22/crb[+], kst¹/kst² has rhabdomere | somatic clone phenotype

(Pellikka et al., 2002)

crb^11A22/crb[+], kst¹/kst² has eye photoreceptor cell stalk phenotype

(Pellikka et al., 2002)

Additional Comments

Genetic Interactions

Statement

Reference

Border cells migrate ahead of outer follicle cells in 65% of Df(3R)sosie/kst² double heterozygous egg chambers.

(Urwyler et al., 2012)

Heterozygosity for kst² significantly enhances the rough-eye phenotype induced by overexpression of trio^Scer\UAS.cBa via Scer\GAL4^GMR.PFa.

More kst²/Df(3L)1226 mutant larvae that are also heterozygous for Pak⁶ survive into pupal stages and beyond than kst²/Df(3L)1226 controls.

More kst²/kst^B1-14.1 mutant larvae that are also heterozygous for Pak⁶ survive into pupal stages and beyond than kst²/Df(3L)1226 controls.

(Lee and Thomas, 2011)

Introduction of one copy of kst² into a homozygous rho^ve-1 background moderately suppresses the wing vein defect for L2-L4 and partially suppresses the defects in L5.

(Tjota et al., 2011)

crb²/crb⁺, kst¹/kst² mutants have normal zonula adherens but photoreceptor stalk membranes are much shorter than in wild type, kst homozygous or crb heterozygous photoreceptor cells.

(Pellikka et al., 2002)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

99570

w^*; mwh¹ rho^ve-1 kst² red¹/TM6B, Tb⁺

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

kst2

(Thomas and Kiehart, 1995)

kst^2-2119

kst²

(Pinheiro et al., 2023, Urwyler et al., 2012, Lee and Thomas, 2011, Tjota et al., 2011, Richard et al., 2009, Phillips and Thomas, 2006)

Name Synonyms

Secondary FlyBase IDs

FBal0101351

References (14)

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