FB2024_03 , released June 25, 2024
Allele: Dmel\kstβH33.UAS
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General Information
Symbol
Dmel\kstβH33.UAS
Species
D. melanogaster
Name
FlyBase ID
FBal0256987
Feature type
allele
Associated gene
Dmel\kst
Associated Insertion(s)
Carried in Construct
P{UAS-kst.βH33}
Key Links
Transgenic product class
inframe_deletion
(Lee and Thomas, 2011)
Mutagen
Nature of the Allele
Transgenic product class
inframe_deletion
(Lee and Thomas, 2011)
Mutagen
in vitro construct
(Lee and Thomas, 2011)
Progenitor genotype
Carried in construct
P{UAS-kst.βH33}
Cytology
Description

UAS regulatory sequences drive expression of a C-terminal fragment of kst.

(Lee and Thomas, 2011)
Allele components
Component
Use(s)
Regulatory region(s)
UAS
Encoded product / tool
kst
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Expression of kstβH33.Scer\UAS under the control of Scer\GAL4GMR.PFa produces a strong rough-eye phenotype with dorsal posterior roughness, loss of pigmentation and often some necrosis.

      Sustained expression of kstβH33.Scer\UAS throughout eye development using the Scer\GAL4ey.200.Exel driver results in significant reduction in eye size and the occasional replacement of eye tissue with cuticle with bristle.

      Expression of kstβH33.Scer\UAS under the control of Scer\GAL4AB1 does not increase the diameter of the salivary gland lumen.

      (Lee and Thomas, 2011)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      Co-expression of kstβH33.Scer\UAS and trioScer\UAS.cBa under the control of Scer\GAL4GMR.PFa suppresses the roughness of both the kstβH33.Scer\UAS and the trioScer\UAS.cBa eye phenotype.

      Co-expression of tumdsRNA.Scer\UAS.cBa with kstβH33.Scer\UAS under the control of Scer\GAL4GMR.PFa suppresses the eye phenotype resulting from the expression of kstβH33.Scer\UAS alone.

      Co-expression of trioScer\UAS.cBa with kstβH33.Scer\UAS under the control of Scer\GAL4ey.200.Exel suppresses the eye phenotype resulting from the overexpression of kstβH33.Scer\UAS.

      Expression of kstβH33.Scer\UAS in combination with PakScer\UAS.T:Myr1 under the control of Scer\GAL4AB1 strongly suppresses the salivary gland lumen size defect caused by PakScer\UAS.T:Myr1 expression.

      (Lee and Thomas, 2011)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      kstβH33.Scer\UAS
      kstβH33.UAS
      Name Synonyms
      Secondary FlyBase IDs
        References (1)