FlyBase Allele Report: Dmel\msn[EP549]

General Information

Symbol

Dmel\msn^EP549

Species

D. melanogaster

Name

FlyBase ID

FBal0088791

Feature type

allele

Associated gene

Dmel\msn

Associated Insertion(s)

P{EP}msn^EP549

Carried in Construct

Also Known As

EP(3)549, ep(3)0549

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

P-element activity

Nature of the Allele

Allele class

Mutagen

P-element activity

(Rorth et al., 1998)

Progenitor genotype

Associated Insertion(s)

P{EP}msn^EP549

Cytology

Description

The P{EP} element is inserted 20 nucleotides 5' to the predicted ATG start codon.

(Kraut et al., 2001)

The P{EP} insertion is located 24bp upstream of the 5' end of a msn cDNA.

(Paricio et al., 1999)

P{EP} insertion in the 5' promoter region.

(Ruan et al., 1999)

Allele components

Component

Use(s)

Inserted element

P{EP}

misexpression element

Regulatory region(s)

UAS

binary expression system - regulatory region

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

transposable_element_insertion_site

3L:2,586,545..2,586,545 [-]

(Maybeck and Röper, 2009, Buff et al., 2007, Ruan et al., 2002, Gene Disruption Project members, 2001-, Kraut et al., 2001, Kraut et al., 2001, Paricio et al., 1999, Ruan et al., 1999, Rorth et al., 1998)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neuroanatomy, with Scer\GAL4^GMR.PF

(Ruan et al., 2002)

abnormal planar polarity, with Scer\GAL4^hs.2sev

(Paricio et al., 1999)

visible, with Scer\GAL4^ap-md544

(Paricio et al., 1999)

visible, with Scer\GAL4^hs.2sev

(Paricio et al., 1999)

Phenotype Manifest In

embryonic/larval salivary gland, with Scer\GAL4^fkh.PH

(Maybeck and Röper, 2009)

eye, with Scer\GAL4^hs.2sev

(Paricio et al., 1999)

eye photoreceptor cell, with Scer\GAL4^hs.2sev

(Paricio et al., 1999)

eye photoreceptor cell & axon, with Scer\GAL4^GMR.PF

(Ruan et al., 1999)

lamina plexus, with Scer\GAL4^GMR.PF

(Ruan et al., 2002)

larval intersegmental nerve, with Scer\GAL4^elav-C155

(Kraut et al., 2001)

medulla, with Scer\GAL4^GMR.PF

(Ruan et al., 2002, Ruan et al., 1999)

microchaeta & scutum, with Scer\GAL4^ap-md544

(Paricio et al., 1999)

ommatidial precursor cluster, with Scer\GAL4^hs.2sev

(Paricio et al., 1999)

ommatidium, with Scer\GAL4^hs.2sev

(Paricio et al., 1999)

photoreceptor cell R1 & axon, with Scer\GAL4^GMR.PF

(Ruan et al., 2002, Ruan et al., 1999)

photoreceptor cell R2 & axon, with Scer\GAL4^GMR.PF

(Ruan et al., 2002, Ruan et al., 1999)

photoreceptor cell R3 & axon, with Scer\GAL4^GMR.PF

(Ruan et al., 2002, Ruan et al., 1999)

photoreceptor cell R4 & axon, with Scer\GAL4^GMR.PF

(Ruan et al., 2002, Ruan et al., 1999)

photoreceptor cell R5 & axon, with Scer\GAL4^GMR.PF

(Ruan et al., 2002, Ruan et al., 1999)

photoreceptor cell R6 & axon, with Scer\GAL4^GMR.PF

(Ruan et al., 2002, Ruan et al., 1999)

photoreceptor cell R7 & axon, with Scer\GAL4^GMR.PF

(Ruan et al., 2002)

photoreceptor cell R8 & axon, with Scer\GAL4^GMR.PF

(Ruan et al., 2002)

rhabdomere, with Scer\GAL4^hs.2sev

(Paricio et al., 1999)

wing hair, with Scer\GAL4^ap-md544

(Paricio et al., 1999)

Detailed Description

Statement

Reference

Expression via Scer\GAL4^fkh.PH results in a weakly penetrant 'too wide and lumpy lumen' phenotype in the embryonic salivary gland.

(Maybeck and Röper, 2009)

Expression of msn^EP549 under the control of Scer\GAL4^GMR.PF has no affect on the eye.

(Müller et al., 2005)

In larvae expressing msn^EP549 under the control of Scer\GAL4^GMR.PF, nay R1-R6 growth cones terminate before they reach the lamina plexus. The regular array of R7 and R8 growth cones in the medulla is also disorganised. This phenotype is fully penetrant.

(Ruan et al., 2002)

Mutant larvae expressing msn^EP549 under the control of Scer\GAL4^elav-C155 have ISN neuron pathfinding defects.

(Kraut et al., 2001)

msn^EP549 flies, when driven by Scer\GAL4^ap-md544 show abnormal orientation of the microchaetae on the notum, wing hair polarity defects are also seen, along with duplication or triplication of some hairs. Over expression of msn^EP549 in the eye driven by Scer\GAL4^hs.2sev causes a rough eye phenotype and reveal typical polarity defects in ommatidia. Also some ommatidia have missing or malformed photoreceptors.

(Paricio et al., 1999)

Expression of msn^EP549 under the control of Scer\GAL4^GMR.PF causes a large number of R1-R6 photoreceptor cell growth cones to stop before they reach their normal target lamina. Defects are also seen in the medulla terminal field. The shape of the photoreceptor cells and their localisation on the developing eye disc is unaffected.

(Ruan et al., 1999)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Scer\GAL4^GMR.PF, msn^EP549 has abnormal neuroanatomy phenotype, enhanceable by Cdc42^unspecified

(Ruan et al., 2002)

Scer\GAL4^GMR.PF, msn^EP549 has abnormal neuroanatomy phenotype, enhanceable by Nrt^M54

(Ruan et al., 2002)

NOT Enhanced by

Statement

Reference

Scer\GAL4^GMR.PF, msn^EP549 has abnormal neuroanatomy phenotype, non-enhanceable by Rho1^E3.10

(Ruan et al., 2002)

Scer\GAL4^GMR.PF, msn^EP549 has abnormal neuroanatomy phenotype, non-enhanceable by bsk¹/bsk[+]

(Ruan et al., 2002)

Scer\GAL4^GMR.PF, msn^EP549 has abnormal neuroanatomy phenotype, non-enhanceable by chic⁰¹³²⁰

(Ruan et al., 2002)

Scer\GAL4^GMR.PF, msn^EP549 has abnormal neuroanatomy phenotype, non-enhanceable by Pak^unspecified

(Ruan et al., 2002)

Scer\GAL4^GMR.PF, msn^EP549 has abnormal neuroanatomy phenotype, non-enhanceable by Rac1^J11/Rac2^Δ/Mtl^Δ

(Ruan et al., 2002)

Scer\GAL4^hs.2sev, msn^EP549 has abnormal planar polarity phenotype, non-enhanceable by dock³

(Paricio et al., 1999)

Scer\GAL4^hs.2sev, msn^EP549 has abnormal planar polarity phenotype, non-enhanceable by Df(3L)Ar14-8

(Paricio et al., 1999)

Scer\GAL4^hs.2sev, msn^EP549 has abnormal planar polarity phenotype, non-enhanceable by Rho1^72O

(Paricio et al., 1999)

Suppressed by

Statement

Reference

Scer\GAL4^GMR.PF, msn^EP549 has abnormal neuroanatomy phenotype, suppressible by bif[+]/bif^R47

(Ruan et al., 2002)

Scer\GAL4^GMR.PF, msn^EP549 has abnormal neuroanatomy phenotype, suppressible by bif^R38/bif[+]

(Ruan et al., 2002)

Scer\GAL4^hs.2sev, msn^EP549 has abnormal planar polarity phenotype, suppressible by bsk²/bsk[+]

(Paricio et al., 1999)

Scer\GAL4^hs.2sev, msn^EP549 has abnormal planar polarity phenotype, suppressible by hep[+]/hep^r75

(Paricio et al., 1999)

Scer\GAL4^hs.2sev, msn^EP549 has abnormal planar polarity phenotype, suppressible by Jra^IA109/Jra[+]

(Paricio et al., 1999)

Scer\GAL4^hs.2sev, msn^EP549 has abnormal planar polarity phenotype, suppressible by Df(1)H6/+

(Paricio et al., 1999)

Scer\GAL4^hs.2sev, msn^EP549 has abnormal planar polarity phenotype, suppressible by Df(1)G24/+

(Paricio et al., 1999)

Scer\GAL4^hs.2sev, msn^EP549 has abnormal planar polarity phenotype, suppressible by Df(2L)flp170B/+

(Paricio et al., 1999)

Scer\GAL4^hs.2sev, msn^EP549 has abnormal planar polarity phenotype, suppressible by Df(3R)crb87-4/+

(Paricio et al., 1999)

Scer\GAL4^hs.2sev, msn^EP549 has abnormal planar polarity phenotype, suppressible by Df(2L)b80e3/+

(Paricio et al., 1999)

Scer\GAL4^hs.2sev, msn^EP549 has abnormal planar polarity phenotype, suppressible by Df(3R)p13/+

(Paricio et al., 1999)

Scer\GAL4^hs.2sev, msn^EP549 has abnormal planar polarity phenotype, suppressible by Df(3R)crb-F89-4/+

(Paricio et al., 1999)

Scer\GAL4^hs.2sev, msn^EP549 has abnormal planar polarity phenotype, suppressible by Df(2L)b87e25/+

(Paricio et al., 1999)

NOT suppressed by

Statement

Reference

Scer\GAL4^GMR.PF, msn^EP549 has abnormal neuroanatomy phenotype, non-suppressible by Pak^unspecified

(Ruan et al., 2002)

Scer\GAL4^GMR.PF, msn^EP549 has abnormal neuroanatomy phenotype, non-suppressible by Rac1^J11/Rac2^Δ/Mtl^Δ

(Ruan et al., 2002)

Scer\GAL4^GMR.PF, msn^EP549 has abnormal neuroanatomy phenotype, non-suppressible by Rho1^E3.10

(Ruan et al., 2002)

Scer\GAL4^GMR.PF, msn^EP549 has abnormal neuroanatomy phenotype, non-suppressible by bsk¹/bsk[+]

(Ruan et al., 2002)

Scer\GAL4^GMR.PF, msn^EP549 has abnormal neuroanatomy phenotype, non-suppressible by chic⁰¹³²⁰

(Ruan et al., 2002)

Scer\GAL4^hs.2sev, msn^EP549 has abnormal planar polarity phenotype, non-suppressible by dock³

(Paricio et al., 1999)

Scer\GAL4^hs.2sev, msn^EP549 has abnormal planar polarity phenotype, non-suppressible by Df(3L)Ar14-8

(Paricio et al., 1999)

Scer\GAL4^hs.2sev, msn^EP549 has abnormal planar polarity phenotype, non-suppressible by Rho1^72O

(Paricio et al., 1999)

Enhancer of

Statement

Reference

msn^EP549, Scer\GAL4^GMR.PF is an enhancer of visible phenotype of Ppt1^UAS.cKa, Scer\GAL4^GMR.PF

(Buff et al., 2007)

msn^EP549/Scer\GAL4^sca-537.4 is an enhancer of visible phenotype of Rala^35d

(Balakireva et al., 2006)

msn^EP549, Scer\GAL4^GMR.PF is an enhancer of visible phenotype of H^UAS.cMa, Scer\GAL4^GMR.PF

(Müller et al., 2005)

Phenotype Manifest In

Enhanced by

Statement

Reference

Scer\GAL4^GMR.PF, msn^EP549 has photoreceptor cell & axon phenotype, enhanceable by Cdc42^unspecified

(Ruan et al., 2002)

Scer\GAL4^GMR.PF, msn^EP549 has photoreceptor cell & axon phenotype, enhanceable by Nrt^M54

(Ruan et al., 2002)

NOT Enhanced by

Statement

Reference

Scer\GAL4^GMR.PF, msn^EP549 has photoreceptor cell & axon phenotype, non-enhanceable by Rho1^E3.10

(Ruan et al., 2002)

Scer\GAL4^GMR.PF, msn^EP549 has photoreceptor cell & axon phenotype, non-enhanceable by bsk¹/bsk[+]

(Ruan et al., 2002)

Scer\GAL4^GMR.PF, msn^EP549 has photoreceptor cell & axon phenotype, non-enhanceable by chic⁰¹³²⁰

(Ruan et al., 2002)

Scer\GAL4^GMR.PF, msn^EP549 has photoreceptor cell & axon phenotype, non-enhanceable by Pak^unspecified

(Ruan et al., 2002)

Scer\GAL4^GMR.PF, msn^EP549 has photoreceptor cell & axon phenotype, non-enhanceable by Rac1^J11/Rac2^Δ/Mtl^Δ

(Ruan et al., 2002)

Scer\GAL4^hs.2sev, msn^EP549 has ommatidium phenotype, non-enhanceable by dock³

(Paricio et al., 1999)

Scer\GAL4^hs.2sev, msn^EP549 has ommatidium phenotype, non-enhanceable by Df(3L)Ar14-8

(Paricio et al., 1999)

Scer\GAL4^hs.2sev, msn^EP549 has ommatidium phenotype, non-enhanceable by Rho1^72O

(Paricio et al., 1999)

Suppressed by

Statement

Reference

Scer\GAL4^GMR.PF, msn^EP549 has photoreceptor cell & axon phenotype, suppressible by bif^R38/bif[+]

(Ruan et al., 2002)

Scer\GAL4^GMR.PF, msn^EP549 has photoreceptor cell & axon phenotype, suppressible by bif[+]/bif^R47

(Ruan et al., 2002)

Scer\GAL4^hs.2sev, msn^EP549 has ommatidium phenotype, suppressible by bsk²

(Paricio et al., 1999)

Scer\GAL4^hs.2sev, msn^EP549 has ommatidium phenotype, suppressible by hep^r75

(Paricio et al., 1999)

Scer\GAL4^hs.2sev, msn^EP549 has ommatidium phenotype, suppressible by Jra^IA109

(Paricio et al., 1999)

Scer\GAL4^hs.2sev, msn^EP549 has ommatidium phenotype, suppressible by Df(1)H6

(Paricio et al., 1999)

Scer\GAL4^hs.2sev, msn^EP549 has ommatidium phenotype, suppressible by Df(1)G24

(Paricio et al., 1999)

Scer\GAL4^hs.2sev, msn^EP549 has ommatidium phenotype, suppressible by Df(2L)flp170B

(Paricio et al., 1999)

Scer\GAL4^hs.2sev, msn^EP549 has ommatidium phenotype, suppressible by Df(3R)crb87-4

(Paricio et al., 1999)

Scer\GAL4^hs.2sev, msn^EP549 has ommatidium phenotype, suppressible by Df(2L)b80e3

(Paricio et al., 1999)

Scer\GAL4^hs.2sev, msn^EP549 has ommatidium phenotype, suppressible by Df(3R)p13

(Paricio et al., 1999)

Scer\GAL4^hs.2sev, msn^EP549 has ommatidium phenotype, suppressible by Df(3R)crb-F89-4

(Paricio et al., 1999)

Scer\GAL4^hs.2sev, msn^EP549 has ommatidium phenotype, suppressible by Df(2L)b87e25

(Paricio et al., 1999)

Scer\GAL4^GMR.PF, msn^EP549 has eye photoreceptor cell & axon phenotype, suppressible by Scer\GAL4^GMR.PF/dock^UAS.cRa

(Ruan et al., 1999)

Scer\GAL4^GMR.PF, msn^EP549 has eye photoreceptor cell & axon phenotype, suppressible by dock^W225K.UAS/Scer\GAL4^GMR.PF

(Ruan et al., 1999)

Scer\GAL4^GMR.PF, msn^EP549 has eye photoreceptor cell & axon phenotype, suppressible | partially by dock^W48K.UAS/Scer\GAL4^GMR.PF

(Ruan et al., 1999)

NOT suppressed by

Statement

Reference

Scer\GAL4^GMR.PF, msn^EP549 has photoreceptor cell & axon phenotype, non-suppressible by Pak^unspecified

(Ruan et al., 2002)

Scer\GAL4^GMR.PF, msn^EP549 has photoreceptor cell & axon phenotype, non-suppressible by Rac1^J11/Rac2^Δ/Mtl^Δ

(Ruan et al., 2002)

Scer\GAL4^GMR.PF, msn^EP549 has photoreceptor cell & axon phenotype, non-suppressible by Rho1^E3.10

(Ruan et al., 2002)

Scer\GAL4^GMR.PF, msn^EP549 has photoreceptor cell & axon phenotype, non-suppressible by bsk¹/bsk[+]

(Ruan et al., 2002)

Scer\GAL4^GMR.PF, msn^EP549 has photoreceptor cell & axon phenotype, non-suppressible by chic⁰¹³²⁰

(Ruan et al., 2002)

Scer\GAL4^hs.2sev, msn^EP549 has ommatidium phenotype, non-suppressible by dock³

(Paricio et al., 1999)

Scer\GAL4^hs.2sev, msn^EP549 has ommatidium phenotype, non-suppressible by Df(3L)Ar14-8

(Paricio et al., 1999)

Scer\GAL4^hs.2sev, msn^EP549 has ommatidium phenotype, non-suppressible by Rho1^72O

(Paricio et al., 1999)

Scer\GAL4^GMR.PF, msn^EP549 has eye photoreceptor cell & axon phenotype, non-suppressible by dock^W151K.UAS/Scer\GAL4^GMR.PF

(Ruan et al., 1999)

Scer\GAL4^GMR.PF, msn^EP549 has eye photoreceptor cell & axon phenotype, non-suppressible by Scer\GAL4^GMR.PF/dock^R336Q.UAS

(Ruan et al., 1999)

Enhancer of

Statement

Reference

msn^EP549, Scer\GAL4^GMR.PF is an enhancer of eye phenotype of Ppt1^UAS.cKa, Scer\GAL4^GMR.PF

(Buff et al., 2007)

msn^EP549/Scer\GAL4^sca-537.4 is an enhancer of microchaeta phenotype of Rala^35d

(Balakireva et al., 2006)

msn^EP549/Scer\GAL4^sca-537.4 is an enhancer of macrochaeta phenotype of Rala^35d

(Balakireva et al., 2006)

msn^EP549, Scer\GAL4^GMR.PF is an enhancer of eye phenotype of H^UAS.cMa, Scer\GAL4^GMR.PF

(Müller et al., 2005)

Suppressor of

Statement

Reference

msn^EP549/Scer\GAL4^GMR.PF is a suppressor of eye photoreceptor cell & axon phenotype of dock⁰⁴⁷²³

(Ruan et al., 1999)

msn^EP549/Scer\GAL4^GMR.PF is a suppressor of eye photoreceptor cell & growth cone phenotype of dock⁰⁴⁷²³

(Ruan et al., 1999)

Additional Comments

Genetic Interactions

Statement

Reference

The defects in photoreceptor cell projection patterns seen in larvae overexpressing msn^EP549 under the control of Scer\GAL4^GMR.PF are largely suppressed by one copy of bif^R38 or one copy of bif^R47. The defects in photoreceptor cell projection patterns seen in larvae overexpressing msn^EP549 under the control of Scer\GAL4^GMR.PF are not affected by one copy of bsk¹. The defects in photoreceptor cell projection patterns seen in larvae overexpressing msn^EP549 under the control of Scer\GAL4^GMR.PF are enhanced by Cdc42^unspecified or Nrt^M54. The phenotype caused by expression of msn^EP549 under the control of Scer\GAL4^GMR.PF is not modified by the addition of Pak^unspecified, Rho1^E3.10 or chic⁰¹³²⁰. The phenotype caused by expression of msn^EP549 under the control of Scer\GAL4^GMR.PF is not modified by the addition of the triple mutant combination Rac1^J11 Rac2^Δ Mtl^Δ.

(Ruan et al., 2002)

The phenotypes caused by over-expression of msn^EP549 in the eye driven by Scer\GAL4^hs.2sev are dominantly suppressed by bsk², hep^r75, Jra^IA109, Df(1)H6, Df(1)G24, Df(2L)flp170B, Df(3R)crb87-4, Df(2L)b80e3, Df(3R)p13, Df(3R)crb-F89-4 and Df(2L)b87e25, leading to almost wild-type ommatidial arrays. The addition of dock³, Df(3L)Ar14-8 and Rho1^72O have no effect.

(Paricio et al., 1999)

Co-expression of dock^Scer\UAS.cRa or dock^{W225K.Scer\UAS} suppresses the pretarget termination phenotype of photoreceptor cell axons caused by expression of msn^EP549 under the control of Scer\GAL4^GMR.PF. Co-expression of dock^{W48K.Scer\UAS} weakly suppresses the pretarget termination phenotype of photoreceptor cell axons caused by expression of msn^EP549 under the control of Scer\GAL4^GMR.PF. Co-expression of dock^{W151K.Scer\UAS} or dock^{R336Q.Scer\UAS} does not suppress the pretarget termination phenotype of photoreceptor cell axons caused by expression of msn^EP549 under the control of Scer\GAL4^GMR.PF. The dock⁰⁴⁷²³ phenotype is largely suppressed by msn^EP549 expressed under the control of Scer\GAL4^GMR.PF. The premature termination of many photoreceptor cell growth cones within the optic stalk is also seen in these larvae (this phenotype is not seen in larvae expressing msn^EP549 under the control of Scer\GAL4^GMR.PF in a wild-type background).

(Ruan et al., 1999)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

Selected as: an insertion line that modifies the eye phenotype caused by expression of Ppt1^Scer\UAS.cKa under the control of Scer\GAL4^GMR.PF when co-expressed using Scer\GAL4^GMR.PF.

(Buff et al., 2007)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

EP(3)549

(Ruan et al., 1999)

ep(3)0549

(Paricio et al., 1999)

msn^EP549

(Balakireva et al., 2006)

Name Synonyms

Secondary FlyBase IDs

References (11)

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