FB2024_04 , released June 25, 2024
Recombinant Construct: P{EP}
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General Information
Symbol
P{EP}
FlyBase ID
FBtp0001317
Feature type
transgenic_transposable_element
Component Allele(s)
Size
7.987
Expression Data
Associated insertion(s)
Molecular map
Description and Uses
Description

The P{EP} element contains UAS regulatory sequences and Hsp70 promoter sequence (-45 to +33) at its 3' end, oriented so that in the presence of a GAL4 driver, transcription from the promoter is directed outwards from this end of the element. The P{EP} element typically inserts at the 5' end of genes, resulting in over- or mis-expression of the endogenous gene in the presence of a GAL4 driver. A set of GAGA binding sites are inserted upstream of the UAS sequences to prevent position effects. A w+mC marker is present at the 5' end of the element.

A diagram of the P{EP} element is available at the [GDP Transposons page](http://flypush.imgen.bcm.tmc.edu/pscreen/transposons.html)

Construct components
Component
Use(s)
Component allele TrlBRcRa
Transgenic product class
Component
Use(s)
Encoded product / tool
Description

Construct: Contains two copies of a 'GAGA element' which can bind Trl.

Sequence Data
Sequence (FB)
Associated Sequence Data
DNA Sequence
Extent
Segments and Size
Total Size

7.987

Left end
Right end
 
Reference
Phenotypic Data
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Progenitors and Descendants
Comments

Restriction site useful for plasmid rescue: EcoRI.

Stocks (2,896)
Stocks containing insertion(s) of this recombinant construct
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (2)
Reported As
Secondary FlyBase IDs
    References (219)