FlyBase Allele Report: Dmel\Jra[IA109]

General Information

Symbol

Dmel\Jra^IA109

Species

D. melanogaster

Name

FlyBase ID

FBal0009252

Feature type

allele

Associated gene

Dmel\Jra

Associated Insertion(s)

Carried in Construct

Also Known As

jun², Djun², jun^IA109, Djun^IA109

Key Links

Genomic Maps

JBrowse

Allele class

amorphic allele - genetic evidence

loss of function allele

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

amorphic allele - genetic evidence

(Hou et al., 1997, Riesgo-Escovar and Hafen, 1997)

loss of function allele

(Kockel et al., 1997)

Mutagen

ethyl methanesulfonate

(Tearle and Nusslein-Volhard, 1987, Nusslein-Volhard et al., 1984)

Progenitor genotype

Cytology

Description

Stop codon at amino acid position 72 resulting in a predicted protein that terminates immediately after the amino-terminal "Delta" domain of the protein.

(Hou et al., 1997)

Stop codon at amino acid 72.

(Kockel et al., 1997)

Nucleotide substitution: ?651?.

(Riesgo-Escovar and Hafen, 1997)

Encodes a truncated protein lacking the DNA binding and dimerization domain and the amino-terminal phosphorylation sites.

(Riesgo-Escovar and Hafen, 1997)

Amino acid replacement: K71term.

(Riesgo-Escovar and Hafen, 1997)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

2R:10,097,480..10,097,480 [+]

Nucleotide change:

A10097480T

Amino acid change:

K72term | Jra-PA; K72term | Jra-PB; K72term | Jra-PC

(Hou et al., 1997)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal planar polarity | somatic clone

(Weber et al., 2000)

lethal - all die during embryonic stage | recessive

(Hou et al., 1997, Kockel et al., 1997, Riesgo-Escovar and Hafen, 1997, Staehling-Hampton et al., 1995, Tearle and Nusslein-Volhard, 1987, Nusslein-Volhard et al., 1984)

some die during embryonic stage | recessive

(Hou et al., 1997, Kockel et al., 1997, Riesgo-Escovar and Hafen, 1997, Staehling-Hampton et al., 1995, Tearle and Nusslein-Volhard, 1987, Nusslein-Volhard et al., 1984)

Phenotype Manifest In

Detailed Description

Statement

Reference

Mass ablation of midline precursor cells in homozygous embryos does not activate midline cell division, in contrast to the response seen in wild-type embryos. After ablation of a single midline sibling in the mutant embryo, the surviving sibling does not divide or differentiate in 57% of cases, and replacement of the ablated sibling cell is rare (14% of cases).

(Bossing et al., 2012)

Jra^IA109 neuroblast clones in the mushroom body have no gross axonal phenotypes.

(Rallis et al., 2010)

Mutant flies show a higher bacterial clearance activity than do control flies after injection with E.coli.

Heterozygous flies show a lower survival rate after infection with E.coli than do control flies.

(Kim et al., 2007)

Heterozygous adults treated with hydrogen peroxide at the onset of metamorphosis do not show any defects.

(Jindra et al., 2004)

Homozygous embryos have a dorsal open phenotype.

(Ciapponi and Bohmann, 2002)

Dorsal closure of the ectoderm does not occur in mutant embryos. The heart is most often found near the dorsal midline in these embryos and appears to initiate its own "dorsal closure" independently of the ectoderm.

(Lockwood and Bodmer, 2002)

Eggs derived from females with homozygous clones in the follicle cells show a variety of dorsal appendage defects ranging from partial to complete absence of elongation and an accompanying expansion of their bases. The micropyle is reduced in size and sometimes has a truncated shape, looking incompletely "closed".

(Suzanne et al., 2001)

In large clones in the eye many ommatidia display correct polarity though others show chirality flips or symmetry of the R4/R4 cell type. Clusters are occasionally misrotated and photoreceptor cells are occasionally missing, though this phenotype has low penetrance.

(Weber et al., 2000)

Embryos fail to show complete elongation of the dorsal epidermis after germband retraction.

(Ricos et al., 1999)

Embryos exhibit a dorsal open phenotype (dorsal closure is not initiated) and head defects.

(Hou et al., 1997)

Dorsal closure defect. Mitotic clones in the eye cause no abnormal defects in eye development. Microscopic inspection of sections reveals minor defects, the occasional missing photoreceptor.

(Kockel et al., 1997)

Embryos exhibit a dorsal open phenotype caused by failure of the lateral epithelial cells to stretch. Mitotic clones induced in the eye exhibit normal number and arrangement of photoreceptors.

(Riesgo-Escovar and Hafen, 1997)

Embryos show an dorsal open phenotype.

(Staehling-Hampton et al., 1995)

Jra^IA109 embryos have a dorsal anterior hole.

(Tearle and Nusslein-Volhard, 1987)

Dorsal anterior hole.

(Nusslein-Volhard et al., 1984)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Jra^IA109 has lethal | recessive phenotype, suppressible by Jra::kay^JFJ.hs.sev

(Ciapponi and Bohmann, 2002)

NOT suppressed by

Statement

Reference

Jra^IA109 has lethal | recessive phenotype, non-suppressible by Jra::kay^FJF.hs.sev

(Ciapponi and Bohmann, 2002)

NOT Enhancer of

Statement

Reference

Jra^IA109/Jra[+] is a non-enhancer of abnormal planar polarity phenotype of Mtl^UAS.cMa, Scer\GAL4^hs.2sev

(Muñoz-Descalzo et al., 2007)

Jra^IA109 is a non-enhancer of abnormal cell polarity phenotype of Scer\GAL4^hs.2sev, pk^sple.UAS

(Jenny et al., 2003)

Suppressor of

Statement

Reference

Jra^IA109/Jra[+] is a suppressor of abnormal planar polarity phenotype of dsh^hs.sev.B

(Weber et al., 2008)

Jra^IA109/Jra[+] is a suppressor of visible | dominant phenotype of hep^CA

(Miotto et al., 2006)

Jra^IA109/Jra[+] is a suppressor of visible | dominant phenotype of sd^SG29.1

(Miotto et al., 2006)

Jra^IA109/Jra[+] is a suppressor | partially of abnormal neuroanatomy phenotype of Hsap\APP^UAS.Tag:MYC, Scer\GAL4^P2.4.Pdf

(Leyssen et al., 2005)

Jra^IA109 is a suppressor of visible phenotype of Ret^MEN2B.GMR

(Read et al., 2005)

Jra^IA109 is a suppressor of visible phenotype of Ret^MEN2A.GMR

(Read et al., 2005)

Jra^IA109 is a suppressor of visible phenotype of Ret^GMR.PR

(Read et al., 2005)

Jra^IA109/Jra[+] is a suppressor | partially of abnormal planar polarity phenotype of Scer\GAL4^hs.2sev, fz^UAS.cAa

(Weber et al., 2000)

Jra^IA109/Jra[+] is a suppressor of abnormal planar polarity phenotype of Scer\GAL4^hs.2sev, msn^EP549

(Paricio et al., 1999)

Jra^IA109/Jra[+] is a suppressor of visible phenotype of faf^BX4

(Isaksson et al., 1997)

NOT Suppressor of

Statement

Reference

Jra^IA109/Jra[+] is a non-suppressor of abnormal neuroanatomy | larval stage phenotype of Eaat1^hypo

(Peng et al., 2019)

Jra^IA109/Jra[+] is a non-suppressor of abnormal planar polarity phenotype of Mtl^UAS.cMa, Scer\GAL4^hs.2sev

(Muñoz-Descalzo et al., 2007)

Jra^IA109 is a non-suppressor of lethal | pupal stage phenotype of Csk^j1D8/Csk^S030003

(Read et al., 2004)

Jra^IA109 is a non-suppressor of abnormal cell polarity phenotype of Scer\GAL4^hs.2sev, pk^sple.UAS

(Jenny et al., 2003)

Other

Statement

Reference

Jra^IA109, mbf1² has visible phenotype

(Jindra et al., 2004)

Phenotype Manifest In

Suppressed by

Statement

Reference

Jra^IA109 has embryonic dorsal epidermis phenotype, suppressible by Jra::kay^JFJ.hs.sev

(Ciapponi and Bohmann, 2002)

NOT suppressed by

Statement

Reference

Jra^IA109 has embryonic dorsal epidermis phenotype, non-suppressible by Jra::kay^FJF.hs.sev

(Ciapponi and Bohmann, 2002)

Enhancer of

Statement

Reference

Jra^IA109 is an enhancer of embryonic epidermis | dorsal phenotype of kay²

(Riesgo-Escovar and Hafen, 1997)

NOT Enhancer of

Statement

Reference

Jra^IA109/Jra[+] is a non-enhancer of ommatidium phenotype of Mtl^UAS.cMa, Scer\GAL4^hs.2sev

(Muñoz-Descalzo et al., 2007)

Jra^IA109/Jra[+] is a non-enhancer of adult thorax | pharate adult stage phenotype of chm¹⁴

(Miotto et al., 2006)

Jra^IA109 is a non-enhancer of ommatidium phenotype of Scer\GAL4^hs.2sev, pk^sple.UAS

(Jenny et al., 2003)

Suppressor of

Statement

Reference

Jra^IA109/Jra[+] is a suppressor of germline cell | embryonic stage 15 phenotype of raw^155.27/raw^134.47

(Jemc et al., 2012)

Jra^IA109/Jra[+] is a suppressor of ommatidium phenotype of dsh^hs.sev.B

(Weber et al., 2008)

Jra^IA109/Jra[+] is a suppressor of wing phenotype of hep^CA

(Miotto et al., 2006)

Jra^IA109/Jra[+] is a suppressor | partially of ventral adult lateral neuron & axon phenotype of Hsap\APP^UAS.Tag:MYC, Scer\GAL4^P2.4.Pdf

(Leyssen et al., 2005)

Jra^IA109 is a suppressor of eye phenotype of Ret^MEN2B.GMR

(Read et al., 2005)

Jra^IA109 is a suppressor of eye phenotype of Ret^MEN2A.GMR

(Read et al., 2005)

Jra^IA109 is a suppressor of eye phenotype of Ret^GMR.PR

(Read et al., 2005)

Jra^IA109/Jra[+] is a suppressor | partially of ommatidium phenotype of peb¹

(Pickup et al., 2002)

Jra^IA109 is a suppressor of ommatidium phenotype of Rac1^V12.hs.sev

(Fanto et al., 2000)

Jra^IA109/Jra[+] is a suppressor | partially of ommatidium phenotype of Scer\GAL4^hs.2sev, fz^UAS.cAa

(Weber et al., 2000)

Jra^IA109/Jra[+] is a suppressor | partially of photoreceptor cell R4 phenotype of Scer\GAL4^hs.2sev, fz^UAS.cAa

(Weber et al., 2000)

Jra^IA109 is a suppressor of embryonic/first instar larval cuticle | ventral phenotype of raw¹

(Byars et al., 1999)

Jra^IA109 is a suppressor of ommatidium phenotype of Scer\GAL4^hs.2sev, msn^EP549

(Paricio et al., 1999)

Jra^IA109 is a suppressor of phenotype of dsh^hs.sev.B

(Boutros et al., 1998)

Jra^IA109/Jra[+] is a suppressor of photoreceptor cell R1 | ectopic phenotype of faf^BX4

(Isaksson et al., 1997)

Jra^IA109/Jra[+] is a suppressor of photoreceptor cell R2 | ectopic phenotype of faf^BX4

(Isaksson et al., 1997)

Jra^IA109/Jra[+] is a suppressor of photoreceptor cell R3 | ectopic phenotype of faf^BX4

(Isaksson et al., 1997)

Jra^IA109/Jra[+] is a suppressor of photoreceptor cell R4 | ectopic phenotype of faf^BX4

(Isaksson et al., 1997)

Jra^IA109/Jra[+] is a suppressor of photoreceptor cell R5 | ectopic phenotype of faf^BX4

(Isaksson et al., 1997)

Jra^IA109/Jra[+] is a suppressor of photoreceptor cell R6 | ectopic phenotype of faf^BX4

(Isaksson et al., 1997)

Jra^IA109 is a suppressor of wing phenotype of Egfr^E3

(Kockel et al., 1997)

Jra^IA109 is a suppressor of eye photoreceptor cell | ectopic phenotype of rl^Sem

(Kockel et al., 1997)

Jra^IA109/Jra[+] is a suppressor of eye photoreceptor cell phenotype of sev^S11.Tag:MYC

(Kockel et al., 1997)

NOT Suppressor of

Statement

Reference

Jra^IA109/Jra[+] is a non-suppressor of embryonic/larval neuromuscular junction | larval stage phenotype of Eaat1^hypo

(Peng et al., 2019)

Jra^IA109/Jra[+] is a non-suppressor of NMJ bouton | increased number | larval stage phenotype of Eaat1^hypo

(Peng et al., 2019)

Jra^IA109/Jra[+] is a non-suppressor of ommatidium phenotype of Mtl^UAS.cMa, Scer\GAL4^hs.2sev

(Muñoz-Descalzo et al., 2007)

Jra^IA109 is a non-suppressor of ommatidium phenotype of Scer\GAL4^hs.2sev, pk^sple.UAS

(Jenny et al., 2003)

Jra^IA109 is a non-suppressor of eye phenotype of Scer\GAL4^GMR.PF, egr^GS9830

(Igaki et al., 2002)

Other

Statement

Reference

Jra^IA109, mbf1² has adult cuticle phenotype

(Jindra et al., 2004)

Additional Comments

Genetic Interactions

Statement

Reference

Jra^IA109/+ suppresses the frequency of ensheathment defects in raw^134.47/raw^155.27 mutants.

(Jemc et al., 2012)

One copy of Jra^IA109 suppresses the formation of symmetrical ommatidia that is seen in animals expressing dsh^hs.sev.B.

(Weber et al., 2008)

The lower survival rate seen in Jra^IA109 heterozygous flies after infection with E.coli is suppressed if the flies are also heterozygous for Rel^E20.

(Kim et al., 2007)

Jra^IA109 does not protect Df(1)su(s)R194/+ clones in the eye; Df(1)su(s)R194/+ ; Jra^IA109 clones are not recovered in the adult eye in animals with mosaic eyes containing two genotypes of cells with respect to RpL36; cells which are Df(1)su(s)R194/+ and cells in which the haplo-insufficiency of Df(1)su(s)R194/+ for RpL36 has been rescued by RpL36^+t4 (in a wild-type background the Df(1)su(s)R194/+ clones are eliminated by cell competition and are not seen in the adult eye in these animals). Also, the proportion of Df(1)su(s)R194/+ cells in the larval eye disc is not increased by the presence of Jra^IA109.

(Tyler et al., 2007)

Jra^IA109/+ ; mbf1² double mutant adults treated with hydrogen peroxide at the onset of metamorphosis often have cuticle defects, which manifest as a depressed patch of naked cuticle at the dorsal midline.

(Jindra et al., 2004)

The dorsal open phenotype of Jra^IA109 homozygous embryos is not rescued by Jra::kay^FJF.hs.sev. The dorsal open phenotype of Jra^IA109 homozygous embryos is significantly rescued by Jra::kay^JFJ.hs.sev.

(Ciapponi and Bohmann, 2002)

The addition of Jra^IA109 to egr^GS9830, Scer\GAL4^GMR.PF animals fails to suppress the eye phenotype.

(Igaki et al., 2002)

The ventral cuticle in raw¹ Jra^IA109 double mutant embryos develops normally, as it does in Jra^IA109 single mutants.

(Byars et al., 1999)

Dominantly suppresses the ommatidial polarity phenotype seen in flies with msn^EP549 driven by Scer\GAL4^hs.2sev, producing near wild-type ommatidial arrays.

(Paricio et al., 1999)

The extra outer photoreceptor cell phenotype seen in faf^BX4 homozygotes is dominantly suppressed by Jra^IA109, with the average number of outer photoreceptor cells per ommatidium being reduced to 6.8.

(Isaksson et al., 1997)

Xenogenetic Interactions

Statement

Reference

Heterozygosity for Jra^IA109 partially suppresses the Scer\GAL4^P2.4.Pdf>Hsap\APP^{Scer\UAS.T:Hsap\MYC} sLNv axonal arborization phenotype.

(Leyssen et al., 2005)

Complementation and Rescue Data

Rescued by

Jra^IA109 is rescued by Jra^+t5.2

(Hou et al., 1997)

Partially rescued by

Jra^IA109 is partially rescued by Jra^hs.sev

(Riesgo-Escovar and Hafen, 1997)

Comments

Heat induced expression of Jra^hs.sev rescues the embryos to pharate adults.

(Riesgo-Escovar and Hafen, 1997)

Images (0)

Mutant

Wild-type

Stocks (2)

Bloomington

3273

cn¹ Jra^IA109 bw¹ speck¹/CyO

Kyoto

106951

cn¹ Jra^IA109 bw¹ speck¹/CyO

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (19)

Reported As

Symbol Synonym

(Weber et al., 2012)

D-jun²

(Isaksson et al., 1997)

DJun^IA109

(Riesgo-Escovar and Hafen, 1997)

Djun²

(Suzanne et al., 2001, Fanto, 2000, Fanto et al., 2000, McEwen et al., 2000, Hou et al., 1997)

Djun^IA109

(Miotto et al., 2006, Ricos et al., 1999)

I(2R)IA 109

(Lockwood and Bodmer, 2002)

IA109

(Weber et al., 2012)

Jra^1A109

(Kim et al., 2007)

Jra²

(Rallis et al., 2010)

Jra^IA109

(Soory and Ratnaparkhi, 2022, Bossing et al., 2012, Tokusumi et al., 2009, Christensen and Cook, 2007.5.8, Mace et al., 2005)

Jun^1A189

(Schimmelpfeng et al., 2006)

Jun²

(Rallis et al., 2010)

Jun^IA109

(Calderon et al., 2022)

dJun²

(Delaney et al., 2006)

jra^IA109

(Peng et al., 2019, Beira et al., 2014, Jemc et al., 2012, Neuman-Silberberg, 2007)

jun²

(Weber et al., 2008, Muñoz-Descalzo et al., 2007, Tyler et al., 2007, Munoz-Descalzo et al., 2005, Jenny et al., 2003, Ciapponi and Bohmann, 2002, Igaki et al., 2002, Pickup et al., 2002, Kockel et al., 2001, Mihaly et al., 2001, Paricio et al., 1999, Boutros et al., 1998, Kockel et al., 1997)

jun^IA109

(Weber et al., 2008, Polaski et al., 2006, Weber et al., 2000)

l(2)IA109

(Hou et al., 1997)

l(2)IA109¹

(Staehling-Hampton et al., 1995)

Name Synonyms

Secondary FlyBase IDs

References (50)

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