FlyBase Allele Report: Dmel\jus[iso7.8]

General Information

Symbol

Dmel\jus^iso7.8

Species

D. melanogaster

Name

FlyBase ID

FBal0060899

Feature type

allele

Associated gene

Dmel\jus

Associated Insertion(s)

Carried in Construct

Also Known As

sda^iso7.8, Slamdance^iso7.8

Key Links

Allele class

Mutagen

spontaneous

Nature of the Allele

Allele class

Mutagen

spontaneous

(Zhang et al., 2002)

Progenitor genotype

Cytology

Description

2bp insertion in exon III between nucleotides 671 and 672 in the 5' untranslated region.

(Zhang et al., 2002)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Disease

Evidence

References

model of epilepsy

CEA

(Byers et al., 2021, Jeong et al., 2021, Dean et al., 2018, Kroll et al., 2015, Howlett and Tanouye, 2013, Marley and Baines, 2011, Tan et al., 2004)

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

model of epilepsy

is ameliorated by shi¹

(Kroll et al., 2015)

is ameliorated by shi²

(Kroll et al., 2015)

is exacerbated by SLO2^CRISPR

(Byers et al., 2021)

is exacerbated by SLO2^DN.UAS

(Byers et al., 2021)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal flight

(Iyengar and Wu, 2014)

abnormal mitotic cell cycle | male (with Df(3R)ED6235)

(Lim et al., 2015)

abnormal neurophysiology

(Iyengar and Wu, 2014, Marley and Baines, 2011, Glasscock et al., 2005, Pavlidis and Tanouye, 1995)

abnormal neurophysiology | adult stage

(Saras and Tanouye, 2016, Tan et al., 2004)

bang sensitive

(Horne et al., 2017, Saras and Tanouye, 2016, Kroll et al., 2015, Parker et al., 2011, Glasscock et al., 2005, Hekmat-Scafe et al., 2005, Tan et al., 2004, Zhang et al., 2002)

bang sensitive (with Df(3R)BSC501)

(Horne et al., 2017)

bang sensitive (with Df(3R)BSC874)

(Horne et al., 2017)

bang sensitive (with jus^f04904)

(Horne et al., 2017)

bang sensitive (with jus^HZ.P1)

(Zhang et al., 2002)

bang sensitive | adult stage | heat sensitive

(Byers et al., 2021)

bang sensitive | dominant

(Glasscock and Tanouye, 2005)

decreased cell number | adult stage

(Lim et al., 2015)

decreased cell number | adult stage (with Df(3R)ED6235)

(Lim et al., 2015)

decreased cell number | second instar larval stage (with Df(3R)ED6235)

(Lim et al., 2015)

decreased cell number | third instar larval stage (with Df(3R)ED6235)

(Lim et al., 2015)

paralytic

(Howlett and Tanouye, 2013)

paralytic | cold sensitive (with Df(3R)BSC501)

(Dean et al., 2018)

paralytic | cold sensitive (with jus^f04904)

(Dean et al., 2018)

paralytic | conditional

(Dean et al., 2018)

paralytic | drug conditional

(Howlett and Tanouye, 2013)

paralytic | recessive | cold sensitive

(Dean et al., 2018)

Phenotype Manifest In

centrosome (with Df(3R)ED6235)

(Lim et al., 2015)

cyst progenitor cell | adult stage

(Lim et al., 2015)

cyst progenitor cell | adult stage (with Df(3R)ED6235)

(Lim et al., 2015)

hub cell | adult stage

(Lim et al., 2015)

hub cell | adult stage (with Df(3R)ED6235)

(Lim et al., 2015)

hub cell | second instar larval stage (with Df(3R)ED6235)

(Lim et al., 2015)

hub cell | third instar larval stage (with Df(3R)ED6235)

(Lim et al., 2015)

larval DA1 motor neuron

(Marley and Baines, 2011)

larval RP2 motor neuron

(Marley and Baines, 2011)

male fusome (with Df(3R)ED6235)

(Lim et al., 2015)

male germline stem cell | adult stage

(Lim et al., 2015)

male germline stem cell | adult stage (with Df(3R)ED6235)

(Lim et al., 2015)

male germline stem cell | second instar larval stage (with Df(3R)ED6235)

(Lim et al., 2015)

male germline stem cell | third instar larval stage (with Df(3R)ED6235)

(Lim et al., 2015)

Detailed Description

Statement

Reference

jus^iso7.8/jus^f04904 and jus^iso7.8/Df(3R)BSC501 transheterozygotes show an abnormal and fully penetrant paralysis phenotype upon exposure to 14[o]C or 10[o]C temperatures, which is associated with a significant increase in recovery time from paralysis upon shift to room temperature, as compared to jus^iso7.8 heterozygous and wild-type controls. jus^iso7.8 homozygotes also show an abnormal and nearly fully penetrant paralysis phenotype upon exposure to 10[o]C, but not 14[o]C, temperatures, which is also associated with a significant increase in recovery time from paralysis upon shift to room temperature, as compared to jus^iso7.8 heterozygous and wild-type controls.

jus^iso7.8 mutants suffer paralysis upon exposure to 10s of strobe light (10-50Hz), whose penetrance is variable dependent on the genetic background, and suffer seizures upon exposure to refrigerated food.

(Dean et al., 2018)

jus^iso7.8 as well as jus^iso7.8/jus^f04904, jus^iso7.8/Df(3R)BSC501 and jus^iso7.8/Df(3R)BSC874 flies are bang sensitive with increasing recovery time.

High frequency electrical stimulation of jus^iso7.8 mutant flies induces primary seizure occuring immediately after the stimulus followed by a secondary 'recovery' seizure around 30 sec later - this is significantly shorter than in the jus^f04904 mutants where the average interval between seizures is about 70 sec, the seizure stimulus threshold however is comparable between the mutants.

(Horne et al., 2017)

sda^iso7.8/sda^iso7.8 flies are bang sensitive and have a low seizure threshold in response to high frequency stimulation (similar at room temperature or after brief heat shock).

(Saras and Tanouye, 2016)

sda^iso7.8/sda^iso7.8 mutants are bang sensitive, and do not exhibit synaptic transmission defects upon stimulation of the GF circuit.

(Kroll et al., 2015)

sda^iso7.8/Df(3R)ED6235 mutant testicular niches display decreased hub cell numbers in L2, L3 and adults (but not L1), but no evidence of hub cell death or transdifferentiation; these mutants also show significantly decreased germline stem cell numbers in L2, L3 and adults (but not L1), and decreased cyst stem cell numbers in adults (but not in L3 stage).

sda^iso7.8/Df(3R)ED6235 mutant male germline stem cells display increased mitotic activity (stage unspecified), a significant increase in misoriented centrosomes (adult), a significant decrease in disintegrating fusomes (stage unspecified), and a decrease in the number of germline stem cells with multiple ring canals (stage unspecified) as compared to wild type.

sda^iso7.8/+ mutant testicular niches display decreased numbers of hub cells, germline stem cells and cyst stem cells as compared with controls. (stage unspecified)

(Lim et al., 2015)

There is a drastic increase in DLM firing frequency in sda^iso7.8 mutants following electroconvulsive seizures (ECS). The temporal characteristics of the ECS-induced wing buzzing shows a gross discrepancy with the DLM firing pattern. Unlike in wild-type, the wing beat frequency in these mutants does not appear to be temporally coupled with the DLM firing episode. Instead, an abrupt termination of wing beats occurs during DLM firing, followed by quiescence prior to a second bout of wing beats when DLM firing is terminated.

(Iyengar and Wu, 2014)

Injection of 25mM valproate saline solution greatly increases the seizure threshold of sda^iso7.8 mutants (i.e. suppresses the seizure phenotype), by approximately 7 times (to above wild-type levels without 25mM valproate injection).

(Howlett and Tanouye, 2013)

sda^iso7.8 larvae exhibit prolonged seizures after electroshock.

sda^iso7.8 adult flies exhibit a significantly longer mean recovery time after electroshock compared to wild-type.

Voltage-clamp recordings of spontaneous rhythmic currents in sda^iso7.8 mutant third instar larvae motoneurons show a significant increase in both amplitude and duration. Mini synaptic currents do not show a significant difference in amplitude. The frequency of spontaneous rhythmic currents in sda^iso7.8 mutants is significantly reduced, compared to wild-type.

sda^iso7.8 mutant larvae exhibit significantly slower crawling than their wild-type counterparts, reflecting the reduced frequency of motor excitation.

Current-clamp recordings show that the depolarizations observed in sda^iso7.8 mutant aCC/RP2 motoneurons are slightly larger in amplitude and considerably longer in duration than in wild-type motoneurons and result in increased action potential firing.

aCC/RP2 motoneurons in sda^iso7.8 mutants exhibit altered membrane conductances, such that they have an intrinsically reduced membrane excitability.

Feeding sda^iso7.8 mutant larvae with phenytoin ameliorates seizure-like behavior in these larvae. Similar marked reductions are seen in synaptic current duration and spontaneous rhythmic current amplitude, whereas no significant changes are observed in frequency.

Feeding gravid sda^iso7.8 females with phenytoin results in their offspring exhibiting a complete rescue of mean recovery time from electroshock, relative to controls. The phenytoin is sufficient to suppress synaptic excitation of aCC/RP2 motoneurons.

(Marley and Baines, 2011)

Mean recovery time from bang sensitive paralysis following a mechanical shock is approximately 38 seconds in mutant flies.

(Parker et al., 2011)

Mutant flies have a reduced seizure threshold (the minimum voltage required to induce seizure activity in the dorsal longitudinal muscles after a high-frequency stimulus) compared to controls.

(Glasscock et al., 2005)

Homozygous flies show 100% bang sensitivity.

(Hekmat-Scafe et al., 2005)

Mutants are bang-sensitive, with a recovery period of 37 +/- 5 seconds and a refractory period of 430 +/- 25 seconds.

Mutant flies show a reduced seizure threshold (of 8.3 +/- 0.4 V) compared to wild type in response to high-frequency electrical stimulation.

(Tan et al., 2004)

sda^iso7.8/+ mutants show a lower seizure threshold in the giant fibre pathway than wild type (30.6 +/- 4.5V in heterozygous females compared to 44.5 +/- 4.4V in wild-type females).

(Zhang et al., 2002)

Bang-sensitive mutant. Flies usually show abnormal spontaneous activity ("seizures") in the dorsal longitudinal muscle (DLM) lasting approximately 0.5-3 seconds after the delivery of an electrical buzz (50-400 msec) to the brain. Stimulation of the giant fibre (GF) usually fails to evoke DLM potentials following the buzz. This failure lasts for 88 +/- 37 seconds. There is a close correlation between the seizure and failure phenotypes; if a seizure occurs, a failure also occurs in greater than 95% of cases, while failures without seizures occurred in approximately 10% of cases. GF evoked responses by the DLM are abnormal during recovery from the buzz. After recovery, there is a refactory period during which a buzz is less effective at inducing seizures and failures.

(Pavlidis and Tanouye, 1995)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

jus^iso7.8 has bang sensitive | adult stage | heat sensitive phenotype, enhanceable by SLO2^CRISPR

(Byers et al., 2021)

jus^iso7.8 has bang sensitive | adult stage | heat sensitive phenotype, enhanceable by SLO2^DN.UAS/Scer\GAL4^ChAT.7.4

(Byers et al., 2021)

jus^iso7.8 has decreased cell number | adult stage phenotype, enhanceable by shg[+]/CadN[+]/CadN^unspecified/shg^unspecified

(Lim et al., 2015)

jus^iso7.8 has bang sensitive | dominant phenotype, enhanceable by cpo[+]/cpo^EG1

(Glasscock and Tanouye, 2005)

jus^iso7.8 has bang sensitive | dominant phenotype, enhanceable by cpo[+]/cpo¹

(Glasscock and Tanouye, 2005)

jus^iso7.8 has bang sensitive | dominant phenotype, enhanceable by cpo[+]/cpo^l2

(Glasscock and Tanouye, 2005)

jus^iso7.8 has bang sensitive phenotype, enhanceable by E(sda)B[+]/E(sda)B^B

(Zhang et al., 2002)

jus^iso7.8 has bang sensitive phenotype, enhanceable by E(sda)C[+]/E(sda)C^C

(Zhang et al., 2002)

jus^iso7.8 has bang sensitive phenotype, enhanceable by E(sda)O[+]/E(sda)O^O

(Zhang et al., 2002)

jus^iso7.8 has bang sensitive phenotype, enhanceable by E(sda)A^A/E(sda)A[+]

(Zhang et al., 2002)

jus^iso7.8 has bang sensitive phenotype, enhanceable by E(sda)D[+]/E(sda)D^D

(Zhang et al., 2002)

jus^iso7.8 has bang sensitive phenotype, enhanceable by kcc^DHS1/E(sda)J[+]

(Zhang et al., 2002)

jus^iso7.8 has bang sensitive phenotype, enhanceable by E(sda)F^F/E(sda)F[+]

(Zhang et al., 2002)

jus^iso7.8 has bang sensitive phenotype, enhanceable by E(sda)G[+]/E(sda)G^G

(Zhang et al., 2002)

jus^iso7.8 has bang sensitive phenotype, enhanceable by E(sda)H^H/E(sda)H[+]

(Zhang et al., 2002)

jus^iso7.8 has bang sensitive phenotype, enhanceable by E(sda)I[+]/E(sda)I^I

(Zhang et al., 2002)

jus^iso7.8 has bang sensitive phenotype, enhanceable by E(sda)L[+]/E(sda)L^L

(Zhang et al., 2002)

jus^iso7.8 has bang sensitive phenotype, enhanceable by E(sda)M[+]/E(sda)M^M

(Zhang et al., 2002)

jus^iso7.8 has bang sensitive phenotype, enhanceable by cpo[+]/cpo^EG1

(Zhang et al., 2002)

Suppressed by

Statement

Reference

jus^iso7.8 has bang sensitive phenotype, suppressible by cac^TS2

(Saras and Tanouye, 2016)

jus^iso7.8 has abnormal neurophysiology | adult stage phenotype, suppressible by cac^TS2

(Saras and Tanouye, 2016)

jus^iso7.8 has bang sensitive phenotype, suppressible by shi¹/shi¹

(Kroll et al., 2015)

jus^iso7.8 has bang sensitive phenotype, suppressible by shi²/shi²

(Kroll et al., 2015)

Df(3R)ED6235/jus^iso7.8 has decreased cell number | adult stage phenotype, suppressible by shg^UAS.cSa/Scer\GAL4^upd1.PU

(Lim et al., 2015)

Df(3R)ED6235/jus^iso7.8 has decreased cell number | adult stage phenotype, suppressible by CadN^UAS.cIa/Scer\GAL4^upd1.PU

(Lim et al., 2015)

Df(3R)ED6235/jus^iso7.8 has decreased cell number | adult stage phenotype, suppressible by shg^UAS.cSa/Scer\GAL4^tj.PU

(Lim et al., 2015)

Df(3R)ED6235/jus^iso7.8 has decreased cell number | adult stage phenotype, suppressible by Scer\GAL4^tj.PU/CadN^UAS.cIa

(Lim et al., 2015)

jus^iso7.8 has bang sensitive phenotype, suppressible by para^JS1/para[+]

(Song and Tanouye, 2007)

jus^iso7.8 has bang sensitive | recessive phenotype, suppressible by Top1^JS

(Song et al., 2007)

jus^iso7.8 has bang sensitive | recessive phenotype, suppressible by Top1¹¹²/Top1^JS

(Song et al., 2007)

jus^iso7.8 has bang sensitive phenotype, suppressible by mei-P26¹⁶/mei-P26¹⁶

(Glasscock et al., 2005)

jus^iso7.8 has bang sensitive phenotype, suppressible by mei-P26^fs1/mei-P26¹⁶

(Glasscock et al., 2005)

jus^iso7.8 has abnormal neurophysiology phenotype, suppressible by mei-P26¹⁶

(Glasscock et al., 2005)

jus^iso7.8 has bang sensitive phenotype, suppressible by esg^EP2009/Scer\GAL4^elav.PLu

(Hekmat-Scafe et al., 2005)

jus^iso7.8 has bang sensitive phenotype, suppressible by esg^EP2159/Scer\GAL4^elav.PLu

(Hekmat-Scafe et al., 2005)

jus^iso7.8 has bang sensitive phenotype, suppressible by esg^EP2408/Scer\GAL4^elav.PLu

(Hekmat-Scafe et al., 2005)

jus^iso7.8 has bang sensitive phenotype, suppressible by esg^EP633/Scer\GAL4^elav.PLu

(Hekmat-Scafe et al., 2005)

jus^iso7.8 has bang sensitive phenotype, suppressible by esg^UAS.cFa/Scer\GAL4^elav.PLu

(Hekmat-Scafe et al., 2005)

jus^iso7.8 has bang sensitive phenotype, suppressible by esg^EP684/Scer\GAL4^elav.PLu

(Hekmat-Scafe et al., 2005)

NOT suppressed by

Statement

Reference

Df(3R)ED6235/jus^iso7.8 has decreased cell number | adult stage phenotype, non-suppressible by shg^UAS.cSa/Scer\GAL4^{VP16.nanos.UTR}

(Lim et al., 2015)

Df(3R)ED6235/jus^iso7.8 has decreased cell number | adult stage phenotype, non-suppressible by Scer\GAL4^{VP16.nanos.UTR}/CadN^UAS.cIa

(Lim et al., 2015)

CG46339^{E-A.UASp.Tag:HA}, Df(3R)ED6235/jus^iso7.8, Scer\GAL4^upd1.PU has decreased cell number | adult stage phenotype, non-suppressible by upd1^UAS.cZa, Scer\GAL4^upd1.PU

(Lim et al., 2015)

jus^iso7.8 has bang sensitive | recessive phenotype, non-suppressible by Top1^G0229/Top1^JS

(Song et al., 2007)

jus^iso7.8 has bang sensitive | recessive phenotype, non-suppressible by Top1^G0201/Top1^JS

(Song et al., 2007)

jus^iso7.8 has bang sensitive | recessive phenotype, non-suppressible by Top1^G0134/Top1^JS

(Song et al., 2007)

jus^iso7.8 has bang sensitive phenotype, non-suppressible by esg^EP683/Scer\GAL4^elav.PLu

(Hekmat-Scafe et al., 2005)

Enhancer of

Statement

Reference

jus^iso7.8/sda[+] is an enhancer of bang sensitive | recessive phenotype of kcc^DHS1

(Hekmat-Scafe et al., 2006)

Other

Statement

Reference

SLO2^DN.UAS, Scer\GAL4^ChAT.7.4, jus^iso7.8 has uncoordinated | adult stage phenotype

(Byers et al., 2021)

SLO2^CRISPR, jus^iso7.8 has uncoordinated | adult stage phenotype

(Byers et al., 2021)

jus^iso7.8, shi¹ has abnormal neurophysiology phenotype

(Kroll et al., 2015)

jus^iso7.8/sda[+], kcc^DHS1 has bang sensitive | dominant phenotype

(Hekmat-Scafe et al., 2006)

cpo¹, jus^iso7.8/sda[+] has abnormal neurophysiology phenotype

(Glasscock and Tanouye, 2005)

cpo[+]/cpo^EG1, jus^iso7.8 has abnormal neurophysiology | dominant phenotype

(Glasscock and Tanouye, 2005)

cpo[+]/cpo¹, jus^iso7.8 has abnormal neurophysiology | dominant phenotype

(Glasscock and Tanouye, 2005)

Phenotype Manifest In

Suppressed by

Statement

Reference

Df(3R)ED6235/jus^iso7.8 has hub cell | adult stage phenotype, suppressible by shg^UAS.cSa/Scer\GAL4^upd1.PU

(Lim et al., 2015)

Df(3R)ED6235/jus^iso7.8 has male germline stem cell | adult stage phenotype, suppressible | partially by shg^UAS.cSa/Scer\GAL4^upd1.PU

(Lim et al., 2015)

Df(3R)ED6235/jus^iso7.8 has cyst progenitor cell | adult stage phenotype, suppressible by shg^UAS.cSa/Scer\GAL4^upd1.PU

(Lim et al., 2015)

Df(3R)ED6235/jus^iso7.8 has hub cell | adult stage phenotype, suppressible by CadN^UAS.cIa/Scer\GAL4^upd1.PU

(Lim et al., 2015)

Df(3R)ED6235/jus^iso7.8 has male germline stem cell | adult stage phenotype, suppressible | partially by CadN^UAS.cIa/Scer\GAL4^upd1.PU

(Lim et al., 2015)

Df(3R)ED6235/jus^iso7.8 has cyst progenitor cell | adult stage phenotype, suppressible by CadN^UAS.cIa/Scer\GAL4^upd1.PU

(Lim et al., 2015)

Df(3R)ED6235/jus^iso7.8 has hub cell | adult stage phenotype, suppressible by shg^UAS.cSa/Scer\GAL4^tj.PU

(Lim et al., 2015)

Df(3R)ED6235/jus^iso7.8 has male germline stem cell | adult stage phenotype, suppressible | partially by shg^UAS.cSa/Scer\GAL4^tj.PU

(Lim et al., 2015)

Df(3R)ED6235/jus^iso7.8 has cyst progenitor cell | adult stage phenotype, suppressible by shg^UAS.cSa/Scer\GAL4^tj.PU

(Lim et al., 2015)

Df(3R)ED6235/jus^iso7.8 has hub cell | adult stage phenotype, suppressible by Scer\GAL4^tj.PU/CadN^UAS.cIa

(Lim et al., 2015)

Df(3R)ED6235/jus^iso7.8 has male germline stem cell | adult stage phenotype, suppressible | partially by Scer\GAL4^tj.PU/CadN^UAS.cIa

(Lim et al., 2015)

Df(3R)ED6235/jus^iso7.8 has cyst progenitor cell | adult stage phenotype, suppressible by Scer\GAL4^tj.PU/CadN^UAS.cIa

(Lim et al., 2015)

NOT suppressed by

Statement

Reference

CG46339^{E-A.UASp.Tag:HA}, Df(3R)ED6235/jus^iso7.8, Scer\GAL4^upd1.PU has hub cell | adult stage phenotype, non-suppressible by upd1^UAS.cZa, Scer\GAL4^upd1.PU

(Lim et al., 2015)

CG46339^{E-A.UASp.Tag:HA}, Df(3R)ED6235/jus^iso7.8, Scer\GAL4^upd1.PU has male germline stem cell | adult stage phenotype, non-suppressible by upd1^UAS.cZa, Scer\GAL4^upd1.PU

(Lim et al., 2015)

CG46339^{E-A.UASp.Tag:HA}, Df(3R)ED6235/jus^iso7.8, Scer\GAL4^upd1.PU has cyst progenitor cell | adult stage phenotype, non-suppressible by upd1^UAS.cZa, Scer\GAL4^upd1.PU

(Lim et al., 2015)

Df(3R)ED6235/jus^iso7.8 has male fusome phenotype, non-suppressible by shg^UAS.cSa/Scer\GAL4^upd1.PU

(Lim et al., 2015)

Df(3R)ED6235/jus^iso7.8 has hub cell | adult stage phenotype, non-suppressible by shg^UAS.cSa/Scer\GAL4^{VP16.nanos.UTR}

(Lim et al., 2015)

Df(3R)ED6235/jus^iso7.8 has male germline stem cell | adult stage phenotype, non-suppressible by shg^UAS.cSa/Scer\GAL4^{VP16.nanos.UTR}

(Lim et al., 2015)

Df(3R)ED6235/jus^iso7.8 has cyst progenitor cell | adult stage phenotype, non-suppressible by shg^UAS.cSa/Scer\GAL4^{VP16.nanos.UTR}

(Lim et al., 2015)

Df(3R)ED6235/jus^iso7.8 has hub cell | adult stage phenotype, non-suppressible by Scer\GAL4^{VP16.nanos.UTR}/CadN^UAS.cIa

(Lim et al., 2015)

Df(3R)ED6235/jus^iso7.8 has male germline stem cell | adult stage phenotype, non-suppressible by Scer\GAL4^{VP16.nanos.UTR}/CadN^UAS.cIa

(Lim et al., 2015)

Df(3R)ED6235/jus^iso7.8 has cyst progenitor cell | adult stage phenotype, non-suppressible by Scer\GAL4^{VP16.nanos.UTR}/CadN^UAS.cIa

(Lim et al., 2015)

Other

Statement

Reference

Df(3R)ED6235/jus^iso7.8, bam^hs.PO has male germline stem cell | adult stage | heat sensitive phenotype

(Lim et al., 2015)

CG46339^UASp.Tag:HA, Df(3R)ED6235/jus^iso7.8, Scer\GAL4^upd1.PU, bam^hs.PO has male germline stem cell | adult stage | heat sensitive phenotype

(Lim et al., 2015)

Additional Comments

Genetic Interactions

Statement

Reference

SLO2^CRISPR, jus^iso7.8 and Scer\GAL4^ChAT.7.4>SLO2^DN.UAS, jus^iso7.8 double mutants exhibit spontaneous seizures.

(Byers et al., 2021)

cac^TS2/Y largely suppresses bang sensitivity (reduces behavioral paralysis) in sda^iso7.8/sda^iso7.8 flies at room temperature; this suppression is increased (total suppression) after brief heat shock. After brief heat shock, cac^TS2/Y suppresses the low seizure threshold in sda^iso7.8/sda^iso7.8 flies, almost restoring it to wild type levels.

(Saras and Tanouye, 2016)

shi¹/shi¹ or shi²/shi² partially rescues the bang sensitive phenotype of sda^iso7.8/sda^iso7.8 mutants both at 23[o]C and when incubated at 26[o]C or 27[o]C before testing.

shi¹/shi¹, sda^iso7.8/sda^iso7.8 double mutants exhibit synaptic transmission defects upon stimulation of the GF circuit, showing a failure to respond to a proportion of 1 Hz stimulation pulses.

(Kroll et al., 2015)

CadN^unspecified/+, shg^unspecified/+; sda^iso7.8/+ mutants display a more severe decrease in numbers of hub cells, germline stem cells and cyst stem cells in the testicular niche as compared with sda^iso7.8/+ mutants.

Expression of either shg^Scer\UAS.cSa or CadN^Scer\UAS.cIa under the control of either Scer\GAL4^upd1.PU or Scer\GAL4^tj.PU fully rescues the decreased cell numbers of hub cells and cyst stem cells, and partially rescues the decreased number of germline stem cells, in the adult testicular niche of sda^iso7.8/Df(3R)ED6235 mutants.

Expression of shg^Scer\UAS.cSa under the control of Scer\GAL4^upd1.PU fails to rescue the decrease in germline stem cells with disintegrating fusomes seen in sda^iso7.8/Df(3R)ED6235 mutants.

Expression of either shg^Scer\UAS.cSa or CadN^Scer\UAS.cIa under the control of Scer\GAL4^{nos.UTR.T:Hsim\VP16} fails to rescue the germline stem cell, cyst stem cell, and hub cell number phenotypes in testicular niches of sda^iso7.8/Df(3R)ED6235 mutants.

Expression of upd1^Scer\UAS.cZa under the control of Scer\GAL4^upd1.PU fails to rescue the germline stem cell, cyst stem cell, and hub cell number phenotypes in testicular niches of flies expressing sda^{E-A.Scer\UAS.P\T.T:Ivir\HA1} under the control of Scer\GAL4^upd1.PU in sda^iso7.8/Df(3R)ED6235 mutants, and has no significant effect on cell numbers in testicular niches of flies expressing sda^{Scer\UAS.P\T.T:Ivir\HA1} under the control of Scer\GAL4^upd1.PU in sda^iso7.8/Df(3R)ED6235 mutants.

sda^iso7.8/Df(3R)ED6235 mutants fail to undergo spermatogonial dedifferentiation and germline stem cell number recovery in the testicular niche after expression of bam^hs.PO via heat shock treatment.

Expression of sda^{Scer\UAS.P\T.T:Ivir\HA1}, but not sda^{ΔCAT.Scer\UAS.P\T.T:Ivir\HA1} or sda^{E-A.Scer\UAS.P\T.T:Ivir\HA1}, under the control of Scer\GAL4^upd1.PU, restores the ability of sda^iso7.8/Df(3R)ED6235 mutants to undergo spermatogonial dedifferentiation and recover germline stem cell number in the testicular niche after expression of bam^hs.PO via heat shock treatment.

(Lim et al., 2015)

para^JS1/+ increases the minimum voltage of high frequency stimulation of the brain required to induce seizure (the seizure threshold) of sda^iso7.8 homozygotes to almost wild-type levels.

(Song and Tanouye, 2007)

The bang sensitive phenotype and decreased seizure threshold of sda^iso7.8 homozygotes is partially suppressed by Top1^JS/Y or Top1^JS/Top1¹¹², but not by Top1^JS/Top1^G0229, Top1^JS/Top1^G0201 or Top1^JS/Top1^G0134.

(Song et al., 2007)

Unlike either heterozygote alone, a bang sensitive phenotype is seen in kcc^DHS1/+; sda^iso7.8/+ adults (penetrance 51%). The penetrance of this phenotype rises to 82% in kcc^DHS1/kcc^DHS1; sda^iso7.8/+ adults.

(Hekmat-Scafe et al., 2006)

cpo¹ acts as a dominant enhancer of seizure susceptibility; the cpo¹/+ sda^iso7.8/+ double heterozygote has a seizure threshold of 11.8 +/- 1.6 V. cpo^EG1 acts as a dominant enhancer of seizure susceptibility; the cpo^EG1/+ sda^iso7.8/+ double heterozygote has a seizure threshold of 14.4 +/- 3.1 V.

(Glasscock and Tanouye, 2005)

The reduced seizure threshold (the minimum voltage required to induce seizure activity in the dorsal longitudinal muscles after a high-frequency stimulus) of sda^iso7.8 flies is restored to wild-type if the flies are also mutant for mei-P26¹⁶.

(Glasscock et al., 2005)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Fails to complement

jus^HZ.P1

(Zhang et al., 2002)

Rescued by

jus^iso7.8 is rescued by Scer\GAL4^elav-C155/jus^UAS.cHa

(Horne et al., 2017)

Df(3R)ED6235/jus^iso7.8 is rescued by CG46339^UASp.Tag:HA/Scer\GAL4^upd1.PU

(Lim et al., 2015)

Partially rescued by

jus^f04904/jus^iso7.8 is partially rescued by jus^{Tag:TY1,sfGFP,Tag:V5,Tag:BLRP,Tag:FLAG}

(Horne et al., 2017)

jus^iso7.8 is partially rescued by Scer\GAL4^GMR55G02/jus^UAS.cHa

(Horne et al., 2017)

Df(3R)ED6235/jus^iso7.8 is partially rescued by Scer\GAL4^C587/CG46339^UASp.Tag:HA

(Lim et al., 2015)

Df(3R)ED6235/jus^iso7.8 is partially rescued by Scer\GAL4^{VP16.nanos.UTR}/CG46339^UASp.Tag:HA

(Lim et al., 2015)

Not rescued by

Df(3R)ED6235/jus^iso7.8 is not rescued by CG46339^{E-A.UASp.Tag:HA}/Scer\GAL4^upd1.PU

(Lim et al., 2015)

Df(3R)ED6235/jus^iso7.8 is not rescued by CG46339^{ΔCAT.UASp.Tag:HA}/Scer\GAL4^upd1.PU

(Lim et al., 2015)

Comments

The bang-sensitive phenotype of jus^iso7.8 mutant flies is fully rescued by expression of jus^Scer\UAS.cHa under the control of Scer\GAL4^elav-C155 in the mutant background and partially so when expressed under the Scer\GAL4^GMR55G02 driver.

The median recovery time after mechanical shock-induced paralysis of jus^iso7.8/jus^f04904 flies is significantly shortened by combination with a single copy of jus^{T:Scer\TY1,T:Avic\GFP-SF,T:SV5\V5,T:Zzzz\BLRP,T:Zzzz\FLAG}.

The bang-sensitivity phenotype of jus^iso7.8 mutants cannot be complemented by combination with any of the following: Df(3R)ED6310, Df(3R)BSC874, Df(3R)BSC501 or jus^f04904, is largely complemented by combination with jus^MI11213 (only 7.5% of the transheterozygous animals are bang-sensitive) and fully complemented by any of the following: Df(3R)ED6255, jus^MB12140, CG11898^e03595, α-Man-Ib^f07221, yem^EY23024, CG14516^HP31652, Atg14^EY14568, eIF2D^f04182, CG11897^KG04612, Ctl2^EY00989.

(Horne et al., 2017)

Expression of sda^{Scer\UAS.P\T.T:Ivir\HA1} under the control of Scer\GAL4^upd1.PU fully rescues the testicular niche phenotypes of sda^iso7.8/Df(3R)ED6235 mutants.

Expression of sda^{Scer\UAS.P\T.T:Ivir\HA1} under the control of Scer\GAL4^C587 almost fully rescues the decreased cell numbers in the testicular niche of sda^iso7.8/Df(3R)ED6235 mutants.

Expression of sda^{Scer\UAS.P\T.T:Ivir\HA1} under the control of Scer\GAL4^{nos.UTR.T:Hsim\VP16} partially rescues the germline stem cell number decrease of sda^iso7.8/Df(3R)ED6235 mutants.

Expression of sda^{ΔCAT.Scer\UAS.P\T.T:Ivir\HA1} or sda^{E-A.Scer\UAS.P\T.T:Ivir\HA1} under the control of Scer\GAL4^upd1.PU fails to rescue the testicular niche phenotypes of sda^iso7.8/Df(3R)ED6235 mutants.

(Lim et al., 2015)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

T. Tully

(Zhang et al., 2002)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (8)

Reported As

Symbol Synonym

CG14509^iso7.8

Slamdance^iso7.8

(Lin et al., 2017)

jus^iso7.8

(Byers et al., 2021)

jus<up>sda iso7.8</up>

(Dean et al., 2018)

sda^is07.8

(Saras and Tanouye, 2016)

sda^iso7.8

(Jeong et al., 2021, Horne et al., 2017, Lim et al., 2015, Iyengar and Wu, 2014, Marley and Baines, 2011, Parker et al., 2011, Song and Tanouye, 2007, Song et al., 2007, Hekmat-Scafe et al., 2006, Tan et al., 2004)

sda^iso70.8

(Kroll et al., 2015)

Name Synonyms

slamdanceiso7.8

(Marley and Baines, 2011)

Secondary FlyBase IDs

References (24)

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