UASp regulatory sequences drive expression of CG46339 containing an E to A amino acid replacement at a residue in the catalytic domain which is critical for substrate recognition (this results in an enzymatically inactive form of the protein). The protein is tagged at the N-terminal end with Tag:HA.
CG46339E-A.UASp.Tag:HA, Df(3R)ED6235/jusiso7.8, Scer\GAL4upd1.PU has decreased cell number | adult stage phenotype, non-suppressible by upd1UAS.cZa, Scer\GAL4upd1.PU
CG46339E-A.UASp.Tag:HA, Df(3R)ED6235/jusiso7.8, Scer\GAL4upd1.PU has hub cell | adult stage phenotype, non-suppressible by upd1UAS.cZa, Scer\GAL4upd1.PU
CG46339E-A.UASp.Tag:HA, Df(3R)ED6235/jusiso7.8, Scer\GAL4upd1.PU has male germline stem cell | adult stage phenotype, non-suppressible by upd1UAS.cZa, Scer\GAL4upd1.PU
CG46339E-A.UASp.Tag:HA, Df(3R)ED6235/jusiso7.8, Scer\GAL4upd1.PU has cyst progenitor cell | adult stage phenotype, non-suppressible by upd1UAS.cZa, Scer\GAL4upd1.PU
Expression of sdaE-A.Scer\UAS.P\T.T:Ivir\HA1 under the control of Scer\GAL4upd1.PU fails to restore the ability of sdaiso7.8/Df(3R)ED6235 mutants to undergo spermatogonial dedifferentiation and recover germline stem cell number in the testicular niche after expression of bamhs.PO via heat shock treatment.
Expression of upd1Scer\UAS.cZa under the control of Scer\GAL4upd1.PU fails to rescue the germline stem cell, cyst stem cell, and hub cell number phenotypes in testicular niches of flies expressing sdaE-A.Scer\UAS.P\T.T:Ivir\HA1 under the control of Scer\GAL4upd1.PU in sdaiso7.8/Df(3R)ED6235 mutants.
CG46339E-A.UASp.Tag:HA/Scer\GAL4upd1.PU fails to rescue Df(3R)ED6235/jusiso7.8
Expression of sdaE-A.Scer\UAS.P\T.T:Ivir\HA1 under the control of Scer\GAL4upd1.PU fails to rescue the testicular niche phenotypes of sdaiso7.8/Df(3R)ED6235 mutants.