FB2024_03 , released June 25, 2024
Allele: Dmel\SLO2CRISPR
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General Information
Symbol
Dmel\SLO2CRISPR
Species
D. melanogaster
Name
FlyBase ID
FBal0370934
Feature type
allele
Associated gene
Dmel\SLO2
Associated Insertion(s)
TI{TI}SLO2CRISPR
Carried in Construct
Key Links
Genomic Maps

Allele class
Mutagen
Nature of the Allele
Allele class
Mutagen
CRISPR/Cas9
(Byers et al., 2021)
gene targeting by homologous recombination
(Byers et al., 2021)
Progenitor genotype
Associated Insertion(s)
TI{TI}SLO2CRISPR
Cytology
Description

Sequence encoding the pore region has been deleted from the endogenous SLO2 locus. A sequence cassette that includes 1. a loxP cassette containing a Disc\RFPDsRed(T1).3xP3.cHa marker, and 2. a single attP site has been inserted in place of the deleted sequence.

(Byers et al., 2021)
Allele components
Component
Use(s)
Also carries
attP
loxP
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
delins
2R:10,338,675..10,338,803 [-]
Comment:

A sequence encoding the pore region of the endogenous SLO2 locus has been deleted. A sequence cassette that includes a loxP cassette containing a Disc\RFPDsRed(T1).3xP3.cHa marker and a single attP site has been inserted in place of the deleted sequence.

(Tsunoda, 2022.2.11)
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 1 )
      Disease
      Interaction
      References
      exacerbates  epilepsy
      modeled by paraGEFS+
      (Byers et al., 2021)
      modeled by jusiso7.8
      (Byers et al., 2021)
      modeled by parabss1
      (Byers et al., 2021)
      modeled by eas2
      (Byers et al., 2021)
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      SLO2CRISPR neurons show no neuronal transmission differences to controls, except with the exception of an increase in the interval between the first and second action potential.

      (Byers et al., 2021)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Enhancer of
      Statement
      Reference

      SLO2CRISPR is an enhancer of bang sensitive | adult stage | heat sensitive phenotype of eas2

      (Byers et al., 2021)

      SLO2CRISPR is an enhancer of bang sensitive | adult stage | heat sensitive phenotype of jusiso7.8

      (Byers et al., 2021)

      SLO2CRISPR is an enhancer of uncoordinated | adult stage | heat sensitive phenotype of paraGEFS+

      (Byers et al., 2021)

      SLO2CRISPR is an enhancer of bang sensitive | adult stage | heat sensitive phenotype of parabss1

      (Byers et al., 2021)
      Other
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      SLO2CRISPR, jusiso7.8 and SLO2CRISPR, parabss1/+ double mutants exhibit spontaneous seizures.

      (Byers et al., 2021)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (1)
      Reported As
      Symbol Synonym
      SLO2CRISPR
      Name Synonyms
      Secondary FlyBase IDs
        References (2)