Expression of sdaScer\UAS.P\T.T:Ivir\HA1 under the control of Scer\GAL4upd1.PU results in a significant increase in the number of male germline stem cells that have disintegrating fusome remnants compared with controls (adult stage), but does not result in an increase in germline stem cell number, as compared to wild type.
CG46339UASp.Tag:HA, Df(3R)ED6235/jusiso7.8, Scer\GAL4upd1.PU, bamhs.PO has male germline stem cell | adult stage | heat sensitive phenotype
Expression of sdaScer\UAS.P\T.T:Ivir\HA1 under the control of Scer\GAL4upd1.PU restores the ability of sdaiso7.8/Df(3R)ED6235 mutants to undergo spermatogonial dedifferentiation and recover germline stem cell number in the testicular niche after expression of bamhs.PO via heat shock treatment.
Expression of upd1Scer\UAS.cZa under the control of Scer\GAL4upd1.PU has no significant effect on cell numbers in testicular niches of flies expressing sdaScer\UAS.P\T.T:Ivir\HA1 under the control of Scer\GAL4upd1.PU in sdaiso7.8/Df(3R)ED6235 mutants.
Scer\GAL4C587/CG46339UASp.Tag:HA partially rescues Df(3R)ED6235/jusiso7.8
Scer\GAL4VP16.nanos.UTR/CG46339UASp.Tag:HA partially rescues Df(3R)ED6235/jusiso7.8
Expression of sdaScer\UAS.P\T.T:Ivir\HA1 under the control of Scer\GAL4upd1.PU fully rescues the decreased cell numbers in the testicular niche of sdaiso7.8/Df(3R)ED6235 mutants.
Expression of sdaScer\UAS.P\T.T:Ivir\HA1 under the control of Scer\GAL4C587 almost fully rescues the decreased cell numbers in the testicular niche of sdaiso7.8/Df(3R)ED6235 mutants.
Expression of sdaScer\UAS.P\T.T:Ivir\HA1 under the control of Scer\GAL4nos.UTR.T:Hsim\VP16 partially rescues the germline stem cell number decrease of sdaiso7.8/Df(3R)ED6235 mutants.