FlyBase Allele Report: Dmel\esg[EP2009]

General Information

Symbol

Dmel\esg^EP2009

Species

D. melanogaster

Name

FlyBase ID

FBal0089289

Feature type

allele

Associated gene

Dmel\esg

Associated Insertion(s)

P{EP}esg^EP2009

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

P-element activity

Nature of the Allele

Allele class

Mutagen

P-element activity

(Rorth et al., 1998)

Progenitor genotype

Associated Insertion(s)

P{EP}esg^EP2009

Cytology

Description

P{EP} insertion 334bp upstream of the esg translation initiation codon. The P{EP} element is oriented such that expression from the element results in overexpression of esg.

(Bidet et al., 2003)

P{EP} insertion at +673bp of the transcription unit.

(Abdelilah-Seyfried et al., 2000)

Allele components

Component

Use(s)

Inserted element

P{EP}

misexpression element

Regulatory region(s)

UAS

binary expression system - regulatory region

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

transposable_element_insertion_site

2L:15,333,757..15,333,757 [+]

(Kim et al., 2010, Hekmat-Scafe et al., 2005, Bidet et al., 2003, Pena-Rangel et al., 2002, Tseng and Hariharan, 2002, Gene Disruption Project members, 2001-, Abdelilah-Seyfried et al., 2000, Ashburner et al., 1999, Ashburner et al., 1999.10.12, Rorth et al., 1998)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neurophysiology, with Scer\GAL4^elav.PLu

(Hekmat-Scafe et al., 2005)

female sterile | recessive

(Ashburner et al., 1999)

lethal, with Scer\GAL4^hs.2sev

(Tseng and Hariharan, 2002)

visible, with Scer\GAL4^en-e16E

(Tseng and Hariharan, 2002)

visible, with Scer\GAL4^ey.PH

(Tseng and Hariharan, 2002)

visible, with Scer\GAL4^sca-537.4

(Abdelilah-Seyfried et al., 2000)

visible | dominant, with Scer\GAL4^bi-omb-Gal4

(Rorth et al., 1998)

Phenotype Manifest In

eye, with Scer\GAL4^ey.PH

(Tseng and Hariharan, 2002)

macrochaeta & scutum, with Scer\GAL4^sca-537.4

(Abdelilah-Seyfried et al., 2000)

microchaeta & scutum, with Scer\GAL4^sca-537.4

(Abdelilah-Seyfried et al., 2000)

wing, with Scer\GAL4^bi-omb-Gal4

(Rorth et al., 1998)

wing, with Scer\GAL4^en-e16E

(Tseng and Hariharan, 2002)

Detailed Description

Statement

Reference

Expression of esg^EP2009 under the control of Scer\GAL4^elav.PLu in a wild-type background raises the mean seizure threshold to 55.5 +/- 13.2 V compared to 36.7 +/- 6.5 V for control flies (for a high frequency stimulus of 0.5msec pulses delivered at 200Hz for 300 msec).

(Hekmat-Scafe et al., 2005)

Expression of esg^EP2009 under the control of Scer\GAL4^ey.PH results in eyes that are strongly reduced in size. Flies expressing esg^EP2009 under the control of Scer\GAL4^en-e16E have scalloped wings.

(Tseng and Hariharan, 2002)

Expression of esg^EP2009 under the control of Scer\GAL4^sca-537.4 results in complete loss of all macrochaetae and microchaetae on the notum.

(Abdelilah-Seyfried et al., 2000)

Expression of esg^EP2009 under the control of Scer\GAL4^bi-omb-Gal4 causes a reduction in the size of the wing.

(Rorth et al., 1998)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT suppressed by

Statement

Reference

Scer\GAL4^ey.PH, esg^EP2009 has visible phenotype, non-suppressible by CycE^UAS.cLa, Scer\GAL4^ey.PH

(Tseng and Hariharan, 2002)

Enhancer of

Statement

Reference

esg^EP2009 is an enhancer of visible phenotype of Dcp-1^UAS.cKa, Scer\GAL4^GMR.PF

(Kim et al., 2010)

Suppressor of

Statement

Reference

esg^EP2009/Scer\GAL4^elav.PLu is a suppressor of bang sensitive phenotype of para^bss1

(Hekmat-Scafe et al., 2005)

esg^EP2009/Scer\GAL4^elav.PLu is a suppressor of bang sensitive phenotype of eas²

(Hekmat-Scafe et al., 2005)

Scer\GAL4^{elav.Switch.PO}/esg^EP2009 is a suppressor of bang sensitive phenotype of eas²

(Hekmat-Scafe et al., 2005)

esg^EP2009/Scer\GAL4^elav.PLu is a suppressor of abnormal neurophysiology phenotype of eas²

(Hekmat-Scafe et al., 2005)

esg^EP2009/Scer\GAL4^elav.PLu is a suppressor of bang sensitive phenotype of jus^iso7.8

(Hekmat-Scafe et al., 2005)

Other

Statement

Reference

Scer\GAL4^pnr-MD237, esg^EP2009, pnr^MD237/pnr[+] has lethal | embryonic stage phenotype

(Pena-Rangel et al., 2002)

Scer\GAL4^pnr-MD237, esg^EP2009, pnr^MD237 has lethal | embryonic stage phenotype

(Pena-Rangel et al., 2002)

Phenotype Manifest In

NOT suppressed by

Statement

Reference

Scer\GAL4^ey.PH, esg^EP2009 has eye phenotype, non-suppressible by CycE^UAS.cLa, Scer\GAL4^ey.PH

(Tseng and Hariharan, 2002)

Enhancer of

Statement

Reference

esg^EP2009 is an enhancer of eye phenotype of Dcp-1^UAS.cKa, Scer\GAL4^GMR.PF

(Kim et al., 2010)

Additional Comments

Genetic Interactions

Statement

Reference

esg^EP2009 enhances the rough eye phenotype seen when Dcp-1^Scer\UAS.cKa (insertion line P{UAS-Dcp-1.K}19-2) is expressed under the control of Scer\GAL4^GMR.PF.

(Kim et al., 2010)

The bang sensitivity of bss¹/+ flies (more than 50% of flies are bang sensitive) is substantially suppressed by the expression of esg^EP2009 under the control of Scer\GAL4^elav.PLu. However, the 100% bang sensitivity of bss¹/bss¹ flies is not suppressed by the expression of esg^EP2009 under the control of Scer\GAL4^elav.PLu. The suppression of the bang sensitivity of eas² flies that is seen when esg^EP2009 is overexpressed depends on the overexpression occurring in the larval stage. RU486 has been used to drive expression of esg^EP2009 under the control of Scer\GAL4^{elav.Switch.PO} at different time points in development (the Scer\GAL4^{elav.Switch.PO} driver only produces active protein in the presence of RU486) in eas² animals and the bang sensitivity of the resulting adults has been assayed; a reduced level of bang sensitivity (compared to eas² single mutant flies) is seen if RU486 induction occurs during embryonic and larval stages or during larval and adult stages, whereas little reduction in bang sensitivity is seen if RU486 induction occurs only in the adult stage, and only a slight reduction in bang sensitivity is seen if RU486 induction occurs only in the embryonic stage. The seizure sensitivity of eas² flies is partially suppressed by expression of esg^EP2009 under the control of Scer\GAL4^elav.PLu; the mean seizure threshold is raised to 15.5 +/- 6.8 V (for a high frequency stimulus of 0.5msec pulses delivered at 200Hz for 300 msec).

(Hekmat-Scafe et al., 2005)

Co-expression of CycE^Scer\UAS.cLa does not suppress the small eye phenotype caused by expression of esg^EP2009 under the control of Scer\GAL4^ey.PH.

(Tseng and Hariharan, 2002)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

Selected as: a P{EP} insertion line that modifies the pnr^MD237/+ phenotype when expressed using Scer\GAL4^pnr-MD237.

(Pena-Rangel et al., 2002)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

EP(2)2009

(Abdelilah-Seyfried et al., 2000, Ashburner et al., 1999.10.12)

esg^EP2009

Name Synonyms

Secondary FlyBase IDs

References (10)

Report Sections

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