FlyBase Allele Report: Dmel\rl[10a]

General Information

Symbol

Dmel\rl^10a

Species

D. melanogaster

Name

FlyBase ID

FBal0035508

Feature type

allele

Associated gene

Dmel\rl

Associated Insertion(s)

Carried in Construct

Also Known As

rolled^10A

Key Links

Allele class

Mutagen

Nature of the Allele

Allele class

Mutagen

Progenitor genotype

Caused by aberration

Df(2R)rl10a

Cytology

Description

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal mitotic cell cycle | recessive

(Inoue and Glover, 1998)

abnormal wound healing

(Geiger et al., 2011)

decreased occurrence of cell division | larval stage (with rl¹⁰)

(Inoue and Glover, 1998)

lethal | recessive

(Inoue and Glover, 1998)

lethal - all die before end of larval stage (with rl^4H6)

(Biggs et al., 1994)

some die during larval stage (with rl^4H6)

(Biggs et al., 1994)

Phenotype Manifest In

bract (with rl¹)

(del Alamo et al., 2002)

neuroblast | larval stage (with rl¹⁰)

(Inoue and Glover, 1998)

tracheal branch primordium

(Cela and Llimargas, 2006)

Detailed Description

Statement

Reference

Homozygous embryos show wound closure defects.

(Geiger et al., 2011)

Bouton number at the neuromuscular junction is not significantly altered in heterozygous larvae compared to controls.

(Fischer et al., 2009)

rl^10a embryos show branch patterning defects and defects in branch continuity.

(Cela and Llimargas, 2006)

rl^10a/+ flies show a wild-type number of rhabdomeres in their eyes.

(Kim et al., 2006)

rl^10a/+ animals show no significant alteration in bouton number compared to wild-type.

(Koh et al., 2002)

rl¹/rl^10a legs show a complete lack of bracts.

(del Alamo et al., 2002)

rl¹⁰/rl^10a heterozygotes die at the late third instar to pupal stage, earlier than rl⁹/rl^10a which die in the pupal stage. Mitotic index of rl¹⁰/rl^10a larval neuroblasts is clearly reduced compared to wild type. The reduction is typical of a delay in passing through interphase, as a consequence of a reduced ability to transduce proliferation signals. The ratio of metaphase to anaphase figures is elevated, suggesting the cells are being delayed at metaphase. There is a low but significant level of hypercondensed chromosomes in both anaphase and metaphase. Some anaphase defects can be seen including a low frequency of overcondensed chromatids at late anaphase, anaphase figures with lagging chromatids and randomly oriented chromatids at anaphase. In contrast to wild type, there is very little increase in the mitotic index in either rl⁹/rl^10a or rl¹⁰/rl^10a mutants after colchicine treatment for 3 hrs, and over-condensation of the chromosomes is not seen.

(Inoue and Glover, 1998)

Phenotypically neutral with no photoreceptor defects.

(Kockel et al., 1997)

Mutation has no effect on rho^hs.sev rough eye phenotype.

(Freeman, 1994)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhancer of

Statement

Reference

rl[+]/rl^10a is an enhancer of visible phenotype of S6kII^UAS.Tag:MYC, Scer\GAL4^GMR.PF

(Kim et al., 2006)

NOT Enhancer of

Statement

Reference

rl^10a is a non-enhancer of visible phenotype of Scer\GAL4^GMR.PF, btl^λ.UAS, stumps^UAS.Tag:FLAG

(Zhu et al., 2005)

Suppressor of

Statement

Reference

rl[+]/rl^10a is a suppressor | partially of abnormal size | pupal stage phenotype of Scer\GAL4^elav-C155, jeb^UAS.cVa

(Wolfstetter et al., 2017)

rl^10a is a suppressor of decreased cell death phenotype of Ras85D^R68Q

(Gafuik and Steller, 2011)

rl[+]/rl^10a is a suppressor | partially of abnormal neuroanatomy | larval stage phenotype of S6kII^ign-Δ58-1

(Fischer et al., 2009)

rl[+]/rl^10a is a suppressor of abnormal size | larval stage phenotype of Scer\GAL4^en-e16E, msk^UAS.cLa

(Marenda et al., 2006)

rl^10a is a suppressor of increased cell death phenotype of Scer\GAL4^en-e16E, msk^UAS.cLa

(Marenda et al., 2006)

rl[+]/rl^10a is a suppressor of visible phenotype of Scer\GAL4^Tub.PU, csw^N308D.UASp

(Oishi et al., 2006)

rl[+]/rl^10a is a suppressor of visible phenotype of Mkp3¹/Mkp3²

(Rintelen et al., 2003)

rl[+]/rl^10a is a suppressor of abnormal neuroanatomy | dominant phenotype of Fas2^e76

(Koh et al., 2002)

NOT Suppressor of

Statement

Reference

rl[+]/rl^10a is a non-suppressor of lethal phenotype of Hsap\HTT^Q93.ex1.UAS, Scer\GAL4^elav-C155

(Maher et al., 2011)

rl^10a is a non-suppressor of visible phenotype of Scer\GAL4^GMR.PF, btl^λ.UAS, stumps^UAS.Tag:FLAG

(Zhu et al., 2005)

Other

Statement

Reference

Mkp3¹/Mkp3^5J4, PTP-ER^XE-3022, rl^10a has viable phenotype

(Rintelen et al., 2003)

Phenotype Manifest In

Suppressed by

Statement

Reference

rl^10a has phenotype, suppressible by Jra^{S82A.T92A.T107A.hs.sev}

(Peverali et al., 1996)

Enhancer of

Statement

Reference

rl[+]/rl^10a is an enhancer of ganglionic tracheal branch primordium phenotype of Mkp3^GS801, Scer\GAL4^btl.PS

(Cela and Llimargas, 2006)

rl[+]/rl^10a is an enhancer of dorsal tracheal branch primordium phenotype of Mkp3^GS801, Scer\GAL4^btl.PS

(Cela and Llimargas, 2006)

rl[+]/rl^10a is an enhancer of tracheal branch primordium phenotype of Mkp3^GS801, Scer\GAL4^btl.PS

(Cela and Llimargas, 2006)

rl[+]/rl^10a is an enhancer of eye photoreceptor cell phenotype of S6kII^{K231R.UAS.Tag:MYC}, Scer\GAL4^hs.2sev

(Kim et al., 2006)

rl[+]/rl^10a is an enhancer of ommatidium phenotype of S6kII^UAS.Tag:MYC, Scer\GAL4^GMR.PF

(Kim et al., 2006)

rl[+]/rl^10a is an enhancer of interommatidial bristle phenotype of S6kII^UAS.Tag:MYC, Scer\GAL4^GMR.PF

(Kim et al., 2006)

rl[+]/rl^10a is an enhancer of rhabdomere phenotype of S6kII^UAS.Tag:MYC, Scer\GAL4^GMR.PF

(Kim et al., 2006)

rl[+]/rl^10a is an enhancer of eye phenotype of Mkp3^hs.sev

(Rintelen et al., 2003)

rl[+]/rl^10a is an enhancer of embryo | posterior phenotype of Dsor1^Su1, fs(1)M3¹⁹⁰¹

(Lim et al., 1999)

rl[+]/rl^10a is an enhancer of embryonic abdominal segment 8 phenotype of Dsor1^Su1, fs(1)M3¹⁹⁰¹

(Lim et al., 1999)

rl^10a is an enhancer of eye phenotype of hid^GMR.PG

(Bergmann et al., 1998)

rl^10a is an enhancer of ommatidium phenotype of hid^GMR.PG

(Bergmann et al., 1998)

NOT Enhancer of

Statement

Reference

rl^10a is a non-enhancer of eye phenotype of Scer\GAL4^GMR.PF, btl^λ.UAS, stumps^UAS.Tag:FLAG

(Zhu et al., 2005)

Suppressor of

Statement

Reference

rl^10a is a suppressor of eye phenotype of Rap1^V12.hs2sev

(Mavromatakis and Tomlinson, 2012)

rl^10a is a suppressor of photoreceptor cell R7 phenotype of Rap1^V12.hs2sev

(Mavromatakis and Tomlinson, 2012)

rl^10a is a suppressor of ommatidium phenotype of Rap1^V12.hs2sev

(Mavromatakis and Tomlinson, 2012)

rl^10a is a suppressor of rhabdomere | increased number phenotype of Rap1^V12.hs2sev

(Mavromatakis and Tomlinson, 2012)

rl^10a is a suppressor of photoreceptor cell R7 phenotype of Ras85D^R68Q

(Gafuik and Steller, 2011)

rl^10a is a suppressor of wing vein | ectopic phenotype of Ras85D^R68Q

(Gafuik and Steller, 2011)

rl^10a is a suppressor of eye phenotype of Ras85D^R68Q

(Gafuik and Steller, 2011)

rl^10a is a suppressor of midline glial cell phenotype of Ras85D^R68Q

(Gafuik and Steller, 2011)

rl^10a is a suppressor of ommatidium phenotype of Ras85D^R68Q

(Gafuik and Steller, 2011)

rl^10a is a suppressor of wing phenotype of Ras85D^R68Q

(Gafuik and Steller, 2011)

rl^10a is a suppressor of posterior crossvein phenotype of Ras85D^R68Q

(Gafuik and Steller, 2011)

rl^10a is a suppressor of crossvein phenotype of Ras85D^R68Q

(Gafuik and Steller, 2011)

rl^10a is a suppressor of wing vein L4 phenotype of Ras85D^R68Q

(Gafuik and Steller, 2011)

rl^10a is a suppressor of wing vein L5 phenotype of Ras85D^R68Q

(Gafuik and Steller, 2011)

rl[+]/rl^10a is a suppressor of eye | somatic clone phenotype of gfr¹

(Beam and Moberg, 2010)

rl[+]/rl^10a is a suppressor | partially of NMJ bouton | larval stage phenotype of S6kII^ign-Δ58-1

(Fischer et al., 2009)

rl^10a/Scer\GAL4^Tub.PU is a suppressor of wing vein phenotype of Scer\GAL4^Tub.PU, csw^Y279C.UASp

(Oishi et al., 2009)

rl[+]/rl^10a is a suppressor of wing disc posterior compartment | larval stage phenotype of Scer\GAL4^en-e16E, msk^UAS.cLa

(Marenda et al., 2006)

Scer\GAL4^en-e16E/rl^10a is a suppressor of wing phenotype of msk^UAS.cLa

(Marenda et al., 2006)

rl^10a is a suppressor of anterior crossvein phenotype of Scer\GAL4^en-e16E, msk^UAS.cLa

(Marenda et al., 2006)

rl^10a is a suppressor of posterior crossvein phenotype of Scer\GAL4^en-e16E, msk^UAS.cLa

(Marenda et al., 2006)

rl^10a is a suppressor of wing vein L4 phenotype of Scer\GAL4^en-e16E, msk^UAS.cLa

(Marenda et al., 2006)

rl[+]/rl^10a is a suppressor of wing vein | ectopic phenotype of Scer\GAL4^Tub.PU, csw^N308D.UASp

(Oishi et al., 2006)

rl[+]/rl^10a is a suppressor of eye photoreceptor cell phenotype of Mkp3¹/Mkp3²

(Rintelen et al., 2003)

rl[+]/rl^10a is a suppressor of wing vein | increased number phenotype of Mkp3¹/Mkp3²

(Rintelen et al., 2003)

rl[+]/rl^10a is a suppressor of bouton | increased number phenotype of Fas2^e76

(Koh et al., 2002)

rl^10a is a suppressor of phenotype of Ras85D^V12.sev

(Peverali et al., 1996)

rl^10a is a suppressor of phenotype of svp^2.sev

(Begemann et al., 1995)

rl^10a is a suppressor of phenotype of svp^1.sev

(Begemann et al., 1995)

NOT Suppressor of

Statement

Reference

rl[+]/rl^10a is a non-suppressor of eye photoreceptor cell phenotype of Hsap\HTT^Q93.ex1.UAS, Scer\GAL4^elav-C155

(Maher et al., 2011)

rl^10a is a non-suppressor of eye phenotype of Scer\GAL4^GMR.PF, btl^λ.UAS, stumps^UAS.Tag:FLAG

(Zhu et al., 2005)

Additional Comments

Genetic Interactions

Statement

Reference

The reduced pupal size characteristic for animals expressing jeb^Scer\UAS.cVa under the control of Scer\GAL4^elav-C155 is partially suppressed by combination with a single copy of rl^10a.

(Wolfstetter et al., 2017)

The eye roughening and multiple R7 phenotype of R^V12.hs2sev mutants is significantly suppressed by the loss of one copy of rl (in rl^10a heterozygotes).

(Mavromatakis and Tomlinson, 2012)

The enlarged eye phenotype seen in mosaic animals in which the eye is homozygous for gfr¹ is suppressed by rl^10a/+.

(Beam and Moberg, 2010)

The increase in bouton number per muscle area that is seen at the neuromuscular junction (NMJ) in S6kII^ign-Δ58-1 larvae is significantly suppressed by rl^10a/+, although the increase in absolute bouton number that is seen at the NMJ in S6kII^ign-Δ58-1 larvae is not suppressed by rl^10a/+.

(Fischer et al., 2009)

The presence of a rl^10a background significantly suppresses ectopic wing vein formation found in csw^{Y279C.Scer\UAS} (Scer\GAL4^tub) mutants.

(Oishi et al., 2009)

The branch integrity defects of Scer\GAL4^btl.PS>Mkp3^GS801 embryos are increased when these embryos have a rl^10a/+ background relative to a wild-type background.

(Cela and Llimargas, 2006)

Expression of S6kII^{Scer\UAS.T:Hsap\MYC}, under the control of Scer\GAL4^GMR.PF, causes a more severe roughened-eye phenotype and a greater loss of rhabdomeres when expressed in a rl^10a/+ background than when expressed in a wild-type background.

Expression of S6kII^{K231R.Scer\UAS.T:Hsap\MYC}, under the control of

Scer\GAL4^hs.2sev, causes a more severe loss of photoreceptor cells when expressed or rl^10a/+ background.

(Kim et al., 2006)

Reduction of EGFR pathway function in rl^10a heterozygotes strongly suppresses the msk^Scer\UAS.cLa Scer\GAL4^en-e16E `Twiggy wing' (exhibiting a nearly normal anterior compartment and severely reduced posterior compartments) phenotype.

(Marenda et al., 2006)

The Mkp3^hs.sev rough eye phenotype is enhanced by rl^10a/+.

(Rintelen et al., 2003)

The increase in bouton number observed in Fas2^e76/+ heterozygotes is partially suppressed by rl^10a/+ mutants.

(Koh et al., 2002)

Embryos derived from homozygous fs(1)M3¹⁹⁰¹ females fail to develop structures posterior to the seventh abdominal segment as well as anterior-most structures, including the head skeleton. The posterior defect is significantly suppressed if the female is also carrying one copy of Dsor1^Su1; an eighth abdominal segment is formed. This suppression is significantly reduced if the females are also carrying one copy of rl^10a.

(Lim et al., 1999)

Suppressor of the dosage-dependent (two or more copies of P{sev-svp1} or P{sev-svp2}) transformation of cone cells into R7 photoreceptors and at a lower frequency R7 cells into outer photoreceptors.

(Begemann et al., 1995)

Xenogenetic Interactions

Statement

Reference

The neuronal degeneration phenotype and decreased survival of animals expressing Hsap\HD^{Q93.ex1p.Scer\UAS} under the control of Scer\GAL4^elav-C155 is not suppressed in a rl^10a/+ mutant background.

(Maher et al., 2011)

Complementation and Rescue Data

Rescued by

rl^4H6/rl^10a is rescued by rl^hs.PB

(Biggs et al., 1994)

Not rescued by

rl^4H6/rl^10a is not rescued by rl^K164.hs

(Biggs et al., 1994)

Comments

Images (0)

Mutant

Wild-type

Stocks (2)

Bloomington

742

Df(2R)rl10a, lt¹ rl^10a cn¹/In(2LR)bw^V1, ds^33k bw^V1

Kyoto

106058

Df(2R)rl10a, lt¹ rl^10a cn¹/In(2LR)bw^V1, ds^33k bw^V1

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (5)

Reported As

Symbol Synonym

ERK/rl^10a

(Geiger et al., 2011)

rl^10A

(Oishi et al., 2009, Marenda et al., 2006, Oishi et al., 2006, Kockel et al., 1997)

rl^10a

(Kim et al., 2023, Mangione et al., 2023, Wolfstetter et al., 2017, Gafuik and Steller, 2011, Beam and Moberg, 2010, Fischer et al., 2009, Cela and Llimargas, 2006, Kim et al., 2006)

rolled^10A

(del Alamo et al., 2002)

Name Synonyms

rolled10a

(Maher et al., 2011)

Secondary FlyBase IDs

References (29)

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