FlyBase Allele Report: Dmel\S6kII[K231R.UAS.Tag:MYC]

General Information

Symbol

Dmel\S6kII^{K231R.UAS.Tag:MYC}

Species

D. melanogaster

Name

FlyBase ID

FBal0194034

Feature type

allele

Associated gene

Dmel\S6kII

Associated Insertion(s)

Carried in Construct

P{UAS-RSK.K231R}

Key Links

Transgenic product class

missense_variant

(Kim et al., 2006)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

missense_variant

(Kim et al., 2006)

Mutagen

in vitro construct

(Kim et al., 2006)

Progenitor genotype

Carried in construct

P{UAS-RSK.K231R}

Cytology

Description

Amino acid replacement: K231R.

(Kim et al., 2006)

UASt regulatory sequences drive expression of a kinase-dead form of S6kII, which is tagged with Tag:MYC.

(Kim et al., 2006)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

S6kII

Tagged with

Tag:MYC

epitope tag

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

eye photoreceptor cell, with Scer\GAL4^hs.2sev

(Kim et al., 2006)

retina, with Scer\GAL4^GMR.PF

(Kim et al., 2006)

wing vein, with Scer\GAL4^en-e16E

(Kim et al., 2006)

Detailed Description

Statement

Reference

Expression of S6kII^{K231R.Scer\UAS.T:Hsap\MYC} in the eye, under the control of Scer\GAL4^GMR.PF, disrupts the differentiation of retinal cells.

Expression of S6kII^{K231R.Scer\UAS.T:Hsap\MYC} under the control of Scer\GAL4^en-e16E disrupts the formation of wing veins.

Expression of S6kII^{K231R.Scer\UAS.T:Hsap\MYC} under the control of Scer\GAL4^hs.2sev leads to a smaller number of photoreceptor cells per ommatidium in the eye.

(Kim et al., 2006)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressor of

Statement

Reference

S6kII^{K231R.UAS.Tag:MYC}, Scer\GAL4^hs.2sev is a suppressor of visible phenotype of Ras85D^V12.UAS, Scer\GAL4^hs.2sev

(Kim et al., 2006)

S6kII^{K231R.UAS.Tag:MYC}, Scer\GAL4^hs.2sev is a suppressor of visible phenotype of Raf^UAS.F179, Scer\GAL4^hs.2sev

(Kim et al., 2006)

Phenotype Manifest In

Enhanced by

Statement

Reference

S6kII^{K231R.UAS.Tag:MYC}, Scer\GAL4^hs.2sev has eye photoreceptor cell phenotype, enhanceable by Ras85D^e1B/Ras85D[+]

(Kim et al., 2006)

S6kII^{K231R.UAS.Tag:MYC}, Scer\GAL4^hs.2sev has eye photoreceptor cell phenotype, enhanceable by rl[+]/rl^10a

(Kim et al., 2006)

Suppressor of

Statement

Reference

S6kII^{K231R.UAS.Tag:MYC}, Scer\GAL4^hs.2sev is a suppressor of interommatidial bristle phenotype of Ras85D^V12.UAS, Scer\GAL4^hs.2sev

(Kim et al., 2006)

S6kII^{K231R.UAS.Tag:MYC}, Scer\GAL4^hs.2sev is a suppressor of rhabdomere phenotype of Ras85D^V12.UAS, Scer\GAL4^hs.2sev

(Kim et al., 2006)

S6kII^{K231R.UAS.Tag:MYC}, Scer\GAL4^hs.2sev is a suppressor of ommatidium phenotype of Ras85D^V12.UAS, Scer\GAL4^hs.2sev

(Kim et al., 2006)

S6kII^{K231R.UAS.Tag:MYC}, Scer\GAL4^hs.2sev is a suppressor of rhabdomere phenotype of Raf^UAS.F179, Scer\GAL4^hs.2sev

(Kim et al., 2006)

S6kII^{K231R.UAS.Tag:MYC}, Scer\GAL4^hs.2sev is a suppressor of ommatidium phenotype of Raf^UAS.F179, Scer\GAL4^hs.2sev

(Kim et al., 2006)

S6kII^{K231R.UAS.Tag:MYC}, Scer\GAL4^hs.2sev is a suppressor of interommatidial bristle phenotype of Raf^UAS.F179, Scer\GAL4^hs.2sev

(Kim et al., 2006)

S6kII^{K231R.UAS.Tag:MYC}/Scer\GAL4^hs.2sev is a suppressor of eye photoreceptor cell | increased number phenotype of sev^S11.Tag:MYC

(Kim et al., 2006)

Additional Comments

Genetic Interactions

Statement

Reference

Expression of S6kII^{K231R.Scer\UAS.T:Hsap\MYC}, under the control of Scer\GAL4^hs.2sev, causes a more severe loss of photoreceptor cells when expressed in a Ras85D^e1B/+ or rl^10a/+ background.

The roughened eye and increased rhabdomere phenotypes seen when either phl^{Scer\UAS.F179} or Ras85D^V12.Scer\UAS is expressed under the control of Scer\GAL4^hs.2sev are suppressed when S6kII^{K231R.Scer\UAS.T:Hsap\MYC} is coexpressed. Scer\GAL4^hs.2sev>S6kII^{K231R.Scer\UAS.T:Hsap\MYC} expression also suppresses the increased photoreceptor cell phenotype seen in sev^{S11.T:Hsap\MYC} flies.

(Kim et al., 2006)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescues

S6kII^{K231R.UAS.Tag:MYC}/Scer\GAL4^tim.UAS rescues S6kII^ign-Δ58-1

(Tangredi et al., 2012)

S6kII^{K231R.UAS.Tag:MYC}/Scer\GAL4^da.G32 rescues S6kII^D1

(Kim et al., 2006)

Comments

Expression of S6kII^{K231R.Scer\UAS.T:Hsap\MYC} under the control of Scer\GAL4^tim.Scer\UAS rescues the short-period locomotor activity phenotype of S6kII^ign-Δ58-1 flies.

(Tangredi et al., 2012)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

RSK^KR

(Kim et al., 2006)

S6KII^KR

(Tangredi et al., 2012)

S6kII^{K231R.Scer\UAS.T:Hsap\MYC}

S6kII^{K231R.UAS.Tag:MYC}

Name Synonyms

Secondary FlyBase IDs

References (2)

Report Sections

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