FlyBase Allele Report: Dmel\S6kII[ign-Δ58-1]

General Information

Symbol

Dmel\S6kII^ign-Δ58-1

Species

D. melanogaster

Name

FlyBase ID

FBal0215320

Feature type

allele

Associated gene

Dmel\S6kII

Associated Insertion(s)

P{?lacW}S6kII^ign-Δ58-1

Carried in Construct

Also Known As

Df(1)ign^Δ58/1

Key Links

Allele class

loss of function allele

Mutagen

P-element activity

Nature of the Allele

Allele class

loss of function allele

(Putz et al., 2004)

Mutagen

P-element activity

(Putz et al., 2004)

Progenitor genotype

P{lacW}S6kII^ign-P1

(Putz et al., 2004)

Associated Insertion(s)

P{?lacW}S6kII^ign-Δ58-1

Cytology

Description

Imprecise excision of the P{lacW} element, resulting in deletion of 4.8kb of the S6kII gene, removing all of the S6kII coding region. Part of the P{lacW} element is retained.

(Putz et al., 2004)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Disease

Evidence

References

model of Coffin-Lowry syndrome

CEA

(Beck et al., 2015)

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal learning (with S6kII^ign-Δ24-3)

(Putz et al., 2004)

abnormal locomotor rhythm

(Tangredi et al., 2012, Akten et al., 2009)

abnormal memory

(Putz et al., 2004)

abnormal neuroanatomy

(Beck et al., 2015)

abnormal neuroanatomy | third instar larval stage

(Fischer et al., 2009)

abnormal neurophysiology

(Beck et al., 2015)

Phenotype Manifest In

embryonic/larval neuromuscular junction

(Beck et al., 2015)

mitochondrion

(Beck et al., 2015)

NMJ bouton

(Beck et al., 2015)

NMJ bouton | increased number

(Beck et al., 2015)

NMJ bouton | third instar larval stage

(Fischer et al., 2009)

presynaptic active zone

(Beck et al., 2015)

Detailed Description

Statement

Reference

NMJ size is significantly reduced in S6kII^ign-Δ58-1 mutants compared with wild type controls. The number and area of pre-synaptic active zones per NMJ is significantly reduced in S6kII^ign-Δ58-1 mutants. The number and area of post-synaptic GluRIID fields are also reduced. The ratio between active zones and GluRIID fields is unchanged from wild type and no apposition defects are observed. Bouton number is increased, but they are smaller in size and contain fewer synaptic sites.

The amplitude of spontaneous miniature excitatory junctional potentials is reduced in S6kII^ign-Δ58-1 mutant larvae.

In the axons of S6kII^ign-Δ58-1 mutant larvae, fewer mitochondria are transported in the anterograde direction compared with controls. The percentage of stationary mitochondria is significantly increased. Transport of mitochondria in the retrograde direction is unaffected.

(Beck et al., 2015)

Mutant flies have a short locomotor activity circadian period compared to wild type.

(Tangredi et al., 2012)

The circadian periodicity of locomotor activity in S6kII^ign-Δ58-1 mutant adults at 20'C is > 1hour shorter than that seen in controls. At higher temperatures, this difference with controls is reduced. Resetting of the circadian cycle by light occurs normally in these animals.

(Akten et al., 2009)

Absolute bouton number at the neuromuscular junction (NMJ) at segment A3 in muscle 6/7 is significantly higher in S6kII^ign-Δ58-1 third instar larvae compared to controls. The bouton number per muscle area is also significantly higher in the mutant larvae compared to controls. Both type 1s and type 1b boutons show an increase in absolute bouton number at the NMJ and an increase in bouton number per muscle area.

The average number of synapses per bouton and the size of synapses at the NMJ of S6kII^ign-Δ58-1 larvae is not significantly different from that of wild-type larvae.

(Fischer et al., 2009)

S6kII^ign-Δ24-3/S6kII^ign-Δ58-1 flies show reduced operant conditioning measured by heat box learning in both genders. However, S6kII^ign-Δ58-1 homozygotes do not show this phenotype.

S6kII^ign-Δ58-1 males and females show an impairment in pavlovian olfactory discrimination learning, with a 40% lower performance index in 3 min, 30 min and 3 hour memory. However, S6kII^ign-Δ58-1/Y; S6kII^t6.5/+ flies perform as well as wild-type flies in pavlovian oflactory conditioning memory tests.

(Putz et al., 2004)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

S6kII^ign-Δ58-1 has abnormal locomotor rhythm phenotype, suppressible by CkIIβ^And

(Akten et al., 2009)

S6kII^ign-Δ58-1 has abnormal locomotor rhythm phenotype, suppressible by CkIIα^Tik

(Akten et al., 2009)

S6kII^ign-Δ58-1 has abnormal neuroanatomy | larval stage phenotype, suppressible | partially by rl[+]/rl^10a

(Fischer et al., 2009)

NOT Suppressor of

Statement

Reference

S6kII^ign-Δ58-1 is a non-suppressor of abnormal locomotor rhythm phenotype of CkIIβ^And

(Akten et al., 2009)

S6kII^ign-Δ58-1 is a non-suppressor of abnormal locomotor rhythm phenotype of CkIIα^Tik

(Akten et al., 2009)

Phenotype Manifest In

Suppressed by

Statement

Reference

S6kII^ign-Δ58-1 has NMJ bouton | larval stage phenotype, suppressible | partially by rl[+]/rl^10a

(Fischer et al., 2009)

Additional Comments

Genetic Interactions

Statement

Reference

CkIIβ^And/Y or CkIIα^Tik/Y suppress the shorter circadian periodicity of locomotor activity seen in S6kII^ign-Δ58-1/Y mutant adults. The resulting animals have a longer circadian periodicity of locomotor activity than wild-type or S6kII^ign-P1/Y controls - a phenotype identical to that seen in S6kII^ign-Δ58-1/Y mutant adults.

(Akten et al., 2009)

The increase in bouton number per muscle area that is seen at the neuromuscular junction (NMJ) in S6kII^ign-Δ58-1 larvae is significantly suppressed by rl^10a/+, although the increase in absolute bouton number that is seen at the NMJ in S6kII^ign-Δ58-1 larvae is not suppressed by rl^10a/+.

S6kII^ign-Δ58-1/Y ; rl^Sem/+ double mutant larvae do not have a higher number of boutons at the neuromuscular junction than either single mutant.

(Fischer et al., 2009)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

S6kII^ign-Δ58-1 is rescued by S6kII^UAS.cBa/Scer\GAL4^tim.UAS

(Tangredi et al., 2012)

S6kII^ign-Δ58-1 is rescued by S6kII^UAS.cBa/Scer\GAL4^P2.4.Pdf

(Tangredi et al., 2012)

S6kII^ign-Δ58-1 is rescued by S6kII^{K231R.UAS.Tag:MYC}/Scer\GAL4^tim.UAS

(Tangredi et al., 2012)

S6kII^ign-Δ58-1 is rescued by Scer\GAL4^P2.4.Pdf/S6kII^{T732E.UAS.Tag:MYC}

(Tangredi et al., 2012)

S6kII^ign-Δ58-1 is rescued by S6kII^t6.5

(Akten et al., 2009)

S6kII^ign-Δ58-1 is rescued by S6kII^EGFP

(Fischer et al., 2009)

S6kII^ign-Δ58-1 is rescued by S6kII^t6.5

(Fischer et al., 2009)

Partially rescued by

S6kII^ign-Δ58-1 is partially rescued by S6kII^{+tCH322-75N12}

(Beck et al., 2015)

S6kII^ign-Δ58-1 is partially rescued by Scer\GAL4^tim.PE/S6kII^UAS.cRa

(Akten et al., 2009)

S6kII^ign-Δ58-1 is partially rescued by S6kII^UAS.cRa/Scer\GAL4^tim.UAS

(Akten et al., 2009)

S6kII^ign-Δ58-1 is partially rescued by S6kII^UAS.cRa/Scer\GAL4^P2.4.Pdf

(Akten et al., 2009)

S6kII^ign-Δ58-1 is partially rescued by S6kII^UAS.cRa/Scer\GAL4^Toll-6-D42

(Fischer et al., 2009)

Not rescued by

S6kII^ign-Δ58-1 is not rescued by S6kII^{R902A.UAS.Tag:MYC}/Scer\GAL4^tim.UAS

(Tangredi et al., 2012)

S6kII^ign-Δ58-1 is not rescued by S6kII^{K213R.R902A.UAS.Tag:MYC}/Scer\GAL4^tim.UAS

(Tangredi et al., 2012)

S6kII^ign-Δ58-1 is not rescued by S6kII^{K597M.UAS.Tag:MYC}/Scer\GAL4^P2.4.Pdf

(Tangredi et al., 2012)

S6kII^ign-Δ58-1 is not rescued by S6kII^{T732A.UAS.Tag:MYC}/Scer\GAL4^P2.4.Pdf

(Tangredi et al., 2012)

S6kII^ign-Δ58-1 is not rescued by S6kII^{S515A.UAS.Tag:MYC}/Scer\GAL4^tim.UAS

(Tangredi et al., 2012)

S6kII^ign-Δ58-1 is not rescued by S6kII^{S515D.UAS.Tag:MYC}/Scer\GAL4^tim.UAS

(Tangredi et al., 2012)

Comments

Expression of S6kII^{+tCH322-75N12} suppresses the reduction in NMJ size, and reduced number and area of pre-synaptic active zones seen in S6kII^ign-Δ58-1 mutants, but does not suppress the reduced number and area of post-synaptic GluRIID fields. The reduction in spontaneous miniature excitatory junctional potential (mini) amplitude is not rescued. Frequency of minis and quantal content (the number of vesicles releases per action potential) are similar to wild type.

(Beck et al., 2015)

Expression of S6kII^Scer\UAS.cBa under the control of Scer\GAL4^tim.Scer\UAS or Scer\GAL4^P2.4.Pdf completely rescues the short-period locomotor activity phenotype of S6kII^ign-Δ58-1 flies.

Expression of S6kII^{K231R.Scer\UAS.T:Hsap\MYC} under the control of Scer\GAL4^tim.Scer\UAS rescues the short-period locomotor activity phenotype of S6kII^ign-Δ58-1 flies.

Expression of S6kII^{T732E.Scer\UAS.T:Hsap\MYC} under the control of Scer\GAL4^P2.4.Pdf rescues the short-period locomotor activity phenotype of S6kII^ign-Δ58-1 flies.

Expression of S6kII^{K597M.Scer\UAS.T:Hsap\MYC} or S6kII^{T732A.Scer\UAS.T:Hsap\MYC} under the control of Scer\GAL4^P2.4.Pdf fails to rescue the short-period locomotor activity phenotype of S6kII^ign-Δ58-1 flies.

Expression of either S6kII^{R902A.Scer\UAS.T:Hsap\MYC}, S6kII^{K213R.R902A.Scer\UAS.T:Hsap\MYC}, S6kII^{S515A.Scer\UAS.T:Hsap\MYC} or S6kII^{S515D.Scer\UAS.T:Hsap\MYC} under the control of Scer\GAL4^tim.Scer\UAS fails to rescue the short-period locomotor activity phenotype of S6kII^ign-Δ58-1 flies.

(Tangredi et al., 2012)

The defects in locomotor circadian rhythm seen in S6kII^ign-Δ58-1 mutant adults are rescued by S6kII^t6.5 (insertion P{S6kII^t6.5}5-5) and partially rescued by S6k^Scer\UAS.cMa with Scer\GAL4^tim.PE, Scer\GAL4^tim.Scer\UAS or Scer\GAL4^P2.4.Pdf.

(Akten et al., 2009)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (8)

Reported As

Symbol Synonym

Df(1)ign^Δ58-1

(Putz et al., 2004)

Df(1)ign^Δ58/1

(Beck et al., 2018, Beck et al., 2015, Tangredi et al., 2012, Fischer et al., 2009)

Df(1)ignΔ58-1

(Akten et al., 2009)

RSK^Δ58/1

(Beck et al., 2015)

S6kII^ign-Δ58-1

S6kII^ign58-1

(Akten et al., 2009)

S6kII^ign

(Tangredi et al., 2012)

ign^58/1

(Kuntz et al., 2017)

Name Synonyms

Secondary FlyBase IDs

References (7)

Report Sections

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