The first two codons of S6kII has been changed from ATG CCG to ATG CAT, generating a NsiI restriction site. The EGFP tag has been inserted at the N-terminal end of S6kII using the NsiI restriction site.
S6kIIEGFP rescues S6kIIign-Δ58-1
The increased absolute bouton number which is seen at the neuromuscular junction in S6kIIign-Δ58-1 larvae is rescued by S6kIIT:Avic\GFP-EGFP.