FlyBase Allele Report: Dmel\fru[sat15]

General Information

Symbol

Dmel\fru^sat15

Species

D. melanogaster

Name

FlyBase ID

FBal0031285

Feature type

allele

Associated gene

Dmel\fru

Associated Insertion(s)

Carried in Construct

Key Links

Allele class

Mutagen

Delta2-3 transposase

Nature of the Allele

Allele class

Mutagen

Delta2-3 transposase

(Ito et al., 1996)

Progenitor genotype

P{lwB}fru^sat

(Anand et al., 2001)

fru^sat

(Ito et al., 1996, Ito et al., 1995)

Caused by aberration

Df(3R)fru^sat15

Cytology

Description

Deletion beginning 20bp into the inverted repeat of the P{lwB}fru^sat element and removing all nucleotides from 96,551 to 13,839 of AE003722 (including the entire fru common coding region).

(Anand et al., 2001)

Imprecise excision of P{lwB} element, together with a deletion of at least 17kb proximal to the insertion point.

(Ito et al., 1996)

Imprecise excision of the P-element of fru^sat.

(Ito et al., 1995)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal courtship behavior (with fru^4-40)

(Chen et al., 2021)

abnormal courtship behavior (with fru^ΔC)

(Billeter et al., 2006)

abnormal courtship behavior | male (with fru^4-40)

(Fan et al., 2013, Anand et al., 2001)

abnormal courtship behavior | male (with fru^M5)

(Anand et al., 2001)

abnormal mating (with fru¹)

(Lee et al., 2001)

abnormal neuroanatomy (with Df(3R)fru^w24)

(Song et al., 2002)

abnormal neuroanatomy (with fru^AJ96u3)

(Song et al., 2002)

abnormal neuroanatomy (with fru^w12)

(Song et al., 2002)

abnormal neuroanatomy (with fru^w12), with Scer\GAL4^sca-537.4, fru^MA.UAS.cSa

(Song et al., 2002)

abnormal neuroanatomy (with fru^w12), with Scer\GAL4^sca-537.4, fru^MB.UAS.cSa

(Song et al., 2002)

abnormal neuroanatomy (with fru^w12), with Scer\GAL4^sca-537.4, fru^MC.UAS.cSa

(Song et al., 2002)

abnormal neuroanatomy | adult stage | female (with fru^P1.LexA), with Scer\GAL4^Poxn.14, fru^{MC.UAS.Tag:AU1}

(Mellert et al., 2010)

abnormal neuroanatomy | adult stage | male (with fru^P1.LexA)

(Mellert et al., 2010)

abnormal song (with fru^ΔC)

(Neville et al., 2014)

decreased rate of movement | male (with fru^4-40)

(Anand et al., 2001)

decreased rate of movement | male (with fru^M5)

(Anand et al., 2001)

lethal (with fru^w9)

(Anand et al., 2001)

lethal (with fru^w27)

(Anand et al., 2001)

lethal | recessive

(Ito et al., 1996, Ito et al., 1995)

lethal - all die before end of prepupal stage (with fru^AJ96u3)

(Song et al., 2002)

lethal - all die before end of pupal stage (with fru^w12)

(Anand et al., 2001)

male semi-sterile (with fru^0-1)

(Anand et al., 2001)

male semi-sterile (with fru¹)

(Anand et al., 2001)

male sterile (with fru³)

(Anand et al., 2001)

male sterile (with fru^4-40)

(Anand et al., 2001)

male sterile (with fru⁴)

(Anand et al., 2001)

male sterile (with fru^M5)

(Anand et al., 2001)

partially lethal - majority live (with fru^ΔC)

(Billeter et al., 2006)

semi-fertile (with fru^ΔC)

(Billeter et al., 2006)

some die during prepupal stage | recessive (with fru^AJ96u3)

(Song et al., 2002)

some die during pupal stage (with fru^w12)

(Anand et al., 2001)

viable (with fru^4-40)

(Anand et al., 2001)

viable (with fru^M5)

(Anand et al., 2001)

Phenotype Manifest In

dMP2 neuron (with fru^w12)

(Song et al., 2002)

embryonic/larval glial cell (with fru^w12)

(Song et al., 2002)

larval longitudinal connective (with Df(3R)fru^w24)

(Song et al., 2002)

larval longitudinal connective (with fru^AJ96u3)

(Song et al., 2002)

larval longitudinal connective (with fru^w12)

(Song et al., 2002)

larval longitudinal connective (with fru^w12), with Scer\GAL4^sca-537.4, fru^MA.UAS.cSa

(Song et al., 2002)

larval longitudinal connective (with fru^w12), with Scer\GAL4^sca-537.4, fru^MB.UAS.cSa

(Song et al., 2002)

larval longitudinal connective (with fru^w12), with Scer\GAL4^sca-537.4, fru^MC.UAS.cSa

(Song et al., 2002)

leg joint (with fru^w12)

(Anand et al., 2001)

macrochaeta | ectopic (with fru^w12)

(Anand et al., 2001)

muscle cell of male abdominal 5 muscle (with fru^ΔC)

(Neville et al., 2014)

presumptive embryonic/larval central nervous system (with Df(3R)fru^w24)

(Song et al., 2002)

presumptive embryonic/larval central nervous system (with fru^AJ96u3)

(Song et al., 2002)

presumptive embryonic/larval central nervous system (with fru^w12)

(Song et al., 2002)

presumptive embryonic/larval central nervous system (with fru^w12), with Scer\GAL4^sca-537.4, fru^MA.UAS.cSa

(Song et al., 2002)

presumptive embryonic/larval central nervous system (with fru^w12), with Scer\GAL4^sca-537.4, fru^MB.UAS.cSa

(Song et al., 2002)

presumptive embryonic/larval central nervous system (with fru^w12), with Scer\GAL4^sca-537.4, fru^MC.UAS.cSa

(Song et al., 2002)

prothoracic leg taste bristle chemosensory neuron | female (with fru^P1.LexA), with Scer\GAL4^Poxn.14, fru^{MC.UAS.Tag:AU1}

(Mellert et al., 2010)

prothoracic leg taste bristle chemosensory neuron | male (with fru^P1.LexA)

(Mellert et al., 2010)

vMP2 neuron (with fru^w12)

(Song et al., 2002)

wing (with fru^w12)

(Anand et al., 2001)

Detailed Description

Statement

Reference

fru^sat15/Df(3R)fru^4-40 males lack the muscle of Lawrence.

The interpulse interval is significantly longer than normal and sine song is completely absent in fru^ΔC/fru^sat15 males.

(Neville et al., 2014)

Midline crossing by foreleg gustatory receptor neuron (GRN) axons is nearly absent in fru^P1.LexA/fru^sat15 XY animals.

(Mellert et al., 2010)

Flies transheterozygous for fru^ΔC and fru^sat15 exhibit approximately 13% fertility compared to wild-type. They also experience reduced viability, with approximately 32% the wild-type level of male and female progeny surviving.

(Billeter et al., 2006)

15.0% of fru^w12/fru^sat15 embryos have abnormal Fas2-positive fascicles and 16.3% have defects in the distribution and pattern of BP102-positive axons in the central nervous system. Axonal outgrowth of the dMP2 and vMP2 neurons can be delayed in these embryos, while in some cases the growth cone appears to initiate growth in an abnormal direction. 15.8% of fru^sat15/fru^AJ96u3 embryos have abnormal Fas2-positive fascicles and 17.4% have defects in the distribution and pattern of BP102-positive axons in the central nervous system. 12.4% of fru^sat15/Df(3R)fru^w24 embryos have abnormal Fas2-positive fascicles and 18.4% have defects in the distribution and pattern of BP102-positive axons in the central nervous system. fru^sat15/fru^M5 and fru^sat15/fru^4-40 embryos have normal Fas2-positive and BP102-positive axon tracts in the central nervous system. The average number of repo-positive glial cells is not different from wild type in fru^w12/fru^sat15 embryos, although the pattern of the repo-positive glial cells is slightly abnormal. The Fas2-positive and BP102-positive axon tracts in the central nervous system are defective in virtually all fru^w12/fru^sat15 embryos which are expressing fru^{MA.Scer\UAS.cSa}, fru^{MB.Scer\UAS.cSa} or fru^{MC.Scer\UAS.cSa} under the control of Scer\GAL4^sca-537.4.

(Song et al., 2002)

2% fru^w12/fru^sat15 adult escapers are seen. 5% fru^sat15/fru^w27 adult escapers are seen. 32% of fru¹/fru^sat15 males are fertile (this may reflect genetic background effects). 33% of fru^0-1/fru^sat15 males are fertile (this may reflect genetic background effects). Male-female courtship as measured by courtship index (CI) or wing extension index (WEI) is completely abolished in fru^sat15/fru^4-40, fru^sat15/fru^M5 males. fru^sat15/fru^M5 or fru^sat15/fru^4-40 males show substantial chaining. fru^w12/fru^sat15 adults that have been dissected from the pupal case show minor external defects, such as uninflated wings, wings held at a 70-90^o angle to the body, leg joint defects and duplicated bristles. The number and pattern of divisions of larval neuroblasts in the thoracic ganglia or brain appears normal in fru^sat15/Df(3R)fru^w24 animals. The male-specific abdominal neuroblasts are present and actively dividing in fru^sat15/Df(3R)fru^w24 males.

(Anand et al., 2001)

fru¹/fru^sat15 males mated individually to a single virgin female show vigorous courtship behaviour, comparable to that of wild-type males. High levels of abdominal bending are seen in the males that show courtship behaviour. However, the proportion of males that mate is reduced compared to wild type; 18% of the transheterozygous males court but do not mate. The fertility of the males (as assayed by the ability of the mated females to produce progeny) is reduced compared to wild-type males. The transheterozygous males show longer than normal mating-initiation latencies compared to heterozygous controls. Mating duration is also longer than normal and shows a far more scattered distribution than that of wild-type males.

The number of progeny (number of resulting pupae) obtained from a fertile mating between a fru¹/fru^sat15 male and a wild-type female are not significantly different to wild type.

(Lee et al., 2001)

fru¹/fru^sat15 males show homosexual courtship activity.

(Ito et al., 1996)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT Enhancer of

Statement

Reference

fru^sat15/fru^4-40 is a non-enhancer of abnormal courtship behavior | female phenotype of retn^z2-428/retn⁰⁵⁰⁹⁶

(Ditch et al., 2005)

NOT Suppressor of

Statement

Reference

fru^sat15/fru^4-40 is a non-suppressor of abnormal courtship behavior | female phenotype of retn^z2-428/retn⁰⁵⁰⁹⁶

(Ditch et al., 2005)

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

fru^P1.LexA/fru^sat15 is rescued by fru^{MC.UAS.Tag:AU1}/Scer\GAL4^Poxn.14

(Mellert et al., 2010)

Partially rescued by

fru^w12/fru^sat15 is partially rescued by fru^C.UAS.cSa/Scer\GAL4^sca-537.4

(Song et al., 2002)

fru^w12/fru^sat15 is partially rescued by Scer\GAL4^sca-537.4/fru^A.UAS.cSa

(Song et al., 2002)

Not rescued by

fru^w12/fru^sat15 is not rescued by Scer\GAL4^elav-C155/fru^B.UAS.cSa

(Song et al., 2002)

fru^w12/fru^sat15 is not rescued by Scer\GAL4^sca-537.4/fru^B.UAS.cSa

(Song et al., 2002)

fru^w12/fru^sat15 is not rescued by Scer\GAL4^elav-C155/fru^C.UAS.cSa

(Song et al., 2002)

fru^w12/fru^sat15 is not rescued by Scer\GAL4^elav-C155/fru^A.UAS.cSa

(Song et al., 2002)

Comments

Male-typical crossing of the midline by foreleg gustatory receptor neuron (GRN) axons is restored in XY fru^P1.LexA/fru^sat15 animals by expression of fru^{MC.Scer\UAS.T:BPV-1\AU1} under the control of Scer\GAL4^Poxn.14.

(Mellert et al., 2010)

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

66693

Df(3R)fru^sat15, fru^sat15/TM6B, Tb¹

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

fru^Mnull

(Neville et al., 2014)

fru^Sat15

(Chen et al., 2021, Garner et al., 2018)

fru^sat15

(Rajan et al., 2023, Chowdhury et al., 2020, Fan et al., 2013, Latham et al., 2013, Mellert et al., 2010)

sat¹⁵

(Lee et al., 2001, Ito et al., 1995)

Name Synonyms

Secondary FlyBase IDs

References (17)

Report Sections

Open Close