Approximately 14% of wild-type females expressing fruMA.Scer\UAS.cSa under the control of Scer\GAL4fru.16 develop one to three ectopic 'male' serotonergic neurons, sending projections into the abdominal nerve trunk. Ectopic serotonergic terminals innervated the female internal reproductive organs, mostly the calyx of the ovaries but also, more rarely, the uterus and the base of the spermathecae. These serotonergic terminals are never present in virgin or fertilised wild-type females. The ectopic serotonergic neurons and their associated terminalia are analogous to those innervating the male reproductive organs.
The Fas2-positive and BP102-positive axon tracts in the central nervous system are defective in virtually all fruw12/frusat15 embryos which are expressing fruMA.Scer\UAS.cSa under the control of Scer\GAL4sca-537.4.
Scer\GAL4fru.16/fruMA.UAS.cSa fails to rescue fru3/fruΔC
Scer\GAL4fru.16/fruMA.UAS.cSa fails to rescue fru3
Expression of fruMA.Scer\UAS.cSa, under the control of Scer\GAL4fru.16 in a fru3/fru3 background does not restore male fertility.
Expression of fruMA.Scer\UAS.cSa under the control of Scer\GAL4fru.16 fails to rescue fertility levels in fru3/fruΔC mutants.
Expression of fruMA.Scer\UAS.cSa under the control of Scer\GAL4fru.16, in fru3 homozygotes and fru3/fruΔC transheterozygotes fails to rescue the formation of the muscle of Lawrence in the male and fails to induce formation of muscle of Lawrence-like features and neuromuscular junctions in the female.
Expression of fruMA.Scer\UAS.cSa under the control of Scer\GAL4fru.16 does not significantly rescue differentiation of the male serotonergic neurons in fruΔC/fru3 mutants.