FlyBase Allele Report: Scer\GAL4[fru.16]

General Information

Symbol

Scer\GAL4^fru.16

Species

S. cerevisiae

Name

FlyBase ID

FBal0246734

Feature type

allele

Associated gene

Scer\GAL4

Associated Insertion(s)

Carried in Construct

P{fru-GAL4.16}

Also Known As

fru(16)-gal4

Transgenic product class

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

Mutagen

in vitro construct

(Billeter and Goodwin, 2004)

Progenitor genotype

Carried in construct

P{fru-GAL4.16}

Cytology

Description

16kb of sequence from upstream of the fru P1 RNA start site is fused upstream sequence encoding a GAL4 driver. The fru sequences consist of nucleotides 176304 to 161565 of GenBank accession AE003722.

(Billeter and Goodwin, 2004)

Allele components

Component

Use(s)

Encoded product / tool

GAL4

binary expression system - driver

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

distribution deduced from reporter (Gal4 UAS)

Stage

Tissue/Position (including subcellular localization)

Reference

late third instar larval stage -- adult stage & male

central nervous system | restricted

(Billeter and Goodwin, 2004)

adult stage

second segment of antenna | restricted

(Billeter and Goodwin, 2004)

Johnston organ | restricted

(Billeter and Goodwin, 2004)

leg | restricted

(Billeter and Goodwin, 2004)

trochanter | restricted

(Billeter and Goodwin, 2004)

femur | restricted

(Billeter and Goodwin, 2004)

tibia | restricted

(Billeter and Goodwin, 2004)

metatarsus | restricted

(Billeter and Goodwin, 2004)

tarsal segment 2 | restricted

(Billeter and Goodwin, 2004)

tarsal segment 3 | restricted

(Billeter and Goodwin, 2004)

tarsal segment 4 | restricted

(Billeter and Goodwin, 2004)

pretarsus | restricted

(Billeter and Goodwin, 2004)

tegula | restricted

(Billeter and Goodwin, 2004)

wing | restricted

(Billeter and Goodwin, 2004)

anterior wing margin | restricted

(Billeter and Goodwin, 2004)

haltere pedicel | restricted

(Billeter and Goodwin, 2004)

scabellum | restricted

(Billeter and Goodwin, 2004)

larval ventral nerve cord | restricted

(Billeter and Goodwin, 2004)

neuron of muscle cell of male abdominal 5 muscle

(Billeter and Goodwin, 2004)

neuron of seminal vesicle

(Billeter and Goodwin, 2004)

neuron of testicular duct

(Billeter and Goodwin, 2004)

neuron of anterior ejaculatory duct

(Billeter and Goodwin, 2004)

neuron of ejaculatory bulb

(Billeter and Goodwin, 2004)

neuron of phallus

(Billeter and Goodwin, 2004)

Additional Information

Statement

Reference

ScerGAL4^fru.16 generates maximal expression in 5-day old adults. Expression is observed in the mediolateral part of the second antennal segment. Expression is observed in all three pairs of legs: in approximately three cells of the tibia, approximately six cells in each of the first to the fourth tarsi, and approximately ten cells in the most distal tarsal segment. Expression is seen within approximately 20 cells of the tegula, and in a large cluster of cells in the dorsal-proximal part of the radius with a less compact cluster of cells more distally between the first and second septum of the radius. Expression of ScerGAL4^fru.16 is seen in neurons innervating the muscle of Lawrence (male abdominal 5 muscle), the seminal vesicles, the testicular duct, the anterior ejaculatory duct, the ejaculatory bulbs and the aedeagus (insect penis).

(Billeter and Goodwin, 2004)

Marker for

Reflects expression of

fru

(Billeter and Goodwin, 2004)

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References