FlyBase Allele Report: Dmel\Uba1[s3484]

General Information

Symbol

Dmel\Uba1^s3484

Species

D. melanogaster

Name

FlyBase ID

FBal0009310

Feature type

allele

Associated gene

Dmel\Uba1

Associated Insertion(s)

P{lacW}Uba1^s3484

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

P-element activity

Nature of the Allele

Allele class

Mutagen

P-element activity

Progenitor genotype

Associated Insertion(s)

P{lacW}Uba1^s3484

Cytology

Description

The P{lacW} insertion is at 436bp after the start of the open reading frame, within intron 1.

(Watts et al., 2003)

Allele components

Component

Use(s)

Inserted element

P{lacW}

enhancer trap

Encoded product / tool

lacZ

reporter enzyme

Tagged with

Tag:NLS(P)

nuclear protein localization tag

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

transposable_element_insertion_site

2R:9,692,053..9,692,053

(Schuldiner et al., 2008, Kuo et al., 2005, Watts et al., 2003, Spradling et al., 1999, BDGP Project Members, 1994-1999)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neuroanatomy | pupal stage

(Kuo et al., 2005)

abnormal neuroanatomy | somatic clone

(Watts et al., 2003)

lethal

(Colin et al., 2014)

lethal (with Uba1^D6)

(Lee et al., 2008)

lethal (with Uba1^H33)

(Lee et al., 2008)

lethal (with Uba1^H42)

(Lee et al., 2008)

lethal (with Uba1^UY3010)

(Colin et al., 2014)

lethal | recessive

(Spradling et al., 1999, BDGP Project Members, 1994-1999)

viable (with Uba1^UY3010), with Scer\GAL4^da.G32

(Colin et al., 2014)

visible | somatic clone

(Lee et al., 2008)

Phenotype Manifest In

dendrite | somatic clone

(Watts et al., 2003)

dendritic arborizing neuron | P-stage

(Kuo et al., 2005)

eye | somatic clone

(Lee et al., 2008)

eye disc | somatic clone

(Lee et al., 2008)

mushroom body & axon | somatic clone

(Watts et al., 2003)

neuroblast | somatic clone

(Watts et al., 2003)

Detailed Description

Statement

Reference

Uba1 mutant clones in sensory neurons in adult wing do not display any defects in injury-induced axon degeneration (following an axotomy, the severed axons are cleared away normally).

(Neukomm et al., 2017)

Uba1^s3484/Uba1^s3484 flies exhibit complete lethality.

Uba1^UY3010/Uba1^s3484 flies are viable in combination with Scer\GAL4^da.G32, but exhibit total lethality in the absence of a GAL4 driver.

(Colin et al., 2014)

Third larval instar discs are overgrown in mosaic animals. Adults containing homozygous clones have overgrown eyes.

(Lee et al., 2008)

Uba1^s3484 mutants fail to efficiently sever their larval dendrites during metamorphosis, with the retention of approximately 45% of large dendritic branches at 20 hours after pupal formation.

(Kuo et al., 2005)

Mutant neuroblast clones show grossly normal axonal projections to both the dorsal and medial lobes. However there are fewer cells in mutant clones as compared to wild-type. Pruning defects are evident at 18h after puparium formation (APF) indicated by the persistence of continuous larval branches. Dendrite pruning is also inhibited in clones. Although mutant mushroom body neurons survive into adulthood more than 10 days after clone induction, abnormal morphology of axons is clearly visible.

(Watts et al., 2003)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhancer of

Statement

Reference

Uba1[+]/Uba1^s3484 is an enhancer of visible phenotype of Scer\GAL4^GMR.PF, bchs^EP2299

(Simonsen et al., 2007)

NOT Suppressor of

Statement

Reference

Uba1^s3484 is a non-suppressor of abnormal neuroanatomy | somatic clone | adult stage phenotype of Sarm^{ΔARM.UAS.Tag:MYC}, Scer\GAL4^VGlut-OK371

(Neukomm et al., 2017)

Uba1[+]/Uba1^s3484 is a non-suppressor of partially lethal phenotype of Mmus\Bax^UAS.cGa, Scer\GAL4^vg.PM

(Colin et al., 2014)

Uba1[+]/Uba1^s3484 is a non-suppressor of visible | adult stage phenotype of Mmus\Bax^UAS.cGa, Scer\GAL4^vg.PM

(Colin et al., 2014)

Phenotype Manifest In

Enhancer of

Statement

Reference

Uba1[+]/Uba1^s3484 is an enhancer of eye phenotype of Scer\GAL4^GMR.PF, bchs^EP2299

(Simonsen et al., 2007)

NOT Suppressor of

Statement

Reference

Uba1^s3484 is a non-suppressor of sensory neuron | somatic clone | adult stage phenotype of Sarm^{ΔARM.UAS.Tag:MYC}, Scer\GAL4^VGlut-OK371

(Neukomm et al., 2017)

Uba1^s3484 is a non-suppressor of axon | somatic clone | adult stage phenotype of Sarm^{ΔARM.UAS.Tag:MYC}, Scer\GAL4^VGlut-OK371

(Neukomm et al., 2017)

Uba1[+]/Uba1^s3484 is a non-suppressor of wing | adult stage phenotype of Mmus\Bax^UAS.cGa, Scer\GAL4^vg.PM

(Colin et al., 2014)

Additional Comments

Genetic Interactions

Statement

Reference

The axon degeneration in sensory neurons clones in the adult wing expressing Ect4^{ΔARM.Scer\UAS.T:Hsap\MYC} under the control of Scer\GAL4^VGlut-OK371 can be suppressed by combination with Uba1.

(Neukomm et al., 2017)

Xenogenetic Interactions

Statement

Reference

Uba1^s3484/+ fails to suppress the notched wing phenotype and lethality observed in flies expressing Mmus\Bax^Scer\UAS.cGa under the control of Scer\GAL4^vg.PM.

(Colin et al., 2014)

Complementation and Rescue Data

Complements

Uba1⁰⁵⁶⁴²

(BDGP Project Members, 1994-1999)

Fails to complement

Comments

Images (0)

Mutant

Wild-type

Stocks (2)

Bloomington

23945

y¹ w^*; P{neoFRT}42D P{lacW}Uba1^s3484/CyO

Kyoto

114337

y^d2 w¹¹¹⁸ P{ey-FLP.N}2 P{5xglBS-lacZ.38-1}TPN1; P{neoFRT}42D P{lacW}Uba1^s3484 /CyO y⁺

Notes on Origin

Discoverer

M. Scott.

Comments

Complements: Mmp2⁰²³⁵³. Complements: ms(2)35Ci⁰²³¹⁶.

(BDGP Project Members, 1994-1999)

Precise excision of the insertion reverts the lethal and overgrowth phenotypes.

(Lee et al., 2008)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (6)

Reported As

Symbol Synonym

Uba1^S3484

(Neukomm et al., 2017, Schuldiner et al., 2008)

Uba1^s3484

(Colin et al., 2014, Lee et al., 2008, Pfleger et al., 2007, Watts et al., 2003, Huet et al., 2002)

l(2)03405^s3484

(BDGP Project Members, 1994-1999)

l(2)s3484

(Spradling et al., 1999)

l(2)s3484^s3484

uba1^s3484

(Jia et al., 2015, Simonsen et al., 2007)

Name Synonyms

Secondary FlyBase IDs

References (13)

Report Sections

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