FB2024_03 , released June 25, 2024
Allele: Dmel\Uba1UY3010
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General Information
Symbol
Dmel\Uba1UY3010
Species
D. melanogaster
Name
FlyBase ID
FBal0290349
Feature type
allele
Associated gene
Dmel\Uba1
Associated Insertion(s)
P{Mae-UAS.6.11}Uba1UY3010
Carried in Construct
Key Links
Genomic Maps

Allele class

loss of function allele

Mutagen
    Nature of the Allele
    Allele class

    loss of function allele

    (Colin et al., 2014)
    Mutagen
    Progenitor genotype
    Associated Insertion(s)
    P{Mae-UAS.6.11}Uba1UY3010
    Cytology
    Description

    P{Mae-UAS.6.11} insertion 258bp upstream of the translation initiation codon and 554bp downstream of the transcription start site, in the correct orientation to drive overexpression of Uba1 in the presence of a Scer\GAL4 driver.

    (Colin et al., 2014)
    Allele components
    Component
    Use(s)
    Inserted element
    P{Mae-UAS.6.11}
    Regulatory region(s)
    UAS
    Mutations Mapped to the Genome
    Curation Data
    Variant Molecular Consequences
    Associated Sequence Data
    DDBJ / EMBL / GenBank
    DNA sequence
    Protein sequence
     
    UniProtKB/Swiss-Prot
      UniProtKB/TrEMBL
        Expression Data
        Reporter Expression
        Additional Information
        Statement
        Reference
         
        Marker for
        Reflects expression of
        Reporter construct used in assay
        Human Disease Associations
        Disease Ontology (DO) Annotations
        Models Based on Experimental Evidence ( 0 )
        Disease
        Evidence
        References
        Modifiers Based on Experimental Evidence ( 1 )
        Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
         
        Disease-implicated variant(s)
         
        Phenotypic Data
        Phenotypic Class
        Phenotype Manifest In
        Detailed Description
        Statement
        Reference

        Expression of Uba1 with Uba1UY3010 under the control of Scer\GAL4vg.PM or Scer\GAL4ptc.PU does not result in any apparent phenotype.

        Uba1UY3010/Uba1UY3010 or Uba1UY3010/Uba1s3484 flies are viable in combination with Scer\GAL4da.G32.

        Uba1UY3010/Uba1UY3010 or Uba1UY3010/Uba1s3484, in the absence of a GAL4 allele, results in complete lethality.

        (Colin et al., 2014)
        External Data
        Interactions
        Show genetic interaction network for Enhancers & Suppressors
        Phenotypic Class
        Phenotype Manifest In
        Suppressor of
        Statement
        Reference

        Uba1UY3010, Scer\GAL4vg.PM is a suppressor of wing | adult stage phenotype of Mmus\BaxUAS.cGa, Scer\GAL4vg.PM

        (Colin et al., 2014)
        NOT Suppressor of
        Statement
        Reference

        Uba1UY3010, Scer\GAL4ptc.PU is a non-suppressor of wing | adult stage phenotype of DebclUAS.Tag:HA, Scer\GAL4ptc.PU

        (Colin et al., 2014)
        Additional Comments
        Genetic Interactions
        Statement
        Reference

        Co-expression of Uba1UY3010 fails to suppress the adult wing phenotype of flies expressing DebclScer\UAS.T:Ivir\HA1 under the control of Scer\GAL4ptc.PU.

        (Colin et al., 2014)
        Xenogenetic Interactions
        Statement
        Reference

        Over-expression of Uba1 with Uba1UY3010 under the control of Scer\GAL4vg.PM attenuates the notched wing phenotype and lethality observed in flies expressing Mmus\BaxScer\UAS.cGa under the control of Scer\GAL4vg.PM.

        (Colin et al., 2014)
        Complementation and Rescue Data
        Comments
        Images (0)
        Mutant
        Wild-type
        Stocks (0)
        Notes on Origin
        Discoverer
        External Crossreferences and Linkouts ( 0 )
        Synonyms and Secondary IDs (1)
        Reported As
        Symbol Synonym
        Uba1UY3010
        (Colin et al., 2014, Latouche et al., 2007)
        Name Synonyms
        Secondary FlyBase IDs
          References (2)