FB2024_03 , released June 25, 2024
Allele: Dmel\Uba1H33
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General Information
Symbol
Dmel\Uba1H33
Species
D. melanogaster
Name
FlyBase ID
FBal0265021
Feature type
allele
Associated gene
Dmel\Uba1
Associated Insertion(s)
Carried in Construct
Key Links
Genomic Maps

Allele class
Mutagen
Nature of the Allele
Allele class
Mutagen
ethyl methanesulfonate
(Lee et al., 2008)
Progenitor genotype
Cytology
Description

Amino acid replacement: P884L.

(Lee et al., 2008)
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
point_mutation
2R:9,689,750..9,689,750 [-]
Nucleotide change:

C9689750T

Amino acid change:

P884L | Uba1-PA; P701L | Uba1-PC

Reported amino acid change:

P884L

Comment:

Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change.

(Lee et al., 2008)
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Homozygous clones cannot be recovered in the adult eye at 29[o]C (they undergo apoptosis). However, despite the absence of mutant clones, these eyes are often larger than normal and contain folds, indicating overgrowth. Third larval instar discs are overgrown in mosaic animals raised at 29[o]C. Although homozygous clones are present in the overgrown discs, they are relatively small compared with controls and so do not contribute to the overgrowth.

      At 25[o]C, homozygous clones in the eye survive well and are recovered in adults.

      Homozygous clones in the eye disc contain extra interommatidial cells compared to controls at 42 hours after puparium formation.

      (Lee et al., 2008)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Suppressor of
      Statement
      Reference

      Uba1H33/Uba1H33 is a suppressor of visible phenotype of hidGMR.PG

      (Lee et al., 2008)
      Phenotype Manifest In
      Suppressor of
      Statement
      Reference

      Uba1H33/Uba1H33 is a suppressor of eye phenotype of hidGMR.PG

      (Lee et al., 2008)
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      The small eye phenotype caused by WGMR.PG is moderately strongly suppressed by inducing homozygous Uba1H33 clones in the eye. The rescued eye tissue is homozygous for Uba1H33, indicating that the suppression is cell autonomous.

      (Lee et al., 2008)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (1)
      Reported As
      Symbol Synonym
      Uba1H33
      (Chang et al., 2013, Lee et al., 2008)
      Name Synonyms
      Secondary FlyBase IDs
        References (2)