Breakpoint maps to between coordinates +187 and +188kb.
The inversion puts the 90E region into the bithorax complex at +188 kb., in the presumed iab9 region.
In Abd-Biab9-Tab/+ animals, ectopic expression of Abd-B protein is observed in the wing disc in three regions whose location in the fate map of the disc correlates well with the three islands of abdominal-like cuticle in each half of the adult T2. There is also indirect evidence for expression of Abd-B protein in subsets of muscle attachment sites throughout the animal.
In Abd-Biab9-Tab/+ animals, ectopic expression of transcripts encoding Abd-BII protein is observed in the wing disc in three regions whose location in the fate map of the disc correlates well with the three islands of abdominal-like cuticle in each half of the adult T2. There is also indirect evidence for expression of transcripts encoding Abd-BII protein in subsets of muscle attachment sites throughout the animal.
Abd-B protein is observed at greatly reduced levels in parasegment 14 and at wild type levels in parasegments 10-13.
Abd-B transcripts are observed at greatly reduced levels in parasegment 14 and at wild type levels in parasegments 10-13.
Transformed islands in the adult thorax correspond to subsets of muscle attachment cells.
Adult homozygous males have 6 normal abdominal segments. Males and females lack genitalia. Heterozygotes with Abd-Biab9-tuh-3 exhibit genital defects to a moderate degree. No effect on penetrance of head defect phenotype when in trans with Abd-Biab9-tuh-3.
Male and female genitalia and analia are missing or abnormal in homozygotes. Parasegment 14 is partially transformed into parasegment 13, and there is a rudimentary setal belt in A9 in homozygous embryos.
The Abd-Biab9-Tab mutation creates a sexually dimorphic pattern of segmental transformation that has complete penetrance and expressivity. Specific regions of the T2 notum are transformed into abdominal-like cuticle. In heterozygotes this creates black stripes in males and stripes bordered with black in females.
Embryonic transformation of A8 towards A7 and A7 towards A6.
Heterozygous adults have a pair of laterally disposed longitudinal stripes of tissue on the second thoracic segment (T2) probably corresponding to tissue from the dorsal sixth (A6) and/or seventh (A7) abdominal segments. These ectopic stripes of tissue are entirely black in males but only partially pigmented in females. Abd-Biab9-Tab/+ flies are virtually sterile and have a thin seventh tergite in males. Fertility is partially restored in the presence of a duplication for the Bithorax complex, such as Dp(3;1)P68. When hemizygous, Abd-Biab9-Tab embryos have a reduction of the posterior spiracles and filzkorper, and a tiny ninth abdominal ventral setal belt that appears as a small row of denticles posterior to the A8 setal belt.
Abd-Biab9-Tab, Dp(3;1)P68 has fertile phenotype
Complements iab-5 and iab-6 alleles.
Lewis.
E. B. Lewis.
Embryos lack the r function, as shown by their ability to complement the m- r+ allele Abd-Biab8-D14.
m+ r- mutant.
An enhancer trap element, P{lacZ-un3}B14, is inserted at 90E, the location of one of the breaks of In(3R)iab9Tab. P{lacZ-un3}B14 is expressed in the wing disc in the same three islands as those in which Abd-B class B transcript and protein are ectopically expressed, in Abd-Biab9-Tab/+ heterozygotes.