From: 	Seymour Knowles-Barley <seymour.kb@XXXX>
Subject: 	BrainTrap expression curation
Date: 	24 August 2011  17:17:24  GMT+ 01:00 
To: 	David Osumi-Sutherland <djs93@XXXX>
Dear David,
The attached XML contains details of the BrainTrap expression annotation.  This is a frozen version of the one live on the BrainTrap site, which can be downloaded here: http://fruitfly.inf.ed.ac.uk/braintrap/feed/annotations_xml
The BrainTrap database contains information on protein expression patterns in the Drosophila melanogaster brain. Further details on the are available in the open access publication: Seymour Knowles-Barley, Mark Longair, and J Douglas Armstrong (2010) BrainTrap: a database of 3D protein expression patterns in the Drosophila brain. Database (Oxford).  2010:baq005 , doi: 10.1093/database/baq005.
Drosophila protein trap strains were generated by the St Johnston Lab and the Russell Lab at the University of Cambridge, UK.
Brain images were acquired by Seymour Knowles-Barley, in the Armstrong Lab  at the University of Edinburgh. Whole brain mounts were imaged by confocal microscopy, with a background immunohistochemical label (nc82, anti-brp) added to aid the identification of brain structures. Additional immunohistochemical labelling of the EYFP protein using an anti-GFP antibody was also used in most cases. Further protocol details can be found here: http://fruitfly.inf.ed.ac.uk/braintrap/welcome/protocol
Annotations were made by Seymour Knowles-Barley, using the BrainTrap website to manually identify and label major anatomical areas of expression according to the FlyBase anatomy ontology (fly_anatomy.obo).  If you use data or images from the BrainTrap website please cite the journal article mentioned above.
Regards,
Seymour