FlyBase Gene Report: Dmel\Gdh

General Information

Symbol

Dmel\Gdh

Species

D. melanogaster

Name

Glutamate dehydrogenase

Annotation Symbol

CG5320

Feature Type

protein_coding_gene

FlyBase ID

FBgn0001098

Gene Model Status

Current

Stock Availability

15 publicly available

Enzyme Name (EC)

glutamate dehydrogenase (1.4.1.2)

glutamate dehydrogenase (NADP(+)) (1.4.1.4)

Gene Summary

Glutamate dehydrogenase (Gdh) encodes a glutamate dehydrogenase involved glutamate metabolism. [Date last reviewed: 2019-09-19] (FlyBase Gene Snapshot)

All Summaries

Gene Snapshot
Alliance
Auto summary
Gene Group
Red Book
Interactive Fly

Also Known As

GLUD

Key Links

Genomic Location

Cytogenetic map

95C13-95D1

Sequence location

3R:23,935,958..23,943,101 [-]

Recombination map

3-81

RefSeq locus

NT_033777 REGION:23935958..23943101

Sequence

Get Decorated FASTA

Genomic Maps

JBrowse

Other Genome Views

The following external sites may use different assemblies or annotations than FlyBase.

Function

Gene Ontology (GO) Annotations (12 terms)

Molecular Function (4 terms)

Terms Based on Experimental Evidence (3 terms)

CV Term

Evidence

References

enables glutamate dehydrogenase (NAD+) activity

inferred from direct assay

(Demianchuk et al., 2024, Prezioso et al., 1985, Caggese et al., 1982, Bond and Sang, 1968)

enables glutamate dehydrogenase (NADP+) activity

inferred from direct assay

(Demianchuk et al., 2024, Bond and Sang, 1968)

enables identical protein binding

inferred from direct assay

(Caggese et al., 1982)

Terms Based on Predictions or Assertions (2 terms)

CV Term

Evidence

References

enables glutamate dehydrogenase (NAD+) activity

inferred from biological aspect of ancestor with PANTHER:PTN000176230

(GO Reference Genome Project, 2011-)

enables oxidoreductase activity, acting on the CH-NH2 group of donors, NAD or NADP as acceptor

inferred from electronic annotation with InterPro:IPR014362, InterPro:IPR033922

(InterPro Project Members, 2004-)

inferred from electronic annotation with InterPro:IPR033922

(InterPro Project Members, 2004-)

Biological Process (5 terms)

Terms Based on Experimental Evidence (2 terms)

CV Term

Evidence

References

involved_in glutamate metabolic process

inferred from direct assay

(Caggese et al., 1982, Bond and Sang, 1968)

involved_in NADH oxidation

inferred from direct assay

(Caggese et al., 1982)

Terms Based on Predictions or Assertions (3 terms)

CV Term

Evidence

References

involved_in amino acid metabolic process

inferred from electronic annotation with InterPro:IPR006095, InterPro:IPR006096, InterPro:IPR006097

(InterPro Project Members, 2004-)

involved_in glutamate catabolic process

inferred from biological aspect of ancestor with PANTHER:PTN000176230

(GO Reference Genome Project, 2011-)

NOT involved_in sperm storage

traceable author statement

(Chapman, 2001)

Cellular Component (3 terms)

Terms Based on Experimental Evidence (3 terms)

CV Term

Evidence

References

located_in cytoplasm

inferred from direct assay

(Papadopoulou and Louis, 2000)

located_in cytosol

inferred from high throughput direct assay

(Lye et al., 2014)

located_in mitochondrion

inferred from high throughput direct assay

(Tan et al., 2009, Alonso et al., 2005)

inferred from direct assay

(Bond and Sang, 1968)

Terms Based on Predictions or Assertions (1 term)

CV Term

Evidence

References

is_active_in mitochondrion

inferred from biological aspect of ancestor with PANTHER:PTN000176229

(GO Reference Genome Project, 2011-)

Gene Group (FlyBase)

CH-NH2 OXIDOREDUCTASES, NAD OR NADP AS ACCEPTOR

Protein Family (UniProt)

Belongs to the Glu/Leu/Phe/Val dehydrogenases family. (P54385)

Protein Signatures (InterPro)

Catalytic Activity (EC/Rhea)

glutamate dehydrogenase (NAD+) activity

H2O + L-glutamate + NAD(+) = 2-oxoglutarate + H(+) + NADH + NH4(+) (1.4.1.2)

RHEA 15133: glutamate dehydrogenase (NADP+) activity

H2O + L-glutamate + NADP(+) = 2-oxoglutarate + H(+) + NADPH + NH4(+) (1.4.1.4)

RHEA 11612:

Summaries

Gene Snapshot

Glutamate dehydrogenase (Gdh) encodes a glutamate dehydrogenase involved glutamate metabolism. [Date last reviewed: 2019-09-19]

Gene Group (FlyBase)

CH-NH2 OXIDOREDUCTASES, NAD OR NADP AS ACCEPTOR -: CH-NH2 oxidoreductases with NAD or NADP as acceptor, include dehydrogenases that oxidize a CH-NH2 group with the reduction of NAD or NADP.

Phenotypic Description (Red Book; Lindsley and Zimm 1992)

Gdh: Glutamate dehydrogenase

Structural gene for NAD- dependent glutamate dehydrogenase [GDH (EC1.4.1.4)], which comprises six 57,000 dalton subunits. Enzyme activity inducible by addition of glutamate to medium. Nearly all larval activity can be isolated with the mitochondria.

Gene Model and Products

Number of Transcripts

Number of Unique Polypeptides

Please see the JBrowse view of Dmel\Gdh for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Structure

Protein 3D structure (Predicted by AlphaFold) (AlphaFold entry P54385)

If you don't see a structure in the viewer, refresh your browser.

Model Confidence:

Very high (pLDDT > 90)
Confident (90 > pLDDT > 70)
Low (70 > pLDDT > 50)
Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Experimentally Determined Structures

Crossreferences

Comments on Gene Model

Gene model reviewed during 5.47

Sequence Ontology: Class of Gene

nuclear_gene

protein_coding_gene

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

Gdh-RA

FBtr0089498

NM_079746

2195

562

Gdh-RB

FBtr0089496

NM_170101

1835

404

Gdh-RD

FBtr0335205

NM_206550

1796

391

Gdh-RF

FBtr0089497

NM_206551

2156

549

Additional Transcript Data and Comments

Reported size (kB)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

UniProt

RefSeq ID

GenBank

Gdh-PA

FBpp0088988

62.5

562

8.43

FBpp0088496

44.5

404

7.10

FBpp0307192

43.1

391

7.34

FBpp0088990

61.1

549

8.58

NP_996274

AAS65200

Polypeptides with Identical Sequences

None of the polypeptides share 100% sequence identity.

Additional Polypeptide Data and Comments

Reported size (kDa)

Comments

External Data

Subunit Structure (UniProtKB)

Homohexamer.

(UniProt, P54385)

Crossreferences

InterPro - A database of protein families, domains and functional sites

Linkouts

Sequences Consistent with the Gene Model

Nucleotide / Polypeptide Records

UniProt

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Gdh using the Feature Mapper tool.

External Data

Crossreferences

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

Gdh_1

Linkouts

Expression Data

Testis-specificity index

The testis specificity index was calculated from modENCODE tissue expression data by Vedelek et al., 2018 to indicate the degree of testis enrichment compared to other tissues. Scores range from -2.52 (underrepresented) to 5.2 (very high testis bias).

-1.07

Transcript Expression

in situ

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 1 -- 3

organism

Comment: maternally deposited

(Fisher et al., 2012)

embryonic stage 4 -- 6

organism | ubiquitous

(Fisher et al., 2012, Papadopoulou and Louis, 2000)

embryonic stage 7

ventral furrow

(Papadopoulou and Louis, 2000)

embryonic stage 8 -- 11

somatic mesoderm

(Papadopoulou and Louis, 2000)

head mesoderm

(Papadopoulou and Louis, 2000)

embryonic stage 9 -- 10

anterior endoderm

(Fisher et al., 2012)

head mesoderm

(Fisher et al., 2012)

organism | ubiquitous

(Fisher et al., 2012)

posterior endoderm

(Fisher et al., 2012)

trunk mesoderm

(Fisher et al., 2012)

embryonic stage 11 -- 12

anterior midgut primordium

(Fisher et al., 2012)

fat body primordium

Comment: reported as fat body specific anlage

(Fisher et al., 2012)

fat body/gonad primordium

(Fisher et al., 2012)

organism | ubiquitous

(Fisher et al., 2012)

posterior midgut primordium

(Fisher et al., 2012)

somatic muscle primordium

Comment: reported as muscle system primordium

(Fisher et al., 2012)

visceral muscle primordium

Comment: reported as muscle system primordium

(Fisher et al., 2012)

trunk mesoderm

(Fisher et al., 2012)

embryonic stage 12

larval ventral nerve cord

(Papadopoulou and Louis, 2000)

hypopharyngeal lobe

(Papadopoulou and Louis, 2000)

embryonic stage 12 -- 16

posterior midgut primordium

(Papadopoulou and Louis, 2000)

embryonic hindgut

(Papadopoulou and Louis, 2000)

anterior midgut primordium

(Papadopoulou and Louis, 2000)

embryonic stage 14 -- 16

fat body primordium

(Papadopoulou and Louis, 2000)

embryonic stage 14 -- 17

presumptive embryonic/larval central nervous system

(Papadopoulou and Louis, 2000)

embryonic stage 15 -- 17

embryonic somatic muscle cell

(Papadopoulou and Louis, 2000)

third instar larval stage

wing disc

(Papadopoulou and Louis, 2000)

leg disc

(Papadopoulou and Louis, 2000)

eye-antennal disc

Comment: except the morphogenetic furrow

(Papadopoulou and Louis, 2000)

wing pouch

(Papadopoulou and Louis, 2000)

embryonic/larval optic lobe

(Papadopoulou and Louis, 2000)

haltere disc

(Papadopoulou and Louis, 2000)

Additional Descriptive Data

From stage 12, expression is stronger in the posterior regions of the midgut than the anterior part.

(Papadopoulou and Louis, 2000)

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

immunolocalization

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 4 -- 5

organism | ubiquitous

• cytoplasm

(Papadopoulou and Louis, 2000)

embryonic stage 6 -- 7

ventral furrow

• cytoplasm

(Papadopoulou and Louis, 2000)

embryonic stage 8 -- 9

somatic mesoderm

• cytoplasm

(Papadopoulou and Louis, 2000)

head mesoderm

• cytoplasm

(Papadopoulou and Louis, 2000)

embryonic stage 12

larval ventral nerve cord

• cytoplasm

(Papadopoulou and Louis, 2000)

hypopharyngeal lobe

• cytoplasm

(Papadopoulou and Louis, 2000)

embryonic stage 12 -- 16

anterior midgut primordium

• cytoplasm

(Papadopoulou and Louis, 2000)

posterior midgut primordium

• cytoplasm

(Papadopoulou and Louis, 2000)

embryonic hindgut

• cytoplasm

(Papadopoulou and Louis, 2000)

embryonic stage 13 -- 16

embryonic Malpighian tubule

• cytoplasm

(Papadopoulou and Louis, 2000)

embryonic stage 13 -- 17

embryonic somatic muscle cell

• cytoplasm

(Papadopoulou and Louis, 2000)

embryonic stage 14 -- 16

fat body primordium

• cytoplasm

(Papadopoulou and Louis, 2000)

embryonic stage 14 -- 17

presumptive embryonic/larval central nervous system

• cytoplasm

(Papadopoulou and Louis, 2000)

third instar larval stage

eye disc

(Tiwari et al., 2014)

embryonic/larval Malpighian tubule

(Tiwari et al., 2014)

embryonic/larval salivary gland

(Tiwari et al., 2014)

mass spectroscopy

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage

adult head

• membrane

(Aradska et al., 2015)

day 5 of adulthood & male -- day 7 of adulthood & male

spermatozoon

(Wasbrough et al., 2010)

day 7 of adulthood -- day 49 of adulthood

adult heart

(Cammarato et al., 2011)

Additional Descriptive Data

Muscles are strongly stained from stage 13 on. This is earlier than seen with in situ hybridization. High magnification shows that muscles are not stained evenly with the antibody. Stain accumulates at the ends and in the center of the muscles.

(Papadopoulou and Louis, 2000)

Marker for

Subcellular Localization

CV Term

Evidence

References

located_in cytoplasm

inferred from direct assay

(Papadopoulou and Louis, 2000)

located_in cytosol

inferred from high throughput direct assay

(Lye et al., 2014)

located_in mitochondrion

inferred from high throughput direct assay

(Tan et al., 2009, Alonso et al., 2005)

inferred from direct assay

(Bond and Sang, 1968)

Expression Deduced from Reporters

Reporter: PBac{681.P.FSVS-1}Gdh^CPTI001709

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage

adult brain

Comment: low expression throughout the adult brain

(Knowles-Barley, 2011.8.24)

High-Throughput Expression Data

Associated Tools

JBrowse - Visual display of RNA-Seq signals

View Dmel\Gdh in JBrowse

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\Gdh

View exonic expression by tissue for Dmel\Gdh

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0001098

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

42832/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0001098

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0001098

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0001098

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG5320

Flygut - An atlas of the Drosophila adult midgut

FBgn0001098

Images

FlyExpress - Embryonic expression images (BDGP data)

Show all FlyExpress processed images for Dmel\Gdh

Stages(s) 4-6
Stages(s) 9-10
Stages(s) 11-12
Stages(s) 13-16

FlyBase Wiki

Image Based Resources

Alleles, Insertions, Transgenic Constructs, and Aberrations

Classical and Insertion Alleles ( 19 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 10 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of Gdh

Transgenic constructs containing regulatory region of Gdh

Aberrations (Deficiencies and Duplications) ( 1 )

Inferred from experimentation ( 1 )

Gene disrupted in

Df(3R)Exel6196

(Ryder, 2004.4.26)

Inferred from location ( 0 )

Variants

Variant Molecular Consequences

Alleles Representing Disease-Implicated Variants

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Lethality

Allele

viable

Gdh^EY07150

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

Gdh^GD11605

viable, with Scer\GAL4^Mef2.PR

Gdh^GD11605

viable, with Scer\GAL4^pnr-MD237

Gdh^GD11605

viable, with Scer\GAL4^tin.CΔ4

Gdh^GD11605

Sterility

Allele

fertile

Gdh^EY07150

Other Phenotypes

Allele

abnormal locomotor behavior | third instar larval stage, with Scer\GAL4^GMR83E12

Gdh^GD11605

abnormal starvation stress response | adult stage

Gdh^EY07150

electrophoretic variant

short lived | nutrition conditional

Gdh^EY07150

Phenotype manifest in

Allele

Orthologs

Downloads

Download All DIOPT Orthologs

Human Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Homo sapiens (Human) (2)

Hsap\GLUD1

14 of 14

Yes

Hsap\GLUD2

13 of 14

Yes

Model Organism Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Rattus norvegicus (Norway rat) (1)

Rnor\Glud1

13 of 14

Yes

Mus musculus (laboratory mouse) (1)

Mmus\Glud1

13 of 14

Yes

Xenopus tropicalis (Western clawed frog) (2)

Xtro\glud1

11 of 13

Yes

Xtro\pdk4

1 of 13

Danio rerio (Zebrafish) (2)

Drer\glud1a

14 of 14

Yes

Drer\glud1b

13 of 14

Yes

Caenorhabditis elegans (Nematode, roundworm) (1)

Cele\gdh-1

13 of 14

Yes

Anopheles gambiae (African malaria mosquito) (2)

Agam\AgaP_AGAP004362

11 of 12

Yes

Agam\AgaP_AGAP004622

6 of 12

Arabidopsis thaliana (thale-cress) (4)

Atha\GDH2

11 of 13

Yes

Atha\GDH1

10 of 13

Atha\GDH3

10 of 13

Atha\AT1G51720

6 of 13

Yes

Saccharomyces cerevisiae (Brewer's yeast) (3)

Scer\GDH1

8 of 13

Yes

Scer\GDH3

8 of 13

Yes

Scer\GDH2

3 of 13

Yes

Schizosaccharomyces pombe (Fission yeast) (2)

Spom\gdh1

8 of 12

Yes

Spom\gdh2

2 of 12

Yes

Escherichia coli (enterobacterium) (1)

Ecol\gdhA

7 of 11

Yes

Other Organism Orthologs (via OrthoDB)

Data provided directly from OrthoDB:Gdh. Refer to their site for version information.

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v9.1)

Gene Symbol

Score

Source

Alignment

Drosophila melanogaster (Fruit fly) (1)

bb8

11 of 13

Human Disease Associations

FlyBase Human Disease Model Reports

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Allele

Disease

Evidence

References

Potential Models Based on Orthology ( 1 )

Human Ortholog

Disease

Evidence

References

GLUD1; glutamate dehydrogenase 1

model of familial hyperinsulinemic hypoglycemia 6

IEA

(FlyBase, 2019-)

Modifiers Based on Experimental Evidence ( 0 )

Allele

Disease

Interaction

References

Disease Associations of Human Orthologs (via DIOPT v9.1 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

GLUD1; glutamate dehydrogenase 1

14 of 14

138130

HYPERINSULINEMIC HYPOGLYCEMIA, FAMILIAL, 6; HHF6

familial hyperinsulinemic hypoglycemia 6

GLUD2; glutamate dehydrogenase 2

13 of 14

300144

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Interactions

Summary of Physical Interactions

esyN Network Diagram

Show neighbor-neighbor interactions:

Off

Show/hide secondary interactors
(data from AllianceMine provided by esyN)

Select Layout:

Force Directed

Circle

Concentric

Legend:

Protein

RNA

Selected Interactor(s)

Other Interaction Browsers

View in FlyBase Interactions Browser View in MIST tool (external link)

Please see the Physical Interaction reports below for full details

protein-protein

Physical Interaction

Assay

References

Gdh - ATPsynB

experimental knowledge based

(Guruharsha et al., 2011)

Gdh - ATPsynD

experimental knowledge based

(Guruharsha et al., 2011)

Gdh - ATPsynF

experimental knowledge based

(Guruharsha et al., 2011)

Gdh - Act79B

experimental knowledge based

(Guruharsha et al., 2011)

Gdh - Actn

experimental knowledge based

(Guruharsha et al., 2011)

Gdh - Adh

experimental knowledge based

(Guruharsha et al., 2011)

Gdh - Argk1

experimental knowledge based

(Guruharsha et al., 2011)

Gdh - Cs1

experimental knowledge based

(Guruharsha et al., 2011)

Gdh - Gpdh1

experimental knowledge based

(Guruharsha et al., 2011)

Gdh - Mf

experimental knowledge based

(Guruharsha et al., 2011)

Gdh - Mlc1

experimental knowledge based

(Guruharsha et al., 2011)

Gdh - Mlc2

experimental knowledge based

(Guruharsha et al., 2011)

Gdh - Mpcp1

experimental knowledge based

(Guruharsha et al., 2011)

Gdh - ND-PDSW

experimental knowledge based

(Guruharsha et al., 2011)

Gdh - Nrd1

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Yoon et al., 2017)

Gdh - Ogdh1

experimental knowledge based

(Guruharsha et al., 2011)

Gdh - Prm

experimental knowledge based

(Guruharsha et al., 2011)

Gdh - Scp1

experimental knowledge based

(Guruharsha et al., 2011)

Gdh - Tm2

experimental knowledge based

(Guruharsha et al., 2011)

Gdh - mAcon1

experimental knowledge based

(Guruharsha et al., 2011)

Gdh - up

experimental knowledge based

(Guruharsha et al., 2011)

Gdh - wupA

experimental knowledge based

(Guruharsha et al., 2011)

RNA-protein

Physical Interaction

Assay

References

Gdh - Fmr1

anti bait coimmunoprecipitation, quantitative reverse transcription pcr

(Zhang et al., 2022)

Summary of Genetic Interactions

esyN Network Diagram

Show/hide secondary interactors
(data from AllianceMine provided by esyN)

esyN Network Key:

Suppression

Enhancement

Other Interaction Browsers

View in FlyBase Interactions Browser View in MIST tool (external link)

Please look at the allele data for full details of the genetic interactions

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

shop

suppressible

Gdh

(Otto et al., 2018)

External Data

Subunit Structure (UniProtKB)

Homohexamer.

(UniProt, P54385 )

Linkouts

BioGRID - A database of protein and genetic interactions.

67768

DroID - A comprehensive database of gene and protein interactions.

FBgn0001098

MIST (protein-protein) - An integrated Molecular Interaction Database

42832

Pathways

Signaling Pathways (FlyBase)

Metabolic Pathways

FlyCyc Pathways - Pathways from a BioCyc PGDB for Dmel

KEGG Metabolic Pathways - A collection of manually drawn metabolic pathway maps representing knowledge of molecular interaction, reaction and relation networks.

Reactome Metabolic Pathways - An open-source, open access, manually curated and peer-reviewed metabolic pathway database.

Glutamate and glutamine metabolism

External Data

Linkouts

KEGG Pathways - A collection of manually drawn pathway maps representing knowledge of molecular interaction, reaction and relation networks.

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

Genomic Location and Detailed Mapping Data

Chromosome (arm)

Recombination map

3-81

Cytogenetic map

95C13-95D1

Sequence location

3R:23,935,958..23,943,101 [-]

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

95C13-95D1

Limits computationally determined from genome sequence between P{PZ}l(3)04684⁰⁴⁶⁸⁴ and P{PZ}Atg6⁰⁰⁰⁹⁶

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

95D1-95D4

(Louis, 1997.2.17, Louis, 1993.12.13)

95D1-95D4

(determined by in situ hybridisation)

(Ranz et al., 1999, Papadopoulou and Louis, 1990)

95D-95D

(determined by in situ hybridisation)

(Drosopoulou et al., 1997)

Experimentally Determined Recombination Data

Location

3-81

(FlyBase, 2016)

3-82.4

(Comeron et al., 2012)

3-81.7

Left of (cM)

Right of (cM)

Notes

Stocks and Reagents

Stocks (15)

Genomic Clones (11)

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (555)

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see JBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequenced

BDGP DGC clones

LD23954

Other clones

RE31353

Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

FBgn0001098

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

RNAi and Array Information

Linkouts

DRSC - Results frm RNAi screens

FBgn0001098

Antibody Information

Laboratory Generated Antibodies

polyclonal

(Papadopoulou and Louis, 2000)

Commercially Available Antibodies

Cell Line Information

Publicly Available Cell Lines

Other Stable Cell Lines

Other Comments

Gdh has been isolated and characterised.

(Papadopoulou and Louis, 1990)

The distribution of Gdh enzyme in the larva and adult has been studied.

(Chase and Kankel, 1987)

Gdh enzyme has been isolated from the larvae of two D.melanogaster strains and characterised biochemically.

(Prezioso et al., 1985)

Structural gene for NAD⁺-dependent glutamate dehydrogenase (GDH), which comprises six 57,000 dalton subunits. Enzyme activity inducible by addition of glutamate to medium. Nearly all larval activity can be isolated with the mitochondria.

Relationship to Other Genes

Source for database merge of

Additional comments

Nomenclature History

Source for database identify of

Source for identity of: Gdh CG5320

(Kodira, 1999.11)

Nomenclature comments

Etymology

Synonyms and Secondary IDs (16)

Reported As

Symbol Synonym

CG5320

(Hallier et al., 2015, Rosa-Ferreira et al., 2015, Cammarato et al., 2011, Ni et al., 2010.12.1, Tiefenböck et al., 2010, Baltzer et al., 2009, Keleman et al., 2009.8.5, Mohammad et al., 2009, Molnar et al., 2006, Stempfl et al., 2002, Whitfield, 2000.9.6)

DHE3

(Mushegian et al., 1998)

GDH

(Demianchuk et al., 2024, Ratnaparkhi and Sudhakaran, 2022, Manière et al., 2020, Otto et al., 2018, Tiwari et al., 2014, Tiefenböck et al., 2010, Baltzer et al., 2009, Chapman, 2001, Chase and Kankel, 1987, Nowak and Piechowska, 1986, Caggese et al., 1982)

GLU-D

(Choudhary et al., 1992)

GLUD

(Louis, 1993.12.13, Louis, 1993.12.13, Louis, 1993.12.13, Louis, 1993.12.13, Louis, 1993.12.13, Louis, 1993.12.13)

GLUD pre-mRNA

(Louis, 1993.12.13)

Glud

(Papadopoulou and Louis, 1990)

gdh

(Maguire et al., 2015, Louis, 1997.2.17)

Name Synonyms

GLUTAMATE DEHYDROGENASE

(Alonso et al., 2005)

Glutamate dehydrogenase

(Tiwari et al., 2014, Cammarato et al., 2011, Robinson et al., 2010, Tiefenböck et al., 2010, Baltzer et al., 2009, Sun et al., 2007, Sambandan et al., 2006, Caggese et al., 1982)

NAD-dependent GDH

(Demianchuk et al., 2024)

NAD-dependent glutamate dehydrogenase

(Laayouni et al., 2007)

NADP-dependent GDH glutamate dehydrogenase

(Demianchuk et al., 2024)

glutamate dehydrogenase

(Demianchuk et al., 2024, Vedelek et al., 2023, Nowak and Piechowska, 1986, Bond and Sang, 1968)

glutamate dehydrogenase (mitochondrial)

(Talbert et al., 2015)

Secondary FlyBase IDs

Datasets (0)

Study focus (0)

Experimental Role

Project

Project Type

Title

Study result (0)

Result

Result Type

Title

External Crossreferences and Linkouts ( 84 )

Sequence Crossreferences

NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.

42832

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.

UniProt/GCRP - The gene-centric reference proteome (GCRP) provides a 1:1 mapping between genes and UniProt accessions in which a single 'canonical' isoform represents the product(s) of each protein-coding gene.

P54385

UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information

P54385

UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information

Other crossreferences

AlphaFold DB - AlphaFold provides open access to protein structure predictions for the human proteome and other key proteins of interest, to accelerate scientific research.

P54385

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0001098

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

42832/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0001098

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0001098

FlyMine - An integrated database for Drosophila genomics

FBgn0001098

InterPro - A database of protein families, domains and functional sites

KEGG Genes - Molecular building blocks of life in the genomic space.

dme:Dmel_CG5320

MARRVEL_MODEL - MARRVEL (model organism gene)

42832

Linkouts

BioGRID - A database of protein and genetic interactions.

67768

Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones

FBgn0001098

DroID - A comprehensive database of gene and protein interactions.

FBgn0001098

DRSC - Results frm RNAi screens

FBgn0001098

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

Gdh_1

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0001098

FlyCyc Genes - Genes from a BioCyc PGDB for Dmel

FBGN0001098

FlyCyc Pathways - Pathways from a BioCyc PGDB for Dmel

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG5320

Flygut - An atlas of the Drosophila adult midgut

FBgn0001098

FlyMet - A comprehensive tissue-specific metabolomics resource for Drosophila.

FBgn0001098

iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)

TC004929

KEGG Metabolic Pathways - A collection of manually drawn metabolic pathway maps representing knowledge of molecular interaction, reaction and relation networks.

KEGG Pathways - A collection of manually drawn pathway maps representing knowledge of molecular interaction, reaction and relation networks.

MIST (protein-protein) - An integrated Molecular Interaction Database

42832

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

Reactome Metabolic Pathways - An open-source, open access, manually curated and peer-reviewed metabolic pathway database.

Glutamate and glutamine metabolism

References (139)

The following external sites may use different assemblies or annotations than FlyBase.

-1.07

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