FB2024_04 , released June 25, 2024
Gene: Dmel\sav
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General Information
Symbol
Dmel\sav
Species
D. melanogaster
Name
salvador
Annotation Symbol
CG33193
Feature Type
protein_coding_gene
FlyBase ID
FBgn0053193
Gene Model Status
Current
Stock Availability
12 publicly available
Gene Summary
salvador (sav) encodes a scaffold protein involved in the Hippo signalling pathway. It promotes membrane recruitment of the kinase encoded by hpo and its assembly with the product of wts. [Date last reviewed: 2018-11-15] (FlyBase Gene Snapshot)
All Summaries
Also Known As

shar-pei

Key Links
Genomic Location
Cytogenetic map
94D10-94D10
Sequence location
Recombination map
3-77
RefSeq locus
NT_033777 REGION:23058609..23061304
Sequence
Get Decorated FASTA
Genomic Maps
Other Genome Views
The following external sites may use different assemblies or annotations than FlyBase.
NCBI
UCSC
Ensembl
PopFly
Function
Gene Ontology (GO) Annotations (23 terms)
Molecular Function (2 terms)
Terms Based on Experimental Evidence (2 terms)
CV Term
Evidence
References
enables molecular adaptor activity
inferred from genetic interaction with FLYBASE:wts; FB:FBgn0011739,FLYBASE:hpo; FB:FBgn0261456
(Harvey et al., 2003)
enables protein binding
inferred from physical interaction with UniProtKB:Q9VY77
(Das Thakur et al., 2010)
inferred from physical interaction with UniProtKB:Q9VFG8
(Yu et al., 2010)
Terms Based on Predictions or Assertions (1 term)
CV Term
Evidence
References
enables molecular adaptor activity
inferred from biological aspect of ancestor with PANTHER:PTN008717741
(GO Reference Genome Project, 2011-)
inferred from electronic annotation with InterPro:IPR030030
(InterPro Project Members, 2004-)
Biological Process (17 terms)
Terms Based on Experimental Evidence (13 terms)
CV Term
Evidence
References
involved_in hippo signaling
inferred from mutant phenotype
(Neal et al., 2022, Su et al., 2017, Aerne et al., 2015)
inferred from direct assay
(Dong et al., 2007, Huang et al., 2005)
inferred from genetic interaction with FLYBASE:wts; FB:FBgn0011739
(Wei et al., 2007)
inferred from genetic interaction with FLYBASE:wts; FB:FBgn0011739,FLYBASE:sav; FB:FBgn0053193
(Harvey et al., 2003)
involved_in imaginal disc development
inferred from mutant phenotype
(Kango-Singh et al., 2002)
involved_in Malpighian tubule development
inferred from mutant phenotype
(Zeng et al., 2010)
involved_in negative regulation of cell population proliferation
inferred from mutant phenotype
(Kango-Singh et al., 2002, Tapon et al., 2002)
involved_in negative regulation of multicellular organism growth
inferred from mutant phenotype
(Polesello et al., 2006)
involved_in positive regulation of apoptotic process
inferred from mutant phenotype
(Tapon et al., 2002)
involved_in R8 cell development
inferred from genetic interaction with FLYBASE:wts; FB:FBgn0011739,FLYBASE:kibra; FB:FBgn0262127
(Sang et al., 2021)
involved_in R8 cell fate specification
inferred from mutant phenotype
(Mikeladze-Dvali et al., 2005)
involved_in regulation of dendrite morphogenesis
inferred from genetic interaction with FLYBASE:wts; FB:FBgn0011739
(Emoto et al., 2006)
involved_in response to ionizing radiation
inferred from mutant phenotype
(Colombani et al., 2006)
involved_in retinal cell programmed cell death
inferred from mutant phenotype
(Tapon et al., 2002)
inferred from genetic interaction with FLYBASE:hid; FB:FBgn0003997
(Tapon et al., 2002)
involved_in stem cell differentiation
inferred from mutant phenotype
(Zeng et al., 2010)
involved_in stem cell proliferation
inferred from mutant phenotype
(Zeng et al., 2010)
Terms Based on Predictions or Assertions (7 terms)
CV Term
Evidence
References
involved_in apoptotic process
inferred from electronic annotation with InterPro:IPR030030
(InterPro Project Members, 2004-)
involved_in hippo signaling
inferred from biological aspect of ancestor with PANTHER:PTN008717741
(GO Reference Genome Project, 2011-)
inferred from electronic annotation with InterPro:IPR030030
(InterPro Project Members, 2004-)
traceable author statement
(Yu et al., 2010)
involved_in negative regulation of cell population proliferation
inferred from biological aspect of ancestor with PANTHER:PTN008717741
(GO Reference Genome Project, 2011-)
involved_in negative regulation of organ growth
traceable author statement
(Hafen and Stocker, 2003)
involved_in positive regulation of apoptotic process
inferred from biological aspect of ancestor with PANTHER:PTN008717741
(GO Reference Genome Project, 2011-)
involved_in regulation of cell population proliferation
inferred from electronic annotation with InterPro:IPR030030
(InterPro Project Members, 2004-)
involved_in regulation of imaginal disc growth
traceable author statement
(Lee and Orr-Weaver, 2003)
Cellular Component (4 terms)
Terms Based on Experimental Evidence (4 terms)
CV Term
Evidence
References
located_in apical part of cell
inferred from direct assay
(Yue et al., 2012)
located_in apicomedial cortex
inferred from direct assay
(Su et al., 2017)
colocalizes_with cell-cell junction
inferred from direct assay
(Su et al., 2017)
located_in cytosol
inferred from direct assay
(Yue et al., 2012)
Terms Based on Predictions or Assertions (1 term)
CV Term
Evidence
References
is_active_in cytosol
inferred from biological aspect of ancestor with PANTHER:PTN008717741
(GO Reference Genome Project, 2011-)
Gene Group (FlyBase)
Pathway (FlyBase)
Protein Family (UniProt)
-
Protein Signatures (InterPro)
Summaries
Gene Snapshot
salvador (sav) encodes a scaffold protein involved in the Hippo signalling pathway. It promotes membrane recruitment of the kinase encoded by hpo and its assembly with the product of wts. [Date last reviewed: 2018-11-15]
Pathway (FlyBase)
Hippo Signaling Pathway Core Components -
The Hippo signaling pathway is an intracellular kinase cascade in which hpo kinase in complex with sav, phosphorylates wts kinase which, in turn, phosphorylates yki transcriptional co-activator leading to its cytosolic retention. Activation of the Hippo pathway results in the down-regulation of cell proliferation and up-regulation of apoptosis, limiting tissue size. (Adapted from FBrf0224870).
Protein Function (UniProtKB)
Plays a key role in the Hippo/SWH (Sav/Wts/Hpo) signaling pathway, a signaling pathway that plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein Hippo (Hpo), in complex with its regulatory protein Salvador (Sav), phosphorylates and activates Warts (Wts) in complex with its regulatory protein Mats, which in turn phosphorylates and inactivates the Yorkie (Yki) oncoprotein. The Hippo/SWH signaling pathway inhibits the activity of the transcriptional complex formed by Scalloped (sd) and Yki and the target genes of this pathway include cyclin-E (cycE), diap1 and bantam. Required for cell cycle exit in eye imaginal disk and hid-induced apoptotic cell deaths that are part of normal retinal development. Activation of Drice in eye imaginal disk by either Hid or Rpr is almost completely blocked by Sav expression.
(UniProt, Q9VCR6)
Summary (Interactive Fly)

scaffold protein - restricts cell numbers in vivo by functioning as a dual regulator of cell proliferation and apoptosis - physically interacts with Warts - the stability of Salvador (Sav), which is believed to promote Hippo/Warts association, is crucially dependent on its binding partner Hippo

Gene Model and Products
Number of Transcripts
2
Number of Unique Polypeptides
2

Please see the JBrowse view of Dmel\sav for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)
Isoform displayed:
Pfam protein domains
InterPro name
classification
start
end
Protein Domains (via SMART)
Isoform displayed:
SMART protein domains
InterPro name
classification
start
end
Structure
Protein 3D structure   (Predicted by AlphaFold)   (AlphaFold entry Q9VCR6)

If you don't see a structure in the viewer, refresh your browser.
Model Confidence:
  • Very high (pLDDT > 90)
  • Confident (90 > pLDDT > 70)
  • Low (70 > pLDDT > 50)
  • Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Experimentally Determined Structures
Crossreferences
PDB - An information portal to biological macromolecular structures
Comments on Gene Model

Gene model reviewed during 5.48

Sequence Ontology: Class of Gene
nuclear_gene
protein_coding_gene
Transcript Data
Annotated Transcripts
Name
FlyBase ID
RefSeq ID
Length (nt)
Assoc. CDS (aa)
sav-RA
FBtr0084356
NM_176544
2235
608
sav-RB
FBtr0303509
NM_001202340
2220
603
Additional Transcript Data and Comments
Reported size (kB)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name
FlyBase ID
Predicted MW (kDa)
Length (aa)
Theoretical pI
UniProt
RefSeq ID
GenBank
sav-PA
FBpp0083749
68.1
608
9.92
Q9VCR6
NP_788721
AAF56090
sav-PB
FBpp0292543
67.5
603
9.90
A0A0B4JDB7
NP_001189269
ADV37360
Polypeptides with Identical Sequences

None of the polypeptides share 100% sequence identity.

Additional Polypeptide Data and Comments
Reported size (kDa)
Comments
External Data
Subunit Structure (UniProtKB)

Interacts with Wts via its WW domains. Interacts (via FBM motif) with Mer (via FERM domain). Interacts with Kibra. Interacts with Hpo (via SARAH domain). Interacts with jub.

(UniProt, Q9VCR6)
Post Translational Modification

Phosphorylated by Hpo.

(UniProt, Q9VCR6)
Crossreferences
InterPro - A database of protein families, domains and functional sites
PDB - An information portal to biological macromolecular structures
Linkouts
Sequences Consistent with the Gene Model
Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\sav using the Feature Mapper tool.

External Data
Crossreferences
Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
Linkouts
Expression Data
Testis-specificity index

The testis specificity index was calculated from modENCODE tissue expression data by Vedelek et al., 2018 to indicate the degree of testis enrichment compared to other tissues. Scores range from -2.52 (underrepresented) to 5.2 (very high testis bias).

0.50

Transcript Expression
in situ
Stage
Tissue/Position (including subcellular localization)
Reference
embryonic stage 1 -- 3
organism

Comment: maternally deposited

(Fisher et al., 2012)
Additional Descriptive Data
Marker for
 
Subcellular Localization
CV Term
Polypeptide Expression
mass spectroscopy
Stage
Tissue/Position (including subcellular localization)
Reference
day 5 of adulthood & male -- day 7 of adulthood & male
spermatozoon
(Wasbrough et al., 2010)
Additional Descriptive Data
Marker for
 
Subcellular Localization
CV Term
Evidence
References
located_in apical part of cell
inferred from direct assay
(Yue et al., 2012)
located_in apicomedial cortex
inferred from direct assay
(Su et al., 2017)
colocalizes_with cell-cell junction
inferred from direct assay
(Su et al., 2017)
located_in cytosol
inferred from direct assay
(Yue et al., 2012)
Expression Deduced from Reporters
High-Throughput Expression Data
Associated Tools

JBrowse - Visual display of RNA-Seq signals

View Dmel\sav in JBrowse
RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region
Bulk Downloads
RNA-Seq RPKM values for all genes
Reference
See Gelbart and Emmert, 2013 for analysis details and data files for all genes.
Developmental Proteome: Life Cycle
Developmental Proteome: Embryogenesis
External Data and Images
Linkouts
BDGP expression data - Patterns of gene expression in Drosophila embryogenesis
DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species
EMBL-EBI Single Cell Expression Atlas - Single cell expression across species
FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data
Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
Flygut - An atlas of the Drosophila adult midgut
Images
FlyBase Wiki
Image Based Resources
Alleles, Insertions, Transgenic Constructs, and Aberrations
Classical and Insertion Alleles ( 16 )
For All Classical and Insertion Alleles Show
 
Other relevant insertions
Transgenic Constructs ( 23 )
For All Alleles Carried on Transgenic Constructs Show
Transgenic constructs containing/affecting coding region of sav
Transgenic constructs containing regulatory region of sav
Aberrations (Deficiencies and Duplications) ( 3 )
Variants
Variant Molecular Consequences
Alleles Representing Disease-Implicated Variants
Phenotypes
For more details about a specific phenotype click on the relevant allele symbol.
Lethality
Allele
lethal (with Df(3R)hh)
lethal (with sav1)
lethal (with sav2)
lethal (with sav3)
lethal (with sav4)
lethal | pupal stage, with Scer\GAL4pnr-MD237
lethal | recessive
lethal - all die before end of larval stage (with Df(3R)EB6)
lethal - all die before end of larval stage (with sav1)
lethal - all die before end of larval stage (with sav3)
lethal - all die before end of larval stage | recessive
partially lethal - majority die | pupal stage, with Scer\GAL4pnr-MD237
viable, with Dcr-2UAS.cDa, Scer\GAL4elav.PLu
viable, with Scer\GAL4Mef2.PR
Other Phenotypes
Allele
abnormal body color, with Scer\GAL4pnr-MD237
abnormal cell polarity | somatic clone
abnormal neuroanatomy
abnormal neuroanatomy | adult stage | progressive | somatic clone
abnormal neuroanatomy | embryonic stage
abnormal neuroanatomy | embryonic stage | maternal effect
abnormal size | adult stage, with Scer\GAL4en.PU
abnormal size | adult stage, with Scer\GAL4ey.PH
abnormal size | adult stage, with Scer\GAL4hh-Gal4
abnormal size | adult stage, with Scer\GAL4rn.PU
abnormal size | adult stage | somatic clone
abnormal stress response | somatic clone
decreased body size, with Scer\GAL4da.G32
decreased occurrence of cell division | larval stage, with Scer\GAL4en.PU
increased body size | somatic clone
increased cell number | P-stage | somatic clone - tissue specific
increased cell number | pupal stage | somatic clone
increased cell number | somatic clone
increased occurrence of cell division | adult stage | cell autonomous | somatic clone
increased occurrence of cell division | adult stage | somatic clone
increased size | adult stage | somatic clone - tissue specific
some die during larval stage (with Df(3R)EB6)
some die during larval stage (with sav1)
some die during larval stage (with sav3)
some die during larval stage | recessive
visible, with Scer\GAL4pnr-MD237
visible | adult stage | somatic clone - tissue specific
visible | recessive
visible | somatic clone
Phenotype manifest in
Allele
adult head | somatic clone
adult head | somatic clone - tissue specific
adult Malpighian tubule | somatic clone
adult thorax | somatic clone
autophagosome | progressive | somatic clone
cell cycle | somatic clone
chaeta | increased number, with Scer\GAL4pnr-MD237
dorsal multidendritic neuron ddaC | somatic clone & dendritic tree
embryonic neuroblast | maternal effect
eye
eye, with Scer\GAL4ey.PH
eye | somatic clone
eye | somatic clone - tissue specific
eye disc | cell autonomous | somatic clone
eye disc | somatic clone
eye photoreceptor cell | progressive | somatic clone
eye photoreceptor cell | somatic clone
haltere | somatic clone
interommatidial bristle | increased number
interommatidial cell | P-stage | somatic clone - tissue specific
intestinal stem cell | somatic clone
larval RP2 motor neuron | embryonic stage
mesothoracic tergum, with Scer\GAL4pnr-MD237
mesothoracic tergum | somatic clone
mitotic spindle | embryonic stage | maternal effect
ommatidial precursor cluster | somatic clone
photoreceptor cell R8 of pale ommatidium
photoreceptor cell R8 of yellow ommatidium
pigment cell | ectopic | somatic clone
pigment cell | somatic clone | increased number
trichogen cell, with Scer\GAL4pnr-MD237
wing, with Scer\GAL4Bx-MS1096
wing, with Scer\GAL4en.PU
wing, with Scer\GAL4rn.PU
wing | posterior, with Scer\GAL4hh-Gal4
wing disc | somatic clone
wing disc posterior compartment, with Scer\GAL4en.PU
Orthologs
Downloads
Download All DIOPT Orthologs
Human Orthologs (via DIOPT v9.1)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Source
Compara
Domainoid
eggNOG
Hieranoid
Homologene
Inparanoid
OMA
OrthoDB
OrthoFinder
OrthoInspector
orthoMCL
Panther
Phylome
SonicParanoid
Alignment
Complementation?
Transgene?
Homo sapiens (Human) (7)
Hsap\SAV1
9 of 14
Yes
Yes
1  
Hsap\MAGI3
2 of 14
No
No
Hsap\ITCH
1 of 14
No
No
Hsap\MAGI1
1 of 14
No
No
Hsap\MAGI2
1 of 14
No
No
Hsap\MAGIX
1 of 14
No
No
Hsap\NEDD4
1 of 14
No
No
Model Organism Orthologs (via DIOPT v9.1)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Source
Compara
Domainoid
eggNOG
Hieranoid
Homologene
Inparanoid
OMA
OrthoDB
OrthoFinder
OrthoInspector
orthoMCL
Panther
Phylome
SonicParanoid
Alignment
Complementation?
Transgene?
Rattus norvegicus (Norway rat) (7)
Rnor\Sav1
9 of 14
Yes
Yes
Rnor\Magi3
2 of 14
No
No
Rnor\Itch
1 of 14
No
No
Rnor\Magi1
1 of 14
No
No
Rnor\Magi2
1 of 14
No
No
Rnor\Magix
1 of 14
No
No
Rnor\Smurf1
1 of 14
No
No
Mus musculus (laboratory mouse) (8)
Mmus\Sav1
9 of 14
Yes
Yes
Mmus\Itch
1 of 14
No
No
Mmus\Magi1
1 of 14
No
No
Mmus\Magi2
1 of 14
No
No
Mmus\Magi3
1 of 14
No
No
Mmus\Magix
1 of 14
No
No
Mmus\Smurf1
1 of 14
No
No
Mmus\Yap1
1 of 14
No
No
Xenopus tropicalis (Western clawed frog) (4)
Xtro\sav1
6 of 13
Yes
Yes
Xtro\magi3
2 of 13
No
No
Xtro\magi1
1 of 13
No
No
Xtro\magi2
1 of 13
No
No
Danio rerio (Zebrafish) (12)
Drer\sav1
9 of 14
Yes
Yes
Drer\LOC564220
2 of 14
No
No
Drer\magi3a
2 of 14
No
No
Drer\magixa
2 of 14
No
No
Drer\LOC556872
1 of 14
No
No
Drer\magi1a
1 of 14
No
No
Drer\magi1b
1 of 14
No
No
Drer\magi2a
1 of 14
No
No
Drer\magi2b
1 of 14
No
No
Drer\smurf2
1 of 14
No
No
Drer\stxbp4
1 of 14
No
No
Drer\wwox
1 of 14
No
No
Caenorhabditis elegans (Nematode, roundworm) (2)
Cele\sav-1
8 of 14
Yes
Yes
Cele\magi-1
1 of 14
No
No
Anopheles gambiae (African malaria mosquito) (2)
Agam\AgaP_AGAP004628
9 of 12
Yes
Yes
Agam\AgaP_AGAP010754
1 of 12
No
No
Arabidopsis thaliana (thale-cress) (0)
Saccharomyces cerevisiae (Brewer's yeast) (0)
Schizosaccharomyces pombe (Fission yeast) (0)
Escherichia coli (enterobacterium) (0)
Other Organism Orthologs (via OrthoDB)
Data provided directly from OrthoDB:sav. Refer to their site for version information.
Paralogs
Downloads
Download All DIOPT Paralogs
Paralogs (via DIOPT v9.1)
Gene Symbol
Score
Source
Compara
Domainoid
eggNOG
Homologene
Inparanoid
OMA
OrthoDB
OrthoFinder
OrthoInspector
orthoMCL
Panther
Phylome
SonicParanoid
Alignment
Drosophila melanogaster (Fruit fly) (1)
Magi
2 of 13
Human Disease Associations
FlyBase Human Disease Model Reports
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 1 )
Allele
Disease
Evidence
References
sav3
model of  cancer
CEA
(Liu et al., 2022, Yang et al., 2022)
model of  kidney cancer
CEA
(Zeng et al., 2010)
Potential Models Based on Orthology ( 0 )
Human Ortholog
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 1 )
Allele
Disease
Interaction
References
sav3
model of  cancer
is ameliorated by Ptp61FΔ
(Liu et al., 2022)
is ameliorated by Xbp1UAS.s.Tag:V5
(Yang et al., 2022)
is ameliorated by Ire1UAS.Tag:V5
(Yang et al., 2022)
exacerbates  dentatorubral-pallidoluysian atrophy
modeled by Gug75QN.UAS
(Napoletano et al., 2011)
Disease Associations of Human Orthologs (via DIOPT v9.1 and OMIM)
Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.
Homo sapiens (Human)
Gene name
Score
OMIM
OMIM Phenotype
DO term
Complementation?
Transgene?
SAV1; salvador family WW domain containing protein 1
9 of 14
607203
       
      Functional Complementation Data
      Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.
      Interactions
      Summary of Physical Interactions
      esyN Network Diagram
      Show neighbor-neighbor interactions:
      Show/hide secondary interactors 
      (data from AllianceMine provided by esyN)
      Select Layout:
      Legend:
      Protein
      RNA
      Selected Interactor(s)
      Other Interaction Browsers
      View in FlyBase Interactions Browser   View in MIST tool (external link)
      Please see the Physical Interaction reports below for full details
      protein-protein
      Physical Interaction
      Assay
      References
      sav
      mass spectrometry studies of complexes, pull down, molecular weight estimation by staining, fluorescent resonance energy transfer, fluorescence microscopy, anti tag coimmunoprecipitation, anti tag western blot
      (Cairns et al., 2020, Yue et al., 2012)
      sav - Arf1
      anti tag coimmunoprecipitation, peptide massfingerprinting
      (Kwon et al., 2013)
      sav - AsnRS
      anti tag coimmunoprecipitation, western blot, anti tag western blot
      (Yeom et al., 2020)
      sav - CCT3
      anti tag coimmunoprecipitation, peptide massfingerprinting
      (Kwon et al., 2013)
      sav - CG9581
      anti tag coimmunoprecipitation, peptide massfingerprinting
      (Kwon et al., 2013)
      sav - CtsL1
      anti tag coimmunoprecipitation, peptide massfingerprinting
      (Kwon et al., 2013)
      sav - ERp44
      anti tag coimmunoprecipitation, peptide massfingerprinting
      (Kwon et al., 2013)
      sav - Herc4
      anti tag coimmunoprecipitation, anti tag western blot
      (Aerne et al., 2015)
      sav - Mer
      anti tag coimmunoprecipitation, western blot, two hybrid, anti tag western blot
      (Wehr et al., 2013, Yu et al., 2010)
      sav - Nup50
      anti tag coimmunoprecipitation, peptide massfingerprinting
      (Kwon et al., 2013)
      sav - Ran
      anti tag coimmunoprecipitation, peptide massfingerprinting
      (Kwon et al., 2013)
      sav - Rassf
      pull down, molecular weight estimation by staining
      (Cairns et al., 2020)
      sav - Sik2
      anti tag coimmunoprecipitation, anti tag western blot
      (Wehr et al., 2013)
      sav - Sik3
      anti tag coimmunoprecipitation, anti tag western blot
      (Wehr et al., 2013)
      sav - drk
      anti tag coimmunoprecipitation, peptide massfingerprinting
      (Kwon et al., 2013)
      sav - ed
      anti tag coimmunoprecipitation, anti tag western blot, fluorescent resonance energy transfer, fluorescence microscopy
      (Yue et al., 2012)
      sav - ex
      anti tag coimmunoprecipitation, anti tag western blot
      (Yu et al., 2010)
      sav - hpo
      anti tag coimmunoprecipitation, anti tag western blot, enzymatic study, autoradiography, bimolecular fluorescence complementation, fluorescence microscopy, anti bait coimmunoprecipitation, western blot, peptide massfingerprinting, two hybrid, pull down, tandem affinity purification, molecular sieving, molecular weight estimation by staining, coimmunoprecipitation, protein cross-linking with a bifunctional reagent, molecular weight, x-ray crystallography, mass spectrometry studies of complexes
      (Bonello et al., 2023, Cairns et al., 2020, Yeom et al., 2020, Cairns et al., 2018, Aerne et al., 2015, Dent et al., 2015, Dong et al., 2015, Li et al., 2015, Huang et al., 2013, Kwon et al., 2013, Wehr et al., 2013, Yin et al., 2013, Jin et al., 2012, Reddy and Irvine, 2011, Ribeiro et al., 2010, Yu et al., 2010, Polesello et al., 2006, Harvey et al., 2003, Jia et al., 2003, Pantalacci et al., 2003, Udan et al., 2003, Wu et al., 2003)
      sav - jub
      anti tag coimmunoprecipitation, anti tag western blot
      (Reddy and Irvine, 2013)
      sav - kibra
      anti tag coimmunoprecipitation, anti tag western blot, two hybrid, western blot
      (Yu et al., 2010)
      sav - l(1)G0289
      anti tag coimmunoprecipitation, peptide massfingerprinting
      (Kwon et al., 2013)
      sav - lig
      coimmunoprecipitation, peptide massfingerprinting, anti tag coimmunoprecipitation, anti tag western blot, western blot
      (Dong et al., 2015)
      sav - par-1
      anti tag coimmunoprecipitation, anti tag western blot
      (Huang et al., 2013)
      sav - sta
      anti tag coimmunoprecipitation, peptide massfingerprinting
      (Kwon et al., 2013)
      sav - wts
      pull down, anti tag western blot
      (Tapon et al., 2002)
      Summary of Genetic Interactions
      esyN Network Diagram
      Show/hide secondary interactors 
      (data from AllianceMine provided by esyN)
      esyN Network Key:
      Suppression
      Enhancement
      Other Interaction Browsers
      View in FlyBase Interactions Browser   View in MIST tool (external link)
      Please look at the allele data for full details of the genetic interactions
      Starting gene(s)
      Interaction type
      Interacting gene(s)
      Reference
      sav
      suppressible
      AsnRS
      (Yeom et al., 2020)
      sav|wts
      suppressible
      BEAF-32
      (Jukam et al., 2016)
      sav
      enhanceable
      CtBP
      (Vissers et al., 2020)
      sav
      suppressible
      Herc4
      (Aerne et al., 2015)
      sav
      enhanceable
      Mer
      (Yin et al., 2013)
      sav
      suppressible
      mir-ban
      (Huang et al., 2014)
      sav
      enhanceable
      Rassf
      (Polesello et al., 2006)
      sav|wts
      suppressible
      Rassf
      (Polesello et al., 2006)
      sav
      suppressible
      sd
      (Zhang et al., 2008)
      wts|sav
      enhanceable
      Tao
      (Poon et al., 2011)
      sav
      suppressible
      Tgi
      (Guo et al., 2013)
      Starting gene(s)
      Interaction type
      Interacting gene(s)
      Reference
      AsnRS
      suppressible
      sav
      (Yeom et al., 2020)
      BEAF-32
      suppressible
      sav|wts
      (Jukam et al., 2016)
      CtBP
      enhanceable
      sav
      (Vissers et al., 2020)
      ed
      suppressible
      sav
      (Yue et al., 2012)
      Gug
      enhanceable
      sav
      (Napoletano et al., 2011)
      hid
      suppressible
      sav
      (Tapon et al., 2002)
      hpo
      enhanceable
      sav
      (Wu et al., 2003)
      kibra
      suppressible
      sav
      (Yu et al., 2010)
      Magi
      enhanceable
      sav
      (Zaessinger et al., 2015)
      Mer
      enhanceable
      sav
      (Yin et al., 2013)
      p53
      suppressible
      sav
      (Colombani et al., 2006)
      External Data
      Subunit Structure (UniProtKB)
      Interacts with Wts via its WW domains. Interacts (via FBM motif) with Mer (via FERM domain). Interacts with Kibra. Interacts with Hpo (via SARAH domain). Interacts with jub.
      (UniProt, Q9VCR6 )
      Linkouts
      BioGRID - A database of protein and genetic interactions.
      DroID - A comprehensive database of gene and protein interactions.
      MIST (genetic) - An integrated Molecular Interaction Database
      MIST (protein-protein) - An integrated Molecular Interaction Database
      Pathways
      Signaling Pathways (FlyBase)
      Hippo Signaling Pathway Core Components -
      The Hippo signaling pathway is an intracellular kinase cascade in which hpo kinase in complex with sav, phosphorylates wts kinase which, in turn, phosphorylates yki transcriptional co-activator leading to its cytosolic retention. Activation of the Hippo pathway results in the down-regulation of cell proliferation and up-regulation of apoptosis, limiting tissue size. (Adapted from FBrf0224870).
      Metabolic Pathways
      External Data
      Linkouts
      KEGG Pathways - A collection of manually drawn pathway maps representing knowledge of molecular interaction, reaction and relation networks.
      Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.
      SignaLink - A signaling pathway resource with multi-layered regulatory networks.
      Genomic Location and Detailed Mapping Data
      Chromosome (arm)
      3R
      Recombination map
      3-77
      Cytogenetic map
      94D10-94D10
      Sequence location
      FlyBase Computed Cytological Location
      Cytogenetic map
      Evidence for location
      94D10-94D10
      Limits computationally determined from genome sequence between P{lacW}GclmL0580 and P{EP}hhEP3521
      Experimentally Determined Cytological Location
      Cytogenetic map
      Notes
      References
      Experimentally Determined Recombination Data
      Location

      3-77

      (FlyBase, 2016)

      3-79.3

      (Comeron et al., 2012)

      3-

      (Tapon and Hariharan, 1999)
      Left of (cM)
      (Kango-Singh et al., 2002)
      Right of (cM)
      (Kango-Singh et al., 2002)
      Notes

      Located on the right arm of chromosome 3

      (Tapon and Hariharan, 1999)
      Stocks and Reagents
      Stocks (12)
      Genomic Clones (16)
       

      Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

      cDNA Clones (116)
       

      Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see JBrowse for alignment of the cDNAs and ESTs to the gene model.

      cDNA clones, fully sequenced
      BDGP DGC clones
      Other clones
        Drosophila Genomics Resource Center cDNA clones

        For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

        cDNA Clones, End Sequenced (ESTs)
        BDGP DGC clones
        Other clones
        RNAi and Array Information
        Linkouts
        DRSC - Results frm RNAi screens
        Antibody Information
        Laboratory Generated Antibodies
         

        polyclonal

        (Yue et al., 2012)
        Commercially Available Antibodies
         
        Cell Line Information
        Publicly Available Cell Lines
         
          Other Stable Cell Lines
           
            Other Comments

            sav acts cell autonomously in class IV neurons.

            (Emoto et al., 2006)

            dsRNA has been made from templates generated with primers directed against this gene.

            (Polesello et al., 2006)

            sav is required for the proper termination of cell proliferation during imaginal disc development.

            (Kango-Singh et al., 2002)

            sav promotes both cell cycle exit and apoptosis.

            (Tapon et al., 2002)

            Identification: Isolated as a mutation that causes overgrowth in homozygous clones in the eye.

            (Tapon and Hariharan, 1999)

            Mutations in sav abolish developmentally controlled programmed cell death in homozygous clones in the eye.

            (Tapon and Hariharan, 1999)
            Relationship to Other Genes
            Source for database merge of

            Source for merge of: salvador CG13831

            (Tapon, 2002.7.12)

            Source for merge of: CG13831 CG13832

            (FlyBase, 1996-)

            Source for merge of: sav anon-WO0172774.152

            (FlyBase, 1996-)
            Additional comments

            Annotations CG13831, CG13832 merged as CG33193 in release 3 of the genome annotation.

            (FlyBase Genome Annotators, 2002-2003)

            Source for merge of CG13831 CG13832 was a shared cDNA ( date:010720 ).

            (FlyBase, 1996-)

            Source for merge of sav anon-WO0172774.152 was sequence comparison ( date:051113 ).

            (FlyBase, 1996-)
            Nomenclature History
            Source for database identify of
            Nomenclature comments
            Etymology

            The gene is called "shar-pei" because of the mutant folded cuticle phenotype in the head, which resembles the folded skin of Shar-pei dogs.

            (Kango-Singh et al., 2002)
            Synonyms and Secondary IDs (14)
            Reported As
            Symbol Synonym
            CG13831
            (Kango-Singh et al., 2002)
            CG13832
            CG33193
            (Ni et al., 2010.12.1, Keleman et al., 2009.8.5, Perkins et al., 2009, Formstecher et al., 2005, Giot et al., 2003)
            SAV
            (Gonzalez, 2013, Grifoni et al., 2013, Harvey and Hariharan, 2012)
            SAV1
            (Wang et al., 2020)
            Salvador
            (Baker, 2020)
            Salvador/Shar-pei
            (Vidal and Cagan, 2006)
            Sav
            (Bonello et al., 2023, Cho and Jiang, 2021, Mohajan et al., 2021, Gou et al., 2020, Mohammadi et al., 2020, Sahu and Mondal, 2020, van Soldt and Cardoso, 2020, Yeom et al., 2020, Gokhale and Pfleger, 2019, Meltzer, 2019-, Meltzer et al., 2019, Bae and Luo, 2018, Cairns et al., 2018, Elbediwy and Thompson, 2018, Enomoto et al., 2018, Fletcher et al., 2018, Fulford et al., 2018, Kim and Jho, 2018, Jang et al., 2017, Richardson and Portela, 2017, Tue et al., 2017, Becker et al., 2016, Chung et al., 2016, Fallahi et al., 2016, Jahanshahi et al., 2016, Meng et al., 2016, Yadav et al., 2016, Aerne et al., 2015, Bae et al., 2015, Cao et al., 2015, Dent et al., 2015, Irvine and Harvey, 2015, Li et al., 2015, Thompson and Sahai, 2015, Wang et al., 2015, Sadeqzadeh et al., 2014, Andersen et al., 2013, Degoutin et al., 2013, Deng et al., 2013, Enderle and McNeill, 2013, Huang et al., 2013, Johnston, 2013, Kwon et al., 2013, Matsui and Lai, 2013, Puram and Bonni, 2013, Rister et al., 2013, Wehr et al., 2013, Wu and Wu, 2013, Yin et al., 2013, Fausti et al., 2012, Hergovich and Hemmings, 2012, Jin et al., 2012, Liu et al., 2012, Chan et al., 2011, Genevet and Tapon, 2011, Halder and Johnson, 2011, Laprise, 2011, Reddy and Irvine, 2011, Salah and Aqeilan, 2011, Zhang et al., 2011, Zhao et al., 2011, Ribeiro et al., 2010, Yu et al., 2010, Yu et al., 2010, Zeng et al., 2010, Oh and Irvine, 2009, Zhang et al., 2009, Jukam and Claude, 2008, Oh and Irvine, 2008, Zhai et al., 2008, Dong et al., 2007, Jukam and Desplan, 2007, Polesello and Tapon, 2007, Hariharan, 2006, Polesello et al., 2006, Formstecher et al., 2005)
            Sav1
            (van Soldt and Cardoso, 2020)
            anon-WO0172774.152
            sav
            (Kroeger et al., 2024, Gao et al., 2022, Li et al., 2022, Liu et al., 2022, Dong et al., 2021, Gogia et al., 2021, Gong et al., 2021, Lindsey et al., 2021, Sang et al., 2021, Tokamov et al., 2021, Cairns et al., 2020, Morata and Calleja, 2020, Vissers et al., 2020, Chang et al., 2019, Fahey-Lozano et al., 2019, Snigdha et al., 2019, Albert et al., 2018, Baillon et al., 2018, Gene Disruption Project members, 2018-, Gou et al., 2018, Yu and Pan, 2018, Baker, 2017, Su et al., 2017, Transgenic RNAi Project members, 2017-, Jahanshahi et al., 2016, Jukam et al., 2016, Liu et al., 2016, Mao et al., 2016, Aerne et al., 2015, Dent et al., 2015, Doggett et al., 2015, Dong et al., 2015, Keder et al., 2015, Sun et al., 2015, Wang and Baker, 2015, Zaessinger et al., 2015, Chen et al., 2014, Huang and Kalderon, 2014, Huang et al., 2014, Rauskolb et al., 2014, Robbins et al., 2014, Tipping and Perrimon, 2014, Deng et al., 2013, Guo et al., 2013, Huang et al., 2013, Jukam et al., 2013, Jukam et al., 2013, Kwon et al., 2013, Kwon et al., 2013, Yin et al., 2013, Hafezi et al., 2012, Japanese National Institute of Genetics, 2012.5.21, Poon et al., 2012, Verghese et al., 2012, Yue et al., 2012, Bao et al., 2011, Fernández et al., 2011, Napoletano et al., 2011, Ohsawa et al., 2011, Poon et al., 2011, Baumgartner et al., 2010, Das Thakur et al., 2010, Grzeschik et al., 2010, Milton et al., 2010, Robinson et al., 2010, Shaw et al., 2010, Wasbrough et al., 2010, Parrish et al., 2009, Rhiner et al., 2009, Dutta and Baehrecke, 2008, Sun et al., 2008, Yu et al., 2008, Zhang et al., 2008, Menut et al., 2007, Parrish et al., 2007, Polesello and Tapon, 2007, Tyler and Baker, 2007, Tyler et al., 2007, Wei et al., 2007, Bennett and Harvey, 2006, Cho et al., 2006, Colombani et al., 2006, Edgar, 2006, Emoto et al., 2006, Hamaratoglu et al., 2006, Polesello et al., 2006, Firth and Baker, 2005, Mikeladze-Dvali et al., 2005, Pantalacci, 2003)
            shar-pei
            (Hay and Guo, 2003, Kango-Singh et al., 2003)
            Name Synonyms
            Salvador
            (Gou et al., 2023, Chen et al., 2020, Cui et al., 2020, Finegan and Bergstralh, 2020, Wang et al., 2020, Mirzoyan et al., 2019, Cairns et al., 2018, Kim and Jho, 2018, Moon et al., 2018, Becker et al., 2016, Sadeqzadeh et al., 2014, Degoutin et al., 2013, Jukam et al., 2013, Levayer and Moreno, 2013, Reddy and Irvine, 2013, Wehr et al., 2013, Wu and Wu, 2013, Yang and Hata, 2013, Fausti et al., 2012, Jin et al., 2012, Verghese et al., 2012, Bao et al., 2011, Halder and Johnson, 2011, Laprise, 2011, Reddy and Irvine, 2011, Das Thakur et al., 2010, Grzeschik et al., 2010, Ribeiro et al., 2010, Robinson et al., 2010, Yu et al., 2010, Deng et al., 2008, Dutta and Baehrecke, 2008, Yu et al., 2008, Jukam and Desplan, 2007, Polesello and Tapon, 2007, Colombani et al., 2006, Emoto et al., 2006, Hariharan, 2006, Polesello et al., 2006, Silva et al., 2006)
            salvador
            (Kuleesha et al., 2016, Harvey et al., 2013, Huang et al., 2013, Turner et al., 2011, Milton et al., 2010, Parrish et al., 2009, Rhiner et al., 2009, Zhang et al., 2009, Sun et al., 2008, Xu and Bergmann, 2008, Menut et al., 2007, Bennett and Harvey, 2006, Cho et al., 2006, Bateman and McNeill, 2005, Tapon et al., 2002)
            salvador/shar-pei
            (Bergmann and Lane, 2003)
            Secondary FlyBase IDs
            • FBgn0026180
            • FBgn0039046
            • FBgn0039047
            • FBgn0046446
            Datasets (1)
            Study focus (1)
            Experimental Role
            Project
            Project Type
            Title
            • bait_protein
            Hippo_Pathway_interaction_map
            Interaction map generated by purification of Hippo pathway factors, with identification of copurifying proteins by mass spectrometry.
            Study result (0)
            Result
            Result Type
            Title
            External Crossreferences and Linkouts ( 51 )
            Sequence Crossreferences
            NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.
            GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.
            GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.
            RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.
            UniProt/GCRP - The gene-centric reference proteome (GCRP) provides a 1:1 mapping between genes and UniProt accessions in which a single 'canonical' isoform represents the product(s) of each protein-coding gene.
            UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information
            UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information
            Other crossreferences
            AlphaFold DB - AlphaFold provides open access to protein structure predictions for the human proteome and other key proteins of interest, to accelerate scientific research.
            BDGP expression data - Patterns of gene expression in Drosophila embryogenesis
            DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species
            EMBL-EBI Single Cell Expression Atlas - Single cell expression across species
            FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data
            FlyMine - An integrated database for Drosophila genomics
            InterPro - A database of protein families, domains and functional sites
            KEGG Genes - Molecular building blocks of life in the genomic space.
            MARRVEL_MODEL - MARRVEL (model organism gene)
            PDB - An information portal to biological macromolecular structures
            Linkouts
            BioGRID - A database of protein and genetic interactions.
            Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones
            DroID - A comprehensive database of gene and protein interactions.
            DRSC - Results frm RNAi screens
            Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
            FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
            FlyCyc Genes - Genes from a BioCyc PGDB for Dmel
            Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
            Flygut - An atlas of the Drosophila adult midgut
            iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)
            Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution
            KEGG Pathways - A collection of manually drawn pathway maps representing knowledge of molecular interaction, reaction and relation networks.
            MIST (genetic) - An integrated Molecular Interaction Database
            MIST (protein-protein) - An integrated Molecular Interaction Database
            Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.
            SignaLink - A signaling pathway resource with multi-layered regulatory networks.
            References (262)