The Weizmann-KO collection (FBlc0003496) consists of a set of transgenic constructs for sgRNA-targeted gene mutation. Each transgenic construct contains two short guide RNA (sgRNA) sequences that both target the coding region of a gene immediately downstream of the translation initiation site. The sgRNAs are expressed under the control of a ubiquitous promoter. When crossed to a stock that contains a source of Cas9, cleavage and mutagenesis of the targeted gene is induced, resulting in a small deletion or insertion mutation.
The 'Associated Files' for this personal communication consist of .xlsx files that were submitted to FlyBase at the time that each set of the fly stocks was sent to the Bloomington Drosophila Stock Center, for those stocks that were not described in the original publication for this collection (Meltzer et al., 2019, Nat. Commun. 10(1): 2113, FBrf0242300).