FlyBase Allele Report: Dmel\TBPH[RNAi.UAS.cUa]

General Information

Symbol

Dmel\TBPH^RNAi.UAS.cUa

Species

D. melanogaster

Name

Saccharomyces cerevisiae UAS construct a of Unknown

FlyBase ID

FBal0299765

Feature type

allele

Associated gene

Dmel\TBPH

Associated Insertion(s)

Carried in Construct

P{UAS-TBPH.RNAi.U}

Key Links

Transgenic product class

RNAi_reagent

(Ihara et al., 2013)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Ihara et al., 2013)

Mutagen

in vitro construct

(Ihara et al., 2013)

Progenitor genotype

Carried in construct

P{UAS-TBPH.RNAi.U}

Cytology

Description

UAS regulatory sequences drive expression of a TBPH RNAi line.

(Ihara et al., 2013)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

TBPH

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Disease

Evidence

References

model of amyotrophic lateral sclerosis type 10

CEA

(Lehmkuhl et al., 2021)

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

model of amyotrophic lateral sclerosis type 10

is ameliorated by dlp^UAS.cBa

(Lehmkuhl et al., 2021)

is ameliorated by dlp^GLC01658

(Lehmkuhl et al., 2021)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal locomotor behavior | adult stage, with Scer\GAL4^elav.PU, TBPH^Δ23

(Appocher et al., 2017)

abnormal neuroanatomy, with Scer\GAL4^GMR.PU

(Ihara et al., 2013)

Phenotype Manifest In

retina, with Scer\GAL4^GMR.PU

(Ihara et al., 2013)

Detailed Description

Statement

Reference

TBPH^Δ23/+ adult flies also expressing TBPH^{dsRNA.Scer\UAS.cUa} (together with UAS-Dicer2 to enhance RNAi efficiency) under the control of Scer\GAL4^elav.PU display significant locomotor deficit in climbing assays.

(Appocher et al., 2017)

Motor neuron clones expressing TBPH^{dsRNA.Scer\UAS.cUa} under the control of Scer\GAL4^VGlut-OK371 do not exhibit any observable degeneration.

(Sreedharan et al., 2015)

The retina of flies expressing TBPH^{dsRNA.Scer\UAS.cUa} under the control of Scer\GAL4^GMR.PU exhibit scattered vacuolar degeneration.

(Ihara et al., 2013)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Scer\GAL4^elav.PU, TBPH^Δ23, TBPH^RNAi.UAS.cUa has abnormal locomotor behavior | adult stage phenotype, enhanceable by CG42458^GD16040, Scer\GAL4^elav.PU

(Appocher et al., 2017)

Scer\GAL4^elav.PU, TBPH^Δ23, TBPH^RNAi.UAS.cUa has abnormal locomotor behavior | adult stage phenotype, enhanceable by CG42458^KK113285, Scer\GAL4^elav.PU

(Appocher et al., 2017)

Scer\GAL4^elav.PU, TBPH^Δ23, TBPH^RNAi.UAS.cUa has abnormal locomotor behavior | adult stage phenotype, enhanceable by Scer\GAL4^elav.PU/heph^KK108057

(Appocher et al., 2017)

Scer\GAL4^elav.PU, TBPH^Δ23, TBPH^RNAi.UAS.cUa has abnormal locomotor behavior | adult stage phenotype, enhanceable by HnRNP-K^GD1382, Scer\GAL4^elav.PU

(Appocher et al., 2017)

Scer\GAL4^elav.PU, TBPH^Δ23, TBPH^RNAi.UAS.cUa has abnormal locomotor behavior | adult stage phenotype, enhanceable by HnRNP-K^KK106619, Scer\GAL4^elav.PU

(Appocher et al., 2017)

Scer\GAL4^elav.PU, TBPH^Δ23, TBPH^RNAi.UAS.cUa has abnormal locomotor behavior | adult stage phenotype, enhanceable by Scer\GAL4^elav.PU/sm^GD12545

(Appocher et al., 2017)

Scer\GAL4^elav.PU, TBPH^Δ23, TBPH^RNAi.UAS.cUa has abnormal locomotor behavior | adult stage phenotype, enhanceable by sm^KK108588/Scer\GAL4^elav.PU

(Appocher et al., 2017)

Scer\GAL4^elav.PU, TBPH^Δ23, TBPH^RNAi.UAS.cUa has abnormal locomotor behavior | adult stage phenotype, enhanceable by sqd^GD8593/Scer\GAL4^elav.PU

(Appocher et al., 2017)

Scer\GAL4^elav.PU, TBPH^Δ23, TBPH^RNAi.UAS.cUa has abnormal locomotor behavior | adult stage phenotype, enhanceable by Scer\GAL4^elav.PU/rump^GD14202

(Appocher et al., 2017)

Scer\GAL4^elav.PU, TBPH^Δ23, TBPH^RNAi.UAS.cUa has abnormal locomotor behavior | adult stage phenotype, enhanceable by Scer\GAL4^elav.PU/rump^KK102889

(Appocher et al., 2017)

Scer\GAL4^elav.PU, TBPH^Δ23, TBPH^RNAi.UAS.cUa has abnormal locomotor behavior | adult stage phenotype, enhanceable by CG30122^KK100713, Scer\GAL4^elav.PU

(Appocher et al., 2017)

Scer\GAL4^elav.PU, TBPH^Δ23, TBPH^RNAi.UAS.cUa has abnormal locomotor behavior | adult stage phenotype, enhanceable by Hrb87F^KK108162, Scer\GAL4^elav.PU

(Appocher et al., 2017)

Scer\GAL4^elav.PU, TBPH^Δ23, TBPH^RNAi.UAS.cUa has abnormal locomotor behavior | adult stage phenotype, enhanceable by Scer\GAL4^elav.PU/glo^GD12082

(Appocher et al., 2017)

Scer\GAL4^elav.PU, TBPH^Δ23, TBPH^RNAi.UAS.cUa has abnormal locomotor behavior | adult stage phenotype, enhanceable by glo^KK108457/Scer\GAL4^elav.PU

(Appocher et al., 2017)

Scer\GAL4^elav.PU, TBPH^Δ23, TBPH^RNAi.UAS.cUa has abnormal locomotor behavior | adult stage phenotype, enhanceable by Scer\GAL4^elav.PU/heph^GD10126

(Appocher et al., 2017)

Enhancer of

Statement

Reference

TBPH^RNAi.UAS.cUa, TBPH[+], TBPH^Δ23, Scer\GAL4^elav.PU is an enhancer of abnormal locomotor behavior | adult stage phenotype of HnRNP-K^GD1382, Scer\GAL4^elav.PU

(Appocher et al., 2017)

TBPH^RNAi.UAS.cUa, TBPH[+], TBPH^Δ23, Scer\GAL4^elav.PU is an enhancer of abnormal locomotor behavior | adult stage phenotype of HnRNP-K^KK106619, Scer\GAL4^elav.PU

(Appocher et al., 2017)

TBPH^RNAi.UAS.cUa, Scer\GAL4^elav.PU, TBPH[+], TBPH^Δ23 is an enhancer of abnormal locomotor behavior | adult stage phenotype of Scer\GAL4^elav.PU, heph^GD10126

(Appocher et al., 2017)

TBPH^RNAi.UAS.cUa, Scer\GAL4^elav.PU, TBPH[+], TBPH^Δ23 is an enhancer of abnormal locomotor behavior | adult stage phenotype of Scer\GAL4^elav.PU, heph^KK108057

(Appocher et al., 2017)

TBPH^RNAi.UAS.cUa, Scer\GAL4^elav.PU, TBPH[+], TBPH^Δ23 is an enhancer of abnormal locomotor behavior | adult stage phenotype of Scer\GAL4^elav.PU, sm^GD12545

(Appocher et al., 2017)

TBPH^RNAi.UAS.cUa, Scer\GAL4^elav.PU, TBPH[+], TBPH^Δ23 is an enhancer of abnormal locomotor behavior | adult stage phenotype of Scer\GAL4^elav.PU, sm^KK108588

(Appocher et al., 2017)

TBPH^RNAi.UAS.cUa, TBPH[+], TBPH^Δ23, Scer\GAL4^elav.PU is an enhancer of abnormal locomotor behavior | adult stage phenotype of Hrb87F^KK108162, Scer\GAL4^elav.PU

(Appocher et al., 2017)

TBPH^RNAi.UAS.cUa, Scer\GAL4^elav.PU, TBPH[+], TBPH^Δ23 is an enhancer of abnormal locomotor behavior | adult stage phenotype of Scer\GAL4^elav.PU, glo^GD12082

(Appocher et al., 2017)

TBPH^RNAi.UAS.cUa, Scer\GAL4^elav.PU, TBPH[+], TBPH^Δ23 is an enhancer of abnormal locomotor behavior | adult stage phenotype of Scer\GAL4^elav.PU, glo^KK108457

(Appocher et al., 2017)

Other

Statement

Reference

Hrb27C^GD6964, Scer\GAL4^elav.PU, TBPH[+]/TBPH^Δ23, TBPH^RNAi.UAS.cUa has lethal phenotype

(Appocher et al., 2017)

Scer\GAL4^elav.PU, Syp^GD9477, TBPH[+]/TBPH^Δ23, TBPH^RNAi.UAS.cUa has paralytic | adult stage phenotype

(Appocher et al., 2017)

Hrb27C^KK109074, Scer\GAL4^elav.PU, TBPH[+]/TBPH^Δ23, TBPH^RNAi.UAS.cUa has lethal phenotype

(Appocher et al., 2017)

Scer\GAL4^elav.PU, Syp^KK108062, TBPH[+]/TBPH^Δ23, TBPH^RNAi.UAS.cUa has paralytic | adult stage phenotype

(Appocher et al., 2017)

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

The reduced climbing ability of adult flies heterozygous for TBPH^Δ23 and also expressing TBPH^{dsRNA.Scer\UAS.cUa} (together with UAS-Dicer2 to enhance RNAi efficiency) driven by Scer\GAL4^elav.PU is strongly enhanced by co-expression of any of the following: CG42458^GD16040, CG42458^KK113285, sqd^GD8593, rump^GD14202, rump^KK102889, CG30122^KK100713 (which on their own do not affect adult locomotion), as well as by co-expression of: Hrb87F^KK108162, glo^GD12082, glo^KK108457, heph^GD10126, heph^KK108057, HnRNP-K^GD1382, HnRNP-K^KK106619, sm^GD12545 and sm^KK108588 (which own their own also induce climbing ability defects that are synergistically enhanced by combination with the TBPH hypomorphic test line).

Animals expressing either Hrb27C^GD6964 or Hrb27C^KK109074 (together with UAS-Dicer2 to enhance RNAi efficiency) under the control of Scer\GAL4^elav.PU in either wild-type or TBPH^Δ23/+; TBPH^{dsRNA.Scer\UAS.cUa} background are lethal.

Adult flies expressing either Syp^GD9477 or Syp^KK108062 (together with UAS-Dicer2 to enhance RNAi efficiency) under the control of Scer\GAL4^elav.PU in either wild-type or TBPH^Δ23/+; TBPH^{dsRNA.Scer\UAS.cUa} background appear to be completely paralyzed.

(Appocher et al., 2017)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (5)

Reported As

Symbol Synonym

TBPH^RNAi.UAS.cUa

TBPH^RNAi

(Lehmkuhl et al., 2021)

TBPH^{dsRNA.Scer\UAS.cUa}

TBPH^{dsRNA.UAS.cUa}

Name Synonyms

Saccharomyces cerevisiae UAS construct a of Unknown

Secondary FlyBase IDs

References (5)

Report Sections

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