UASt regulatory sequences drive expression of an inverted repeat.
Expression of HnRNP-KGD1382 under the control of Scer\GAL4GMR.PU has no obvious effect on adult eye morphology, whereas expression driven by Scer\GAL4elav.PU results in significant decrease in adult climbing ability.
Flies expressing blGD1382 under the control of Scer\GAL4Act88F.PB have significant defects in flight ability compared to wild type.
Adults expressing HnRNP-KGD1382 under the control of Scer\GAL4elav.PLu (in the presence of Dcr-2Scer\UAS.cDa to increase the efficiency of RNAi) do not show a significant defect in avoidance of noxious temperature (46[o]C) compared to control flies.
HnRNP-KGD1382, Scer\GAL4elav.PU has abnormal locomotor behavior | adult stage phenotype, enhanceable by TBPHRNAi.UAS.cUa/TBPH[+]/TBPHΔ23, Scer\GAL4elav.PU
HnRNP-KGD1382, Scer\GAL4GMR.PU is an enhancer of visible | adult stage phenotype of Scer\GAL4GMR.PU, TBPHUAS.Tag:FLAG
HnRNP-KGD1382, Scer\GAL4GMR.PU is an enhancer of melanotic mass phenotype | adult stage phenotype of Scer\GAL4GMR.PU, TBPHUAS.Tag:FLAG
HnRNP-KGD1382, Scer\GAL4elav.PU is an enhancer of abnormal locomotor behavior | adult stage phenotype of Scer\GAL4elav.PU, TBPHΔ23, TBPHRNAi.UAS.cUa
HnRNP-KGD1382, Scer\GAL4GMR.PU is an enhancer of eye phenotype of Scer\GAL4GMR.PU, TBPHUAS.Tag:FLAG
HnRNP-KGD1382, Scer\GAL4GMR.PU is an enhancer of ommatidium | adult stage phenotype of Scer\GAL4GMR.PU, TBPHUAS.Tag:FLAG
The climbing ability deficit observed both in the TBPH hypomorphic flies (TBPHΔ23/+;Scer\GAL4elav.PU>TBPHdsRNA.Scer\UAS.cUa, UAS-Dicer2) and flies expressing HnRNP-KGD1382 (also with UAS-Dicer2 and under Scer\GAL4elav.PU control) is much enhanced when the two mutants are combined.
The eye degeneration phenotype (appearance of melanotic spots, fusion of ommatidia) characteristic for adult flies expressing TBPHScer\UAS.T:Zzzz\FLAG under the control of Scer\GAL4GMR.PU is exacerbated further by co-expression of HnRNP-KGD1382.