FlyBase Allele Report: Dmel\Hrb27C[KK109074]

General Information

Symbol

Dmel\Hrb27C^KK109074

Species

D. melanogaster

Name

FlyBase ID

FBal0236028

Feature type

allele

Associated gene

Dmel\Hrb27C

Associated Insertion(s)

Carried in Construct

P{KK109074}

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Keleman et al., 2009.8.5)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Keleman et al., 2009.8.5)

Mutagen

in vitro construct

(Keleman et al., 2009.8.5)

Progenitor genotype

Carried in construct

P{KK109074}

Cytology

Description

UASt regulatory sequences drive expression of an inverted repeat.

(Keleman et al., 2009.8.5)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

dsRNA-KK109074

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

ameliorates neurodegenerative disease

modeled by TBPH^UAS.Tag:FLAG

(Appocher et al., 2017)

ameliorates amyotrophic lateral sclerosis type 10

modeled by TBPH^{UAS.Tag:FLAG.Tag:HA}

(Park et al., 2020)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neuroanatomy | adult stage, with Scer\GAL4^ey-OK107

(Bruckert et al., 2015)

abnormal size | third instar larval stage, with Scer\GAL4^hh-Gal4

(Mach et al., 2018)

decreased cell number | adult stage, with Scer\GAL4^ey-OK107

(Bruckert et al., 2015)

lethal, with Scer\GAL4^elav.PU

(Appocher et al., 2017)

Phenotype Manifest In

mushroom body | adult stage, with Scer\GAL4^ey-OK107

(Bruckert et al., 2015)

wing disc posterior compartment | third instar larval stage, with Scer\GAL4^hh-Gal4

(Mach et al., 2018)

Detailed Description

Statement

Reference

Expressing Hrb27C^KK109074 under the control of Scer\GAL4^hh-Gal4 causes a severe reduction of the wing disc posterior compartment in third instar larvae.

(Mach et al., 2018)

Expression of Hrb27C^KK109074 under the control of Scer\GAL4^GMR.PU has no obvious effect on adult eye morphology, whereas expression driven by Scer\GAL4^elav.PU is lethal.

(Appocher et al., 2017)

Expression of Hrb27C^KK109074 under the control of Scer\GAL4^ey-OK107 leads to a general decrease in Mushroom Body (MB) lobe volume associated with a decrease in the total number of MB neurons, MB αβ lobe defects (asymmetric, shorter or missing lobes) and abnormal overextension of αβ and α'β' dorsal lobes when compared to controls. Overextended dorsal branches systematically grow toward the midline, where, in extreme cases, meet with their counterparts. The proportion of MBs with axon overextension defects and the proportion of MBs with asymmetric or truncated α or β lobes are higher in females than in males.

(Bruckert et al., 2015)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressor of

Statement

Reference

Hrb27C^KK109074, Scer\GAL4^GMR.PU is a suppressor | partially of visible | adult stage phenotype of Scer\GAL4^GMR.PU, TBPH^UAS.Tag:FLAG

(Appocher et al., 2017)

Hrb27C^KK109074, Scer\GAL4^GMR.PU is a suppressor | partially of melanotic mass phenotype | adult stage phenotype of Scer\GAL4^GMR.PU, TBPH^UAS.Tag:FLAG

(Appocher et al., 2017)

Hrb27C^KK109074, Scer\GAL4^GMR.PU is a suppressor | partially of abnormal eye color phenotype of Scer\GAL4^GMR.PU, TBPH^UAS.Tag:FLAG

(Appocher et al., 2017)

Other

Statement

Reference

Hrb27C^KK109074, Scer\GAL4^elav.PU, TBPH[+]/TBPH^Δ23, TBPH^RNAi.UAS.cUa has lethal phenotype

(Appocher et al., 2017)

Phenotype Manifest In

Suppressor of

Statement

Reference

Hrb27C^KK109074, Scer\GAL4^GMR.PU is a suppressor | partially of eye phenotype of Scer\GAL4^GMR.PU, TBPH^UAS.Tag:FLAG

(Appocher et al., 2017)

Hrb27C^KK109074, Scer\GAL4^GMR.PU is a suppressor | partially of ommatidium | adult stage phenotype of Scer\GAL4^GMR.PU, TBPH^UAS.Tag:FLAG

(Appocher et al., 2017)

Additional Comments

Genetic Interactions

Statement

Reference

Animals expressing Hrb27C^KK109074 (together with UAS-Dicer2 to enhance RNAi efficiency) under the control of Scer\GAL4^elav.PU in either wild-type or TBPH^Δ23/+; TBPH^{dsRNA.Scer\UAS.cUa} background are lethal.

The eye degeneration phenotype (appearance of melanotic spots, fusion of ommatidia) characteristic for adult flies expressing TBPH^{Scer\UAS.T:Zzzz\FLAG} under the control of Scer\GAL4^GMR.PU is strongly suppressed by co-expression of Hrb27C^KK109074.

(Appocher et al., 2017)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Partially rescued by

Hrb27C^KK109074 is partially rescued by Scer\GAL4^ey-OK107/Hrb27C^UAS.Tag:FLAG

(Bruckert et al., 2015)

Comments

Expression of Hrb27C^UAS.Tag:FLAG under the control of Scer\GAL4^ey-OK107 partially rescues Mushroom Body axonal projection defects of Hrb27C^KK109074 expression under the control of Scer\GAL4^ey-OK107.

(Bruckert et al., 2015)

Images (0)

Mutant

Wild-type

Stocks (1)

VDRC

v101555

P{KK109074}VIE-260B

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

Hrb27C^KK109074

Hrb27C^RNAi

(Appocher et al., 2017)

Name Synonyms

Secondary FlyBase IDs

References (5)

Report Sections

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