TBPHΔ23/+ adult flies also expressing TBPHdsRNA.Scer\UAS.cUa (together with UAS-Dicer2 to enhance RNAi efficiency) under the control of Scer\GAL4elav.PU display significant locomotor deficit in climbing assays.
Motor neuron clones expressing TBPHdsRNA.Scer\UAS.cUa under the control of Scer\GAL4VGlut-OK371 do not exhibit any observable degeneration.
The retina of flies expressing TBPHdsRNA.Scer\UAS.cUa under the control of Scer\GAL4GMR.PU exhibit scattered vacuolar degeneration.
Scer\GAL4elav.PU, TBPHΔ23, TBPHRNAi.UAS.cUa has abnormal locomotor behavior | adult stage phenotype, enhanceable by CG42458GD16040, Scer\GAL4elav.PU
Scer\GAL4elav.PU, TBPHΔ23, TBPHRNAi.UAS.cUa has abnormal locomotor behavior | adult stage phenotype, enhanceable by CG42458KK113285, Scer\GAL4elav.PU
Scer\GAL4elav.PU, TBPHΔ23, TBPHRNAi.UAS.cUa has abnormal locomotor behavior | adult stage phenotype, enhanceable by Scer\GAL4elav.PU/hephKK108057
Scer\GAL4elav.PU, TBPHΔ23, TBPHRNAi.UAS.cUa has abnormal locomotor behavior | adult stage phenotype, enhanceable by HnRNP-KGD1382, Scer\GAL4elav.PU
Scer\GAL4elav.PU, TBPHΔ23, TBPHRNAi.UAS.cUa has abnormal locomotor behavior | adult stage phenotype, enhanceable by HnRNP-KKK106619, Scer\GAL4elav.PU
Scer\GAL4elav.PU, TBPHΔ23, TBPHRNAi.UAS.cUa has abnormal locomotor behavior | adult stage phenotype, enhanceable by Scer\GAL4elav.PU/smGD12545
Scer\GAL4elav.PU, TBPHΔ23, TBPHRNAi.UAS.cUa has abnormal locomotor behavior | adult stage phenotype, enhanceable by smKK108588/Scer\GAL4elav.PU
Scer\GAL4elav.PU, TBPHΔ23, TBPHRNAi.UAS.cUa has abnormal locomotor behavior | adult stage phenotype, enhanceable by sqdGD8593/Scer\GAL4elav.PU
Scer\GAL4elav.PU, TBPHΔ23, TBPHRNAi.UAS.cUa has abnormal locomotor behavior | adult stage phenotype, enhanceable by Scer\GAL4elav.PU/rumpGD14202
Scer\GAL4elav.PU, TBPHΔ23, TBPHRNAi.UAS.cUa has abnormal locomotor behavior | adult stage phenotype, enhanceable by Scer\GAL4elav.PU/rumpKK102889
Scer\GAL4elav.PU, TBPHΔ23, TBPHRNAi.UAS.cUa has abnormal locomotor behavior | adult stage phenotype, enhanceable by CG30122KK100713, Scer\GAL4elav.PU
Scer\GAL4elav.PU, TBPHΔ23, TBPHRNAi.UAS.cUa has abnormal locomotor behavior | adult stage phenotype, enhanceable by Hrb87FKK108162, Scer\GAL4elav.PU
Scer\GAL4elav.PU, TBPHΔ23, TBPHRNAi.UAS.cUa has abnormal locomotor behavior | adult stage phenotype, enhanceable by Scer\GAL4elav.PU/gloGD12082
Scer\GAL4elav.PU, TBPHΔ23, TBPHRNAi.UAS.cUa has abnormal locomotor behavior | adult stage phenotype, enhanceable by gloKK108457/Scer\GAL4elav.PU
Scer\GAL4elav.PU, TBPHΔ23, TBPHRNAi.UAS.cUa has abnormal locomotor behavior | adult stage phenotype, enhanceable by Scer\GAL4elav.PU/hephGD10126
TBPHRNAi.UAS.cUa, TBPH[+], TBPHΔ23, Scer\GAL4elav.PU is an enhancer of abnormal locomotor behavior | adult stage phenotype of HnRNP-KGD1382, Scer\GAL4elav.PU
TBPHRNAi.UAS.cUa, TBPH[+], TBPHΔ23, Scer\GAL4elav.PU is an enhancer of abnormal locomotor behavior | adult stage phenotype of HnRNP-KKK106619, Scer\GAL4elav.PU
TBPHRNAi.UAS.cUa, Scer\GAL4elav.PU, TBPH[+], TBPHΔ23 is an enhancer of abnormal locomotor behavior | adult stage phenotype of Scer\GAL4elav.PU, hephGD10126
TBPHRNAi.UAS.cUa, Scer\GAL4elav.PU, TBPH[+], TBPHΔ23 is an enhancer of abnormal locomotor behavior | adult stage phenotype of Scer\GAL4elav.PU, hephKK108057
TBPHRNAi.UAS.cUa, Scer\GAL4elav.PU, TBPH[+], TBPHΔ23 is an enhancer of abnormal locomotor behavior | adult stage phenotype of Scer\GAL4elav.PU, smGD12545
TBPHRNAi.UAS.cUa, Scer\GAL4elav.PU, TBPH[+], TBPHΔ23 is an enhancer of abnormal locomotor behavior | adult stage phenotype of Scer\GAL4elav.PU, smKK108588
TBPHRNAi.UAS.cUa, TBPH[+], TBPHΔ23, Scer\GAL4elav.PU is an enhancer of abnormal locomotor behavior | adult stage phenotype of Hrb87FKK108162, Scer\GAL4elav.PU
TBPHRNAi.UAS.cUa, Scer\GAL4elav.PU, TBPH[+], TBPHΔ23 is an enhancer of abnormal locomotor behavior | adult stage phenotype of Scer\GAL4elav.PU, gloGD12082
TBPHRNAi.UAS.cUa, Scer\GAL4elav.PU, TBPH[+], TBPHΔ23 is an enhancer of abnormal locomotor behavior | adult stage phenotype of Scer\GAL4elav.PU, gloKK108457
Hrb27CGD6964, Scer\GAL4elav.PU, TBPH[+]/TBPHΔ23, TBPHRNAi.UAS.cUa has lethal phenotype
Scer\GAL4elav.PU, SypGD9477, TBPH[+]/TBPHΔ23, TBPHRNAi.UAS.cUa has paralytic | adult stage phenotype
Hrb27CKK109074, Scer\GAL4elav.PU, TBPH[+]/TBPHΔ23, TBPHRNAi.UAS.cUa has lethal phenotype
Scer\GAL4elav.PU, SypKK108062, TBPH[+]/TBPHΔ23, TBPHRNAi.UAS.cUa has paralytic | adult stage phenotype
The reduced climbing ability of adult flies heterozygous for TBPHΔ23 and also expressing TBPHdsRNA.Scer\UAS.cUa (together with UAS-Dicer2 to enhance RNAi efficiency) driven by Scer\GAL4elav.PU is strongly enhanced by co-expression of any of the following: CG42458GD16040, CG42458KK113285, sqdGD8593, rumpGD14202, rumpKK102889, CG30122KK100713 (which on their own do not affect adult locomotion), as well as by co-expression of: Hrb87FKK108162, gloGD12082, gloKK108457, hephGD10126, hephKK108057, HnRNP-KGD1382, HnRNP-KKK106619, smGD12545 and smKK108588 (which own their own also induce climbing ability defects that are synergistically enhanced by combination with the TBPH hypomorphic test line).
Animals expressing either Hrb27CGD6964 or Hrb27CKK109074 (together with UAS-Dicer2 to enhance RNAi efficiency) under the control of Scer\GAL4elav.PU in either wild-type or TBPHΔ23/+; TBPHdsRNA.Scer\UAS.cUa background are lethal.
Adult flies expressing either SypGD9477 or SypKK108062 (together with UAS-Dicer2 to enhance RNAi efficiency) under the control of Scer\GAL4elav.PU in either wild-type or TBPHΔ23/+; TBPHdsRNA.Scer\UAS.cUa background appear to be completely paralyzed.