FlyBase Allele Report: Dmel\MED19[2]

General Information

Symbol

Dmel\MED19²

Species

D. melanogaster

Name

FlyBase ID

FBal0296246

Feature type

allele

Associated gene

Dmel\MED19

Associated Insertion(s)

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

amorphic allele - molecular evidence

Mutagen

P-element activity

Nature of the Allele

Allele class

amorphic allele - molecular evidence

(Boube et al., 2014)

Mutagen

P-element activity

(Boube et al., 2014)

Progenitor genotype

P{EPgy2}EY16159

(Boube et al., 2014)

Cytology

Description

Imprecise excision of the progenitor insertion, resulting in a 1174bp deletion that includes exon 1 of MED19.

(Boube et al., 2014)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

deletion

3L:17,845,369..17,846,542 [-]

Comment:

Inferred location of 1174 bp deletion resulting from the imprecise excision of P{EPgy2}EY16159, which extends from the insertion into MED19, removing the first exon.

(Boube et al., 2014)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

Detailed Description

Statement

Reference

Homozygotes die at the end of embryogenesis, without cuticular defects. Embryos derived from homozygous female germline clones undertake development, but abnormalities are visible by the pre-cellular blastoderm, resulting in extremely disorganised cellular embryos that die soon after.

Homozygous somatic clones are not recovered in the imaginal discs, indicating cell lethality, but they do survive if they are induced in a Minute background. Homozygous clones induced in a Minute background in the dorsal compartment of the haltere disc result in disorganisation and a reduction in number of the pedicellar sensillae.

MED19¹/MED19² pupae show a fully penetrant loss of anterior spiracles.

MED19² homozygotes carrying MED19^Ubi.PB occasionally survive to adulthood. These escapers show loss of maxillary palps.

(Boube et al., 2014)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressor of

Statement

Reference

MED19²/MED19[+] is a suppressor of visible | dominant phenotype of Antp^Ns-rvC3

(Boube et al., 2014)

MED19²/MED19[+] is a suppressor of homeotic | dominant phenotype of Antp^Ns-rvC3

(Boube et al., 2014)

MED19²/MED19[+] is a suppressor of visible | dominant phenotype of Dfd¹

(Boube et al., 2014)

MED19²/P{EPgy2}EY16159 is a suppressor of visible phenotype of Ubx^Cbx-1

(Boube et al., 2014)

MED19²/P{EPgy2}EY16159 is a suppressor of homeotic phenotype of Ubx^Cbx-1

(Boube et al., 2014)

Phenotype Manifest In

Suppressor of

Statement

Reference

MED19²/MED19[+] is a suppressor of antenna phenotype of Antp^Ns-rvC3

(Boube et al., 2014)

MED19²/MED19[+] is a suppressor of leg | ectopic phenotype of Antp^Ns-rvC3

(Boube et al., 2014)

MED19²/MED19[+] is a suppressor of maxillary palp | ectopic phenotype of Dfd¹

(Boube et al., 2014)

MED19²/P{EPgy2}EY16159 is a suppressor of wing blade posterior compartment phenotype of Ubx^1:Cbx-1

(Boube et al., 2014)

MED19²/P{EPgy2}EY16159 is a suppressor of haltere | ectopic phenotype of Ubx^Cbx-1

(Boube et al., 2014)

Additional Comments

Genetic Interactions

Statement

Reference

MED19²/+ partially suppresses the antenna-to-leg transformation seen in Antp^Ns-rvC3/+ flies, such that the double heterozygotes have antennal aristae.

The ectopic maxillary palp phenotype seen in Dfd¹/+ flies is fully suppressed by MED19²/+.

The transformation of posterior wing to hemi-haltere seen in Ubx^Cbx-1 homozygotes is no longer seen if the flies are also carrying MED19²/P{EPgy2}EY16159 and the cellular trichomes are reoriented along the long wing axis.

(Boube et al., 2014)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

MED19²/MED19¹ is rescued by Scer\GAL4^arm.PU/MED19^UAS.C-Venus

(Boube et al., 2014)

MED19²/MED19¹ is rescued by MED19^Ubi.PB

(Boube et al., 2014)

MED19² is rescued by Scer\GAL4^en.PU/MED19^UAS.C-Venus

(Boube et al., 2014)

MED19² is rescued by Scer\GAL4^en.PU/MED19^{ΔHIM.UAS.C-Venus}

(Boube et al., 2014)

Partially rescued by

MED19² is partially rescued by MED19^Ubi.PB

(Boube et al., 2014)

Not rescued by

MED19²/MED19¹ is not rescued by MED19^{ΔHIM.UAS.C-Venus}/Scer\GAL4^arm.PU

(Boube et al., 2014)

Comments

Expression of MED19^{Scer\UAS.T:Avic\GFP-VC} under the control of Scer\GAL4^arm.PU rescues the lethality of MED19¹/MED19² animals.

Expression of MED19^{Scer\UAS.T:Avic\GFP-VC} under the control of Scer\GAL4^en.PU rescues the cell lethality of homozygous MED19² somatic clones.

Expression of MED19^{ΔHIM.Scer\UAS.T:Avic\GFP-VC} under the control of Scer\GAL4^arm.PU fails to rescue the lethality of MED19¹/MED19² animals.

Expression of MED19^{ΔHIM.Scer\UAS.T:Avic\GFP-VC} under the control of Scer\GAL4^en.PU rescues the cell lethality of homozygous MED19² somatic clones.

(Boube et al., 2014)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

MED19²

Med19²

(Immarigeon et al., 2020, Boube et al., 2014)

Name Synonyms

Secondary FlyBase IDs

References (2)

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