MED19²/+ partially suppresses the antenna-to-leg transformation seen in Antp^Ns-rvC3/+ flies, such that the double heterozygotes have antennal aristae.

The number of LinA-derived neurons is dramatically reduced compared to wild type in Scr^C1 Antp^Ns-rvC3 Ubx^MX12 triple mutant clones examined at the late third instar larval stage. This phenotype is seen in all three thoracic segments. The number of glia derived from the triple mutant LinA neuroblast clones is also reduced compared to wild type.

Adult motor neurons derived from Scr^C1 Antp^Ns-rvC3 Ubx^MX12 triple mutant LinA neuroblast clones target the same region of the leg as do wild-type LinA-derived neurons. However, fewer branches are observed compared with wild-type clones, with the distal region of the tibia being most highly affected.

Single cell class IV dendrite arborisation (da) neuron clones that are triply mutant for Scr^C1, Antp^Ns-rvC3, Ubx^MX12 show severe defects in dendrite growth, branching and coverage in segment T3 as well as axon fasciculation defects. Analysis of Scr^C1, Antp^Ns-rvC3, Ubx^MX12 triple mutant ddaC neuron clones indicates that although the major dendrites of the mutant neurons grow to a similar extent as wild-type controls in the interval between 80 and 96 hours after egg laying, the mutant clones have an increased number of terminal dendrites compared to controls. The mutant clones also show increased dynamic behaviour over this time period compared to controls, due to increased dendrite branch initiation/growth. The number of dendrite retractions is not altered in the mutant neurons.

Scr^C1 Antp^Ns-rvC3 double mutant embryos develop ectopic sclerites, which often look like the cuticular scar tissue that often surrounds the healed wound generated by a sterile needle in late-stage wild-type embryos.

Scr^C1 Antp^Ns-rvC3 double mutant stage 17 embryos have failures in epidermal integrity; dye injected into the perivitelline space penetrates the body cavity in the mutant embryos.

Antp^Ns-rvC3 ss^D115.7 double mutant clones in the leg shoe segment fusions, but no transformation of leg to antenna.

Appendages in thoracic segment 2 (T2) which are composed of a Antp^Ns-rvC3 hth^Meis1-P2 double mutant clone are leg-like along their entire proximo-distal (P-D) axis. However they have only two distinct segments along the P-D axis; a complete tarsus (with five subsegments and a claw) and a single proximal segment (which likely results from a fusion of the four proximal-most segments of a wild-type leg). The appendage shows polarity along the P-D axis (as in wild type), with bristles and trichomes usually pointing distally and distinct bristle types being seen at different positions along the P-D axis. In the proximal segment, two to three spurs are usually seen distally (this type of bristle is normally found in the distal tibia). Antp^Ns-rvC3 hth^Meis1-P2 Scr^C1 triple mutant clones result in two-segment appendages in T1 and T2.

The transformation of genitalia into leg seen in Abd-B^D18 mutant clones is still seen in Abd-B^D18 Antp^Ns-rvC3 double mutants.

Antp^Ns-rvC3 Scr^C1 abd-A^M1 Abd-B^M8 quadruple mutant embryos secrete cuticle which has a reiteration of the A1 segment throughout the abdomen. If these embryos are also mutant for hth^C1 the abdominal segments all resemble the third abdominal segment.

Quintuple Scr^C1, Antp^Ns-rvC3, Ubx^MX2, abd-A^M1, Abd-B^M8 homozygous larvae (deficient for activity of thoracic and abdominal homeotic genes) exhibit sclerotic plates (sp) anterior to each denticle belt. Expression of Dfd^hs.PK, lab^hs.PH or Ubx^hs.PG suppresses the differentiation of the sp.