FlyBase Allele Report: Dmel\RhoGAP18B[1]

General Information

Symbol

Dmel\RhoGAP18B¹

Species

D. melanogaster

Name

FlyBase ID

FBal0240542

Feature type

allele

Associated gene

Dmel\RhoGAP18B

Associated Insertion(s)

P{GawB}RhoGAP18B¹

Carried in Construct

Also Known As

whir¹, whir¹-Gal4

Key Links

Allele class

Mutagen

P-element activity

Nature of the Allele

Allele class

Mutagen

P-element activity

(Rothenfluh et al., 2006)

Progenitor genotype

Associated Insertion(s)

P{GawB}RhoGAP18B¹

Cytology

Description

A P-element is inserted 5' of the RD transcription start site, in the ORF of the RC transcript.

(Rothenfluh et al., 2006)

Allele components

Component

Use(s)

Inserted element

P{GawB}

enhancer trap

Encoded product / tool

GAL4

binary expression system - driver

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Disease

Evidence

References

model of alcohol use disorder

CEA

(Gonzalez et al., 2018, Ojelade et al., 2015, Rothenfluh et al., 2006)

CEA with tsr^k05633

(Ojelade et al., 2015)

CEA with tsr^N121

(Ojelade et al., 2015)

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

model of alcohol use disorder

is ameliorated by LIMK1^EY08757

(Ojelade et al., 2015)

is ameliorated by Efa6^c00953

(Gonzalez et al., 2018)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal smell perception

(Joseph et al., 2009)

chemical resistant

(Peru Y Colón de Portugal et al., 2012, Rothenfluh et al., 2006)

chemical resistant | adult stage

(Ojelade et al., 2015)

chemical resistant | adult stage | recessive

(Gonzalez et al., 2018)

viable

(Rothenfluh et al., 2006)

Phenotype Manifest In

Detailed Description

Statement

Reference

RhoGAP18B¹ homozygotes are more resistant (higher survival) to ethanol than heterozygotes or wild-type controls.

(Gonzalez et al., 2018)

RhoGAP18B¹/RhoGAP18B¹ mutant adults show increased resistance to ethanol-induced sedation (measured by a loss of righting assay) compared to wild-type.

(Ojelade et al., 2015)

Homozygous flies show resistance to ethanol-induced sedation.

(Peru Y Colón de Portugal et al., 2012)

Positional aversion to acetic acid is increased in RhoGAP18B¹ females, an effect that is significantly diminished by antennal removal. This increased positional repulsion is accompanied by egg-laying aversion for acetic acid containing food; this effect is strongly ameliorated by antennaectomy. Similarly, removing the antenna of RhoGAP18B¹ males reduces their excessive positional repulsion to acetic acid.

(Joseph et al., 2009)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

RhoGAP18B¹ has chemical resistant | recessive | adult stage phenotype, suppressible by Efa6[+]/Efa6^c00953

(Gonzalez et al., 2018)

RhoGAP18B¹ has chemical resistant | adult stage phenotype, suppressible by LIMK1^EY08757/LIMK1^EY08757

(Ojelade et al., 2015)

RhoGAP18B¹ has chemical resistant phenotype, suppressible | partially by Arfip¹²/Arfip[+]

(Peru Y Colón de Portugal et al., 2012)

RhoGAP18B¹ has chemical resistant phenotype, suppressible | partially by Arf51F[+]/Arf6^NP5226

(Peru Y Colón de Portugal et al., 2012)

RhoGAP18B¹ has chemical resistant phenotype, suppressible by Rho1[+]/Rho1^k02107b

(Rothenfluh et al., 2006)

RhoGAP18B¹ has chemical resistant phenotype, suppressible by Rac2^Δ/Mtl[+]/Mtl^Δ/Rac2[+]/Rac1^J10/Rac1[+]

(Rothenfluh et al., 2006)

NOT Enhancer of

Statement

Reference

RhoGAP18B¹ is a non-enhancer of chemical sensitive phenotype of Arf6^NP5226/Arf6^EP2612

(Peru Y Colón de Portugal et al., 2012)

NOT Suppressor of

Statement

Reference

RhoGAP18B¹ is a non-suppressor of chemical sensitive phenotype of Arf6^NP5226/Arf6^EP2612

(Peru Y Colón de Portugal et al., 2012)

Other

Statement

Reference

RhoGAP18B¹, tsr^N121/tsr[+] has chemical resistant | adult stage phenotype

(Ojelade et al., 2015)

RhoGAP18B¹, tsr[+]/tsr^k05633 has chemical resistant | adult stage phenotype

(Ojelade et al., 2015)

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

The increased resistance to ethanol-induced sedation observed in RhoGAP18B¹/RhoGAP18B¹ adults can be suppressed by combination with LIMK1^EY08757/LIMK1^EY08757.

The increased resistance to ethanol-induced sedation observed in RhoGAP18B¹/RhoGAP18B¹ adults cannot be suppressed by combination with tsr^N121 in heterozygous state but RhoGAP18B¹/+;tsr^N121/+ double heterozygotes display significantly higher resistance than RhoGAP18B¹/+ single heterozygotes.

The increased resistance to ethanol-induced sedation observed in RhoGAP18B¹/RhoGAP18B¹ adults cannot be suppressed by combination with tsr^k05633 in heterozygous state but RhoGAP18B¹/+;tsr^k05633/+ double heterozygotes display significantly higher resistance than RhoGAP18B¹/+ single heterozygotes.

(Ojelade et al., 2015)

Arfip¹²/+ and Arf51F^NP5226/+ each partially suppress the resistance to ethanol-induced sedation seen in RhoGAP18B¹ homozygotes.

RhoGAP18B¹/+ ; Arfip¹²/+ and RhoGAP18B¹/+ ; Arf51F^NP5226/+ double heterozygotes show wild-type sensitivity to ethanol in a sedation assay.

The sensitivity to ethanol-induced sedation seen in Arf51F^KG02753/Arf51F^EP2612 flies is not altered if they also carry RhoGAP18B¹.

(Peru Y Colón de Portugal et al., 2012)

Heterozygosity for Rho1^k02107b or Df(2R)Jp6 (which removes Rho1) strongly suppresses the RhoGAP18B¹ ethanol-resistance phenotype.

Heterozygosity for Rac1^J10 Rac2^Δ Mtl^Δ suppresses the RhoGAP18B¹ ethanol-resistance phenotype.

(Rothenfluh et al., 2006)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Fails to complement

RhoGAP18B^ΔRC

(Rothenfluh et al., 2006)

Rescued by

RhoGAP18B¹ is rescued by RhoGAP18B^RC.UAS/Scer\GAL4^RhoGAP18B-1

(Rothenfluh et al., 2006)

Comments

Expression of RhoGAP18B^RC.Scer\UAS under the control of Scer\GAL4^whir1 in RhoGAP18B¹ mutants ameliorates the ethanol-resistance phenotype of RhoGAP18B¹. The phenotype rescue is, however, incomplete, probably due to the fact that expression of RhoGAP18B^RC.Scer\UAS under the control of Scer\GAL4^whir1 is low.

(Rothenfluh et al., 2006)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

Comments

Based on resistance to enthanol-induced sedation, the following RhoGAP18B alleles can be ranked from strongest to weakest as follows: RhoGAP18B³ > RhoGAP18B¹ = RhoGAP18B^ΔRC.

(Rothenfluh et al., 2006)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

RhoGAP18B¹

whir¹

(Gonzalez et al., 2018, Ojelade et al., 2015, Peru Y Colón de Portugal et al., 2012, Joseph et al., 2009, Rothenfluh et al., 2006)

Name Synonyms

Secondary FlyBase IDs

References (5)

Report Sections

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