FlyBase Allele Report: Dmel\tsr[N121]

General Information

Symbol

Dmel\tsr^N121

Species

D. melanogaster

Name

FlyBase ID

FBal0177372

Feature type

allele

Associated gene

Dmel\tsr

Associated Insertion(s)

Carried in Construct

Key Links

Allele class

loss of function allele

Mutagen

P-element activity

Nature of the Allele

Allele class

loss of function allele

(Johnson et al., 2008, Ng and Luo, 2004)

Mutagen

P-element activity

(Ng and Luo, 2004)

Progenitor genotype

Cytology

Description

P-element-mediated imprecise excision of the tsr locus.

(Ng and Luo, 2004)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Disease

Evidence

References

model of alcohol use disorder

CEA with RhoGAP18B¹

(Ojelade et al., 2015)

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

ameliorates transthyretin amyloidosis

modeled by Hsap\TTR^{V30M.UAS.Tag:HA}

(Oliveira da Silva et al., 2020)

exacerbates Huntington's disease

modeled by Hsap\HTT^{128Q.1-231.UAS}

(Onur et al., 2021)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neuroanatomy | larval stage | somatic clone

(Stürner et al., 2022)

abnormal neuroanatomy | somatic clone

(Ng and Luo, 2004)

visible | somatic clone

(Ren et al., 2007)

Phenotype Manifest In

adult mushroom body & axon | somatic clone

(Ng and Luo, 2004)

adult mushroom body | somatic clone

(Ng and Luo, 2004)

dendrite | larval stage

(Stürner et al., 2022)

dendritic branch | decreased number | larval stage | somatic clone

(Stürner et al., 2022)

filamentous actin | somatic clone

(Ren et al., 2007)

larval multidendritic class III neuron | somatic clone

(Stürner et al., 2022)

tracheal lumen | somatic clone

(Schottenfeld-Roames et al., 2014)

wing hair | somatic clone

(Ren et al., 2007)

Detailed Description

Statement

Reference

tsr^N121 mutant cells display small cystic dilations along their seamless tracheal tubes.

(Schottenfeld-Roames et al., 2014)

When homozygous clones are induced from 4-5 days after egg laying, small clones that display a strong flr-like hair phenotype are seen in the adult wing. These clones are rarely larger than 8 cells. Few mutant clones are recovered in the adult wing if they are induced 3-4 days AEL.

Homozygous clones in the pupal wing show a dramatic increase in the level of F-actin compared to wild type, and this increase is seen both prior to and during wing hair morphogenesis.

(Ren et al., 2007)

Homozygous mushroom body neuroblast clones that are generated in newly hatched larvae contain only 15-30 neurons in the adult, in contrast to wild-type control clones, which contain approximately 400 neurons. Single cell homozygous clones of γ neurons in the mushroom body show axon growth defects in the adult; 30-40% of axons do not enter the medial lobe, stopping before the α/β branch point, 35-40% fail to extend beyond the midpoint of the γ lobe and 15-20% fail to reach the end of the γ lobe. Homozygous neuroblast clones that contain only α/β neurons fail to extend fully to the midline or to the dorsal cortex.

(Ng and Luo, 2004)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhancer of

Statement

Reference

tsr^N121/tsr[+] is an enhancer of abnormal planar polarity phenotype of Scer\GAL4^GMR.PF, cindr^{RNAi.PC.PD.UAS}

(Johnson et al., 2008)

Suppressor of

Statement

Reference

tsr^N121 is a suppressor of visible | adult stage phenotype of Hsap\TTR^{V30M.UAS.Tag:HA}, Scer\GAL4^GMR.PU

(Oliveira da Silva et al., 2020)

Other

Statement

Reference

RhoGAP18B¹, tsr^N121/tsr[+] has chemical resistant | adult stage phenotype

(Ojelade et al., 2015)

Phenotype Manifest In

Enhancer of

Statement

Reference

tsr^N121/tsr[+] is an enhancer of ommatidium phenotype of Scer\GAL4^GMR.PF, cindr^{RNAi.PC.PD.UAS}

(Johnson et al., 2008)

tsr^N121/tsr[+] is an enhancer of pigment cell phenotype of Scer\GAL4^GMR.PF, cindr^{RNAi.PC.PD.UAS}

(Johnson et al., 2008)

tsr^N121/tsr[+] is an enhancer of cone cell phenotype of Scer\GAL4^GMR.PF, cindr^{RNAi.PC.PD.UAS}

(Johnson et al., 2008)

Suppressor of

Statement

Reference

tsr^N121 is a suppressor of eye phenotype of Hsap\TTR^{V30M.UAS.Tag:HA}, Scer\GAL4^GMR.PU

(Oliveira da Silva et al., 2020)

Additional Comments

Genetic Interactions

Statement

Reference

The increased resistance to ethanol-induced sedation observed in RhoGAP18B¹/RhoGAP18B¹ adults cannot be suppressed by combination with tsr^N121 in heterozygous state but RhoGAP18B¹/+;tsr^N121/+ double heterozygotes display significantly higher resistance than RhoGAP18B¹/+ single heterozygotes.

(Ojelade et al., 2015)

A tsr^N121 heterozygous background enhances the patterning defects found in Scer\GAL4^GMR.PF>cindr^{dsRNA.PC.PD.Scer\UAS} mutants. The mean interommatidial precursor cell number and the number of cone and/or 1[o] cell errors is increased in these double mutants.

(Johnson et al., 2008)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

9109

w^*; P{FRT(w^hs)}G13 tsr^N121/CyO

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

tsr^N121

(Stürner et al., 2022, Onur et al., 2021, Oliveira da Silva et al., 2020, Sudarsanam et al., 2020, Wolterhoff et al., 2020, Nithianandam and Chien, 2018, Ojelade et al., 2015, Ren et al., 2007)

tsr^N121A

(Johnson et al., 2008)

twinstar N121

(Stürner et al., 2022)

Name Synonyms

Secondary FlyBase IDs

References (11)

Report Sections

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