FlyBase Allele Report: Dmel\Arf6[NP5226]

General Information

Symbol

Dmel\Arf6^NP5226

Species

D. melanogaster

Name

FlyBase ID

FBal0222345

Feature type

allele

Associated gene

Dmel\Arf6

Associated Insertion(s)

P{GawB}Arf6^NP5226

Carried in Construct

Also Known As

Arf6^Gal4, Arf6^G4, NP5226

Key Links

Genomic Maps

JBrowse

Allele class

amorphic allele - genetic evidence

Mutagen

Nature of the Allele

Allele class

amorphic allele - genetic evidence

(Peru Y Colón de Portugal et al., 2012)

Mutagen

Progenitor genotype

Associated Insertion(s)

P{GawB}Arf6^NP5226

Cytology

Description

Allele components

Component

Use(s)

Inserted element

P{GawB}

enhancer trap

Encoded product / tool

GAL4

binary expression system - driver

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

transposable_element_insertion_site

2R:15,322,854..15,322,854 [-]

(Gonzalez et al., 2018, Peru Y Colón de Portugal et al., 2012, FlyBase Curators, 2008-)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Disease

Evidence

References

model of alcohol use disorder

CEA with Arf6^EP2612

(Gonzalez et al., 2018)

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

chemical sensitive (with Arf6^EP2612)

(Peru Y Colón de Portugal et al., 2012)

chemical sensitive | adult stage (with Arf6^EP2612)

(Gonzalez et al., 2018)

Phenotype Manifest In

Detailed Description

Statement

Reference

Arf51F^EP2612/Arf51F^NP5226 transheterozygotes are more sensitive (lower survival) to ethanol than heterozygotes or wild-type controls; Arf51F^EP2612/Arf51F^NP5226 transheterozygotes do not develop much tolerance from previous exposures.

Naive Arf51F^EP2612/Arf51F^NP5226 mutants show preference for ethanol-laced food, unlike the aversion normally observed in wild-type controls. Arf51F^EP2612/Arf51F^NP5226 mutants previously exposed to ethanol show the same preference for ethanol-laced food as wild-type controls.

(Gonzalez et al., 2018)

Arf51F^NP5226/Arf51F^EP2612 flies are sensitive to ethanol-induced sedation compared to wild-type controls.

Arf51F^NP5226/Arf51F^EP2612 flies show a small but significant increase in sensitivity to sedation with CO[[2]] compared to wild type.

(Peru Y Colón de Portugal et al., 2012)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Arf6^NP5226/Arf6^EP2612 has chemical sensitive phenotype, enhanceable by amn^chpd

(Peru Y Colón de Portugal et al., 2012)

NOT Enhanced by

Statement

Reference

Arf6^NP5226/Arf6^EP2612 has chemical sensitive phenotype, non-enhanceable by Arfip¹²/Arfip¹²

(Peru Y Colón de Portugal et al., 2012)

Arf6^NP5226/Arf6^EP2612 has chemical sensitive phenotype, non-enhanceable by RhoGAP18B¹

(Peru Y Colón de Portugal et al., 2012)

Arf6^NP5226/Arf6^EP2612 has chemical sensitive phenotype, non-enhanceable by Scer\GAL4^NP1514/Rac1^CA.UAS

(Peru Y Colón de Portugal et al., 2012)

NOT suppressed by

Statement

Reference

Arf6^NP5226/Arf6^EP2612 has chemical sensitive phenotype, non-suppressible by RhoGAP18B¹

(Peru Y Colón de Portugal et al., 2012)

Arf6^NP5226/Arf6^EP2612 has chemical sensitive phenotype, non-suppressible by Scer\GAL4^NP1514/Rac1^CA.UAS

(Peru Y Colón de Portugal et al., 2012)

Suppressor of

Statement

Reference

Arf51F[+]/Arf6^NP5226 is a suppressor of partially lethal - majority die phenotype of RhoGAP18B^NP1514

(Gonzalez et al., 2018)

Arf51F[+]/Arf6^NP5226 is a suppressor | partially of chemical resistant phenotype of RhoGAP18B¹

(Peru Y Colón de Portugal et al., 2012)

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Arf51F^NP5226/+ partially suppresses the resistance to ethanol-induced sedation seen in RhoGAP18B¹ homozygotes.

RhoGAP18B¹/+ ; Arf51F^NP5226/+ double heterozygotes show wild-type sensitivity to ethanol in a sedation assay.

The sensitivity to ethanol-induced sedation seen in Arf51F^NP5226/Arf51F^EP2612 flies is not enhanced if they are also homozygous for Arfip¹².

The sensitivity to ethanol-induced sedation seen in Arf51F^NP5226/Arf51F^EP2612 flies is enhanced if they also carry amn^chpd.

(Peru Y Colón de Portugal et al., 2012)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

Arf6^NP5226/Arf6^EP2612 is rescued by Scer\GAL4^NP5226/Arf6^UAS.cCa

(Gonzalez et al., 2018)

Arf6^NP5226/Arf6^EP2612 is rescued by Scer\GAL4^NP5226/Arf6^UAS.cCa

(Peru Y Colón de Portugal et al., 2012)

Comments

Expression of Arf51F^Scer\UAS.cCa under the control of Scer\GAL4^{Arf51F-NP5226} rescues the sensitivity of Arf51F^NP5226/Arf51F^EP2612 flies to ethanol-induced sedation. This rescue is suppressed in the presence of Scer\GAL80^elav.PY.

(Peru Y Colón de Portugal et al., 2012)

Images (0)

Mutant

Wild-type

Stocks (1)

Kyoto

104910

y^* w^*; P{GawB}Arf6^NP5226 / CyO, P{UAS-lacZ.UW14}UW14

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

Arf51F^NP5226

Arf6^G4

(Gonzalez et al., 2018)

Arf6^Gal4

(Gonzalez et al., 2018, Peru Y Colón de Portugal et al., 2012)

Arf6^NP5226

Name Synonyms

Secondary FlyBase IDs

References (3)

Report Sections

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