FlyBase Allele Report: Dmel\Arf6[EP2612]

General Information

Symbol

Dmel\Arf6^EP2612

Species

D. melanogaster

Name

FlyBase ID

FBal0131798

Feature type

allele

Associated gene

Dmel\Arf6

Associated Insertion(s)

P{EP}Arf6^EP2612

Carried in Construct

Also Known As

Arf6^P2, EP2612, EP(2)2612

Key Links

Genomic Maps

JBrowse

Allele class

amorphic allele - genetic evidence

Mutagen

P-element activity

Nature of the Allele

Allele class

amorphic allele - genetic evidence

(Peru Y Colón de Portugal et al., 2012)

Mutagen

P-element activity

(Pena-Rangel et al., 2002, Gene Disruption Project members, 2001-)

Progenitor genotype

Associated Insertion(s)

P{EP}Arf6^EP2612

Cytology

Description

Insertion in the first intron.

(Dyer et al., 2007)

Allele components

Component

Use(s)

Inserted element

P{EP}

misexpression element

Regulatory region(s)

UAS

binary expression system - regulatory region

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

transposable_element_insertion_site

2R:15,322,778..15,322,778 [-]

(Acevedo et al., 2015, Kavi et al., 2015, Johnson et al., 2011, Dyer et al., 2007, Pena-Rangel et al., 2002, Gene Disruption Project members, 2001-)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Disease

Evidence

References

model of alcohol use disorder

CEA

(Gonzalez et al., 2018)

CEA with Arf6^NP5226

(Gonzalez et al., 2018)

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

Detailed Description

Statement

Reference

Arf51F^EP2612 homozygotes and Arf51F^EP2612/Arf51F^NP5226 transheterozygotes are more sensitive (lower survival) to ethanol than heterozygotes or wild-type controls; Arf51F^EP2612/Arf51F^NP5226 transheterozygotes do not develop much tolerance from previous exposures.

Naive Arf51F^EP2612/Arf51F^NP5226 mutants show preference for ethanol-laced food, unlike the aversion normally observed in wild-type controls. Arf51F^EP2612/Arf51F^NP5226 mutants previously exposed to ethanol show the same preference for ethanol-laced food as wild-type controls.

(Gonzalez et al., 2018)

Homozygotes are sensitive to ethanol-induced sedation compared to heterozygous siblings and wild-type controls.

Arf51F^NP5226/Arf51F^EP2612 and Arf51F^KG02753/Arf51F^EP2612 flies are sensitive to ethanol-induced sedation compared to wild-type controls.

Arf51F^NP5226/Arf51F^EP2612 flies show a small but significant increase in sensitivity to sedation with CO[[2]] compared to wild type.

(Peru Y Colón de Portugal et al., 2012)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Arf6^NP5226/Arf6^EP2612 has chemical sensitive phenotype, enhanceable by amn^chpd

(Peru Y Colón de Portugal et al., 2012)

NOT Enhanced by

Statement

Reference

Arf6^EP2612 has chemical sensitive | adult stage | recessive phenotype, non-enhanceable by Efa6^c00953/Efa6^c00953

(Gonzalez et al., 2018)

Arf6^NP5226/Arf6^EP2612 has chemical sensitive phenotype, non-enhanceable by Arfip¹²/Arfip¹²

(Peru Y Colón de Portugal et al., 2012)

Arf6^NP5226/Arf6^EP2612 has chemical sensitive phenotype, non-enhanceable by RhoGAP18B¹

(Peru Y Colón de Portugal et al., 2012)

Arf6^NP5226/Arf6^EP2612 has chemical sensitive phenotype, non-enhanceable by Scer\GAL4^NP1514/Rac1^CA.UAS

(Peru Y Colón de Portugal et al., 2012)

NOT suppressed by

Statement

Reference

Arf6^NP5226/Arf6^EP2612 has chemical sensitive phenotype, non-suppressible by RhoGAP18B¹

(Peru Y Colón de Portugal et al., 2012)

Arf6^NP5226/Arf6^EP2612 has chemical sensitive phenotype, non-suppressible by Scer\GAL4^NP1514/Rac1^CA.UAS

(Peru Y Colón de Portugal et al., 2012)

NOT Enhancer of

Statement

Reference

Arf6^EP2612/Arf6^EP2612 is a non-enhancer of chemical sensitive | adult stage | recessive phenotype of Efa6^c00953

(Gonzalez et al., 2018)

Other

Statement

Reference

Arf6^EP2612, Scer\GAL4^pnr-MD237, pnr^MD237 has lethal | embryonic stage phenotype

(Pena-Rangel et al., 2002)

Arf6^EP2612, Scer\GAL4^pnr-MD237, pnr^MD237/pnr[+] has lethal | embryonic stage phenotype

(Pena-Rangel et al., 2002)

Phenotype Manifest In

Enhancer of

Statement

Reference

Arf51F[+]/Arf6^EP2612 is an enhancer of ommatidium phenotype of Scer\GAL4^GMR.PF, cindr^{RNAi.PC.PD.UAS}

(Johnson et al., 2011)

Additional Comments

Genetic Interactions

Statement

Reference

The sensitivity to ethanol-induced sedation seen in Arf51F^NP5226/Arf51F^EP2612 flies is not enhanced if they are also homozygous for Arfip¹².

The sensitivity to ethanol-induced sedation seen in Arf51F^NP5226/Arf51F^EP2612 flies is enhanced if they also carry amn^chpd.

The sensitivity to ethanol-induced sedation seen in Arf51F^KG02753/Arf51F^EP2612 flies is not altered if they also carry RhoGAP18B¹.

The sensitivity to ethanol-induced sedation seen in Arf51F^KG02753/Arf51F^EP2612 flies is not altered if they express Rac1^CA.Scer\UAS under the control of Scer\GAL4^{RhoGAP18B-NP1514} specifically in the adult (using the heat sensitive Scer\GAL80^ts.αTub84B allele to limit expression).

(Peru Y Colón de Portugal et al., 2012)

The ommatidial patterning errors resulting from the expression of cindr^{dsRNA.PC.PD.Scer\UAS} via Scer\GAL4^GMR.PF are strongly enhanced in Arf51F^EP2612 heterozygotes.

(Johnson et al., 2011)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

Arf6^NP5226/Arf6^EP2612 is rescued by Scer\GAL4^NP5226/Arf6^UAS.cCa

(Gonzalez et al., 2018)

Arf6^NP5226/Arf6^EP2612 is rescued by Scer\GAL4^NP5226/Arf6^UAS.cCa

(Peru Y Colón de Portugal et al., 2012)

Arf6^EP2612/Arf6^KG02753 is rescued by Arf6^UAS.cCa/Scer\GAL4^elav.PLu

(Peru Y Colón de Portugal et al., 2012)

Arf6^EP2612/Arf6^KG02753 is rescued by Scer\GAL4^NP1514/Arf6^UAS.cCa

(Peru Y Colón de Portugal et al., 2012)

Comments

Expression of Arf51F^Scer\UAS.cCa under the control of Scer\GAL4^{Arf51F-NP5226} rescues the sensitivity of Arf51F^NP5226/Arf51F^EP2612 flies to ethanol-induced sedation. This rescue is suppressed in the presence of Scer\GAL80^elav.PY.

Expression of Arf51F^Scer\UAS.cCa under the control of Scer\GAL4^elav.PLu rescues the sensitivity of Arf51F^KG02753/Arf51F^EP2612 flies to ethanol-induced sedation.

Expression of Arf51F^Scer\UAS.cCa under the control of Scer\GAL4^{RhoGAP18B-NP1514} throughout development but not in the adult (using the heat sensitive Scer\GAL80^ts.αTub84B allele to limit expression) does not rescue the sensitivity of Arf51F^KG02753/Arf51F^EP2612 flies to ethanol-induced sedation.

Expression of Arf51F^Scer\UAS.cCa under the control of Scer\GAL4^{RhoGAP18B-NP1514} specifically in the adult (using the heat sensitive Scer\GAL80^ts.αTub84B allele to limit expression) does rescue the sensitivity of Arf51F^KG02753/Arf51F^EP2612 flies to ethanol-induced sedation.

(Peru Y Colón de Portugal et al., 2012)

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

17076

w¹¹¹⁸; P{EP}Arf6^EP2612/CyO

Notes on Origin

Discoverer

Separable from: a lethal mutation on the same chromosome.

(Dyer et al., 2007)

Selected as: a P{EP} insertion line that modifies the pnr^MD237/+ phenotype when expressed using Scer\GAL4^pnr-MD237.

(Pena-Rangel et al., 2002)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (5)

Reported As

Symbol Synonym

Arf51F^EP2612

Arf6^EP2612

Arf6^EP

(Gonzalez et al., 2018)

Arf6^P2

(Gonzalez et al., 2018, Acevedo et al., 2015, Peru Y Colón de Portugal et al., 2012)

EP2612

(Peru Y Colón de Portugal et al., 2012)

Name Synonyms

Secondary FlyBase IDs

References (8)

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