FlyBase Allele Report: Dmel\htt[GD14943]

General Information

Symbol

Dmel\htt^GD14943

Species

D. melanogaster

Name

FlyBase ID

FBal0209709

Feature type

allele

Associated gene

Dmel\htt

Associated Insertion(s)

Carried in Construct

P{GD14943}

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

(Dickson et al., 2007.7.18)

Progenitor genotype

Carried in construct

P{GD14943}

Cytology

Description

UASt regulatory sequences drive expression of an inverted repeat.

(Dickson et al., 2007.7.18)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

dsRNA-GD14943

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

(Neely et al., 2010)

viable, with Scer\GAL4^Mef2.PR

(Schnorrer et al., 2010)

viable, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

viable, with Scer\GAL4^tin.CΔ4

(Neely et al., 2010)

Phenotype Manifest In

mitotic spindle | third instar larval stage, with Scer\GAL4^insc.PU

(Godin et al., 2010)

neuroblast | third instar larval stage, with Scer\GAL4^insc.PU

(Godin et al., 2010)

Detailed Description

Statement

Reference

Expression of htt^GD14943 under the control of either Scer\GAL4^en.PU or Scer\GAL4^nub.PK does not lead to any obvious adult wing defects.

(Dietz et al., 2015)

Expression of htt^GD14943 under the control of Scer\GAL4^insc.PU results in mitotic spindle positioning defects in third instar larval neuroblasts: in contrast to wild-type controls, a significant proportion of cells show spindles with the angle between centrosomal pole and center of the mira protein crescent higher than the 15[o].

(Godin et al., 2010)

Adults expressing htt^GD14943 under the control of Scer\GAL4^elav.PLu (in the presence of Dcr-2^Scer\UAS.cDa to increase the efficiency of RNAi) do not show a significant defect in avoidance of noxious temperature (46[o]C) compared to control flies.

(Neely et al., 2010)

External Data

Linkouts

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development

14943

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhancer of

Statement

Reference

Scer\GAL4^en.PU/htt^GD14943 is an enhancer of visible phenotype of Scer\GAL4^en.PU, Su(var)3-3^GD9296

(Dietz et al., 2015)

Scer\GAL4^nub.PK/htt^GD14943 is an enhancer of visible | adult stage phenotype of Scer\GAL4^nub.PK, Su(var)205^KK110828

(Dietz et al., 2015)

htt^GD14943, Scer\GAL4^nub.PK is an enhancer of visible | adult stage phenotype of HP1b^KK108772, Scer\GAL4^nub.PK

(Dietz et al., 2015)

htt^GD14943, Scer\GAL4^en.PU is an enhancer of visible | adult stage phenotype of HP1b^KK108772, Scer\GAL4^en.PU

(Dietz et al., 2015)

htt^GD14943, Scer\GAL4^GMR.PF is an enhancer of visible | adult stage phenotype of E2f1^RNAi.UAS.cJa, Scer\GAL4^GMR.PF

(Dietz et al., 2015)

htt^GD14943, Scer\GAL4^en.PU is an enhancer of visible | adult stage phenotype of Jarid2^JF02081, Scer\GAL4^en.PU

(Dietz et al., 2015)

Scer\GAL4^en.PU/htt^GD14943 is an enhancer of visible | adult stage phenotype of Scer\GAL4^en.PU, ash1^JF01498

(Dietz et al., 2015)

htt^GD14943, Scer\GAL4^en.PU is an enhancer of visible | adult stage phenotype of Mt2^GD5033, Scer\GAL4^en.PU

(Dietz et al., 2015)

Scer\GAL4^nub.PK/htt^GD14943 is an enhancer of visible | adult stage phenotype of Scer\GAL4^nub.PK, brm^HM04019

(Dietz et al., 2015)

htt^GD14943/Scer\GAL4^ey.PH is an enhancer of visible | adult stage phenotype of Scer\GAL4^ey.PH, brm^{K804R.UAS.Tag:HA}

(Dietz et al., 2015)

htt^GD14943/Scer\GAL4^ey.PH is an enhancer of abnormal size | adult stage phenotype of Scer\GAL4^ey.PH, brm^{K804R.UAS.Tag:HA}

(Dietz et al., 2015)

Scer\GAL4^en.PU/htt^GD14943 is an enhancer of visible | adult stage phenotype of Scer\GAL4^en.PU, Snr1^GD4140

(Dietz et al., 2015)

htt^GD14943, Scer\GAL4^ey.PH is an enhancer of visible | adult stage phenotype of HDAC1^JF01401, Scer\GAL4^ey.PH

(Dietz et al., 2015)

Scer\GAL4^en.PU/htt^GD14943 is an enhancer of visible | adult stage phenotype of Scer\GAL4^en.PU, gpp^JF01283

(Dietz et al., 2015)

htt^GD14943, Scer\GAL4^nub.PK is an enhancer of visible | adult stage phenotype of Rga^GL00386, Scer\GAL4^nub.PK

(Dietz et al., 2015)

htt^GD14943, Scer\GAL4^en.PU is an enhancer of visible | adult stage phenotype of Rga^GL00386, Scer\GAL4^en.PU

(Dietz et al., 2015)

Scer\GAL4^nub.PK/htt^GD14943 is an enhancer of visible | adult stage phenotype of Scer\GAL4^nub.PK, not^JF03152

(Dietz et al., 2015)

Scer\GAL4^nub.PK/htt^GD14943 is an enhancer of visible | adult stage phenotype of Scer\GAL4^nub.PK, esc^GD1240

(Dietz et al., 2015)

Scer\GAL4^en.PU/htt^GD14943 is an enhancer of visible | adult stage phenotype of Scer\GAL4^en.PU, esc^GD1240

(Dietz et al., 2015)

Scer\GAL4^en.PU/htt^GD14943 is an enhancer of visible | adult stage phenotype of Scer\GAL4^en.PU, esc^JF01403

(Dietz et al., 2015)

Scer\GAL4^en.PU/htt^GD14943 is an enhancer of visible | adult stage phenotype of Scer\GAL4^en.PU, Sin3A^HMS00359

(Dietz et al., 2015)

Scer\GAL4^nub.PK/htt^GD14943 is an enhancer of visible | adult stage phenotype of Scer\GAL4^nub.PK, Set1^GD12893

(Dietz et al., 2015)

Scer\GAL4^en.PU/htt^GD14943 is an enhancer of visible | adult stage phenotype of Scer\GAL4^en.PU, Set1^GD12893

(Dietz et al., 2015)

htt^GD14943, Scer\GAL4^nub.PK is an enhancer of visible | adult stage phenotype of HDAC1^JF01401, Scer\GAL4^nub.PK

(Dietz et al., 2015)

htt^GD14943, Scer\GAL4^en.PU is an enhancer of visible | adult stage phenotype of HDAC6^HMS00077, Scer\GAL4^en.PU

(Dietz et al., 2015)

Suppressor of

Statement

Reference

htt^GD14943, Scer\GAL4^ey.PH is a suppressor of visible | adult stage phenotype of E(Pc)^JF03101, Scer\GAL4^ey.PH

(Dietz et al., 2015)

htt^GD14943, Scer\GAL4^GMR.PF is a suppressor of visible | adult stage phenotype of E(Pc)^JF03101, Scer\GAL4^GMR.PF

(Dietz et al., 2015)

htt^GD14943, Scer\GAL4^nub.PK is a suppressor of visible | adult stage phenotype of HP1b^KK108772, Scer\GAL4^nub.PK

(Dietz et al., 2015)

htt^GD14943, Scer\GAL4^en.PU is a suppressor of visible | adult stage phenotype of HP1b^KK108772, Scer\GAL4^en.PU

(Dietz et al., 2015)

htt^GD14943, Scer\GAL4^nub.PK is a suppressor of visible | adult stage phenotype of Orc2^KK102852, Scer\GAL4^nub.PK

(Dietz et al., 2015)

htt^GD14943, Scer\GAL4^nub.PK is a suppressor of visible | female | adult stage phenotype of Kdm5^HM05155, Scer\GAL4^nub.PK

(Dietz et al., 2015)

htt^GD14943, Scer\GAL4^nub.PK is a suppressor of visible | adult stage phenotype of Kdm5^KK102745, Scer\GAL4^nub.PK

(Dietz et al., 2015)

htt^GD14943, Scer\GAL4^nub.PK is a suppressor of visible | female | adult stage phenotype of Kdm5^KK102745, Scer\GAL4^nub.PK

(Dietz et al., 2015)

htt^GD14943, Scer\GAL4^en.PU is a suppressor | partially of visible | adult stage phenotype of Iswi^HMS00628, Scer\GAL4^en.PU

(Dietz et al., 2015)

Scer\GAL4^en.PU/htt^GD14943 is a suppressor | partially of visible | adult stage phenotype of Scer\GAL4^en.PU, osa^JF01207

(Dietz et al., 2015)

htt^GD14943, Scer\GAL4^nub.PK is a suppressor | partially of visible | adult stage phenotype of Pcl^HMS00896, Scer\GAL4^nub.PK

(Dietz et al., 2015)

htt^GD14943, Scer\GAL4^nub.PK is a suppressor of visible | adult stage phenotype of Caf1-55^HM04021, Scer\GAL4^nub.PK

(Dietz et al., 2015)

htt^GD14943/Scer\GAL4^ey.PH is a suppressor of visible | adult stage phenotype of Scer\GAL4^ey.PH, Sin3A^HMS00359

(Dietz et al., 2015)

Scer\GAL4^en.PU/htt^GD14943 is a suppressor of visible | adult stage phenotype of Scer\GAL4^en.PU, Sin3A^HMS00359

(Dietz et al., 2015)

htt^GD14943, Scer\GAL4^ey.PH is a suppressor of visible | adult stage phenotype of E(Pc)^GL00169, Scer\GAL4^ey.PH

(Dietz et al., 2015)

Scer\GAL4^en.PU/htt^GD14943 is a suppressor of visible | adult stage phenotype of Scer\GAL4^en.PU, Su(var)205^KK110828

(Dietz et al., 2015)

Scer\GAL4^nub.PK/htt^GD14943 is a suppressor of visible | adult stage phenotype of Scer\GAL4^nub.PK, Su(var)205^KK110828

(Dietz et al., 2015)

Other

Statement

Reference

Scer\GAL4^en.PU, gpp^JF01284, htt^GD14943 has visible | adult stage phenotype

(Dietz et al., 2015)

Kdm3^HMS00775, Scer\GAL4^en.PU, htt^GD14943 has visible | adult stage phenotype

(Dietz et al., 2015)

Jarid2^HMS00679, Scer\GAL4^en.PU, htt^GD14943 has visible | adult stage phenotype

(Dietz et al., 2015)

Asx^JF01708, Scer\GAL4^en.PU, htt^GD14943 has visible | adult stage phenotype

(Dietz et al., 2015)

Asx^KK102222, Scer\GAL4^en.PU, htt^GD14943 has visible | adult stage phenotype

(Dietz et al., 2015)

Phenotype Manifest In

Enhancer of

Statement

Reference

Scer\GAL4^en.PU/htt^GD14943 is an enhancer of wing vein phenotype of Scer\GAL4^en.PU, Su(var)3-3^GD9296

(Dietz et al., 2015)

htt^GD14943, Scer\GAL4^nub.PK is an enhancer of wing vein phenotype of HP1b^KK108772, Scer\GAL4^nub.PK

(Dietz et al., 2015)

htt^GD14943, Scer\GAL4^en.PU is an enhancer of wing vein phenotype of HP1b^KK108772, Scer\GAL4^en.PU

(Dietz et al., 2015)

htt^GD14943, Scer\GAL4^GMR.PF is an enhancer of eye phenotype of E2f1^RNAi.UAS.cJa, Scer\GAL4^GMR.PF

(Dietz et al., 2015)

htt^GD14943, Scer\GAL4^en.PU is an enhancer of wing vein phenotype of Jarid2^JF02081, Scer\GAL4^en.PU

(Dietz et al., 2015)

Scer\GAL4^en.PU/htt^GD14943 is an enhancer of ovary phenotype of Scer\GAL4^Act5C.PI, Su(var)3-3^GD9296

(Dietz et al., 2015)

Scer\GAL4^en.PU/htt^GD14943 is an enhancer of wing phenotype of Scer\GAL4^en.PU, ash1^JF01498

(Dietz et al., 2015)

htt^GD14943, Scer\GAL4^en.PU is an enhancer of wing phenotype of Mt2^GD5033, Scer\GAL4^en.PU

(Dietz et al., 2015)

Scer\GAL4^nub.PK/htt^GD14943 is an enhancer of wing blade phenotype of Scer\GAL4^nub.PK, brm^HM04019

(Dietz et al., 2015)

htt^GD14943/Scer\GAL4^ey.PH is an enhancer of eye phenotype of Scer\GAL4^ey.PH, brm^{K804R.UAS.Tag:HA}

(Dietz et al., 2015)

Scer\GAL4^en.PU/htt^GD14943 is an enhancer of wing phenotype of Scer\GAL4^en.PU, Snr1^GD4140

(Dietz et al., 2015)

Scer\GAL4^en.PU/htt^GD14943 is an enhancer of wing phenotype of Scer\GAL4^en.PU, gpp^JF01283

(Dietz et al., 2015)

htt^GD14943, Scer\GAL4^nub.PK is an enhancer of wing vein phenotype of Rga^GL00386, Scer\GAL4^nub.PK

(Dietz et al., 2015)

htt^GD14943, Scer\GAL4^en.PU is an enhancer of wing vein phenotype of Rga^GL00386, Scer\GAL4^en.PU

(Dietz et al., 2015)

Scer\GAL4^nub.PK/htt^GD14943 is an enhancer of wing phenotype of Scer\GAL4^nub.PK, not^JF03152

(Dietz et al., 2015)

Scer\GAL4^nub.PK/htt^GD14943 is an enhancer of wing phenotype of Scer\GAL4^nub.PK, esc^GD1240

(Dietz et al., 2015)

htt^GD14943, Scer\GAL4^ey.PH is an enhancer of eye phenotype of HDAC1^JF01401, Scer\GAL4^ey.PH

(Dietz et al., 2015)

Scer\GAL4^en.PU/htt^GD14943 is an enhancer of wing phenotype of Scer\GAL4^en.PU, esc^GD1240

(Dietz et al., 2015)

Scer\GAL4^en.PU/htt^GD14943 is an enhancer of wing phenotype of Scer\GAL4^en.PU, esc^JF01403

(Dietz et al., 2015)

Scer\GAL4^en.PU/htt^GD14943 is an enhancer of posterior crossvein phenotype of Scer\GAL4^en.PU, Sin3A^HMS00359

(Dietz et al., 2015)

Scer\GAL4^nub.PK/htt^GD14943 is an enhancer of wing phenotype of Scer\GAL4^nub.PK, Set1^GD12893

(Dietz et al., 2015)

Scer\GAL4^en.PU/htt^GD14943 is an enhancer of wing phenotype of Scer\GAL4^en.PU, Set1^GD12893

(Dietz et al., 2015)

htt^GD14943, Scer\GAL4^nub.PK is an enhancer of wing blade phenotype of HDAC1^JF01401, Scer\GAL4^nub.PK

(Dietz et al., 2015)

htt^GD14943, Scer\GAL4^en.PU is an enhancer of wing vein phenotype of HDAC6^HMS00077, Scer\GAL4^en.PU

(Dietz et al., 2015)

Scer\GAL4^en.PU/htt^GD14943 is an enhancer of wing posterior compartment phenotype of Scer\GAL4^en.PU, Su(var)205^KK110828

(Dietz et al., 2015)

Suppressor of

Statement

Reference

htt^GD14943, Scer\GAL4^nub.PK is a suppressor of wing margin phenotype of HP1b^KK108772, Scer\GAL4^nub.PK

(Dietz et al., 2015)

htt^GD14943, Scer\GAL4^en.PU is a suppressor of wing margin phenotype of HP1b^KK108772, Scer\GAL4^en.PU

(Dietz et al., 2015)

htt^GD14943, Scer\GAL4^nub.PK is a suppressor of wing phenotype of Orc2^KK102852, Scer\GAL4^nub.PK

(Dietz et al., 2015)

htt^GD14943, Scer\GAL4^nub.PK is a suppressor of wing blade | female phenotype of Kdm5^HM05155, Scer\GAL4^nub.PK

(Dietz et al., 2015)

htt^GD14943, Scer\GAL4^ey.PH is a suppressor of eye phenotype of E(Pc)^JF03101, Scer\GAL4^ey.PH

(Dietz et al., 2015)

htt^GD14943, Scer\GAL4^nub.PK is a suppressor of posterior crossvein phenotype of Kdm5^KK102745, Scer\GAL4^nub.PK

(Dietz et al., 2015)

htt^GD14943, Scer\GAL4^nub.PK is a suppressor of wing blade | female phenotype of Kdm5^KK102745, Scer\GAL4^nub.PK

(Dietz et al., 2015)

htt^GD14943, Scer\GAL4^en.PU is a suppressor | partially of wing blade phenotype of Iswi^HMS00628, Scer\GAL4^en.PU

(Dietz et al., 2015)

Scer\GAL4^en.PU/htt^GD14943 is a suppressor | partially of wing phenotype of Scer\GAL4^en.PU, osa^JF01207

(Dietz et al., 2015)

htt^GD14943, Scer\GAL4^nub.PK is a suppressor | partially of wing phenotype of Pcl^HMS00896, Scer\GAL4^nub.PK

(Dietz et al., 2015)

htt^GD14943, Scer\GAL4^nub.PK is a suppressor of wing blade phenotype of Caf1-55^HM04021, Scer\GAL4^nub.PK

(Dietz et al., 2015)

htt^GD14943, Scer\GAL4^GMR.PF is a suppressor of eye phenotype of E(Pc)^JF03101, Scer\GAL4^GMR.PF

(Dietz et al., 2015)

htt^GD14943/Scer\GAL4^ey.PH is a suppressor of eye phenotype of Scer\GAL4^ey.PH, Sin3A^HMS00359

(Dietz et al., 2015)

Scer\GAL4^en.PU/htt^GD14943 is a suppressor of wing vein L5 phenotype of Scer\GAL4^en.PU, Sin3A^HMS00359

(Dietz et al., 2015)

htt^GD14943, Scer\GAL4^ey.PH is a suppressor of eye phenotype of E(Pc)^GL00169, Scer\GAL4^ey.PH

(Dietz et al., 2015)

Scer\GAL4^en.PU/htt^GD14943 is a suppressor of wing margin phenotype of Scer\GAL4^en.PU, Su(var)205^KK110828

(Dietz et al., 2015)

Scer\GAL4^nub.PK/htt^GD14943 is a suppressor of wing margin phenotype of Scer\GAL4^nub.PK, Su(var)205^KK110828

(Dietz et al., 2015)

Other

Statement

Reference

Scer\GAL4^en.PU, Su(var)205^KK110828, htt^GD14943 has wing vein phenotype

(Dietz et al., 2015)

Kdm3^HMS00775, Scer\GAL4^en.PU, htt^GD14943 has wing vein phenotype

(Dietz et al., 2015)

Jarid2^HMS00679, Scer\GAL4^en.PU, htt^GD14943 has wing vein phenotype

(Dietz et al., 2015)

Asx^JF01708, Scer\GAL4^en.PU, htt^GD14943 has wing vein phenotype

(Dietz et al., 2015)

Asx^KK102222, Scer\GAL4^en.PU, htt^GD14943 has wing vein phenotype

(Dietz et al., 2015)

Scer\GAL4^en.PU, gpp^JF01284, htt^GD14943 has wing vein phenotype

(Dietz et al., 2015)

Additional Comments

Genetic Interactions

Statement

Reference

The ovarian defects observed in females expressing Su(var)3-3^GD9296 under the control of Scer\GAL4^Act5C.PI as well as the ectopic venation phenotype induced by Scer\GAL4^en.PU-driven expression of Su(var)3-3^GD9296 are enhanced by co-expression of htt^GD14943.

Co-expression with htt^GD14943 enhances, to a varying degree, the adult wing phenotypes induced by expression of any of the following: HDAC1^JF01401, HDAC6^HMS00077, Su(var)3-3^GD9296, Jarid2^JF02081, ash1^JF01498, Snr1^GD4140, not^JF03152, esc^GD1240, Set1^GD12893, esc^JF01403, Mt2^GD5033, brm^HM04019, gpp^JF01283, or Rga^GL00386 (controlled by Scer\GAL4^nub.PK or Scer\GAL4^en.PU).

Adult flies co-expressing htt^GD14943 and any of the following: JHDM2^HMS00775, Jarid2^HMS00679, Asx^JF01708, Asx^KK102222, gpp^JF01284 and Su(var)205^KK110828 under the control of Scer\GAL4^en.PU display ectopic wing vein phenotype.

Co-expression of htt^GD14943 suppresses the adult eye phenotype induced by expression of E(Pc)^JF03101 (under the control of either Scer\GAL4^ey.PH or Scer\GAL4^GMR.PF) or E(Pc)^GL00169 (driven by Scer\GAL4^ey.PH), whereas it enhances the eye defects observed upon expression of HDAC1^JF01401 or brm^{K804R.Scer\UAS.T:Ivir\HA1} (with Scer\GAL4^ey.PH) or E2f1^{dsRNA.Scer\UAS.cJa} (with Scer\GAL4^GMR.PF).

The various wing defects induced by expression of either HP1b^KK108772 or Su(var)205^KK110828 under the control of Scer\GAL4^nub.PK or Scer\GAL4^en.PU are both enhanced and suppressed by co-expression of htt^GD14943 - the ectopic venation phenotype is enhanced while the wing notches are suppressed.

The adult eye phenotype induced by expression of Sin3A^HMS00359 under the control of Scer\GAL4^ey.PH is suppressed by co-expression of htt^GD14943, the wing phenotype observed with the Scer\GAL4^en.PU driver is both enhanced and suppressed by co-expression of htt^GD14943 - it suppresses the L5 wing vein defect but enhances the ectopic posterior crossvein phenotype.

(Dietz et al., 2015)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 1 )

Linkouts

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development

14943

Synonyms and Secondary IDs (1)

Reported As

Symbol Synonym

htt^GD14943

Name Synonyms

Secondary FlyBase IDs

References (8)

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