FlyBase Allele Report: Dmel\Ctr1A[GD16726]

General Information

Symbol

Dmel\Ctr1A^GD16726

Species

D. melanogaster

Name

FlyBase ID

FBal0206701

Feature type

allele

Associated gene

Dmel\Ctr1A

Associated Insertion(s)

Carried in Construct

P{GD16726}

Also Known As

Ctr1A^RNAi, UAS-Ctr1A^RNAi

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

(Dickson et al., 2007.7.18)

Progenitor genotype

Carried in construct

P{GD16726}

Cytology

Description

UASt regulatory sequences drive expression of an inverted repeat.

(Dickson et al., 2007.7.18)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

dsRNA-GD16726

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

adult abdomen, with Scer\GAL4^pnr-MD237

(Zhang et al., 2020)

adult cuticle, with Scer\GAL4^pnr-MD237

(Binks et al., 2010, Norgate et al., 2010)

adult thorax, with Scer\GAL4^pnr-MD237

(Zhang et al., 2020)

eye, with Scer\GAL4^GMR.PFa

(Mercer and Burke, 2016, Binks et al., 2010)

mesothoracic tergum, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

ommatidium, with Scer\GAL4^GMR.PFa

(Binks et al., 2010)

trichogen cell, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

wing disc | third instar larval stage, with Scer\GAL4^pnr-MD237

(Zhang et al., 2020)

Detailed Description

Statement

Reference

Expression of Ctr1A^GD16726 under the control of Scer\GAL4^pnr-MD237 leads to thoracic and abdominal hypopigmentation, and thoracic cleft when compared to controls.

(Zhang et al., 2020)

Expression of Ctr1A^GD16726 driven in the thoracic midline by Scer\GAL4^pnr-MD237 results in hypo-pigmentation in the thorax and abdomen. Expression of Ctr1A^GD16726 driven in the eye by Scer\GAL4^GMR.PFa causes a smaller flattened eye with necrotic patches, compared to controls.

(Mercer and Burke, 2016)

Expression of Ctr1A^GD16726 driven by Scer\GAL4^elav-C155 does not significantly affect larval lethality compared to controls.

(Mercer et al., 2016)

Expression of Ctr1A^GD16726 under the control of Scer\GAL4^CCAP.PP does not cause any significant increase in the number of adults with unexpanded wings, as compared to controls.

(Hwang et al., 2014)

Expression of Ctr1A^GD16726 under the control of Scer\GAL4^pnr-MD237 results in a hypopigmentation phenotype in the adult dorsal cuticle of the thorax and abdomen (in the domain of Scer\GAL4 expression), with the degree of hypopigmentation depending on the Ctr1A^GD16726 line used.

Expression of Ctr1A^GD16726 under the control of Scer\GAL4^GMR.PFa results in a sunken eye phenotype, with a reduction in eye size, reduction in the number of ommatidia, folding of the normally smooth eye surface and occasional complete loss of ommatidia in some places.

(Binks et al., 2010)

Adults expressing Ctr1A^GD16726 under the control of Scer\GAL4^elav.PLu (in the presence of Dcr-2^Scer\UAS.cDa to increase the efficiency of RNAi) do not show a significant defect in avoidance of noxious temperature (46[o]C) compared to control flies.

(Neely et al., 2010)

Flies expressing Ctr1A^GD16726 under the control of Scer\GAL4^pnr-MD237 show decreased pigmentation.

(Norgate et al., 2010)

Depending on the insertion line used, expression under the control of Scer\GAL4^Mef2.PR can result in viable flies or late pupal lethality.

(Schnorrer et al., 2010)

Expression under the control of Scer\GAL4^pnr-MD237 may result in lethality, depending on the insertion line used.

Expression under the control of Scer\GAL4^pnr-MD237 results in a colour difference between the central Scer\GAL4^pnr-MD237 expression domain of the notum and the surrounding lateral region in 90-100% of the Scer\GAL4^pnr-MD237 expression domain.

Expression under the control of Scer\GAL4^pnr-MD237 results in bristle morphology defects on the notum in 80-90% of the Scer\GAL4^pnr-MD237 expression domain.

(Mummery-Widmer et al., 2009)

External Data

Linkouts

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development

16726

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Ctr1A^GD16726, Scer\GAL4^GMR.PFa has visible phenotype, enhanceable by Grx1^GD10587, Scer\GAL4^GMR.PFa

(Mercer and Burke, 2016)

Ctr1A^GD16726, Scer\GAL4^pnr-MD237 has abnormal body color | adult stage phenotype, enhanceable by Ctr1B^GD2859, Scer\GAL4^pnr-MD237

(Binks et al., 2010)

NOT Enhanced by

Statement

Reference

Ctr1A^GD16726, Scer\GAL4^pnr-MD237 has visible | adult stage phenotype, non-enhanceable by slmb^UAS.cGa, Scer\GAL4^pnr-MD237

(Zhang et al., 2020)

Ctr1A^GD16726, Scer\GAL4^pnr-MD237 has visible | adult stage phenotype, non-enhanceable by slmb^GD10682, Scer\GAL4^pnr-MD237

(Zhang et al., 2020)

Suppressed by

Statement

Reference

Ctr1A^GD16726, Scer\GAL4^pnr-MD237 has abnormal body color | adult stage phenotype, suppressible by Ctr1B^UAS.Tag:FLAG, Scer\GAL4^pnr-MD237

(Binks et al., 2010)

NOT suppressed by

Statement

Reference

Ctr1A^GD16726, Scer\GAL4^pnr-MD237 has visible | adult stage phenotype, non-suppressible by slmb^UAS.cGa, Scer\GAL4^pnr-MD237

(Zhang et al., 2020)

Ctr1A^GD16726, Scer\GAL4^pnr-MD237 has visible | adult stage phenotype, non-suppressible by slmb^GD10682, Scer\GAL4^pnr-MD237

(Zhang et al., 2020)

Ctr1A^GD16726, Scer\GAL4^GMR.PFa has visible phenotype, non-suppressible by ATP7^GD3322, Scer\GAL4^GMR.PFa

(Binks et al., 2010)

Ctr1A^GD16726, Scer\GAL4^GMR.PFa has visible phenotype, non-suppressible by ATP7^UAS.Tag:FLAG, Scer\GAL4^GMR.PFa

(Binks et al., 2010)

Enhancer of

Statement

Reference

Ctr1A^GD16726, Scer\GAL4^pnr-MD237 is an enhancer of abnormal body color | adult stage phenotype of Scer\GAL4^pnr-MD237, Vhl^KK111257

(Zhang et al., 2021)

Ctr1A^GD16726, Scer\GAL4^GMR.PFa is an enhancer of visible phenotype of Grx1^GD10587, Scer\GAL4^GMR.PFa

(Mercer and Burke, 2016)

Ctr1A^GD16726, Scer\GAL4^pnr-MD237 is an enhancer of visible phenotype of Grx1^GD10587, Scer\GAL4^pnr-MD237

(Mercer and Burke, 2016)

Ctr1A^GD16726, Scer\GAL4^elav-C155 is an enhancer of majority die during larval stage phenotype of Gclc^GD9767, Scer\GAL4^elav-C155

(Mercer et al., 2016)

Ctr1A^GD16726, Scer\GAL4^pnr-MD237 is an enhancer of visible phenotype of Scer\GAL4^pnr-MD237, Syx5^GD1743, Syx5^UAS.Tag:MYC

(Norgate et al., 2010)

NOT Enhancer of

Statement

Reference

Ctr1A^GD16726, Scer\GAL4^pnr-MD237 is a non-enhancer of visible | adult stage phenotype of Scer\GAL4^pnr-MD237, slmb^GD10682

(Zhang et al., 2020)

Suppressor of

Statement

Reference

Ctr1A^GD16726, Scer\GAL4^pnr-MD237 is a suppressor of abnormal body color | adult stage phenotype of Scer\GAL4^pnr-MD237, Vhl^KK111257

(Zhang et al., 2021)

NOT Suppressor of

Statement

Reference

Ctr1A^GD16726, Scer\GAL4^pnr-MD237 is a non-suppressor of visible | adult stage phenotype of Scer\GAL4^pnr-MD237, slmb^GD10682

(Zhang et al., 2020)

Other

Statement

Reference

ATP7^UAS.Tag:FLAG, Ctr1A^GD16726, Scer\GAL4^CCAP.PP has visible phenotype

(Hwang et al., 2014)

Phenotype Manifest In

Enhanced by

Statement

Reference

Ctr1A^GD16726, Scer\GAL4^GMR.PFa has eye phenotype, enhanceable by Grx1^GD10587, Scer\GAL4^GMR.PFa

(Mercer and Burke, 2016)

Ctr1A^GD16726, Scer\GAL4^pnr-MD237 has adult cuticle phenotype, enhanceable by Ctr1B^GD2859, Scer\GAL4^pnr-MD237

(Binks et al., 2010)

NOT Enhanced by

Statement

Reference

Ctr1A^GD16726, Scer\GAL4^pnr-MD237 has adult thorax phenotype, non-enhanceable by slmb^UAS.cGa, Scer\GAL4^pnr-MD237

(Zhang et al., 2020)

Ctr1A^GD16726, Scer\GAL4^pnr-MD237 has adult thorax phenotype, non-enhanceable by slmb^GD10682, Scer\GAL4^pnr-MD237

(Zhang et al., 2020)

Suppressed by

Statement

Reference

Ctr1A^GD16726, Scer\GAL4^pnr-MD237 has adult cuticle phenotype, suppressible by Ctr1B^UAS.Tag:FLAG, Scer\GAL4^pnr-MD237

(Binks et al., 2010)

NOT suppressed by

Statement

Reference

Ctr1A^GD16726, Scer\GAL4^pnr-MD237 has adult thorax phenotype, non-suppressible by slmb^UAS.cGa, Scer\GAL4^pnr-MD237

(Zhang et al., 2020)

Ctr1A^GD16726, Scer\GAL4^pnr-MD237 has adult thorax phenotype, non-suppressible by slmb^GD10682, Scer\GAL4^pnr-MD237

(Zhang et al., 2020)

Ctr1A^GD16726, Scer\GAL4^GMR.PFa has eye phenotype, non-suppressible by ATP7^GD3322, Scer\GAL4^GMR.PFa

(Binks et al., 2010)

Ctr1A^GD16726, Scer\GAL4^GMR.PFa has eye phenotype, non-suppressible by ATP7^UAS.Tag:FLAG, Scer\GAL4^GMR.PFa

(Binks et al., 2010)

Enhancer of

Statement

Reference

Ctr1A^GD16726, Scer\GAL4^pnr-MD237 is an enhancer of adult external abdomen phenotype of Scer\GAL4^pnr-MD237, Vhl^KK111257

(Zhang et al., 2021)

Ctr1A^GD16726, Scer\GAL4^pnr-MD237 is an enhancer of adult thorax phenotype of Grx1^GD10587, Scer\GAL4^pnr-MD237

(Mercer and Burke, 2016)

Ctr1A^GD16726, Scer\GAL4^pnr-MD237 is an enhancer of scutellum phenotype of Grx1^GD10587, Scer\GAL4^pnr-MD237

(Mercer and Burke, 2016)

Ctr1A^GD16726, Scer\GAL4^GMR.PFa is an enhancer of eye phenotype of Grx1^GD10587, Scer\GAL4^GMR.PFa

(Mercer and Burke, 2016)

Ctr1A^GD16726, Scer\GAL4^pnr-MD237 is an enhancer of adult cuticle phenotype of Scer\GAL4^pnr-MD237, Syx5^GD1743, Syx5^UAS.Tag:MYC

(Norgate et al., 2010)

NOT Enhancer of

Statement

Reference

Ctr1A^GD16726, Scer\GAL4^pnr-MD237 is a non-enhancer of adult thorax phenotype of Scer\GAL4^pnr-MD237, slmb^GD10682

(Zhang et al., 2020)

Suppressor of

Statement

Reference

Ctr1A^GD16726, Scer\GAL4^pnr-MD237 is a suppressor of adult external thorax phenotype of Scer\GAL4^pnr-MD237, Vhl^KK111257

(Zhang et al., 2021)

NOT Suppressor of

Statement

Reference

Ctr1A^GD16726, Scer\GAL4^pnr-MD237 is a non-suppressor of adult thorax phenotype of Scer\GAL4^pnr-MD237, slmb^GD10682

(Zhang et al., 2020)

Other

Statement

Reference

ATP7^UAS.Tag:FLAG, Ctr1A^GD16726, Scer\GAL4^CCAP.PP has wing phenotype

(Hwang et al., 2014)

Additional Comments

Genetic Interactions

Statement

Reference

Co-expression of Ctr1A^GD16726 enhances the thoracic cleft and reduced scutellum seen in flies with expression of Grx1^GD10587 driven by Scer\GAL4^pnr-MD237.

Co-expression of Grx1^GD10587 and Ctr1A^GD16726 driven by Scer\GAL4^GMR.PFa enhances eye phenotypes seen with expression of either alone, with a smaller glazed eye with patches of necrosis and some loss of pigmentation.

(Mercer and Burke, 2016)

Co-expression of Ctr1A^GD16726 significantly enhances larval lethality seen with expression of Gclc^GD9767 driven by Scer\GAL4^elav-C155.

(Mercer et al., 2016)

Co-expression of Ctr1A^GD16726 and ATP7^{Scer\UAS.T:Zzzz\FLAG} under the control of Scer\GAL4^CCAP.PP causes a highly penetrant unexpanded wing phenotype, as compared to controls.

(Hwang et al., 2014)

Co-expression of Ctr1B^GD2859 enhances the cuticle hypopigmentation phenotype caused by expression of a weak Ctr1A^GD16726 line under the control of Scer\GAL4^pnr-MD237.

Co-expression of Ctr1B^{Scer\UAS.T:Zzzz\FLAG} rescues the cuticle hypopigmentation phenotype caused by expression of a weak Ctr1A^GD16726 line under the control of Scer\GAL4^pnr-MD237.

Co-expression of ATP7^GD3322 has no effect on the eye phenotype caused by expression of Ctr1A^GD16726 under the control of Scer\GAL4^GMR.PFa.

Co-expression of Ctr1A^GD16726 and Ctr1B^{Scer\UAS.T:Zzzz\FLAG} under the control of Scer\GAL4^GMR.PFa results in flies with relatively normal eyes.

Co-expression of ATP7^{Scer\UAS.T:Zzzz\FLAG} has no effect on the eye phenotype caused by expression of Ctr1A^GD16726 under the control of Scer\GAL4^GMR.PFa.

(Binks et al., 2010)

Co-expression of Ctr1A^GD16726 under the control of Scer\GAL4^pnr-MD237 exacerbates the Syx5^GD1743-induced hypopigmentation in flies also expressing Syx5^{Scer\UAS.T:Hsap\MYC}.

(Norgate et al., 2010)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

Ctr1A^GD16726 is rescued by Ctr1A^{dN.UAS.Tag:FLAG}/Scer\GAL4^pnr-MD237

(Binks et al., 2010)

Not rescued by

Ctr1A^GD16726 is not rescued by Ctr1A^{dN.UAS.Tag:FLAG}/Scer\GAL4^GMR.PFa

(Binks et al., 2010)

Comments

Co-expression of Ctr1A^{Scer\UAS.T:Zzzz\FLAG} rescues the cuticle hypopigmentation phenotype caused by expression of a weak Ctr1A^GD16726 line under the control of Scer\GAL4^pnr-MD237.

Co-expression of Ctr1A^{Scer\UAS.T:Zzzz\FLAG} does not rescue the eye phenotype caused by expression of Ctr1A^GD16726 under the control of Scer\GAL4^GMR.PFa.

(Binks et al., 2010)

Images (0)

Mutant

Wild-type

Stocks (1)

VDRC

v46757

w¹¹¹⁸; P{GD16726}v46757/TM3

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 1 )

Linkouts