FlyBase Allele Report: Dmel\Ctr1B[UAS.Tag:FLAG]

General Information

Symbol

Dmel\Ctr1B^UAS.Tag:FLAG

Species

D. melanogaster

Name

FlyBase ID

FBal0249313

Feature type

allele

Associated gene

Dmel\Ctr1B

Associated Insertion(s)

Carried in Construct

P{UAS-Ctr1B.FLAG}

Also Known As

UAS-Ctr1B^FLAG

Key Links

Transgenic product class

wild_type

(Binks et al., 2010)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

wild_type

(Binks et al., 2010)

Mutagen

in vitro construct

(Binks et al., 2010)

Progenitor genotype

Carried in construct

P{UAS-Ctr1B.FLAG}

Cytology

Description

UASt regulatory sequences drive expression of Ctr1B which is tagged at the C-terminal end with Tag:FLAG.

(Binks et al., 2010)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

Ctr1B

Tagged with

Tag:FLAG

epitope tag

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Disease

Evidence

References

model of Wilson disease

CEA

(Hwang et al., 2014)

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

chemical sensitive, with Scer\GAL4^CCAP.PP

(Hwang et al., 2014)

chemical sensitive | female, with Scer\GAL4^elav-C155

(Hwang et al., 2014)

partially lethal - majority die | male, with Scer\GAL4^elav-C155

(Hwang et al., 2014)

viable | female, with Scer\GAL4^elav-C155

(Hwang et al., 2014)

visible, with Scer\GAL4^CCAP.PP

(Hwang et al., 2014)

visible, with Scer\GAL4^GMR.PFa

(Binks et al., 2010)

Phenotype Manifest In

eye, with Scer\GAL4^GMR.PFa

(Binks et al., 2010)

wing, with Scer\GAL4^CCAP.PP

(Hwang et al., 2014)

Detailed Description

Statement

Reference

Expression of Ctr1B^{Scer\UAS.T:Zzzz\FLAG} driven in the thoracic midline by Scer\GAL4^pnr-MD237 does not cause significant observable effects.

(Mercer and Burke, 2016)

Expression of Ctr1B^{Scer\UAS.T:Zzzz\FLAG} under the control of Scer\GAL4^elav-C155 causes lethality at larval stages in males, but not females, as compared to controls, but supplementation with 1mM CuSO[[4]] results in complete female lethality and does not significantly change male lethality. Surviving adults do not display any unexpanded wing phenotype.

Expression of Ctr1B^{Scer\UAS.T:Zzzz\FLAG} under the control of Scer\GAL4^CCAP.PP leads to increased incidence of unexpanded wings, as compared to controls, and incidence increases to nearly 100% upon dietary supplementation with 1mM CuSO[[4]].

(Hwang et al., 2014)

Expression of Ctr1B^{Scer\UAS.T:Zzzz\FLAG} under the control of Scer\GAL4^GMR.PFa results in a mild dose- and temperature-dependent rough eye phenotype, which is exacerbated by increased dietary copper.

(Binks et al., 2010)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Ctr1B^UAS.Tag:FLAG, Scer\GAL4^GMR.PFa has visible phenotype, enhanceable by Ctr1A^{dN.UAS.Tag:FLAG}, Scer\GAL4^GMR.PFa

(Binks et al., 2010)

Suppressed by

Statement

Reference

Ctr1B^UAS.Tag:FLAG, Scer\GAL4^GMR.PFa has visible phenotype, suppressible by ATP7^UAS.Tag:FLAG, Scer\GAL4^GMR.PFa

(Binks et al., 2010)

NOT Enhancer of

Statement

Reference

Ctr1B^UAS.Tag:FLAG, Scer\GAL4^pnr-MD237 is a non-enhancer of visible phenotype of Grx1^GD10587, Scer\GAL4^pnr-MD237

(Mercer and Burke, 2016)

Suppressor of

Statement

Reference

Ctr1B^UAS.Tag:FLAG, Scer\GAL4^pnr-MD237 is a suppressor of abnormal body color | adult stage phenotype of Ctr1A^GD16726, Scer\GAL4^pnr-MD237

(Binks et al., 2010)

Ctr1B^UAS.Tag:FLAG, Scer\GAL4^pnr-MD237 is a suppressor of abnormal body color | adult stage phenotype of ATP7^UAS.Tag:FLAG, Scer\GAL4^pnr-MD237

(Binks et al., 2010)

NOT Suppressor of

Statement

Reference

Ctr1B^UAS.Tag:FLAG, Scer\GAL4^pnr-MD237 is a non-suppressor of visible phenotype of Grx1^GD10587, Scer\GAL4^pnr-MD237

(Mercer and Burke, 2016)

Other

Statement

Reference

ATP7^GD3322, Ctr1B^UAS.Tag:FLAG, Scer\GAL4^GMR.PFa has lethal | male phenotype

(Binks et al., 2010)

ATP7^GD3322, Ctr1B^UAS.Tag:FLAG, Scer\GAL4^GMR.PFa has visible | female phenotype

(Binks et al., 2010)

ATP7^GD3322, Ctr1B^UAS.Tag:FLAG, Scer\GAL4^GMR.PFa has abnormal eye color | female phenotype

(Binks et al., 2010)

Phenotype Manifest In

Enhanced by

Statement

Reference

Ctr1B^UAS.Tag:FLAG, Scer\GAL4^GMR.PFa has eye phenotype, enhanceable by Ctr1A^{dN.UAS.Tag:FLAG}, Scer\GAL4^GMR.PFa

(Binks et al., 2010)

Suppressed by

Statement

Reference

Ctr1B^UAS.Tag:FLAG, Scer\GAL4^GMR.PFa has eye phenotype, suppressible by ATP7^UAS.Tag:FLAG, Scer\GAL4^GMR.PFa

(Binks et al., 2010)

NOT Enhancer of

Statement

Reference

Ctr1B^UAS.Tag:FLAG, Scer\GAL4^pnr-MD237 is a non-enhancer of adult thorax phenotype of Grx1^GD10587, Scer\GAL4^pnr-MD237

(Mercer and Burke, 2016)

Ctr1B^UAS.Tag:FLAG, Scer\GAL4^pnr-MD237 is a non-enhancer of scutellum phenotype of Grx1^GD10587, Scer\GAL4^pnr-MD237

(Mercer and Burke, 2016)

Suppressor of

Statement

Reference

Ctr1B^UAS.Tag:FLAG, Scer\GAL4^pnr-MD237 is a suppressor of adult cuticle phenotype of Ctr1A^GD16726, Scer\GAL4^pnr-MD237

(Binks et al., 2010)

Ctr1B^UAS.Tag:FLAG, Scer\GAL4^pnr-MD237 is a suppressor of adult cuticle phenotype of ATP7^UAS.Tag:FLAG, Scer\GAL4^pnr-MD237

(Binks et al., 2010)

NOT Suppressor of

Statement

Reference

Ctr1B^UAS.Tag:FLAG, Scer\GAL4^pnr-MD237 is a non-suppressor of adult thorax phenotype of Grx1^GD10587, Scer\GAL4^pnr-MD237

(Mercer and Burke, 2016)

Ctr1B^UAS.Tag:FLAG, Scer\GAL4^pnr-MD237 is a non-suppressor of scutellum phenotype of Grx1^GD10587, Scer\GAL4^pnr-MD237

(Mercer and Burke, 2016)

Other

Statement

Reference

ATP7^GD3322, Ctr1B^UAS.Tag:FLAG, Scer\GAL4^GMR.PFa has eye | female phenotype

(Binks et al., 2010)

Additional Comments

Genetic Interactions

Statement

Reference

Co-expression of Ctr1B^{Scer\UAS.T:Zzzz\FLAG} does not alter Scer\GAL4^pnr-MD237>Grx1^GD10587 phenotypes.

(Mercer and Burke, 2016)

Co-expression of Ctr1B^{Scer\UAS.T:Zzzz\FLAG} rescues the cuticle hypopigmentation phenotype caused by expression of ATP7^{Scer\UAS.T:Zzzz\FLAG} under the control of Scer\GAL4^pnr-MD237.

Co-expression of Ctr1B^{Scer\UAS.T:Zzzz\FLAG} rescues the cuticle hypopigmentation phenotype caused by expression of a weak Ctr1A^GD16726 line under the control of Scer\GAL4^pnr-MD237.

Co-expression of Ctr1A^{Scer\UAS.T:Zzzz\FLAG} enhances the rough eye phenotype caused by expression of Ctr1B^{Scer\UAS.T:Zzzz\FLAG} under the control of Scer\GAL4^GMR.PFa.

Co-expression of ATP7^{Scer\UAS.T:Zzzz\FLAG} suppresses the rough eye phenotype caused by expression of Ctr1B^{Scer\UAS.T:Zzzz\FLAG} under the control of Scer\GAL4^GMR.PFa.

Co-expression of Ctr1B^{Scer\UAS.T:Zzzz\FLAG} and ATP7^GD3322 under the control of Scer\GAL4^GMR.PFa results in male lethality, while females have a severe rough eye phenotype together with loss of eye pigmentation.

Co-expression of Ctr1A^GD16726 and Ctr1B^{Scer\UAS.T:Zzzz\FLAG} under the control of Scer\GAL4^GMR.PFa results in flies with relatively normal eyes.

(Binks et al., 2010)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

Ctr1B^{Scer\UAS.T:Zzzz\FLAG}

Ctr1B^UAS.Tag:FLAG

Name Synonyms

Secondary FlyBase IDs

References (5)

Report Sections

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