FlyBase Allele Report: Dmel\ATP7[GD3322]

General Information

Symbol

Dmel\ATP7^GD3322

Species

D. melanogaster

Name

FlyBase ID

FBal0198609

Feature type

allele

Associated gene

Dmel\ATP7

Associated Insertion(s)

Carried in Construct

P{GD3322}

Also Known As

ATP7^RNAi, UAS-ATP7^RNAi

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

(Dickson et al., 2007.7.18)

Progenitor genotype

Carried in construct

P{GD3322}

Cytology

Description

UASt regulatory sequences drive expression of an inverted repeat.

(Dickson et al., 2007.7.18)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

dsRNA-GD3322

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

exacerbates Huntington's disease

modeled by Hsap\HTT^Q93.ex1.UAS

(Xiao et al., 2013)

ameliorates Menkes disease

modeled by slmb^GD10682

(Zhang et al., 2020)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal body color, with Scer\GAL4^pnr-MD237

(Mercer and Burke, 2016, Mummery-Widmer et al., 2009)

abnormal body color | adult stage, with Scer\GAL4^pnr-MD237

(Binks et al., 2010)

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

(Neely et al., 2010)

viable, with Scer\GAL4^Mef2.PR

(Schnorrer et al., 2010)

viable, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

viable, with Scer\GAL4^tin.CΔ4

(Neely et al., 2010)

visible, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

Phenotype Manifest In

adult cuticle, with Scer\GAL4^pnr-MD237

(Binks et al., 2010)

mesothoracic tergum, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

trichogen cell, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

Detailed Description

Statement

Reference

Expression of ATP7^GD3322 driven in the thoracic midline by Scer\GAL4^pnr-MD237 causes a hypo-pigmentation phenotype in the adult thorax and abdomen, compared to controls.

(Mercer and Burke, 2016)

Expression of ATP7^GD3322 driven by Scer\GAL4^elav-C155 does not significantly affect larval lethality compared to controls.

(Mercer et al., 2016)

Expression of ATP7^GD3322 under the control of Scer\GAL4^pnr-MD237 results in a strong hypopigmentation phenotype in the adult dorsal cuticle of the thorax and abdomen (in the domain of Scer\GAL4 expression).

Expression of ATP7^GD3322 under the control of Scer\GAL4^GMR.PFa has no effect on the eye.

(Binks et al., 2010)

Adults expressing ATP7^GD3322 under the control of Scer\GAL4^elav.PLu (in the presence of Dcr-2^Scer\UAS.cDa to increase the efficiency of RNAi) do not show a significant defect in avoidance of noxious temperature (46[o]C) compared to control flies.

(Neely et al., 2010)

Expression under the control of Scer\GAL4^pnr-MD237 results in a colour difference between the central Scer\GAL4^pnr-MD237 expression domain of the notum and the surrounding lateral region in 100% of the Scer\GAL4^pnr-MD237 expression domain.

Expression under the control of Scer\GAL4^pnr-MD237 results in bristle morphology defects on the notum in 100% of the Scer\GAL4^pnr-MD237 expression domain.

(Mummery-Widmer et al., 2009)

External Data

Linkouts

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development

3322

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT Enhanced by

Statement

Reference

ATP7^GD3322, Scer\GAL4^pnr-MD237 has abnormal body color phenotype, non-enhanceable by Grx1^GD10587, Scer\GAL4^pnr-MD237

(Mercer and Burke, 2016)

NOT suppressed by

Statement

Reference

ATP7^GD3322, Scer\GAL4^pnr-MD237 has abnormal body color phenotype, non-suppressible by Grx1^GD10587, Scer\GAL4^pnr-MD237

(Mercer and Burke, 2016)

Enhancer of

Statement

Reference

ATP7^GD3322, Scer\GAL4^GMR.PU is an enhancer of abnormal eye color phenotype of Hsap\HTT^Q93.ex1.UAS, Scer\GAL4^GMR.PU

(Xiao et al., 2013)

ATP7^GD3322, Scer\GAL4^elav.PU is an enhancer of partially lethal phenotype of Hsap\HTT^Q93.ex1.UAS, Scer\GAL4^elav.PU

(Xiao et al., 2013)

ATP7^GD3322, Scer\GAL4^elav.PU is an enhancer of abnormal locomotor behavior | adult stage phenotype of Hsap\HTT^Q93.ex1.UAS, Scer\GAL4^elav.PU

(Xiao et al., 2013)

ATP7^GD3322, Scer\GAL4^elav.PU is an enhancer of short lived phenotype of Hsap\HTT^Q93.ex1.UAS, Scer\GAL4^elav.PU

(Xiao et al., 2013)

NOT Enhancer of

Statement

Reference

ATP7^GD3322, Scer\GAL4^pnr-MD237 is a non-enhancer of visible phenotype of Grx1^GD10587, Scer\GAL4^pnr-MD237

(Mercer and Burke, 2016)

ATP7^GD3322, Scer\GAL4^elav-C155 is a non-enhancer of majority die during larval stage phenotype of Gclc^GD9767, Scer\GAL4^elav-C155

(Mercer et al., 2016)

ATP7^GD3322, Scer\GAL4^elav.PU is a non-enhancer of short lived | heat sensitive phenotype of Hsap\HTT^{M8V.H82A.Q127.ex1p.UAS}, Scer\GAL4^elav.PU

(Xiao et al., 2013)

ATP7^GD3322, Scer\GAL4^elav.PU is a non-enhancer of abnormal locomotor behavior | adult stage | heat sensitive phenotype of Hsap\HTT^{M8V.H82A.Q127.ex1p.UAS}, Scer\GAL4^elav.PU

(Xiao et al., 2013)

ATP7^GD3322, Scer\GAL4^GMR.PU is a non-enhancer of abnormal eye color | heat sensitive phenotype of Hsap\HTT^{M8V.H82A.Q127.ex1p.UAS}, Scer\GAL4^GMR.PU

(Xiao et al., 2013)

NOT Suppressor of

Statement

Reference

ATP7^GD3322, Scer\GAL4^pnr-MD237 is a non-suppressor of visible phenotype of Grx1^GD10587, Scer\GAL4^pnr-MD237

(Mercer and Burke, 2016)

ATP7^GD3322, Scer\GAL4^elav.PU is a non-suppressor of short lived | heat sensitive phenotype of Hsap\HTT^{M8V.H82A.Q127.ex1p.UAS}, Scer\GAL4^elav.PU

(Xiao et al., 2013)

ATP7^GD3322, Scer\GAL4^elav.PU is a non-suppressor of abnormal locomotor behavior | adult stage | heat sensitive phenotype of Hsap\HTT^{M8V.H82A.Q127.ex1p.UAS}, Scer\GAL4^elav.PU

(Xiao et al., 2013)

ATP7^GD3322, Scer\GAL4^GMR.PU is a non-suppressor of abnormal eye color | heat sensitive phenotype of Hsap\HTT^{M8V.H82A.Q127.ex1p.UAS}, Scer\GAL4^GMR.PU

(Xiao et al., 2013)

ATP7^GD3322, Scer\GAL4^GMR.PFa is a non-suppressor of visible phenotype of Ctr1A^GD16726, Scer\GAL4^GMR.PFa

(Binks et al., 2010)

Other

Statement

Reference

ATP7^GD3322, Ctr1B^UAS.Tag:FLAG, Scer\GAL4^GMR.PFa has lethal | male phenotype

(Binks et al., 2010)

ATP7^GD3322, Ctr1B^UAS.Tag:FLAG, Scer\GAL4^GMR.PFa has visible | female phenotype

(Binks et al., 2010)

ATP7^GD3322, Ctr1B^UAS.Tag:FLAG, Scer\GAL4^GMR.PFa has abnormal eye color | female phenotype

(Binks et al., 2010)

ATP7^GD3322, Ctr1A^{dN.UAS.Tag:FLAG}, Scer\GAL4^GMR.PFa has lethal | male phenotype

(Binks et al., 2010)

ATP7^GD3322, Ctr1A^{dN.UAS.Tag:FLAG}, Scer\GAL4^GMR.PFa has visible | female phenotype

(Binks et al., 2010)

ATP7^GD3322, Ctr1A^{dN.UAS.Tag:FLAG}, Scer\GAL4^GMR.PFa has abnormal eye color | female phenotype

(Binks et al., 2010)

Phenotype Manifest In

Enhancer of

Statement

Reference

ATP7^GD3322, Scer\GAL4^GMR.PU is an enhancer of eye phenotype of Hsap\HTT^Q93.ex1.UAS, Scer\GAL4^GMR.PU

(Xiao et al., 2013)

ATP7^GD3322, Scer\GAL4^GMR.PU is an enhancer of rhabdomere phenotype of Hsap\HTT^Q93.ex1.UAS, Scer\GAL4^GMR.PU

(Xiao et al., 2013)

NOT Enhancer of

Statement

Reference

ATP7^GD3322, Scer\GAL4^pnr-MD237 is a non-enhancer of adult thorax phenotype of Grx1^GD10587, Scer\GAL4^pnr-MD237

(Mercer and Burke, 2016)

ATP7^GD3322, Scer\GAL4^pnr-MD237 is a non-enhancer of scutellum phenotype of Grx1^GD10587, Scer\GAL4^pnr-MD237

(Mercer and Burke, 2016)

NOT Suppressor of

Statement

Reference

ATP7^GD3322, Scer\GAL4^pnr-MD237 is a non-suppressor of adult thorax phenotype of Grx1^GD10587, Scer\GAL4^pnr-MD237

(Mercer and Burke, 2016)

ATP7^GD3322, Scer\GAL4^pnr-MD237 is a non-suppressor of scutellum phenotype of Grx1^GD10587, Scer\GAL4^pnr-MD237

(Mercer and Burke, 2016)

ATP7^GD3322, Scer\GAL4^GMR.PFa is a non-suppressor of eye phenotype of Ctr1A^GD16726, Scer\GAL4^GMR.PFa

(Binks et al., 2010)

Other

Statement

Reference

ATP7^GD3322, Ctr1B^UAS.Tag:FLAG, Scer\GAL4^GMR.PFa has eye | female phenotype

(Binks et al., 2010)

ATP7^GD3322, Ctr1A^{dN.UAS.Tag:FLAG}, Scer\GAL4^GMR.PFa has eye | female phenotype

(Binks et al., 2010)

Additional Comments

Genetic Interactions

Statement

Reference

Co-expression of ATP7^GD3322 along with Grx1^GD10587 driven in the thoracic midline by Scer\GAL4^pnr-MD237 results in an additive phenotype, with both thoracic cleft and hypo-pigmentation visible in the adult.

(Mercer and Burke, 2016)

Co-expression of ATP7^GD3322 does not significantly enhance larval lethality seen with expression of Gclc^GD9767 driven by Scer\GAL4^elav-C155.

(Mercer et al., 2016)

Co-expression of Phm^KK112513 and ATP7^GD3322 under the control of Scer\GAL4^elav-C155 does not result in any obvious phenotype, as compared to controls.

(Hwang et al., 2014)

Co-expression of ATP7^GD3322 has no effect on the eye phenotype caused by expression of Ctr1A^GD16726 under the control of Scer\GAL4^GMR.PFa.

Co-expression of Ctr1B^{Scer\UAS.T:Zzzz\FLAG} and ATP7^GD3322 under the control of Scer\GAL4^GMR.PFa results in male lethality, while females have a severe rough eye phenotype together with loss of eye pigmentation.

Co-expression of Ctr1A^{Scer\UAS.T:Zzzz\FLAG} and ATP7^GD3322 under the control of Scer\GAL4^GMR.PFa results in male lethality, while females have a severe rough eye phenotype.

(Binks et al., 2010)

Xenogenetic Interactions

Statement

Reference

Co-expression of ATP7^GD3322 enhances the mobility defects of flies expressing Hsap\HTT^{Q93.ex1p.Scer\UAS} under the control of Scer\GAL4^elav.PU.

Co-expression of ATP7^GD3322 enhances loss of eye pigmentation and progressive loss of rhabdomeres seen in flies expressing Hsap\HTT^{Q93.ex1p.Scer\UAS} under the control of Scer\GAL4^GMR.PU.

Co-expression of ATP7^GD3322 enhances the aggregation of Hsap\HTT^{Q93.ex1p.Scer\UAS} protein seen in the brains of flies expressing Hsap\HTT^{Q93.ex1p.Scer\UAS} under the control of Scer\GAL4^elav.PU.

Co-expression of ATP7^GD3322 has no effect on the reduction in lifespan seen in flies expressing Hsap\HTT^{M8V.H82A.Q127.ex1p.Scer\UAS} under the control of Scer\GAL4^elav.PU at 29[o]C.

Co-expression of ATP7^GD3322 has no effect on the mobility defects seen in flies expressing Hsap\HTT^{M8V.H82A.Q127.ex1p.Scer\UAS} under the control of Scer\GAL4^elav.PU at 29[o]C.

Co-expression of ATP7^GD3322 has no effect on the loss of eye pigmentation seen in flies expressing Hsap\HTT^{M8V.H82A.Q127.ex1p.Scer\UAS} under the control of Scer\GAL4^GMR.PU at 29[o]C.

(Xiao et al., 2013)

Complementation and Rescue Data

Rescued by

ATP7^GD3322 is rescued by Scer\GAL4^pnr-MD237/ATP7^UAS.Tag:FLAG

(Binks et al., 2010)

Comments

Co-expression of ATP7^{Scer\UAS.T:Zzzz\FLAG} rescues the cuticle hypopigmentation phenotype caused by expression of ATP7^GD3322 under the control of Scer\GAL4^pnr-MD237.

(Binks et al., 2010)

Images (0)

Mutant

Wild-type

Stocks (1)

VDRC

v8315

w¹¹¹⁸; P{GD3322}v8315

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 1 )

Linkouts