FlyBase Allele Report: Dmel\Gclc[GD9767]

General Information

Symbol

Dmel\Gclc^GD9767

Species

D. melanogaster

Name

FlyBase ID

FBal0207100

Feature type

allele

Associated gene

Dmel\Gclc

Associated Insertion(s)

Carried in Construct

P{GD9767}

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

(Dickson et al., 2007.7.18)

Progenitor genotype

Carried in construct

P{GD9767}

Cytology

Description

UASt regulatory sequences drive expression of an inverted repeat.

(Dickson et al., 2007.7.18)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

dsRNA-GD9767

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neuroanatomy | third instar larval stage, with Scer\GAL4^CCAP.PP

(Mercer et al., 2016)

decreased cell size | third instar larval stage, with Scer\GAL4^CCAP.PP

(Mercer et al., 2016)

lethal, with Scer\GAL4^Act.PU

(Mercer et al., 2016)

lethal, with Scer\GAL4^Tub.PU

(Mercer et al., 2016)

majority die during larval stage, with Scer\GAL4^elav-C155

(Mercer et al., 2016)

partially lethal - majority die, with Scer\GAL4^elav-C155

(Mercer et al., 2016)

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

(Neely et al., 2010)

viable, with Scer\GAL4^Mef2.PR

(Schnorrer et al., 2010)

viable, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

viable, with Scer\GAL4^tin.CΔ4

(Neely et al., 2010)

visible, with Scer\GAL4^CCAP.PP

(Mercer et al., 2016)

visible, with Scer\GAL4^elav-C155

(Mercer et al., 2016)

Phenotype Manifest In

adult thorax, with Scer\GAL4^CCAP.PP

(Mercer et al., 2016)

adult thorax, with Scer\GAL4^elav-C155

(Mercer et al., 2016)

axon | third instar larval stage, with Scer\GAL4^CCAP.PP

(Mercer et al., 2016)

embryonic/larval brain | third instar larval stage, with Scer\GAL4^CCAP.PP

(Mercer et al., 2016)

wing, with Scer\GAL4^CCAP.PP

(Mercer et al., 2016)

wing, with Scer\GAL4^elav-C155

(Mercer et al., 2016)

Detailed Description

Statement

Reference

No obvious phenotypes are seen when expression of Gclc^GD9767 is driven by Scer\GAL4^GMR.PFa (adult eye) or Scer\GAL4^pnr-MD237 (dorsal thorax).

Expression of Gclc^GD9767 driven by Scer\GAL4^elav-C155 leads to developmental arrest at the pupal stage or earlier, with only 19% of flies reaching pupation; rare adult survivors display a sunken thorax with a cleft and unexpanded wings. Larval survival at 96hr post egg hatching is about 56% of that seen in controls when expression of Gclc^GD9767 is driven by Scer\GAL4^elav-C155.

Expression of Gclc^GD9767 driven by Scer\GAL4^CCAP.PP leads to a fully penetrant unexpanded wing phenotype, along with a softened and clefted thoracic cuticle. Scer\GAL4^CCAP.PP>Gclc^GD9767 third instar larval brains show a significant decrease in the density of axon branching compared to controls; though the number of Scer\GAL4^CCAP.PP neurons is unchanged, their area is significantly smaller than controls.

(Mercer et al., 2016)

Adults expressing Gclc^GD9767 under the control of Scer\GAL4^elav.PLu (in the presence of Dcr-2^Scer\UAS.cDa to increase the efficiency of RNAi) do not show a significant defect in avoidance of noxious temperature (46[o]C) compared to control flies.

(Neely et al., 2010)

External Data

Linkouts

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development

9767

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Gclc^GD9767, Scer\GAL4^elav-C155 has majority die during larval stage phenotype, enhanceable by ATP7^UAS.Tag:FLAG, Scer\GAL4^elav-C155

(Mercer et al., 2016)

Gclc^GD9767, Scer\GAL4^elav-C155 has majority die during larval stage phenotype, enhanceable by Ctr1A^GD16726, Scer\GAL4^elav-C155

(Mercer et al., 2016)

Gclc^GD9767, Scer\GAL4^CCAP.PP has abnormal neuroanatomy | third instar larval stage phenotype, enhanceable by ATP7^UAS.Tag:FLAG, Scer\GAL4^CCAP.PP

(Mercer et al., 2016)

Gclc^GD9767, Scer\GAL4^CCAP.PP has decreased cell size | third instar larval stage phenotype, enhanceable by ATP7^UAS.Tag:FLAG, Scer\GAL4^CCAP.PP

(Mercer et al., 2016)

NOT Enhanced by

Statement

Reference

Gclc^GD9767, Scer\GAL4^elav-C155 has majority die during larval stage phenotype, non-enhanceable by ATP7^GD3322, Scer\GAL4^elav-C155

(Mercer et al., 2016)

Gclc^GD9767, Scer\GAL4^elav-C155 has majority die during larval stage phenotype, non-enhanceable by Ctr1B^GD2859, Scer\GAL4^elav-C155

(Mercer et al., 2016)

Phenotype Manifest In

Enhanced by

Statement

Reference

Gclc^GD9767, Scer\GAL4^CCAP.PP has embryonic/larval brain | third instar larval stage phenotype, enhanceable by ATP7^UAS.Tag:FLAG, Scer\GAL4^CCAP.PP

(Mercer et al., 2016)

Gclc^GD9767, Scer\GAL4^CCAP.PP has axon | third instar larval stage phenotype, enhanceable by ATP7^UAS.Tag:FLAG, Scer\GAL4^CCAP.PP

(Mercer et al., 2016)

Additional Comments

Genetic Interactions

Statement

Reference

Co-expression of ATP7^{Scer\UAS.T:Zzzz\FLAG} or Ctr1A^GD16726 significantly enhances larval lethality seen with expression of Gclc^GD9767 driven by Scer\GAL4^elav-C155. Co-expression of ATP7^GD3322 or Ctr1B^GD2859 does not significantly enhance larval lethality seen with expression of Gclc^GD9767 driven by Scer\GAL4^elav-C155. Co-expression of ATP7^{Scer\UAS.T:Zzzz\FLAG} significantly exacerbates the decrease of axon branching and Scer\GAL4^CCAP.PP neuron area in third instar larval brains with expression of Gclc^GD9767 driven by Scer\GAL4^CCAP.PP.

(Mercer et al., 2016)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

VDRC

v33512

w¹¹¹⁸; P{GD9767}v33512

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 1 )

Linkouts

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development

9767

Synonyms and Secondary IDs (1)

Reported As

Symbol Synonym

Gclc^GD9767

Name Synonyms

Secondary FlyBase IDs

References (8)

Report Sections

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