FlyBase Allele Report: Dmel\Eip75B[Δ51]

General Information

Symbol

Dmel\Eip75B^Δ51

Species

D. melanogaster

Name

FlyBase ID

FBal0147922

Feature type

allele

Associated gene

Dmel\Eip75B

Associated Insertion(s)

Carried in Construct

Also Known As

E75^Δ51

Key Links

Allele class

Mutagen

P-element activity

Nature of the Allele

Allele class

Mutagen

P-element activity

(Bialecki et al., 2002)

Progenitor genotype

Eip75B⁰³³³⁸

(Bialecki et al., 2002)

Cytology

Description

Imprecise excision of P{PZ}Eip75B⁰³³³⁸ has caused a deletion removing approximately 30 kb of DNA, taking out the 4th and 5th exons of Eip75B (exons shared by all 3 isoforms (A, B & C).

(Bialecki et al., 2002)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Disease

Evidence

References

model of amyotrophic lateral sclerosis

CEA with Hsap\TARDBP^UAS.YFP.cEa

(Joardar et al., 2015)

CEA with Hsap\TARDBP^{G298S.UAS.YFP}

(Joardar et al., 2015)

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal developmental rate (with Eip75B^A81)

(Bialecki et al., 2002)

abnormal developmental rate | larval stage | recessive

(Bialecki et al., 2002)

abnormal neuroanatomy | somatic clone

(Rabinovich et al., 2016)

fertile (with Eip75B^Δ1)

(Bialecki et al., 2002)

flightless (with Eip75B^x37)

(Bialecki et al., 2002)

lethal | pharate adult stage (with Eip75B^A81)

(Bialecki et al., 2002)

lethal - all die before end of larval stage | recessive

(Bialecki et al., 2002)

lethal - all die before end of second instar larval stage (with Eip75B^A81)

(Chen et al., 2008)

partially lethal - majority die (with Eip75B^x37)

(Bialecki et al., 2002)

partially lethal - majority die | larval stage (with Eip75B^A81)

(Bialecki et al., 2002)

partially lethal - majority die | prepupal stage (with Eip75B^A81)

(Bialecki et al., 2002)

partially lethal - majority live | adult stage (with Eip75B^x37)

(Bialecki et al., 2002)

some die during larval stage | recessive

(Bialecki et al., 2002)

some die during pharate adult stage (with Eip75B^x37)

(Bialecki et al., 2002)

uncoordinated (with Eip75B^x37)

(Bialecki et al., 2002)

viable (with Eip75B^Δ1)

(Bialecki et al., 2002)

Phenotype Manifest In

adult mushroom body gamma-lobe | adult stage | somatic clone

(Rabinovich et al., 2016)

embryonic/larval anterior spiracle (with Eip75B^A81)

(Bialecki et al., 2002)

eye (with Eip75B^x37)

(Bialecki et al., 2002)

mouth hook (with Eip75B^A81)

(Bialecki et al., 2002)

Detailed Description

Statement

Reference

Eip75B^Δ51/Eip75B^Δ51 mutant mushroom body neuron clones fail to fully innervate the adult gamma lobe during regrowth at metamorphosis, and also display a weak pruning defect, but there is no defect in initial extension of these neurons, as compared to wild type.

(Rabinovich et al., 2016)

Almost all Eip75B^A81/Eip75B^Δ51 mutant second instar larvae fail to molt and develop to later stages. Feeding ecdysone to these second instar larvae rescues 50% of them to the pupal stage.

(Chen et al., 2008)

Eip75B^Δ51 homozygotes remain as first instar larvae for over a week before dying without any detectable morphological abnormalities. Approximately 33% of Eip75B¹/Eip75B^Δ51 animals die as pharate adults, with normal adult pigmentation and fully developed appendages. The animals eclose and are severely uncoordinated, displaying difficulty in walking and an inability to fly. These animals die within a week following eclosion, but appear morphologically normal with the exception of black spots that cover about one quarter of the surface of the eye. Eip75B^Δ1/Eip75B^Δ51 animals are viable and fertile, with no detectable phenotypes. Subsets of first and second instar Eip75B^A81/Eip75B^Δ51 larvae never molt to the next instar, surviving for up to a week before dying. Those that molt do so up to 12 hr late, while some die at the molt. These animals often have malformed mouthhooks or two sets of mouthhooks. Among the remainder, significant lethality occurs in the 3rd instar, and pre-pupal stages, with the rest dying as pharate adults that, while failing to eclose, have no obvious morphological defects. Approximately 20% of Eip75B^A81/Eip75B^Δ51 second instar larvae fail to undergo the 2nd to 3rd instar molt but nevertheless pupariate, despite maintaining 2nd instar morphology as judged by mouthhooks and anterior spiracles. This pupariation takes place approximately 88 hours after the 1st to 2nd instar molt. About 20% of the L2 prepupae (n = 85) develop to the pupal stage as evidenced by head eversion and leg and wing extension. Eip75B^A81/Eip75B^Δ51 second instar larvae have a reduced ecdysteroid titer, and their molting defects can be rescued by treatment with ecdysteroids.

(Bialecki et al., 2002)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT Enhanced by

Statement

Reference

Eip75B^Δ51 has abnormal neuroanatomy | somatic clone phenotype, non-enhanceable by Hr51^LL04325/Hr51^LL04325

(Rabinovich et al., 2016)

Suppressed by

Statement

Reference

Eip75B^Δ51 has abnormal neuroanatomy | somatic clone phenotype, suppressible | partially by Scer\GAL4^Tab2-201Y/Rheb^UAS.cUa

(Rabinovich et al., 2016)

Eip75B^Δ51 has abnormal neuroanatomy | somatic clone phenotype, suppressible | partially by S6k^STDE.UAS/Scer\GAL4^Tab2-201Y

(Rabinovich et al., 2016)

NOT suppressed by

Statement

Reference

Eip75B^Δ51 has abnormal neuroanatomy | somatic clone phenotype, non-suppressible by Scer\GAL4^Tab2-201Y/Hr51^UAS.Tag:FLAG

(Rabinovich et al., 2016)

NOT Enhancer of

Statement

Reference

Eip75B^Δ51/Eip75B^Δ51 is a non-enhancer of abnormal neuroanatomy | somatic clone phenotype of Hr51^LL04325

(Rabinovich et al., 2016)

Other

Statement

Reference

Eip75B^Δ51/Eip75B[+], Hsap\TARDBP^UAS.YFP.cEa, Scer\GAL4^Toll-6-D42 has abnormal locomotor behavior | third instar larval stage phenotype

(Joardar et al., 2015)

Eip75B^Δ51/Eip75B[+], Hsap\TARDBP^{G298S.UAS.YFP}, Scer\GAL4^Toll-6-D42 has abnormal locomotor behavior | third instar larval stage phenotype

(Joardar et al., 2015)

Phenotype Manifest In

NOT Enhanced by

Statement

Reference

Eip75B^Δ51 has adult mushroom body gamma-lobe | adult stage | somatic clone phenotype, non-enhanceable by Hr51^LL04325/Hr51^LL04325

(Rabinovich et al., 2016)

Suppressed by

Statement

Reference

Eip75B^Δ51 has adult mushroom body gamma-lobe | somatic clone | adult stage phenotype, suppressible | partially by Scer\GAL4^Tab2-201Y/Rheb^UAS.cUa

(Rabinovich et al., 2016)

Eip75B^Δ51 has adult mushroom body gamma-lobe | somatic clone | adult stage phenotype, suppressible | partially by S6k^STDE.UAS/Scer\GAL4^Tab2-201Y

(Rabinovich et al., 2016)

NOT suppressed by

Statement

Reference

Eip75B^Δ51 has adult mushroom body gamma-lobe | somatic clone | adult stage phenotype, non-suppressible by Scer\GAL4^Tab2-201Y/Hr51^UAS.Tag:FLAG

(Rabinovich et al., 2016)

NOT Enhancer of

Statement

Reference

Eip75B^Δ51/Eip75B^Δ51 is a non-enhancer of adult mushroom body gamma-lobe | adult stage | somatic clone phenotype of Hr51^LL04325

(Rabinovich et al., 2016)

Additional Comments

Genetic Interactions

Statement

Reference

Expression of S6k^{STDE.Scer\UAS} or Rheb^Scer\UAS.cUa under the control of Scer\GAL4^Tab2-201Y partially rescues the gamma lobe regrowth defects of Eip75B^Δ51/Eip75B^Δ51 mushroom body neuron clones.

Eip75B^Δ51/Eip75B^Δ51, Hr51^LL04325/Hr51^LL04325 double mutant mushroom body neuron clones do not show a significantly more severe regrowth defect than either single mutant.

Expression of Hr51^{Scer\UAS.T:Zzzz\FLAG} under the control of Scer\GAL4^Tab2-201Y fails to suppress the gamma lobe regrowth defects of Eip75B^Δ51/Eip75B^Δ51 mushroom body neuron clones.

(Rabinovich et al., 2016)

The levels of follicle death (scored as percentage of ovarioles containing at least one dying follicle) in Eip78C^Δ31;Eip75B^Δ51 double heterozygote females is not significantly increased compared to either Eip78C^Δ31/+ or Eip75B^Δ51/+ alone.

(Ables et al., 2015)

Xenogenetic Interactions

Statement

Reference

Pioglitazone can no longer rescue the Scer\GAL4^D42>Hsap\TARDBP^{Scer\UAS.T:Avic\GFP-YFP.cEa}-dependent larval turning defects in a heterozygous Eip75B^Δ51 genetic background.

Pioglitazone can no longer rescue the Scer\GAL4^D42>Hsap\TARDBP^{G298S.Scer\UAS.T:Avic\GFP-YFP}-dependent larval turning defects in a heterozygous Eip75B^Δ51 genetic background.

(Joardar et al., 2015)

Complementation and Rescue Data

Rescued by

Eip75B^Δ51 is rescued by Scer\GAL4^Tab2-201Y/Eip75B^{UAS.A.Tag:FLAG}

(Rabinovich et al., 2016)

Eip75B^Δ51 is rescued by Eip75B^{UAS.B.Tag:FLAG}/Scer\GAL4^Tab2-201Y

(Rabinovich et al., 2016)

Eip75B^Δ51 is rescued by Eip75B^{UAS.C.Tag:FLAG}/Scer\GAL4^Tab2-201Y

(Rabinovich et al., 2016)

Comments

Expression of Eip75B^{Scer\UAS.A.T:Zzzz\FLAG}, Eip75B^{Scer\UAS.B.T:Zzzz\FLAG}, or Eip75B^{Scer\UAS.C.T:Zzzz\FLAG} under the control of Scer\GAL4^Tab2-201Y significantly rescues the gamma lobe regrowth defects of Eip75B^Δ51/Eip75B^Δ51 mushroom body neuron clones.

(Rabinovich et al., 2016)