FlyBase Allele Report: Dmel\Hr51[LL04325]

General Information

Symbol

Dmel\Hr51^LL04325

Species

D. melanogaster

Name

FlyBase ID

FBal0279818

Feature type

allele

Associated gene

Dmel\Hr51

Associated Insertion(s)

PBac{SAstopDsRed}LL04325

Carried in Construct

Also Known As

unf^LL04325

Key Links

Allele class

Mutagen

piggyBac activity

Nature of the Allele

Allele class

Mutagen

piggyBac activity

(Yaniv et al., 2012)

Progenitor genotype

Associated Insertion(s)

PBac{SAstopDsRed}LL04325

Cytology

Description

PBac{SAstopDsRed}LL04325 is inserted into the first intron of Hr51.

(Yaniv et al., 2012)

Allele components

Component

Use(s)

Inserted element

PBac{SAstopDsRed}

gene trap

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neuroanatomy | somatic clone

(Rabinovich et al., 2016, Yaniv et al., 2012)

lethal

(Yaniv et al., 2012)

Phenotype Manifest In

adult mushroom body gamma-lobe | adult stage | somatic clone

(Rabinovich et al., 2016)

gamma Kenyon cell | somatic clone

(Yaniv et al., 2012)

Detailed Description

Statement

Reference

Hr51^LL04325/Hr51^LL04325 mutant mushroom body neuron clones fail to fully innervate the adult gamma lobe during regrowth at metamorphosis.

(Rabinovich et al., 2016)

Hr51^LL04325 MARCM neuroblast clones exhibit aberrant adult mushroom body γ lobes. γ-neuron axons homozygous for Hr51^LL04325 do not extend and elaborate into the adult-specific lobe.

Hr51^LL04325 single cell γ-neuron clones display poor innervation of the γ-lobe.

Mutant Hr51^LL04325 γ-neuron clones appear normal in third-instar larvae (L3), and axon-pruning occurs with similar dynamics and to the same extent as in controls. However, in contrast to controls, mutant axons fail to initiate axon sprouting at 24 hours after puparium formation. Non-clonal surrounding neurons, however, initiate axon growth normally. There are no signs of degeneration, such as blebbing or degenerated axons through development or in adult mutant clones. α/β neurons exhibit normal morphology and show no signs of axon stalling.

(Yaniv et al., 2012)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT Enhanced by

Statement

Reference

Hr51^LL04325 has abnormal neuroanatomy | somatic clone phenotype, non-enhanceable by Eip75B^Δ51/Eip75B^Δ51

(Rabinovich et al., 2016)

Suppressed by

Statement

Reference

Hr51^LL04325 has abnormal neuroanatomy | somatic clone phenotype, suppressible | partially by Tsc1²⁹

(Yaniv et al., 2012)

Hr51^LL04325 has abnormal neuroanatomy | somatic clone phenotype, suppressible | partially by S6k^STDE.UAS/Scer\GAL4^Tab2-201Y

(Yaniv et al., 2012)

Hr51^LL04325 has abnormal neuroanatomy | somatic clone phenotype, suppressible | partially by Rheb^UAS.cPa/Scer\GAL4^Tab2-201Y

(Yaniv et al., 2012)

NOT suppressed by

Statement

Reference

Hr51^LL04325 has abnormal neuroanatomy | somatic clone phenotype, non-suppressible by Scer\GAL4^Tab2-201Y/Eip75B^{UAS.A.Tag:FLAG}

(Rabinovich et al., 2016)

Hr51^LL04325 has abnormal neuroanatomy | somatic clone phenotype, non-suppressible by Eip75B^{UAS.B.Tag:FLAG}/Scer\GAL4^Tab2-201Y

(Rabinovich et al., 2016)

NOT Enhancer of

Statement

Reference

Hr51^LL04325/Hr51^LL04325 is a non-enhancer of abnormal neuroanatomy | somatic clone phenotype of Eip75B^Δ51

(Rabinovich et al., 2016)

Phenotype Manifest In

NOT Enhanced by

Statement

Reference

Hr51^LL04325 has adult mushroom body gamma-lobe | adult stage | somatic clone phenotype, non-enhanceable by Eip75B^Δ51/Eip75B^Δ51

(Rabinovich et al., 2016)

Suppressed by

Statement

Reference

Hr51^LL04325 has gamma Kenyon cell | somatic clone phenotype, suppressible | partially by Tsc1²⁹

(Yaniv et al., 2012)

Hr51^LL04325 has gamma Kenyon cell | somatic clone phenotype, suppressible | partially by S6k^STDE.UAS/Scer\GAL4^Tab2-201Y

(Yaniv et al., 2012)

Hr51^LL04325 has gamma Kenyon cell | somatic clone phenotype, suppressible | partially by Rheb^UAS.cPa/Scer\GAL4^Tab2-201Y

(Yaniv et al., 2012)

NOT suppressed by

Statement

Reference

Hr51^LL04325 has adult mushroom body gamma-lobe | somatic clone | adult stage phenotype, non-suppressible by Scer\GAL4^Tab2-201Y/Eip75B^{UAS.A.Tag:FLAG}

(Rabinovich et al., 2016)

Hr51^LL04325 has adult mushroom body gamma-lobe | somatic clone | adult stage phenotype, non-suppressible by Eip75B^{UAS.B.Tag:FLAG}/Scer\GAL4^Tab2-201Y

(Rabinovich et al., 2016)

NOT Enhancer of

Statement

Reference

Hr51^LL04325/Hr51^LL04325 is a non-enhancer of adult mushroom body gamma-lobe | adult stage | somatic clone phenotype of Eip75B^Δ51

(Rabinovich et al., 2016)

Other

Statement

Reference

Hr51^LL04325, S6k^STDE.UAS, Scer\GAL4^Tab2-201Y has alpha/beta Kenyon cell | somatic clone phenotype

(Yaniv et al., 2012)

Hr51^LL04325, Rheb^UAS.cPa, Scer\GAL4^Tab2-201Y has alpha/beta Kenyon cell | somatic clone phenotype

(Yaniv et al., 2012)

Additional Comments

Genetic Interactions

Statement

Reference

Eip75B^Δ51/Eip75B^Δ51, Hr51^LL04325/Hr51^LL04325 double mutant mushroom body neuron clones do not show a significantly more severe regrowth defect than either single mutant.

Expression of Eip75B^{Scer\UAS.A.T:Zzzz\FLAG} or Eip75B^{Scer\UAS.B.T:Zzzz\FLAG} under the control of Scer\GAL4^Tab2-201Y fails to rescue the gamma lobe regrowth defects of Hr51^LL04325/Hr51^LL04325 mushroom body neuron clones.

(Rabinovich et al., 2016)

The developmental regrowth defect seen in Hr51^LL04325 MARCM neuroblast clones is partially suppressed in clones that are doubly mutant for Hr51^LL04325 and Tsc1²⁹.

Expression of S6k^{STDE.Scer\UAS} in Hr51^LL04325 MARCM neuroblast clones partially suppresses the developmental regrowth defect. In these clones, γ axons do not occupy the entire adult lobe but instead grow to the dorsal and ventral extremities of the adult γ-lobe and in some cases only to its ventral portion.

Expression of Rheb^Scer\UAS.cPa in Hr51^LL04325 MARCM neuroblast clones partially suppresses the developmental regrowth defect. In addition these double mutants exhibit severe axon guidance defects. In these clones, γ axons do not occupy the entire adult lobe but instead grow to the dorsal and ventral extremities of the adult γ-lobe and in some cases only to its ventral portion.

(Yaniv et al., 2012)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

Hr51^LL04325 is rescued by Hr51^cSa

(Yaniv et al., 2012)

Hr51^LL04325 is rescued by Scer\GAL4^Tab2-201Y/Hr51^UAS.Tag:FLAG

(Yaniv et al., 2012)

Hr51^LL04325 is rescued by Hr51^{tll.P-box.UAS.Tag:FLAG}/Scer\GAL4^Tab2-201Y

(Yaniv et al., 2012)