FlyBase Allele Report: Dmel\pygo[S123]

General Information

Symbol

Dmel\pygo^S123

Species

D. melanogaster

Name

FlyBase ID

FBal0146872

Feature type

allele

Associated gene

Dmel\pygo

Associated Insertion(s)

Carried in Construct

Key Links

Allele class

loss of function allele

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

loss of function allele

(Townsley et al., 2004, Thompson et al., 2002)

Mutagen

ethyl methanesulfonate

(Thompson et al., 2002)

Progenitor genotype

Cytology

Description

Small deletion close to the pygo translation initiation site, resulting in a truncation that retains only 51 amino acids.

(Thompson et al., 2002)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

Detailed Description

Statement

Reference

Adulthood-generated pygo^S123 homozygous somatic clones in mosaic midguts have a significant increase in cell number, as compared to adulthood-generated control somatic clones in wild-type midguts.

Generating pygo^S123 homozygous somatic clones in mosaic midguts during adulthood leads to an increase in the numbers of neighboring control intestinal progenitor cells, heterozygous for pygo^S123.

(Wang et al., 2016)

Haltere disc bearing clones mutant for pygo^S123 show severe patterning defects due to clone overgrowth.

Embryos from mothers with pygo^S123 mutant germline clones show a lawn of ventral denticles (a hallmark of wg signaling failure).

(Fiedler et al., 2015)

pygo^S123 clones 42 hours after pupal formation have abnormal cone cell morphology compared to wild type but a normal cone cell number. Cell death levels (as visualised by Caspase-3 staining) are similar to controls.

Clones induced in the eye discs of pygo^S123 third instar larvae display mild cone cell defects.

(Cordero and Cagan, 2010)

Homozygous embryos derived from homozygous female germ-line clones (lacking both maternal and zygotic pygo function) show a lawn of denticles phenotype). The regions of naked cuticle are largely restored in paternally rescued homozygous embryos (lacking only zygotic pygo function).

(Townsley et al., 2004)

pygo^S123 mutant embryos that lack maternal and zygotic pygo function display a cuticle denticle lawn that is characteristic of a complete loss of wg signalling.

(Townsley et al., 2004)

Mutant embryos derived from homozygous female germline clones (lacking both maternal and zygotic pygo function) are short and lack the naked stretches that are normally interspersed between the ventral denticle belts ("lawn of denticles" phenotype). The middle gut constriction is missing.

(Thompson et al., 2002)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressor of

Statement

Reference

pygo[+]/pygo^S123 is a suppressor of visible phenotype of arm^S56F.GMR

(van Tienen et al., 2017)

pygo[+]/pygo^S123 is a suppressor of visible phenotype of Chi^e5.5

(van Tienen et al., 2017)

pygo[+]/pygo^S123 is a suppressor | partially of visible phenotype of N^GD144, Scer\GAL4^sev.PU

(Cordero and Cagan, 2010)

pygo[+]/pygo^S123 is a suppressor | partially of decreased cell number | third instar larval stage phenotype of N^GD144, Scer\GAL4^sev.PU

(Cordero and Cagan, 2010)

Phenotype Manifest In

Suppressed by

Statement

Reference

pygo^S123 has embryonic/first instar larval cuticle | maternal effect phenotype, suppressible by Scer\GAL4^da.G32/arm^{UAS.Tag:MYC,Tag:NLS(SV40-largeT)}

(Townsley et al., 2004)

NOT suppressed by

Statement

Reference

pygo^S123 has embryonic/first instar larval cuticle | maternal effect phenotype, non-suppressible by arm^{ΔN.UAS.Tag:HA,Tag:Myr(Unk)}/Scer\GAL4^da.G32

(Townsley et al., 2004)

pygo^S123 has embryonic/first instar larval cuticle | germline clone phenotype, non-suppressible by arm^{S10.UAS.Tag:MYC}/Scer\GAL4^arm.PS

(Thompson et al., 2002)

Suppressor of

Statement

Reference

pygo[+]/pygo^S123 is a suppressor of wing phenotype of Chi^e5.5

(van Tienen et al., 2017)

pygo[+]/pygo^S123 is a suppressor of eye phenotype of arm^S56F.GMR

(van Tienen et al., 2017)

pygo[+]/pygo^S123 is a suppressor | partially of cone cell phenotype of N^GD144, Scer\GAL4^sev.PU

(Cordero and Cagan, 2010)

pygo[+]/pygo^S123 is a suppressor | partially of eye phenotype of N^GD144, Scer\GAL4^sev.PU

(Cordero and Cagan, 2010)

pygo[+]/pygo^S123 is a suppressor of phenotype of arm^S56F.GMR

(Thompson et al., 2002)

Additional Comments

Genetic Interactions

Statement

Reference

The wing notch phenotype seen in Chi^e5.5/+ mutants is partially suppressed by the presence of pygo^S123/+.

pygo^S123/+ suppresses the rough eye phenotype seen in flies expressing arm^S56F.GMR.

(van Tienen et al., 2017)

pygo^S123/+ suppresses the necrosis seen in adult eyes when N^GD144 is driven by Scer\GAL4^sev.PU.

pygo^S123/+ suppresses the cone cell loss seen in the third instar eye discs when N^GD144 is driven by Scer\GAL4^sev.PU.

(Cordero and Cagan, 2010)

Overexpression of arm^{Scer\UAS.T:Hsap\MYC,T:SV40\nls2}, under the regulation of Scer\GAL4^da.G32 suppresses the mutant phenotype of pygo^S123 embryos which lack both maternal and zygotic pygo function. The degree of suppression varies very little between individual embryos, and is essentially the same as that provided by zygotic pygo expressed from the paternal wild-type allele.

(Townsley et al., 2004)

Expression of arm^{S10.Scer\UAS.T:Hsap\MYC} under the control of Scer\GAL4^arm.PS has no effect on the cuticle phenotype of pygo^S123 embryos derived from pygo^S123 germline clones.

(Thompson et al., 2002)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Fails to complement

pygo^S28

(Cook, 2006.1.31)

Rescued by

pygo^S123/pygo¹³⁰ is rescued by pygo^Tag:HA

(Kessler et al., 2009)

pygo^S123/pygo¹³⁰ is rescued by pygo^Y748A.Tag:HA

(Kessler et al., 2009)

pygo^S123/pygo¹³⁰ is rescued by pygo^A763E.Tag:HA

(Kessler et al., 2009)

pygo^S123/pygo¹³⁰ is rescued by pygo^{Y748A.A763E.Tag:HA}

(Kessler et al., 2009)

pygo^S123/pygo¹³⁰ is rescued by pygo^{Y748A.F773E.Tag:HA}

(Kessler et al., 2009)

pygo^S123/pygo¹³⁰ is rescued by pygo^+tHa

(Staedeli and Basler, 2005)

pygo^S123 is rescued by pygo^UAS.Tag:HA/Scer\GAL4^da.G32

(Townsley et al., 2004)

pygo^S123 is rescued by Scer\GAL4^da.G32/pygo^{Nnpf.UAS.Tag:HA}

(Townsley et al., 2004)

pygo^S123 is rescued by Scer\GAL4^da.G32/pygo^{mLoop1.UAS.Tag:HA}

(Townsley et al., 2004)

Not rescued by

pygo^S123/pygo¹³⁰ is not rescued by pygo^V757E.Tag:HA

(Kessler et al., 2009)

pygo^S123/pygo¹³⁰ is not rescued by pygo^L789A.Tag:HA

(Kessler et al., 2009)

pygo^S123/pygo¹³⁰ is not rescued by pygo^F78A

(Staedeli and Basler, 2005)

pygo^S123 is not rescued by pygo^{mLoop2.UAS.Tag:HA}/Scer\GAL4^da.G32

(Townsley et al., 2004)

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

7209

w^*; P{neoFRT}82B pygo^S123/TM6B, P{Ubi-GFP.S65T}PAD2, Tb¹

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

S123

(Thompson et al., 2002)

pygo¹²³

(Staedeli and Basler, 2005)

pygo^S123.3

(Kessler et al., 2009)

pygo^S123

(Wang et al., 2019, van Tienen et al., 2017, Jenny and Basler, 2016, Tian et al., 2016, Wang et al., 2016, Fiedler et al., 2015, Luo et al., 2015, Miller et al., 2013, Cordero and Cagan, 2010, Jordan et al., 2006)

Name Synonyms

Secondary FlyBase IDs

References (16)

Report Sections

Open Close