FlyBase Allele Report: Dmel\pygo[S28]

General Information

Symbol

Dmel\pygo^S28

Species

D. melanogaster

Name

FlyBase ID

FBal0146871

Feature type

allele

Associated gene

Dmel\pygo

Associated Insertion(s)

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

loss of function allele

hypomorphic allele - genetic evidence

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

hypomorphic allele - genetic evidence

(Townsley et al., 2004)

loss of function allele

(Thompson et al., 2002)

Mutagen

ethyl methanesulfonate

(Thompson et al., 2002)

Progenitor genotype

Cytology

Description

Amino acid replacement: ?740term.

(Thompson et al., 2002)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

3R:31,577,413..31,577,413 [-]

Nucleotide change:

C31577413T

Amino acid change:

Q740term | pygo-PA

Reported amino acid change:

?740term

Comment:

Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change.

(Thompson et al., 2002)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal cell growth | somatic clone

(Giraldez and Cohen, 2003)

increased cell death | somatic clone

(Giraldez and Cohen, 2003)

lethal (with pygo^S123)

(Cook, 2006.1.31)

visible | somatic clone

(Thompson et al., 2002)

Phenotype Manifest In

Detailed Description

Statement

Reference

Mutant embryos derived from homozygous female germline clones have a "lawn of denticles" cuticle phenotype.

(Thompson, 2004)

pygo^S28 mutant embryos that lack maternal and zygotic pygo function display a cuticle denticle lawn that is characteristic of a complete loss of wg signalling.

(Townsley et al., 2004)

Somatic clones of pygo^S28 homozygous cells in the wing pouch region of third instar wing discs are smaller on average than their wild-type twin spots (P<0.001). Mutant wing pouch clones are associated with increased apoptosis marker expression. When these clones fall in the notum region of this disc there is no difference between mutant clone and twin spot size.

(Giraldez and Cohen, 2003)

Mutant embryos derived from homozygous female germline clones (lacking both maternal and zygotic pygo function) are short and lack the naked stretches that are normally interspersed between the ventral denticle belts ("lawn of denticles" phenotype). The middle gut constriction is missing. Homozygous clones result in loss of wing tissue, loss of the distal leg, dorsal duplication of the distal leg segments, nicking of the wing and wing margin defects.

(Thompson et al., 2002)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Suppressed by

Statement

Reference

pygo^S28 has denticle | ectopic phenotype, suppressible by lgs::pygo^{ΔPHD.HD2.αTub84B}

(Thompson, 2004)

pygo^S28 has denticle | ectopic phenotype, suppressible by pan::pygo^{pygoΔPHD-ΔNTCF.UAS.Tag:HA}/Scer\GAL4^da.G32

(Thompson, 2004)

pygo^S28 has embryonic/first instar larval cuticle phenotype, suppressible by lgs::pygo^{ΔPHD.HD2.αTub84B}

(Thompson, 2004)

pygo^S28 has embryonic/first instar larval cuticle phenotype, suppressible by pan::pygo^{pygoΔPHD-ΔNTCF.UAS.Tag:HA}/Scer\GAL4^da.G32

(Thompson, 2004)

pygo^S28 has embryonic/first instar larval cuticle | maternal effect phenotype, suppressible by Scer\GAL4^da.G32/arm^{UAS.Tag:MYC,Tag:NLS(SV40-largeT)}

(Townsley et al., 2004)

NOT suppressed by

Statement

Reference

pygo^S28 has denticle | ectopic phenotype, non-suppressible by Scer\GAL4^da.G32/arm::pan^{armC.ΔNTCF.UAS.Tag:HA}

(Thompson, 2004)

pygo^S28 has embryonic/first instar larval cuticle phenotype, non-suppressible by Scer\GAL4^da.G32/arm::pan^{armC.ΔNTCF.UAS.Tag:HA}

(Thompson, 2004)

pygo^S28 has embryonic/first instar larval cuticle | germline clone phenotype, non-suppressible by arm^{S10.UAS.Tag:MYC}/Scer\GAL4^arm.PS

(Thompson et al., 2002)

Suppressor of

Statement

Reference

pygo[+]/pygo^S28 is a suppressor of phenotype of arm^S56F.GMR

(Thompson et al., 2002)

Additional Comments

Genetic Interactions

Statement

Reference

The "lawn of denticles" cuticle phenotype seen in pygo^S28 embryos is substantially rescued by expression of pan::pygo^{pygoΔPHD-ΔNTCF.Scer\UAS.T:Ivir\HA1} under the control of Scer\GAL4^da.G32; a segmented pattern of denticle bands and naked cuticle is restored. The "lawn of denticles" cuticle phenotype seen in pygo^S28 embryos is not rescued by expression of arm::pan^{armC.ΔNTCF.Scer\UAS.T:Ivir\HA1} under the control of Scer\GAL4^da.G32. The "lawn of denticles" cuticle phenotype seen in pygo^S28 embryos is rescued by lgs::pygo^{ΔPHD.HD2.αTub84B}.

(Thompson, 2004)

Overexpression of arm^{Scer\UAS.T:Hsap\MYC,T:SV40\nls2}, under the regulation of Scer\GAL4^da.G32 suppresses the mutant phenotype of pygo^S28 embryos which lack both maternal and zygotic pygo function. The degree of suppression varies very little between individual embryos, and is essentially the same as that provided by zygotic pygo expressed from the paternal wild-type allele.

(Townsley et al., 2004)

RpS3⁺; pygo^S28 homozygous clones in a RpS3²/+ background are 57% the size of RpS3⁺; pygo⁺ homozygous clones in a RpS3²/+ background; i.e.- pygo^S28 clones still have a growth disadvantage even when cell competition is suppressed.

(Giraldez and Cohen, 2003)

Expression of arm^{S10.Scer\UAS.T:Hsap\MYC} under the control of Scer\GAL4^arm.PS has no effect on the cuticle phenotype of pygo^S28 embryos derived from pygo^S28 germline clones.

(Thompson et al., 2002)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Fails to complement

pygo^S123

(Cook, 2006.1.31)

Rescued by

pygo^S28 is rescued by pygo^UAS.Tag:HA/Scer\GAL4^da.G32

(Thompson, 2004)

pygo^S28 is rescued by pygo^UAS.Tag:HA/Scer\GAL4^da.G32